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Gene information
General information
TC004605 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 6708307..6709427
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004605
    iBeetle screen iB_03798

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004605 RA
      MCVGKVTSG KLQGPFGPP KTVKLVNES GKVPYQEDI 
LALSTYYTK VVLFTLDKW LKSFQDSIC YKILFHTTQ 
PAHVITALA VIMVSLMLI PSERKASLE SPLWSFIYM 
GSFSAHFGA QIWMTFISG LSLYFALPR HTFGNIQKV 
LFPKYFLLN SILSLITLY VFLRAHNYH LKSTEIAVQ 
VGAMTLCFL IELLIRLYL TPPLLKLMT MKNRIEAQA 
GVGMEIGKL VPGNLLNCP HYMAIHKAF RRVHMTIAI 
GNLVTMACT MLHLYYLSQ KLCVL
    CDS sequence
    • >TC004605 RA
      atgtgtgtt ggaaaagta acaagtggc aagttgcag 
gggcccttc gggcccccc aagaccgtg aaactagtc 
aacgaaagt ggaaaagtg ccctatcaa gaggatatc 
ctcgccctt tcgacgtac tacaccaaa gtggtgctc 
ttcactttg gataagtgg ctcaagtcc ttccaggac 
tcgatttgc tacaaaatt ctgttccac accactcaa 
ccagctcat gtgataaca gcactggct gtgataatg 
gtctcgctc atgctcatc ccttcggaa cgcaaagct 
tccctcgaa tcccctctg tggtcgttc atctacatg 
ggctcgttc agcgcccac ttcggggcc cagatctgg 
atgacgttc atttccggc ctgtctctc tacttcgcc 
ctgccgcgg cacaccttc ggcaacatc cagaaagtg 
ttatttcca aaatatttc ctcctcaat tctattttg 
agtttaata acactttac gtttttctc cgcgcccac 
aactaccat ctcaagtcg acggagatt gcagtgcag 
gtgggggcc atgaccttg tgttttttg atcgagtta 
ctgatccgt ctgtacctc acgcccccc ttgctgaag 
ctcatgacg atgaaaaac cgcatcgaa gcgcaggct 
ggggtcggc atggaaatc gggaagctg gtgccgggc 
aatttgctc aactgcccg cactacatg gccatacac 
aaggccttc aggagggtg cacatgacc atcgccatt 
gggaacctc gtcaccatg gcctgcacc atgctccac 
ctctactac ctgtcccag aagctatgt gtattataa
    mRNA sequence
    • >TC004605 RA
      gagtatcta taaaacacc taccctaaa gtgaaccgc 
tcattctga gaacgcgca ccgagtgca agccaaaga 
aacgaaaat gtgtgttgg aaaagtaac aagtggcaa 
gttgcaggg gcccttcgg gccccccaa gaccgtgaa 
actagtcaa cgaaagtgg aaaagtgcc ctatcaaga 
ggatatcct cgccctttc gacgtacta caccaaagt 
ggtgctctt cactttgga taagtggct caagtcctt 
ccaggactc gatttgcta caaaattct gttccacac 
cactcaacc agctcatgt gataacagc actggctgt 
gataatggt ctcgctcat gctcatccc ttcggaacg 
caaagcttc cctcgaatc ccctctgtg gtcgttcat 
ctacatggg ctcgttcag cgcccactt cggggccca 
gatctggat gacgttcat ttccggcct gtctctcta 
cttcgccct gccgcggca caccttcgg caacatcca 
gaaagtgtt atttccaaa atatttcct cctcaattc 
tattttgag tttaataac actttacgt ttttctccg 
cgcccacaa ctaccatct caagtcgac ggagattgc 
agtgcaggt gggggccat gaccttgtg ttttttgat 
cgagttact gatccgtct gtacctcac gcccccctt 
gctgaagct catgacgat gaaaaaccg catcgaagc 
gcaggctgg ggtcggcat ggaaatcgg gaagctggt 
gccgggcaa tttgctcaa ctgcccgca ctacatggc 
catacacaa ggccttcag gagggtgca catgaccat 
cgccattgg gaacctcgt caccatggc ctgcaccat 
gctccacct ctactacct gtcccagaa gctatgtgt 
attataaat tattattta ttgtaattt atgcgaata 
cgacagctt tatttaaag cgcctagtg atagcctat 
ttattgttt ttgtattta tccttttgt aataaagag 
taaaaaatt aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY