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Gene information
General information
TC004621 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6390533..6498196
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004621
    iBeetle screen iB_03800

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature mostly not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature mostly shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004621 RA
      MQSSGGNGF NALPESGTL PPMGPPAVP QNRFEPYFD 
      AMTPRNVTA LVGKSAYLS CRVRNLGNK TVSWIRHRD 
      IHILTVGSY TYTSDQRFQ ANHHPETDE WTLQIKWAQ 
      KRDAGIYEC QISTQPVRS YFVNLNVVV PTAQILGGP 
      DLHVDKGST INLTCSIRF SPEPPAYIF WYHRDDVIS 
      YDSNRGGVS VITEKGDVT TSFLLIQHA DISDSGKYS 
      CSPSNADVA SVRVHVLNG ERPAAMQTG SAGLSNSSL 
      NILALMIIS YIYTAIYLS LQLETKQTV AALR
    • >TC004621 RB
      MQSSGGNGF NALPESGTL PPMGPPAVP QNRFEPYFD 
      AMTPRNVTA LVGKSAYLS CRVRNLGNK TVSWIRHRD 
      IHILTVGSY TYTSDQRFQ ANHHPETDE WTLQIKWAQ 
      KRDAGIYEC QISTQPVRS YFVNLNVVV SEVPAGDEP 
      SREVANKKN CSSCSPGAR LFMYAIPTA QILGGPDLH 
      VDKGSTINL TCSIRFSPE PPAYIFWYH RDDVISYDS 
      NRGGVSVIT EKGDVTTSF LLIQHADIS DSGKYSCSP 
      SNADVASVR VHVLNGERP AAMQTGSAG LSNSSLNIL 
      ALMIISYIY TAIYLSLQL ETKQTVAAL R
    CDS sequence
    • >TC004621 RA
      atgcaaagt tctggtggt aacggtttt aacgccctt 
      ccagagagc ggcactctg ccgcctatg ggaccacct 
      gcagttcca caaaatcgc tttgaacct tattttgac 
      gcaatgact ccacgcaat gtaacagct cttgttgga 
      aaatcggct tatctcagc tgccgcgta cgaaatttg 
      ggaaataaa acggtttcg tggattcgt catcgggat 
      attcacatt ttaactgtc ggcagttac acatacacg 
      agcgaccag agatttcag gcgaatcat caccctgaa 
      accgacgaa tggacttta cagatcaag tgggcacaa 
      aagagagat gccggaatt tacgagtgt caaattagc 
      acacaacca gtgaggagc tatttcgtt aaccttaat 
      gttgtcgtt cccacagcg cagatactt gggggacct 
      gatctgcat gtggataaa ggtagcacc atcaacttg 
      acatgttcg atccggttc agcccggaa ccgccggct 
      tacatcttc tggtaccac cgagacgac gtaataagt 
      tacgattcg aacagaggc ggcgtgagc gtcatcacc 
      gaaaaggga gatgtgact acaagtttc ttgttaatc 
      cagcatgct gacatttca gactccggc aaatattct 
      tgcagtccg tcgaacgca gatgttgca agcgtccga 
      gtacacgtc ttgaacggg gagagacct gccgctatg 
      caaaccgga tcagcggga ctgtcgaac agttctttg 
      aatatcctc gccctaatg atcatttct tacatttac 
      accgctatc tatttgagt ctccaactt gaaaccaaa 
      caaacggtg gctgcctta aggtag
    • >TC004621 RB
      atgcaaagt tctggtggt aacggtttt aacgccctt 
      ccagagagc ggcactctg ccgcctatg ggaccacct 
      gcagttcca caaaatcgc tttgaacct tattttgac 
      gcaatgact ccacgcaat gtaacagct cttgttgga 
      aaatcggct tatctcagc tgccgcgta cgaaatttg 
      ggaaataaa acggtttcg tggattcgt catcgggat 
      attcacatt ttaactgtc ggcagttac acatacacg 
      agcgaccag agatttcag gcgaatcat caccctgaa 
      accgacgaa tggacttta cagatcaag tgggcacaa 
      aagagagat gccggaatt tacgagtgt caaattagc 
      acacaacca gtgaggagc tatttcgtt aaccttaat 
      gttgtcgtt tcagaggtc ccagccggc gacgagccc 
      tcccgtgaa gtagcgaat aaaaagaat tgtagtagt 
      tgttcccca ggtgccagg ttgtttatg tatgccatt 
      cccacagcg cagatactt gggggacct gatctgcat 
      gtggataaa ggtagcacc atcaacttg acatgttcg 
      atccggttc agcccggaa ccgccggct tacatcttc 
      tggtaccac cgagacgac gtaataagt tacgattcg 
      aacagaggc ggcgtgagc gtcatcacc gaaaaggga 
      gatgtgact acaagtttc ttgttaatc cagcatgct 
      gacatttca gactccggc aaatattct tgcagtccg 
      tcgaacgca gatgttgca agcgtccga gtacacgtc 
      ttgaacggg gagagacct gccgctatg caaaccgga 
      tcagcggga ctgtcgaac agttctttg aatatcctc 
      gccctaatg atcatttct tacatttac accgctatc 
      tatttgagt ctccaactt gaaaccaaa caaacggtg 
      gctgcctta aggtag
    mRNA sequence
    • >TC004621 RA
      atttacgac actccatcc acaatttac ctgttacga 
      acacgcgga tattttcat ttttaaata actcactcc 
      aagtggaac aactttccg tgacaacac ccccgtccg 
      gggcattct cccgcctct cagcggacg caacaatgt 
      gccctcttt tattttgca aattcgtgg ataattttc 
      gaaatcaaa cactgtgaa tccaataaa gcaggcgtg 
      gcctaattt taactgcaa cagtgttgt cgttcgaat 
      gcaacttgg attttagtg gcagtgaat ggaaatgtg 
      agttttcag cgagatact ggacatttt gccgaaatg 
      ggagacatt atggtgcca taccttctg gcggttgct 
      atggtcttt tccaacaca gggtcgatt ctggtaaac 
      ggagggatg caaagttct ggtggtaac ggttttaac 
      gcccttcca gagagcggc actctgccg cctatggga 
      ccacctgca gttccacaa aatcgcttt gaaccttat 
      tttgacgca atgactcca cgcaatgta acagctctt 
      gttggaaaa tcggcttat ctcagctgc cgcgtacga 
      aatttggga aataaaacg gtttcgtgg attcgtcat 
      cgggatatt cacatttta actgtcggc agttacaca 
      tacacgagc gaccagaga tttcaggcg aatcatcac 
      cctgaaacc gacgaatgg actttacag atcaagtgg 
      gcacaaaag agagatgcc ggaatttac gagtgtcaa 
      attagcaca caaccagtg aggagctat ttcgttaac 
      cttaatgtt gtcgttccc acagcgcag atacttggg 
      ggacctgat ctgcatgtg gataaaggt agcaccatc 
      aacttgaca tgttcgatc cggttcagc ccggaaccg 
      ccggcttac atcttctgg taccaccga gacgacgta 
      ataagttac gattcgaac agaggcggc gtgagcgtc 
      atcaccgaa aagggagat gtgactaca agtttcttg 
      ttaatccag catgctgac atttcagac tccggcaaa 
      tattcttgc agtccgtcg aacgcagat gttgcaagc 
      gtccgagta cacgtcttg aacggggag agacctgcc 
      gctatgcaa accggatca gcgggactg tcgaacagt 
      tctttgaat atcctcgcc ctaatgatc atttcttac 
      atttacacc gctatctat ttgagtctc caacttgaa 
      accaaacaa acggtggct gccttaagg tagccattt 
      ggcgccaat gtcaataaa tgtatataa attaagtaa 
      aagcgcgag ttctcaacg acttataca agaaaaaat 
      atataaaaa tacactaaa tcactgtac agaaacatt 
      aaatttaat caagtgttt caaatttct gtgttctag 
      agtttacct acgttgtgt gctgttatc ggatcaaat 
      atttggatc agtaattta tgttagacg aacaaattt 
      attttacat tgttctgtt ccgcagtaa tagtcatac 
      tgttgaatt aacatgttg gtgtttttc gtgttgcat 
      accacttgg agtaactta aagtgtcgg tgagaacca 
      aaattgaaa cacttaaag ttatgaatg ttaattgtt 
      attttaaag gttaaccat agtgtaaat ataacattt 
      taaactgga ttaggcgat agatgtaaa attgagtaa 
      atagaatca taacgaggg aaggttttt attggggaa 
      gtttgtgtg aaatcatag agaaacaaa cagtaactt 
      ccccggcaa gtgaaatat atattttat ttgttgttg 
      acatgcaag ctataactt tgtattagg aaaatgaag 
      gaaagtttt attggatga acaaaagtg aatagactt 
      tcagtgaca cttttcaat gtgtttaaa aaaggtgta 
      taattaagg atttcacgt agaaaactt cttgttgcc 
      agaggaaag ttttgttaa ttattctcc ccaggcgaa 
      ctggaataa attttatcc actcaacca gtatggata 
      tttatatat taacgggtt attaatttc gcttcaaca 
      gggtatacg taattattt cggttattt ttaaaatgt 
      ataacattg taattattc atgttcatg tagattggc 
      cttttgttg acggtgaaa caataaatc tcaacaaaa 
      tggattaat cattattcg ataattatt tcccagttt 
      gaagtgact gcagtgact ttaacgcat tttggaaga 
      taatgtaaa ttactgtaa atactttag atagaacta 
      acatataag ccactgctg tgtataaaa tacaggggc 
      ggcgctaga cctttttgt attttttct atttccttg 
      tcataagtc gtgcatcaa tgccgattt tcccaaaaa 
      aacaaaacg ctagcgacg tcattgtta tcgaataca 
      atgaaaaat cagtaaagc aagttacta agatagggt 
      aaattagat ctctttgtt ttactgttc taaatgtat 
      tcaaattac agaagtacc tatatcgac aattcatta 
      tctcaaaat gtgtagtgt gtaatttat attactgta 
      aagtttatt aataaataa acacccatt ttaaaa
    • >TC004621 RB
      atttacgac actccatcc acaatttac ctgttacga 
      acacgcgga tattttcat ttttaaata actcactcc 
      aagtggaac aactttccg tgacaacac ccccgtccg 
      gggcattct cccgcctct cagcggacg caacaatgt 
      gccctcttt tattttgca aattcgtgg ataattttc 
      gaaatcaaa cactgtgaa tccaataaa gcaggcgtg 
      gcctaattt taactgcaa cagtgttgt cgttcgaat 
      gcaacttgg attttagtg gcagtgaat ggaaatgtg 
      agttttcag cgagatact ggacatttt gccgaaatg 
      ggagacatt atggtgcca taccttctg gcggttgct 
      atggtcttt tccaacaca gggtcgatt ctggtaaac 
      ggagggatg caaagttct ggtggtaac ggttttaac 
      gcccttcca gagagcggc actctgccg cctatggga 
      ccacctgca gttccacaa aatcgcttt gaaccttat 
      tttgacgca atgactcca cgcaatgta acagctctt 
      gttggaaaa tcggcttat ctcagctgc cgcgtacga 
      aatttggga aataaaacg gtttcgtgg attcgtcat 
      cgggatatt cacatttta actgtcggc agttacaca 
      tacacgagc gaccagaga tttcaggcg aatcatcac 
      cctgaaacc gacgaatgg actttacag atcaagtgg 
      gcacaaaag agagatgcc ggaatttac gagtgtcaa 
      attagcaca caaccagtg aggagctat ttcgttaac 
      cttaatgtt gtcgtttca gaggtccca gccggcgac 
      gagccctcc cgtgaagta gcgaataaa aagaattgt 
      agtagttgt tccccaggt gccaggttg tttatgtat 
      gccattccc acagcgcag atacttggg ggacctgat 
      ctgcatgtg gataaaggt agcaccatc aacttgaca 
      tgttcgatc cggttcagc ccggaaccg ccggcttac 
      atcttctgg taccaccga gacgacgta ataagttac 
      gattcgaac agaggcggc gtgagcgtc atcaccgaa 
      aagggagat gtgactaca agtttcttg ttaatccag 
      catgctgac atttcagac tccggcaaa tattcttgc 
      agtccgtcg aacgcagat gttgcaagc gtccgagta 
      cacgtcttg aacggggag agacctgcc gctatgcaa 
      accggatca gcgggactg tcgaacagt tctttgaat 
      atcctcgcc ctaatgatc atttcttac atttacacc 
      gctatctat ttgagtctc caacttgaa accaaacaa 
      acggtggct gccttaagg tagccattt ggcgccaat 
      gtcaataaa tgtatataa attaagtaa aagcgcgag 
      ttctcaacg acttataca agaaaaaat atataaaaa 
      tacactaaa tcactgtac agaaacatt aaatttaat 
      caagtgttt caaatttct gtgttctag agtttacct 
      acgttgtgt gctgttatc ggatcaaat atttggatc 
      agtaattta tgttagacg aacaaattt attttacat 
      tgttctgtt ccgcagtaa tagtcatac tgttgaatt 
      aacatgttg gtgtttttc gtgttgcat accacttgg 
      agtaactta aagtgtcgg tgagaacca aaattgaaa 
      cacttaaag ttatgaatg ttaattgtt attttaaag 
      gttaaccat agtgtaaat ataacattt taaactgga 
      ttaggcgat agatgtaaa attgagtaa atagaatca 
      taacgaggg aaggttttt attggggaa gtttgtgtg 
      aaatcatag agaaacaaa cagtaactt ccccggcaa 
      gtgaaatat atattttat ttgttgttg acatgcaag 
      ctataactt tgtattagg aaaatgaag gaaagtttt 
      attggatga acaaaagtg aatagactt tcagtgaca 
      cttttcaat gtgtttaaa aaaggtgta taattaagg 
      atttcacgt agaaaactt cttgttgcc agaggaaag 
      ttttgttaa ttattctcc ccaggcgaa ctggaataa 
      attttatcc actcaacca gtatggata tttatatat 
      taacgggtt attaatttc gcttcaaca gggtatacg 
      taattattt cggttattt ttaaaatgt ataacattg 
      taattattc atgttcatg tagattggc cttttgttg 
      acggtgaaa caataaatc tcaacaaaa tggattaat 
      cattattcg ataattatt tcccagttt gaagtgact 
      gcagtgact ttaacgcat tttggaaga taatgtaaa 
      ttactgtaa atactttag atagaacta acatataag 
      ccactgctg tgtataaaa tacaggggc ggcgctaga 
      cctttttgt attttttct atttccttg tcataagtc 
      gtgcatcaa tgccgattt tcccaaaaa aacaaaacg 
      ctagcgacg tcattgtta tcgaataca atgaaaaat 
      cagtaaagc aagttacta agatagggt aaattagat 
      ctctttgtt ttactgttc taaatgtat tcaaattac 
      agaagtacc tatatcgac aattcatta tctcaaaat 
      gtgtagtgt gtaatttat attactgta aagtttatt 
      aataaataa acacccatt ttaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY