| General information | |||
| TC004607 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 6674907..6677898 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004607 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03799 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
- >TC004607 RA
MASNNNQQN GWLSGLGMG FGGALSCVP EQQQQQQPG ASSDPNQFE QLDRTSVGS NTLKRNPKM ELSKNDLLK LVTYFEGEI QARDIAIAT LKSERLKQV VNVGRYRPA VASDPYIAL LRDSIADGP NAKPVVSEQ EMAVAAEQQ LQALEQLAL QQRRAHQHM INILKEAEA KHRKVIQEL EEEKCKHEH DTAQGDDIT YGLEMERTR LRQELEMER SARKKLEKE VKKQLEMAD EERARQKQI VLLLLAERK KIIVKYIEE RKRSEDLAQ ILSEEKSRI DSMAEGLEE ESKKSLQME AELEKQLHV FEADKKSLT QQLAIKEAR CQELEAELQ KLKADYELL KTRTNVSCD SAAPMISSV AKVVQPTAT VSSVPVSGP TTGIAQSVT PGQALRQTA IAPPVQMAT TKLMANAGP STQPPVTRS QPKPAFHAQ YQSPSAPQT PPKKGAVAG RGVPPPIPP NKPVIPCKQ TPARRPDAN ESEKKKTSS AGNKEAAPS SGESSRSGS QGIEILGQE LADFQQMLG SDDNQN
- >TC004607 RB
MASNNNQQN GWLSGLGMG FGGALSCVP EQQQQQQPG ASSDPNQFE QLDRTSVGS NTLKRNPKM ELSKNDLLK LVTYFEGEI QARDIAIAT LKSERLKQV VNVGRYRPA VASDPYIAL LRDSIADGP NAKPVVSEQ EMAVAAEQQ LQALEQLAL QQRRAHQHM INILKEAEA KHRKVIQEL EEEKCKHEH DTAQGDDIT YGLEMERTR LRQELEMER SARKKLEKE VKKQLEMAD EERARQKQI VLLLLAERK KIIVKYIEE RKRSEDLAQ ILSEEKSRI DSMAEGLEE ESKKSLQME AELEKQLHV FEADKKSLT QQLAIKEAR CQELEAELQ KLKADYELL KTRTNVSCD SAAPMISSV AKVVQPTAT VSSVPVSGP TTGIAQSVT PGQALRQTA IAPPVQMAT TKLMANAGP STQPPVTRS QPKPAFHAQ YQSPSAPQT PPKKGAVAG RGVPPPIPP NKPVIPCKQ TPARRPDAN ESEKKKTSS AGNKEAAPS SGESSRSGS QGIEILGQE LADFQQMFV TMATSNNT
- >TC004607 RA
atggcttct aataacaat cagcagaat ggatggttg agtggctta ggaatggga tttgggggc gccttaagt tgtgttccg gaacaacag cagcaacaa cagccaggg gcttcctca gatccaaat cagtttgaa caattagac aggactagt gtcggcagt aatactctt aagagaaat ccgaaaatg gagttatct aaaaacgat cttcttaaa ttagttacg tattttgaa ggtgagatc caagctaga gacatcgcg attgctacc ttgaagtct gagaggttg aaacaagta gtgaatgta ggacgttat agacccgct gtggcgtct gatccctac atagcttta cttagagac agtattgcc gatggacca aatgcaaaa cctgttgtc agtgaacag gaaatggct gttgcagct gaacaacag ttgcaagca ctagaacaa ttagcgctt caacaaaga cgagctcat caacatatg ataaatata ttaaaagaa gccgaagca aaacatagg aaagtcata caagaacta gaagaagaa aaatgtaaa cacgaacat gacactgct caaggtgat gatattact tatggttta gaaatggag aggactagg ttaagacaa gaattggaa atggagagg tctgcaagg aaaaaacta gaaaaagaa gttaaaaaa caattagaa atggctgac gaagaaaga gcaagacaa aaacaaatt gttctgctt ttgttggct gaaaggaaa aaaattata gtgaaatat atcgaggag agaaaaagg agtgaagat ttagcacaa atattatct gaagaaaag tcaaggatt gacagtatg gctgaaggt ttagaggaa gaaagcaaa aaatcactg caaatggag gctgaactt gaaaaacaa ctacacgtt tttgaagct gacaagaaa tcactaact caacaactt gccattaag gaagctaga tgtcaagaa ttggaagct gaattacaa aaattaaaa gctgattat gaattatta aaaactcga actaacgta tcttgtgat agtgcagct cctatgatt agtagtgta gctaaggtg gtacaaccg acggcaact gtttcaagt gttccagtt tcaggccct accacgggc attgcacag tctgtaaca ccaggccag gctttgcgt cagacagca atagcgccc ccggtgcaa atggcaaca actaagttg atggcaaat gcgggccct tcgacgcag ccaccagtg actcgatct caacctaaa ccagcattt cacgcccaa taccagtcg ccctctgcc ccccaaacc cctcccaag aagggggct gtggccggg cgtggcgtt cctccccca attccccca aacaaacct gtcattccc tgcaaacaa acgccagct cgacgaccg gacgctaac gaatccgag aaaaaaaaa acaagcagt gccggtaat aaggaagca gcgccatct tccggcgaa tcaagtcgt agtggctct caaggaata gaaatctta ggtcaagag cttgccgat tttcaacaa atgttgggg tctgacgat aatcagaat tga
- >TC004607 RB
atggcttct aataacaat cagcagaat ggatggttg agtggctta ggaatggga tttgggggc gccttaagt tgtgttccg gaacaacag cagcaacaa cagccaggg gcttcctca gatccaaat cagtttgaa caattagac aggactagt gtcggcagt aatactctt aagagaaat ccgaaaatg gagttatct aaaaacgat cttcttaaa ttagttacg tattttgaa ggtgagatc caagctaga gacatcgcg attgctacc ttgaagtct gagaggttg aaacaagta gtgaatgta ggacgttat agacccgct gtggcgtct gatccctac atagcttta cttagagac agtattgcc gatggacca aatgcaaaa cctgttgtc agtgaacag gaaatggct gttgcagct gaacaacag ttgcaagca ctagaacaa ttagcgctt caacaaaga cgagctcat caacatatg ataaatata ttaaaagaa gccgaagca aaacatagg aaagtcata caagaacta gaagaagaa aaatgtaaa cacgaacat gacactgct caaggtgat gatattact tatggttta gaaatggag aggactagg ttaagacaa gaattggaa atggagagg tctgcaagg aaaaaacta gaaaaagaa gttaaaaaa caattagaa atggctgac gaagaaaga gcaagacaa aaacaaatt gttctgctt ttgttggct gaaaggaaa aaaattata gtgaaatat atcgaggag agaaaaagg agtgaagat ttagcacaa atattatct gaagaaaag tcaaggatt gacagtatg gctgaaggt ttagaggaa gaaagcaaa aaatcactg caaatggag gctgaactt gaaaaacaa ctacacgtt tttgaagct gacaagaaa tcactaact caacaactt gccattaag gaagctaga tgtcaagaa ttggaagct gaattacaa aaattaaaa gctgattat gaattatta aaaactcga actaacgta tcttgtgat agtgcagct cctatgatt agtagtgta gctaaggtg gtacaaccg acggcaact gtttcaagt gttccagtt tcaggccct accacgggc attgcacag tctgtaaca ccaggccag gctttgcgt cagacagca atagcgccc ccggtgcaa atggcaaca actaagttg atggcaaat gcgggccct tcgacgcag ccaccagtg actcgatct caacctaaa ccagcattt cacgcccaa taccagtcg ccctctgcc ccccaaacc cctcccaag aagggggct gtggccggg cgtggcgtt cctccccca attccccca aacaaacct gtcattccc tgcaaacaa acgccagct cgacgaccg gacgctaac gaatccgag aaaaaaaaa acaagcagt gccggtaat aaggaagca gcgccatct tccggcgaa tcaagtcgt agtggctct caaggaata gaaatctta ggtcaagag cttgccgat tttcaacaa atgtttgtt actatggca actagcaat aatacttag
