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Gene information
General information
TC004659 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 785316..850261
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004659
    iBeetle screen iB_03807
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004659 RA
      MVVKRKQHN RYLAIGIVY IATIISQAM TDDPRFAEP 
      IPNVTVALG RDASLPCVV ENLGTYKVA WIHIDRQMI 
      LTIHRHVIS RVPRFSVSH DNAKTWLLH VSSVQKEDR 
      GYYMCQVNT NPMISQVGY LQVVVPPNI IDAESTQST 
      VAVRENQNI SLTCKADGF PTPKIMWRR EDSQAITVE 
      RRKKVNVYD GEQLNLTRI SRTEMGAYL CIATNGVPP 
      SVSKRIIVD VEFSPMIWV PNQLVGAPA GTDVTIDCH 
      TEAYPRAIS YWVYDNVML LPTKKYGTE TTENSYRAH 
      MKLSVRNLQ PGDFGNYRC ISKNSLGET EGSIRLYEI 
      PMPSTPPRA TEMKSNSNK ESAGKRNIS RPIIPDERP 
      KVVMVSSSE KDVLQPGPA VETGLGLYT PPGPQGSGC 
      VSMARWAVP QFAAVILHC FLSGTFSLS QN
    CDS sequence
    • >TC004659 RA
      atggtggtg aagcggaaa cagcacaac cggtactta 
      gccatcggt attgtgtac atcgccact atcatcagt 
      caggcgatg acagatgat cctcgattc gcagagccc 
      atccctaac gttacagtg gccttagga agagacgct 
      agtttaccg tgtgttgta gaaaatctc ggtacatac 
      aaggtcgct tggatacac atagaccga caaatgatt 
      ttgacaatt caccgtcac gtcatctcc agagtgccg 
      cgattcagc gtgtcccac gataatgcc aagacgtgg 
      cttctgcac gtcagtagc gtacaaaaa gaagaccgg 
      ggttactac atgtgccaa gttaacaca aatcccatg 
      attagccaa gtaggatat ctgcaagta gtcgttcct 
      ccgaatatc attgatgca gaaagcacg caatccaca 
      gtggctgtt cgtgaaaac cagaatata agcttgacg 
      tgcaaggcg gacggtttt cccacgcct aaaatcatg 
      tggagaaga gaggatagt caagctata accgtcgaa 
      cgtcgtaag aaagtgaac gtatacgac ggggagcaa 
      ttgaatctc acccgcatc agcagaacg gaaatgggc 
      gcctatctt tgcatagct acgaatgga gtgcctcca 
      tctgtgagc aaacgaatc atcgtggac gttgaattt 
      tctccgatg atttgggtt ccaaatcag ttggtcggg 
      gcaccagcc ggcacggat gtcacaata gactgccat 
      acagaggca tacccaaga gccattagc tattgggta 
      tacgacaac gtcatgctc ctgcctacg aagaagtat 
      ggaactgaa acgactgaa aacagctac agggcgcac 
      atgaaattg tcagtgcga aacctccaa cccggagac 
      ttcggtaac tatcgatgc atctccaag aattctctt 
      ggggaaacg gaaggatcc atccgacta tacgaaata 
      cctatgcca tcaactcct ccgcgagcc accgaaatg 
      aaaagcaat tccaacaag gagagcgcg gggaagcgc 
      aacatcagc aggccgatt attccggac gaaaggccc 
      aaagttgta atggtatcc tcctcggag aaggacgtc 
      ttgcaacca gggcccgcc gtcgaaacc gggttggga 
      ctttacaca cctccaggg ccgcaagga tcaggatgt 
      gtcagcatg gcaaggtgg gcagtccct cagtttgca 
      gctgtgatt cttcactgt tttttgtcg ggcacgttt 
      tccctttct caaaattaa
    mRNA sequence
    • >TC004659 RA
      cgagtgatt tttcacgca cagatgttt tagtggcgc 
      taagccgga cccgtctca aggtgcgga cacgcaagt 
      gacttatgc gatggtggt gaagcggaa acagcacaa 
      ccggtactt agccatcgg tattgtgta catcgccac 
      tatcatcag tcaggcgat gacagatga tcctcgatt 
      cgcagagcc catccctaa cgttacagt ggccttagg 
      aagagacgc tagtttacc gtgtgttgt agaaaatct 
      cggtacata caaggtcgc ttggataca catagaccg 
      acaaatgat tttgacaat tcaccgtca cgtcatctc 
      cagagtgcc gcgattcag cgtgtccca cgataatgc 
      caagacgtg gcttctgca cgtcagtag cgtacaaaa 
      agaagaccg gggttacta catgtgcca agttaacac 
      aaatcccat gattagcca agtaggata tctgcaagt 
      agtcgttcc tccgaatat cattgatgc agaaagcac 
      gcaatccac agtggctgt tcgtgaaaa ccagaatat 
      aagcttgac gtgcaaggc ggacggttt tcccacgcc 
      taaaatcat gtggagaag agaggatag tcaagctat 
      aaccgtcga acgtcgtaa gaaagtgaa cgtatacga 
      cggggagca attgaatct cacccgcat cagcagaac 
      ggaaatggg cgcctatct ttgcatagc tacgaatgg 
      agtgcctcc atctgtgag caaacgaat catcgtgga 
      cgttgaatt ttctccgat gatttgggt tccaaatca 
      gttggtcgg ggcaccagc cggcacgga tgtcacaat 
      agactgcca tacagaggc atacccaag agccattag 
      ctattgggt atacgacaa cgtcatgct cctgcctac 
      gaagaagta tggaactga aacgactga aaacagcta 
      cagggcgca catgaaatt gtcagtgcg aaacctcca 
      acccggaga cttcggtaa ctatcgatg catctccaa 
      gaattctct tggggaaac ggaaggatc catccgact 
      atacgaaat acctatgcc atcaactcc tccgcgagc 
      caccgaaat gaaaagcaa ttccaacaa ggagagcgc 
      ggggaagcg caacatcag caggccgat tattccgga 
      cgaaaggcc caaagttgt aatggtatc ctcctcgga 
      gaaggacgt cttgcaacc agggcccgc cgtcgaaac 
      cgggttggg actttacac acctccagg gccgcaagg 
      atcaggatg tgtcagcat ggcaaggtg ggcagtccc 
      tcagtttgc agctgtgat tcttcactg ttttttgtc 
      gggcacgtt ttccctttc tcaaaatta atgtaccat 
      atcaaataa tccagctca aaaccaaca aaatacaat 
      tccatttgc tcagctttt tcctacatt ttccgtttt 
      tacgtaacc atcatcgga gcattccat tgccaacat 
      atcagtaat tgtaacgat tattttacg tcctaaatt 
      caaagcaaa acatatttt ttatataga catttacaa 
      gtgttatga tatcttacg cccgaccga gaatcacaa 
      ttcaaattt ccacgccgt tcagctgta cgctgacca 
      ttatttagg taagtaaac atatctcaa caacgaaaa 
      ttggtaaca tatcatgac actccagcg gattattta 
      gaatggtta tttccactt tttgtgttg aatttttta 
      aacaaattt aaactagtt tagtcgttc cgcacacat 
      aaaatcgac aatataaat cgtacgcat ttgctttga 
      acacatgaa aaataaaat aaagcttgt ctcactgag 
      aaaaagctt gaaataaat agaacataa attcaaaag 
      cacgctgtt tgatcaagc gcaaaaagt ggagctaga 
      aattctaaa gaaaacaca ctatttaca tttagcact 
      cttttagta accacattt gaatttttt aatttcaag 
      caaatttcc gttgaaaag acaaatacc gtcaatagt 
      acatatcta cgcgtctat ggctatttt gtaaaagag 
      aagttcgcg taattgtgg atgcactga acagacaat 
      ctaaatcca cgtaaattc attcaagaa agaattgtt 
      acttgctaa acaatgtgc tgatttcaa agtgtgtta 
      gctctttaa aacttatat atttgtaca taaattata 
      acataaagc ttactgtaa aatatatgt atttagcgc 
      ctaagacaa caactctca ttttatctg cagtattat 
      acatgtttg taagaataa attaaacga tgttttgct 
      ttcactctc gtttccatc aaatgtgaa agtctaatt 
      ttgtacata gatttgtat agtgttatt ttgtattgg 
      aatcaaaat taactaaaa agcgccatt gtttacgcc 
      agaaaaacc gaaatgttt atttttatg ttcgccacc 
      tagtcagct cgataatga atttgtttg tgtgcaaac 
      tgtaataaa taacgaaaa attat