General information | |||
TC004664 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 25266..27722 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC004664 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03808 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC004664 RA
MNELEKAES DCAKKAELV KSLEKQLAD AKAAYAKAS STLEELKRL KSLEENRKR IALTEDTLS DWLEEKFPW SAQLKSLLK EKFKFDHFR AKQLAAINA TLSKKDVLL VMPTGGGKS LVFQLPALI DPGFTLVVT PLVSLIQDQ LVALEKFKI EAATIHAST SKSEIKEIY DSMTSKKSA LKLLYVTPE WIARSKKFM SVLKKCYEA KRVARVAID EVHCCSTWG HDFRPDYPY LGLVRSLLE KVPFLGLTA TATMSVLND VQTMLNLEN CLIITAPFN RPNLFYQVL PKPATKDGV LNYLEKLMT EKYAGQSGI IYTNTINEC ISLATALKG SNLRVAPFH AQLEPEQKK KIHRKWLDN TYQAVVATV AFGMGIDKP DVRFVIHHV IPRSMESLY QESGRAGRD GKRADCIVL FSLNDYLKI SAMSTSLVE EKKAQQVLE YCVERLRCR RSIIATYFE QVWETSDCN KMCDICKRD VKIPAYYDL TQICKDLHS LIDQAKGFD EKLTLIKLI DAWYQAGLK KFRLSNVEK PKMSKEHAE LVIAFLLLE KYLAIDKGY TMYSTVSYI VKGRAVGDE KVEMLASDG MKIPKRLKE EGDGSPAKK KPRTD
- >TC004664 RA
atgaacgaa ttagaaaaa gctgaaagt gattgtgcc aaaaaggct gagttggta aagtcttta gaaaagcaa ctagcggac gctaaagcg gcatacgcg aaggccagt agcacctta gaagagtta aaacggtta aaatcgctt gaagaaaat cgcaagaga atagcctta actgaagac accttgagc gactggctt gaggagaag tttccctgg tcggcccaa ctcaaatca ctgctgaag gaaaaattt aaatttgac catttccgg gccaaacaa ctcgccgcc atcaatgcc accctctcc aaaaaggac gtccttctt gtcatgcca acggggggt ggaaaatca ctagtcttc caacttccc gccctcata gaccccggt tttaccctg gtggtcacc cccttagtg tcgctaatc caggaccag ttagtcgcc ttagaaaaa ttcaaaatt gaagccgcc accatccac gcaagtacc tcaaaaagt gaaattaag gagatttac gattcaatg actagtaaa aaatcggca ttgaaattg ctctacgta acccctgag tggatcgct cgcagtaaa aaatttatg tcggttttg aaaaagtgc tacgaggcg aagcgagtg gctcgcgtt gcgattgac gaggtgcat tgttgctca acctggggg cacgatttc cgccctgat tacccctac ttggggcta gttcgctcc ctgctggag aaagtgcct tttttgggg ctgacggcg accgccaca atgtcggtt ttaaacgat gtgcaaaca atgctgaat cttgagaat tgtctcatt attacagcg ccttttaat cgaccgaat ttgttttac caagttttg ccgaaaccg gctactaaa gacggggtt ttaaattat ttagagaaa ctaatgact gagaaatac gccggtcag agtggcatt atttatact aataccata aatgaatgt attagttta gcaacagct ttaaaaggc tcgaattta cgcgtggcc ccatttcat gcgcaattg gagcccgaa caaaagaaa aaaatacac agaaagtgg ttagataac acgtatcaa gctgtggtc gcaactgta gcattcgga atggggatt gataaacct gacgtcagg tttgtaatt caccatgtg attcccaga agcatggaa agcctgtat caggagagt ggtagagct ggacgcgac ggcaaacgc gccgattgc atagtccta tttagctta aacgattat ttaaaaatt tcagccatg tccacttcg ctagtagaa gaaaaaaag gcacaacaa gtactagaa tattgcgtt gagcgccta cgatgtcga cgctcaatt atagctaca tattttgag caagtttgg gaaactagc gattgtaac aaaatgtgc gatatttgc aaacgcgac gtaaaaatt cccgcctat tatgaccta acgcagata tgtaaagac ctacactca ttgatagac caagctaaa gggttcgac gagaaatta actctaatt aaactgata gatgcgtgg tatcaagcc ggtctgaaa aaattccga ctgtcgaac gtggaaaaa ccgaaaatg tcgaaagag catgccgag ctcgttatc gccttcctt ttgctagaa aaataccta gcaattgat aaggggtac accatgtat tccacggtt tcctacatc gtcaaaggg agggcagtc ggtgatgaa aaagtcgaa atgttggct tcggacggc atgaaaata ccgaaaaga ctgaaggag gaaggcgat ggatctccc gcaaagaaa aaaccaaga actgactaa
- >TC004664 RA
gtaacgcag tttccggca actatatga aaaaataac ctaattttt tcgcacttt ttccaaaaa acagtatta tcaattgtg ataatttaa ccggctttc cgataagtt tttacattt cctagtgtt ggtttttag atattgtag cacaataaa aaaatgaac gaattagaa aaagctgaa agtgattgt gccaaaaag gctgagttg gtaaagtct ttagaaaag caactagcg gacgctaaa gcggcatac gcgaaggcc agtagcacc ttagaagag ttaaaacgg ttaaaatcg cttgaagaa aatcgcaag agaatagcc ttaactgaa gacaccttg agcgactgg cttgaggag aagtttccc tggtcggcc caactcaaa tcactgctg aaggaaaaa tttaaattt gaccatttc cgggccaaa caactcgcc gccatcaat gccaccctc tccaaaaag gacgtcctt cttgtcatg ccaacgggg ggtggaaaa tcactagtc ttccaactt cccgccctc atagacccc ggttttacc ctggtggtc accccctta gtgtcgcta atccaggac cagttagtc gccttagaa aaattcaaa attgaagcc gccaccatc cacgcaagt acctcaaaa agtgaaatt aaggagatt tacgattca atgactagt aaaaaatcg gcattgaaa ttgctctac gtaacccct gagtggatc gctcgcagt aaaaaattt atgtcggtt ttgaaaaag tgctacgag gcgaagcga gtggctcgc gttgcgatt gacgaggtg cattgttgc tcaacctgg gggcacgat ttccgccct gattacccc tacttgggg ctagttcgc tccctgctg gagaaagtg ccttttttg gggctgacg gcgaccgcc acaatgtcg gttttaaac gatgtgcaa acaatgctg aatcttgag aattgtctc attattaca gcgcctttt aatcgaccg aatttgttt taccaagtt ttgccgaaa ccggctact aaagacggg gttttaaat tatttagag aaactaatg actgagaaa tacgccggt cagagtggc attatttat actaatacc ataaatgaa tgtattagt ttagcaaca gctttaaaa ggctcgaat ttacgcgtg gccccattt catgcgcaa ttggagccc gaacaaaag aaaaaaata cacagaaag tggttagat aacacgtat caagctgtg gtcgcaact gtagcattc ggaatgggg attgataaa cctgacgtc aggtttgta attcaccat gtgattccc agaagcatg gaaagcctg tatcaggag agtggtaga gctggacgc gacggcaaa cgcgccgat tgcatagtc ctatttagc ttaaacgat tatttaaaa atttcagcc atgtccact tcgctagta gaagaaaaa aaggcacaa caagtacta gaatattgc gttgagcgc ctacgatgt cgacgctca attatagct acatatttt gagcaagtt tgggaaact agcgattgt aacaaaatg tgcgatatt tgcaaacgc gacgtaaaa attcccgcc tattatgac ctaacgcag atatgtaaa gacctacac tcattgata gaccaagct aaagggttc gacgagaaa ttaactcta attaaactg atagatgcg tggtatcaa gccggtctg aaaaaattc cgactgtcg aacgtggaa aaaccgaaa atgtcgaaa gagcatgcc gagctcgtt atcgccttc cttttgcta gaaaaatac ctagcaatt gataagggg tacaccatg tattccacg gtttcctac atcgtcaaa gggagggca gtcggtgat gaaaaagtc gaaatgttg gcttcggac ggcatgaaa ataccgaaa agactgaag gaggaaggc gatggatct cccgcaaag aaaaaacca agaactgac taactgtat ttattattt tcaatatag agttttata aagt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
