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Gene information
General information
TC004664 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 25266..27722
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004664
    iBeetle screen iB_03808

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004664 RA
      MNELEKAES DCAKKAELV KSLEKQLAD AKAAYAKAS 
      STLEELKRL KSLEENRKR IALTEDTLS DWLEEKFPW 
      SAQLKSLLK EKFKFDHFR AKQLAAINA TLSKKDVLL 
      VMPTGGGKS LVFQLPALI DPGFTLVVT PLVSLIQDQ 
      LVALEKFKI EAATIHAST SKSEIKEIY DSMTSKKSA 
      LKLLYVTPE WIARSKKFM SVLKKCYEA KRVARVAID 
      EVHCCSTWG HDFRPDYPY LGLVRSLLE KVPFLGLTA 
      TATMSVLND VQTMLNLEN CLIITAPFN RPNLFYQVL 
      PKPATKDGV LNYLEKLMT EKYAGQSGI IYTNTINEC 
      ISLATALKG SNLRVAPFH AQLEPEQKK KIHRKWLDN 
      TYQAVVATV AFGMGIDKP DVRFVIHHV IPRSMESLY 
      QESGRAGRD GKRADCIVL FSLNDYLKI SAMSTSLVE 
      EKKAQQVLE YCVERLRCR RSIIATYFE QVWETSDCN 
      KMCDICKRD VKIPAYYDL TQICKDLHS LIDQAKGFD 
      EKLTLIKLI DAWYQAGLK KFRLSNVEK PKMSKEHAE 
      LVIAFLLLE KYLAIDKGY TMYSTVSYI VKGRAVGDE 
      KVEMLASDG MKIPKRLKE EGDGSPAKK KPRTD
    CDS sequence
    • >TC004664 RA
      atgaacgaa ttagaaaaa gctgaaagt gattgtgcc 
      aaaaaggct gagttggta aagtcttta gaaaagcaa 
      ctagcggac gctaaagcg gcatacgcg aaggccagt 
      agcacctta gaagagtta aaacggtta aaatcgctt 
      gaagaaaat cgcaagaga atagcctta actgaagac 
      accttgagc gactggctt gaggagaag tttccctgg 
      tcggcccaa ctcaaatca ctgctgaag gaaaaattt 
      aaatttgac catttccgg gccaaacaa ctcgccgcc 
      atcaatgcc accctctcc aaaaaggac gtccttctt 
      gtcatgcca acggggggt ggaaaatca ctagtcttc 
      caacttccc gccctcata gaccccggt tttaccctg 
      gtggtcacc cccttagtg tcgctaatc caggaccag 
      ttagtcgcc ttagaaaaa ttcaaaatt gaagccgcc 
      accatccac gcaagtacc tcaaaaagt gaaattaag 
      gagatttac gattcaatg actagtaaa aaatcggca 
      ttgaaattg ctctacgta acccctgag tggatcgct 
      cgcagtaaa aaatttatg tcggttttg aaaaagtgc 
      tacgaggcg aagcgagtg gctcgcgtt gcgattgac 
      gaggtgcat tgttgctca acctggggg cacgatttc 
      cgccctgat tacccctac ttggggcta gttcgctcc 
      ctgctggag aaagtgcct tttttgggg ctgacggcg 
      accgccaca atgtcggtt ttaaacgat gtgcaaaca 
      atgctgaat cttgagaat tgtctcatt attacagcg 
      ccttttaat cgaccgaat ttgttttac caagttttg 
      ccgaaaccg gctactaaa gacggggtt ttaaattat 
      ttagagaaa ctaatgact gagaaatac gccggtcag 
      agtggcatt atttatact aataccata aatgaatgt 
      attagttta gcaacagct ttaaaaggc tcgaattta 
      cgcgtggcc ccatttcat gcgcaattg gagcccgaa 
      caaaagaaa aaaatacac agaaagtgg ttagataac 
      acgtatcaa gctgtggtc gcaactgta gcattcgga 
      atggggatt gataaacct gacgtcagg tttgtaatt 
      caccatgtg attcccaga agcatggaa agcctgtat 
      caggagagt ggtagagct ggacgcgac ggcaaacgc 
      gccgattgc atagtccta tttagctta aacgattat 
      ttaaaaatt tcagccatg tccacttcg ctagtagaa 
      gaaaaaaag gcacaacaa gtactagaa tattgcgtt 
      gagcgccta cgatgtcga cgctcaatt atagctaca 
      tattttgag caagtttgg gaaactagc gattgtaac 
      aaaatgtgc gatatttgc aaacgcgac gtaaaaatt 
      cccgcctat tatgaccta acgcagata tgtaaagac 
      ctacactca ttgatagac caagctaaa gggttcgac 
      gagaaatta actctaatt aaactgata gatgcgtgg 
      tatcaagcc ggtctgaaa aaattccga ctgtcgaac 
      gtggaaaaa ccgaaaatg tcgaaagag catgccgag 
      ctcgttatc gccttcctt ttgctagaa aaataccta 
      gcaattgat aaggggtac accatgtat tccacggtt 
      tcctacatc gtcaaaggg agggcagtc ggtgatgaa 
      aaagtcgaa atgttggct tcggacggc atgaaaata 
      ccgaaaaga ctgaaggag gaaggcgat ggatctccc 
      gcaaagaaa aaaccaaga actgactaa
    mRNA sequence
    • >TC004664 RA
      gtaacgcag tttccggca actatatga aaaaataac 
      ctaattttt tcgcacttt ttccaaaaa acagtatta 
      tcaattgtg ataatttaa ccggctttc cgataagtt 
      tttacattt cctagtgtt ggtttttag atattgtag 
      cacaataaa aaaatgaac gaattagaa aaagctgaa 
      agtgattgt gccaaaaag gctgagttg gtaaagtct 
      ttagaaaag caactagcg gacgctaaa gcggcatac 
      gcgaaggcc agtagcacc ttagaagag ttaaaacgg 
      ttaaaatcg cttgaagaa aatcgcaag agaatagcc 
      ttaactgaa gacaccttg agcgactgg cttgaggag 
      aagtttccc tggtcggcc caactcaaa tcactgctg 
      aaggaaaaa tttaaattt gaccatttc cgggccaaa 
      caactcgcc gccatcaat gccaccctc tccaaaaag 
      gacgtcctt cttgtcatg ccaacgggg ggtggaaaa 
      tcactagtc ttccaactt cccgccctc atagacccc 
      ggttttacc ctggtggtc accccctta gtgtcgcta 
      atccaggac cagttagtc gccttagaa aaattcaaa 
      attgaagcc gccaccatc cacgcaagt acctcaaaa 
      agtgaaatt aaggagatt tacgattca atgactagt 
      aaaaaatcg gcattgaaa ttgctctac gtaacccct 
      gagtggatc gctcgcagt aaaaaattt atgtcggtt 
      ttgaaaaag tgctacgag gcgaagcga gtggctcgc 
      gttgcgatt gacgaggtg cattgttgc tcaacctgg 
      gggcacgat ttccgccct gattacccc tacttgggg 
      ctagttcgc tccctgctg gagaaagtg ccttttttg 
      gggctgacg gcgaccgcc acaatgtcg gttttaaac 
      gatgtgcaa acaatgctg aatcttgag aattgtctc 
      attattaca gcgcctttt aatcgaccg aatttgttt 
      taccaagtt ttgccgaaa ccggctact aaagacggg 
      gttttaaat tatttagag aaactaatg actgagaaa 
      tacgccggt cagagtggc attatttat actaatacc 
      ataaatgaa tgtattagt ttagcaaca gctttaaaa 
      ggctcgaat ttacgcgtg gccccattt catgcgcaa 
      ttggagccc gaacaaaag aaaaaaata cacagaaag 
      tggttagat aacacgtat caagctgtg gtcgcaact 
      gtagcattc ggaatgggg attgataaa cctgacgtc 
      aggtttgta attcaccat gtgattccc agaagcatg 
      gaaagcctg tatcaggag agtggtaga gctggacgc 
      gacggcaaa cgcgccgat tgcatagtc ctatttagc 
      ttaaacgat tatttaaaa atttcagcc atgtccact 
      tcgctagta gaagaaaaa aaggcacaa caagtacta 
      gaatattgc gttgagcgc ctacgatgt cgacgctca 
      attatagct acatatttt gagcaagtt tgggaaact 
      agcgattgt aacaaaatg tgcgatatt tgcaaacgc 
      gacgtaaaa attcccgcc tattatgac ctaacgcag 
      atatgtaaa gacctacac tcattgata gaccaagct 
      aaagggttc gacgagaaa ttaactcta attaaactg 
      atagatgcg tggtatcaa gccggtctg aaaaaattc 
      cgactgtcg aacgtggaa aaaccgaaa atgtcgaaa 
      gagcatgcc gagctcgtt atcgccttc cttttgcta 
      gaaaaatac ctagcaatt gataagggg tacaccatg 
      tattccacg gtttcctac atcgtcaaa gggagggca 
      gtcggtgat gaaaaagtc gaaatgttg gcttcggac 
      ggcatgaaa ataccgaaa agactgaag gaggaaggc 
      gatggatct cccgcaaag aaaaaacca agaactgac 
      taactgtat ttattattt tcaatatag agttttata 
      aagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY