Status
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Gene information
General information
TC004607 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6674907..6677898
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004607
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03799

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004607 RA
      MASNNNQQN GWLSGLGMG FGGALSCVP EQQQQQQPG 
      ASSDPNQFE QLDRTSVGS NTLKRNPKM ELSKNDLLK 
      LVTYFEGEI QARDIAIAT LKSERLKQV VNVGRYRPA 
      VASDPYIAL LRDSIADGP NAKPVVSEQ EMAVAAEQQ 
      LQALEQLAL QQRRAHQHM INILKEAEA KHRKVIQEL 
      EEEKCKHEH DTAQGDDIT YGLEMERTR LRQELEMER 
      SARKKLEKE VKKQLEMAD EERARQKQI VLLLLAERK 
      KIIVKYIEE RKRSEDLAQ ILSEEKSRI DSMAEGLEE 
      ESKKSLQME AELEKQLHV FEADKKSLT QQLAIKEAR 
      CQELEAELQ KLKADYELL KTRTNVSCD SAAPMISSV 
      AKVVQPTAT VSSVPVSGP TTGIAQSVT PGQALRQTA 
      IAPPVQMAT TKLMANAGP STQPPVTRS QPKPAFHAQ 
      YQSPSAPQT PPKKGAVAG RGVPPPIPP NKPVIPCKQ 
      TPARRPDAN ESEKKKTSS AGNKEAAPS SGESSRSGS 
      QGIEILGQE LADFQQMLG SDDNQN
    • >TC004607 RB
      MASNNNQQN GWLSGLGMG FGGALSCVP EQQQQQQPG 
      ASSDPNQFE QLDRTSVGS NTLKRNPKM ELSKNDLLK 
      LVTYFEGEI QARDIAIAT LKSERLKQV VNVGRYRPA 
      VASDPYIAL LRDSIADGP NAKPVVSEQ EMAVAAEQQ 
      LQALEQLAL QQRRAHQHM INILKEAEA KHRKVIQEL 
      EEEKCKHEH DTAQGDDIT YGLEMERTR LRQELEMER 
      SARKKLEKE VKKQLEMAD EERARQKQI VLLLLAERK 
      KIIVKYIEE RKRSEDLAQ ILSEEKSRI DSMAEGLEE 
      ESKKSLQME AELEKQLHV FEADKKSLT QQLAIKEAR 
      CQELEAELQ KLKADYELL KTRTNVSCD SAAPMISSV 
      AKVVQPTAT VSSVPVSGP TTGIAQSVT PGQALRQTA 
      IAPPVQMAT TKLMANAGP STQPPVTRS QPKPAFHAQ 
      YQSPSAPQT PPKKGAVAG RGVPPPIPP NKPVIPCKQ 
      TPARRPDAN ESEKKKTSS AGNKEAAPS SGESSRSGS 
      QGIEILGQE LADFQQMFV TMATSNNT
    CDS sequence
    • >TC004607 RA
      atggcttct aataacaat cagcagaat ggatggttg 
      agtggctta ggaatggga tttgggggc gccttaagt 
      tgtgttccg gaacaacag cagcaacaa cagccaggg 
      gcttcctca gatccaaat cagtttgaa caattagac 
      aggactagt gtcggcagt aatactctt aagagaaat 
      ccgaaaatg gagttatct aaaaacgat cttcttaaa 
      ttagttacg tattttgaa ggtgagatc caagctaga 
      gacatcgcg attgctacc ttgaagtct gagaggttg 
      aaacaagta gtgaatgta ggacgttat agacccgct 
      gtggcgtct gatccctac atagcttta cttagagac 
      agtattgcc gatggacca aatgcaaaa cctgttgtc 
      agtgaacag gaaatggct gttgcagct gaacaacag 
      ttgcaagca ctagaacaa ttagcgctt caacaaaga 
      cgagctcat caacatatg ataaatata ttaaaagaa 
      gccgaagca aaacatagg aaagtcata caagaacta 
      gaagaagaa aaatgtaaa cacgaacat gacactgct 
      caaggtgat gatattact tatggttta gaaatggag 
      aggactagg ttaagacaa gaattggaa atggagagg 
      tctgcaagg aaaaaacta gaaaaagaa gttaaaaaa 
      caattagaa atggctgac gaagaaaga gcaagacaa 
      aaacaaatt gttctgctt ttgttggct gaaaggaaa 
      aaaattata gtgaaatat atcgaggag agaaaaagg 
      agtgaagat ttagcacaa atattatct gaagaaaag 
      tcaaggatt gacagtatg gctgaaggt ttagaggaa 
      gaaagcaaa aaatcactg caaatggag gctgaactt 
      gaaaaacaa ctacacgtt tttgaagct gacaagaaa 
      tcactaact caacaactt gccattaag gaagctaga 
      tgtcaagaa ttggaagct gaattacaa aaattaaaa 
      gctgattat gaattatta aaaactcga actaacgta 
      tcttgtgat agtgcagct cctatgatt agtagtgta 
      gctaaggtg gtacaaccg acggcaact gtttcaagt 
      gttccagtt tcaggccct accacgggc attgcacag 
      tctgtaaca ccaggccag gctttgcgt cagacagca 
      atagcgccc ccggtgcaa atggcaaca actaagttg 
      atggcaaat gcgggccct tcgacgcag ccaccagtg 
      actcgatct caacctaaa ccagcattt cacgcccaa 
      taccagtcg ccctctgcc ccccaaacc cctcccaag 
      aagggggct gtggccggg cgtggcgtt cctccccca 
      attccccca aacaaacct gtcattccc tgcaaacaa 
      acgccagct cgacgaccg gacgctaac gaatccgag 
      aaaaaaaaa acaagcagt gccggtaat aaggaagca 
      gcgccatct tccggcgaa tcaagtcgt agtggctct 
      caaggaata gaaatctta ggtcaagag cttgccgat 
      tttcaacaa atgttgggg tctgacgat aatcagaat 
      tga
    • >TC004607 RB
      atggcttct aataacaat cagcagaat ggatggttg 
      agtggctta ggaatggga tttgggggc gccttaagt 
      tgtgttccg gaacaacag cagcaacaa cagccaggg 
      gcttcctca gatccaaat cagtttgaa caattagac 
      aggactagt gtcggcagt aatactctt aagagaaat 
      ccgaaaatg gagttatct aaaaacgat cttcttaaa 
      ttagttacg tattttgaa ggtgagatc caagctaga 
      gacatcgcg attgctacc ttgaagtct gagaggttg 
      aaacaagta gtgaatgta ggacgttat agacccgct 
      gtggcgtct gatccctac atagcttta cttagagac 
      agtattgcc gatggacca aatgcaaaa cctgttgtc 
      agtgaacag gaaatggct gttgcagct gaacaacag 
      ttgcaagca ctagaacaa ttagcgctt caacaaaga 
      cgagctcat caacatatg ataaatata ttaaaagaa 
      gccgaagca aaacatagg aaagtcata caagaacta 
      gaagaagaa aaatgtaaa cacgaacat gacactgct 
      caaggtgat gatattact tatggttta gaaatggag 
      aggactagg ttaagacaa gaattggaa atggagagg 
      tctgcaagg aaaaaacta gaaaaagaa gttaaaaaa 
      caattagaa atggctgac gaagaaaga gcaagacaa 
      aaacaaatt gttctgctt ttgttggct gaaaggaaa 
      aaaattata gtgaaatat atcgaggag agaaaaagg 
      agtgaagat ttagcacaa atattatct gaagaaaag 
      tcaaggatt gacagtatg gctgaaggt ttagaggaa 
      gaaagcaaa aaatcactg caaatggag gctgaactt 
      gaaaaacaa ctacacgtt tttgaagct gacaagaaa 
      tcactaact caacaactt gccattaag gaagctaga 
      tgtcaagaa ttggaagct gaattacaa aaattaaaa 
      gctgattat gaattatta aaaactcga actaacgta 
      tcttgtgat agtgcagct cctatgatt agtagtgta 
      gctaaggtg gtacaaccg acggcaact gtttcaagt 
      gttccagtt tcaggccct accacgggc attgcacag 
      tctgtaaca ccaggccag gctttgcgt cagacagca 
      atagcgccc ccggtgcaa atggcaaca actaagttg 
      atggcaaat gcgggccct tcgacgcag ccaccagtg 
      actcgatct caacctaaa ccagcattt cacgcccaa 
      taccagtcg ccctctgcc ccccaaacc cctcccaag 
      aagggggct gtggccggg cgtggcgtt cctccccca 
      attccccca aacaaacct gtcattccc tgcaaacaa 
      acgccagct cgacgaccg gacgctaac gaatccgag 
      aaaaaaaaa acaagcagt gccggtaat aaggaagca 
      gcgccatct tccggcgaa tcaagtcgt agtggctct 
      caaggaata gaaatctta ggtcaagag cttgccgat 
      tttcaacaa atgtttgtt actatggca actagcaat 
      aatacttag
    mRNA sequence
    • >TC004607 RA
      ggagttgtg ttggttatg tgagtataa attaatcga 
      ttgttcacg ttaacctat aagagtgtt ttccaagtt 
      gtaaattga ttgaacgat acgtttgaa atataatgt 
      cgataataa aacaattat cgatcgaaa aaatggtag 
      atcctccga tgttttagc ggaaaagtc tctgaaatt 
      agggaagca ggaaaaaat ccgtcaaca tggcttcta 
      ataacaatc agcagaatg gatggttga gtggcttag 
      gaatgggat ttgggggcg ccttaagtt gtgttccgg 
      aacaacagc agcaacaac agccagggg cttcctcag 
      atccaaatc agtttgaac aattagaca ggactagtg 
      tcggcagta atactctta agagaaatc cgaaaatgg 
      agttatcta aaaacgatc ttcttaaat tagttacgt 
      attttgaag gtgagatcc aagctagag acatcgcga 
      ttgctacct tgaagtctg agaggttga aacaagtag 
      tgaatgtag gacgttata gacccgctg tggcgtctg 
      atccctaca tagctttac ttagagaca gtattgccg 
      atggaccaa atgcaaaac ctgttgtca gtgaacagg 
      aaatggctg ttgcagctg aacaacagt tgcaagcac 
      tagaacaat tagcgcttc aacaaagac gagctcatc 
      aacatatga taaatatat taaaagaag ccgaagcaa 
      aacatagga aagtcatac aagaactag aagaagaaa 
      aatgtaaac acgaacatg acactgctc aaggtgatg 
      atattactt atggtttag aaatggaga ggactaggt 
      taagacaag aattggaaa tggagaggt ctgcaagga 
      aaaaactag aaaaagaag ttaaaaaac aattagaaa 
      tggctgacg aagaaagag caagacaaa aacaaattg 
      ttctgcttt tgttggctg aaaggaaaa aaattatag 
      tgaaatata tcgaggaga gaaaaagga gtgaagatt 
      tagcacaaa tattatctg aagaaaagt caaggattg 
      acagtatgg ctgaaggtt tagaggaag aaagcaaaa 
      aatcactgc aaatggagg ctgaacttg aaaaacaac 
      tacacgttt ttgaagctg acaagaaat cactaactc 
      aacaacttg ccattaagg aagctagat gtcaagaat 
      tggaagctg aattacaaa aattaaaag ctgattatg 
      aattattaa aaactcgaa ctaacgtat cttgtgata 
      gtgcagctc ctatgatta gtagtgtag ctaaggtgg 
      tacaaccga cggcaactg tttcaagtg ttccagttt 
      caggcccta ccacgggca ttgcacagt ctgtaacac 
      caggccagg ctttgcgtc agacagcaa tagcgcccc 
      cggtgcaaa tggcaacaa ctaagttga tggcaaatg 
      cgggccctt cgacgcagc caccagtga ctcgatctc 
      aacctaaac cagcatttc acgcccaat accagtcgc 
      cctctgccc cccaaaccc ctcccaaga agggggctg 
      tggccgggc gtggcgttc ctcccccaa ttcccccaa 
      acaaacctg tcattccct gcaaacaaa cgccagctc 
      gacgaccgg acgctaacg aatccgaga aaaaaaaaa 
      caagcagtg ccggtaata aggaagcag cgccatctt 
      ccggcgaat caagtcgta gtggctctc aaggaatag 
      aaatcttag gtcaagagc ttgccgatt ttcaacaaa 
      tgttggggt ctgacgata atcagaatt gacctcaga 
      agtccactt cttatgtga taattacaa aattaaatc 
      tatacttga atagtgcat gagttgtta gagttattt 
      ttttacttt ggcactgca actttagcc cagtaactt 
      ttcttgggg cggagcaag actatgaga cggctacgt 
      gtactaaat tttaatata aatctctaa ttgtgactg 
      aaatatctc gttgtatca tatttttcg tgcgaaatt 
      gatgtgtat tgcatttta tttaattaa ttatttttg 
      cttttatat ttagggaca agtattgtt atcttgaat 
      tgagctact taaatttgg ttgtatttt ttatacaaa 
      ataaatttt tttttggta t
    • >TC004607 RB