- >TC004607 RA
ggagttgtg ttggttatg tgagtataa attaatcga ttgttcacg ttaacctat aagagtgtt ttccaagtt gtaaattga ttgaacgat acgtttgaa atataatgt cgataataa aacaattat cgatcgaaa aaatggtag atcctccga tgttttagc ggaaaagtc tctgaaatt agggaagca ggaaaaaat ccgtcaaca tggcttcta ataacaatc agcagaatg gatggttga gtggcttag gaatgggat ttgggggcg ccttaagtt gtgttccgg aacaacagc agcaacaac agccagggg cttcctcag atccaaatc agtttgaac aattagaca ggactagtg tcggcagta atactctta agagaaatc cgaaaatgg agttatcta aaaacgatc ttcttaaat tagttacgt attttgaag gtgagatcc aagctagag acatcgcga ttgctacct tgaagtctg agaggttga aacaagtag tgaatgtag gacgttata gacccgctg tggcgtctg atccctaca tagctttac ttagagaca gtattgccg atggaccaa atgcaaaac ctgttgtca gtgaacagg aaatggctg ttgcagctg aacaacagt tgcaagcac tagaacaat tagcgcttc aacaaagac gagctcatc aacatatga taaatatat taaaagaag ccgaagcaa aacatagga aagtcatac aagaactag aagaagaaa aatgtaaac acgaacatg acactgctc aaggtgatg atattactt atggtttag aaatggaga ggactaggt taagacaag aattggaaa tggagaggt ctgcaagga aaaaactag aaaaagaag ttaaaaaac aattagaaa tggctgacg aagaaagag caagacaaa aacaaattg ttctgcttt tgttggctg aaaggaaaa aaattatag tgaaatata tcgaggaga gaaaaagga gtgaagatt tagcacaaa tattatctg aagaaaagt caaggattg acagtatgg ctgaaggtt tagaggaag aaagcaaaa aatcactgc aaatggagg ctgaacttg aaaaacaac tacacgttt ttgaagctg acaagaaat cactaactc aacaacttg ccattaagg aagctagat gtcaagaat tggaagctg aattacaaa aattaaaag ctgattatg aattattaa aaactcgaa ctaacgtat cttgtgata gtgcagctc ctatgatta gtagtgtag ctaaggtgg tacaaccga cggcaactg tttcaagtg ttccagttt caggcccta ccacgggca ttgcacagt ctgtaacac caggccagg ctttgcgtc agacagcaa tagcgcccc cggtgcaaa tggcaacaa ctaagttga tggcaaatg cgggccctt cgacgcagc caccagtga ctcgatctc aacctaaac cagcatttc acgcccaat accagtcgc cctctgccc cccaaaccc ctcccaaga agggggctg tggccgggc gtggcgttc ctcccccaa ttcccccaa acaaacctg tcattccct gcaaacaaa cgccagctc gacgaccgg acgctaacg aatccgaga aaaaaaaaa caagcagtg ccggtaata aggaagcag cgccatctt ccggcgaat caagtcgta gtggctctc aaggaatag aaatcttag gtcaagagc ttgccgatt ttcaacaaa tgttggggt ctgacgata atcagaatt gacctcaga agtccactt cttatgtga taattacaa aattaaatc tatacttga atagtgcat gagttgtta gagttattt ttttacttt ggcactgca actttagcc cagtaactt ttcttgggg cggagcaag actatgaga cggctacgt gtactaaat tttaatata aatctctaa ttgtgactg aaatatctc gttgtatca tatttttcg tgcgaaatt gatgtgtat tgcatttta tttaattaa ttatttttg cttttatat ttagggaca agtattgtt atcttgaat tgagctact taaatttgg ttgtatttt ttatacaaa ataaatttt tttttggta t
- >TC004607 RB
ggagttgtg ttggttatg tgagtataa attaatcga ttgttcacg ttaacctat aagagtgtt ttccaagtt gtaaattga ttgaacgat acgtttgaa atataatgt cgataataa aacaattat cgatcgaaa aaatggtag atcctccga tgttttagc ggaaaagtc tctgaaatt agggaagca ggaaaaaat ccgtcaaca tggcttcta ataacaatc agcagaatg gatggttga gtggcttag gaatgggat ttgggggcg ccttaagtt gtgttccgg aacaacagc agcaacaac agccagggg cttcctcag atccaaatc agtttgaac aattagaca ggactagtg tcggcagta atactctta agagaaatc cgaaaatgg agttatcta aaaacgatc ttcttaaat tagttacgt attttgaag gtgagatcc aagctagag acatcgcga ttgctacct tgaagtctg agaggttga aacaagtag tgaatgtag gacgttata gacccgctg tggcgtctg atccctaca tagctttac ttagagaca gtattgccg atggaccaa atgcaaaac ctgttgtca gtgaacagg aaatggctg ttgcagctg aacaacagt tgcaagcac tagaacaat tagcgcttc aacaaagac gagctcatc aacatatga taaatatat taaaagaag ccgaagcaa aacatagga aagtcatac aagaactag aagaagaaa aatgtaaac acgaacatg acactgctc aaggtgatg atattactt atggtttag aaatggaga ggactaggt taagacaag aattggaaa tggagaggt ctgcaagga aaaaactag aaaaagaag ttaaaaaac aattagaaa tggctgacg aagaaagag caagacaaa aacaaattg ttctgcttt tgttggctg aaaggaaaa aaattatag tgaaatata tcgaggaga gaaaaagga gtgaagatt tagcacaaa tattatctg aagaaaagt caaggattg acagtatgg ctgaaggtt tagaggaag aaagcaaaa aatcactgc aaatggagg ctgaacttg aaaaacaac tacacgttt ttgaagctg acaagaaat cactaactc aacaacttg ccattaagg aagctagat gtcaagaat tggaagctg aattacaaa aattaaaag ctgattatg aattattaa aaactcgaa ctaacgtat cttgtgata gtgcagctc ctatgatta gtagtgtag ctaaggtgg tacaaccga cggcaactg tttcaagtg ttccagttt caggcccta ccacgggca ttgcacagt ctgtaacac caggccagg ctttgcgtc agacagcaa tagcgcccc cggtgcaaa tggcaacaa ctaagttga tggcaaatg cgggccctt cgacgcagc caccagtga ctcgatctc aacctaaac cagcatttc acgcccaat accagtcgc cctctgccc cccaaaccc ctcccaaga agggggctg tggccgggc gtggcgttc ctcccccaa ttcccccaa acaaacctg tcattccct gcaaacaaa cgccagctc gacgaccgg acgctaacg aatccgaga aaaaaaaaa caagcagtg ccggtaata aggaagcag cgccatctt ccggcgaat caagtcgta gtggctctc aaggaatag aaatcttag gtcaagagc ttgccgatt ttcaacaaa tgtttgtta ctatggcaa ctagcaata atacttagg aataataat ataatttat ttgttgaca ggttggggt ctgacgata atcagaatt gacctcaga agtccactt cttatgtga taattacaa aattaaatc tatacttga atagtgcat gagttgtta gagttattt ttttacttt ggcactgca actttagcc cagtaactt ttcttgggg cggagcaag actatgaga cggctacgt gtactaaat tttaatata aatctctaa ttgtgactg aaatatctc gttgtatca tatttttcg tgcgaaatt gatgtgtat tgcatttta tttaattaa ttatttttg cttttatat ttagggaca agtattgtt atcttgaat tgagctact taaatttgg ttgtatttt ttatacaaa ataaatttt tttttggta t