      ggagttgtg ttggttatg tgagtataa attaatcga 
      ttgttcacg ttaacctat aagagtgtt ttccaagtt 
      gtaaattga ttgaacgat acgtttgaa atataatgt 
      cgataataa aacaattat cgatcgaaa aaatggtag 
      atcctccga tgttttagc ggaaaagtc tctgaaatt 
      agggaagca ggaaaaaat ccgtcaaca tggcttcta 
      ataacaatc agcagaatg gatggttga gtggcttag 
      gaatgggat ttgggggcg ccttaagtt gtgttccgg 
      aacaacagc agcaacaac agccagggg cttcctcag 
      atccaaatc agtttgaac aattagaca ggactagtg 
      tcggcagta atactctta agagaaatc cgaaaatgg 
      agttatcta aaaacgatc ttcttaaat tagttacgt 
      attttgaag gtgagatcc aagctagag acatcgcga 
      ttgctacct tgaagtctg agaggttga aacaagtag 
      tgaatgtag gacgttata gacccgctg tggcgtctg 
      atccctaca tagctttac ttagagaca gtattgccg 
      atggaccaa atgcaaaac ctgttgtca gtgaacagg 
      aaatggctg ttgcagctg aacaacagt tgcaagcac 
      tagaacaat tagcgcttc aacaaagac gagctcatc 
      aacatatga taaatatat taaaagaag ccgaagcaa 
      aacatagga aagtcatac aagaactag aagaagaaa 
      aatgtaaac acgaacatg acactgctc aaggtgatg 
      atattactt atggtttag aaatggaga ggactaggt 
      taagacaag aattggaaa tggagaggt ctgcaagga 
      aaaaactag aaaaagaag ttaaaaaac aattagaaa 
      tggctgacg aagaaagag caagacaaa aacaaattg 
      ttctgcttt tgttggctg aaaggaaaa aaattatag 
      tgaaatata tcgaggaga gaaaaagga gtgaagatt 
      tagcacaaa tattatctg aagaaaagt caaggattg 
      acagtatgg ctgaaggtt tagaggaag aaagcaaaa 
      aatcactgc aaatggagg ctgaacttg aaaaacaac 
      tacacgttt ttgaagctg acaagaaat cactaactc 
      aacaacttg ccattaagg aagctagat gtcaagaat 
      tggaagctg aattacaaa aattaaaag ctgattatg 
      aattattaa aaactcgaa ctaacgtat cttgtgata 
      gtgcagctc ctatgatta gtagtgtag ctaaggtgg 
      tacaaccga cggcaactg tttcaagtg ttccagttt 
      caggcccta ccacgggca ttgcacagt ctgtaacac 
      caggccagg ctttgcgtc agacagcaa tagcgcccc 
      cggtgcaaa tggcaacaa ctaagttga tggcaaatg 
      cgggccctt cgacgcagc caccagtga ctcgatctc 
      aacctaaac cagcatttc acgcccaat accagtcgc 
      cctctgccc cccaaaccc ctcccaaga agggggctg 
      tggccgggc gtggcgttc ctcccccaa ttcccccaa 
      acaaacctg tcattccct gcaaacaaa cgccagctc 
      gacgaccgg acgctaacg aatccgaga aaaaaaaaa 
      caagcagtg ccggtaata aggaagcag cgccatctt 
      ccggcgaat caagtcgta gtggctctc aaggaatag 
      aaatcttag gtcaagagc ttgccgatt ttcaacaaa 
      tgtttgtta ctatggcaa ctagcaata atacttagg 
      aataataat ataatttat ttgttgaca ggttggggt 
      ctgacgata atcagaatt gacctcaga agtccactt 
      cttatgtga taattacaa aattaaatc tatacttga 
      atagtgcat gagttgtta gagttattt ttttacttt 
      ggcactgca actttagcc cagtaactt ttcttgggg 
      cggagcaag actatgaga cggctacgt gtactaaat 
      tttaatata aatctctaa ttgtgactg aaatatctc 
      gttgtatca tatttttcg tgcgaaatt gatgtgtat 
      tgcatttta tttaattaa ttatttttg cttttatat 
      ttagggaca agtattgtt atcttgaat tgagctact 
      taaatttgg ttgtatttt ttatacaaa ataaatttt 
      tttttggta t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY