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Gene information
General information
TC004594 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7010768..7026470
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004594
    iBeetle screen iB_03795

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages partially shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule sometimes size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    gena triplet seta number increased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    vertex triplet vertex triplet posterior not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    wings sometimes blistered - Number of animals showing the phenotype: 2

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004594 RA
      MAFENGVHN VEVSAAFIE SLQTDFKNL ASESKKKYP 
      VIKESCEEA IVKLKAAVS TPQTSIYYV INQIIYPLS 
      QGCETKDVK LIKGCLQII QKLITHQLI DQKGALYIT 
      DTLWTLMES GIEEVKILQ SVTLLLTTN TVVHGDTLA 
      RTLVLCFRL HFAKNSTTI NTAGATVRQ LVSLVFERV 
      VAEDELLSK NDTAPVQKQ INMEDFKVP SGTPPKGLP 
      PCAADAYLL FQDLVQLVN ADQPYWLIG MTEMTRTFG 
      LELLESVLT QFSVVFYKN AEFSFLLKE RVCALVIKL 
      FSPNIKYRS TMPSNVQQA TPFEKPYFP ISMRLLRVV 
      SILIQKYHS LLVTECEIF LSLIVKFLD PDKPTWQRS 
      LALEVLHKM TIQPELLNS FCKCYDLNK HTTSIFQDI 
      VNSLGAYVQ SLFVNAQLQ MTTMTITQG QPPVFLGGL 
      PAGPGVSPQ PGFLMRGVW LPIVTTFPT GQTKSIYLE 
      MLDKIEPPT IPDGYGISI AYASLLEII RSLQITIGP 
      QQQGEAEPP PPPEPTEDE RKLHTQLII SSWCGLLAA 
      LTPLIDAST DESVTENIL KALQTYASL SGELGLDTP 
      RDAFITAIC KSSLPPHYA LTVLNTVSS GIRTGHRDG 
      QELHSIANY GEADYRQQV VAVGTPLPT SSVPAGTQQ 
      GPVMLTSKN LQAMRALLS LAHCHGSIL GTSWHLVLT 
      TLQHLVWIL GLKPSTGGS LKAGRFTTD SNAVITTSV 
      MADLPVLSQ MLSRLFETS QYLDDVALH HLINALCKL 
      SQEAMELAY SNREPSLFA VAKLLETGL VNMPRIEVL 
      WRPLTNHLL EVCRHPHIR MREWGVEAI TYLVKSALH 
      YKHTVPLRD NQKLQTLLL GPLFELSSV PHGDVRQRQ 
      LECVLEILH GAGETLSHG WPLVLGIIG AVSDQHGEN 
      LIRIAFQCL QLVITDFLP VMPWRCLPL CVDTVAKFG 
      SQTQELNIS LTAVGLMWN ISDYFHQNQ GKLSQTLTE 
      DTTVLPDFP GTLNMPSFD KLWMCLYAR LGELCVDSR 
      PAVRKSAGQ TLFSTISAH GGLLKQSTW QAILWQVLF 
      PLLDKVRNL SNSASSEKV DAGGNILIH HTRNTAQKQ 
      WAETQVLTL SGVARVFNT KRQLLQALG DFPRAWSIL 
      LEFIENAAL SKNNEVSIA ALKSFQEIL FVSKTQPSD 
      NKALIEDRE IWAIAWRVW VKIGTEITI PALESEQTD 
      DFYFPSQTF LTALVQIFP SVFQHIKSN FNLDDLKQL 
      GTVLTNAVS VPVYGESTP YMISTSSDV SLTPLHDGV 
      LHTMELLQK EALARNNSK MIPELFKRL LIFARFTCT 
      PPNSTRLDN RHAKVSDWI TMNYIPFGE KAMTMTVKL 
      YEKTAENPE VIGGNILHG IIAALHKPL ALKYNCFSN 
      SIWKLAANS LITVLKTGL KVARANGNQ FAPMWPELA 
      ATLNDFLFP SMAAPADKG LDEMVSDEA TDCQLIELL 
      RTEVLPYSS QIPKDFIMQ VVVLLNKGS IHSATNVKT 
      DSDSELTLR EEFAKTCFE TLLQFSLID GDNRALVVN 
      GDDLAGQLA VTSLLQRFQ EVLEKYIED EKLSGKCPL 
      PRYRLSEIS FVLKAVTTL IISMKKAPV KEDSTGWEH 
      LIKLYPFLV ECTTTTSTQ VSRPLREAL IQFCDLLQP 
      PVTKKTNGE KY
    CDS sequence
    • >TC004594 RA
      atggcgttt gagaatggt gtgcacaat gtggaagta 
      tcagcggca tttatcgag tcgttacaa accgatttc 
      aagaacctg gcctcggaa tctaagaag aagtatccg 
      gttataaaa gagtcatgt gaggaggcg attgttaaa 
      ctcaaagca gcggtttca acccctcag acttccatt 
      tactacgtt atcaaccaa attatttac cccttgtca 
      cagggttgc gaaactaaa gatgttaaa ttgattaaa 
      ggttgctta caaatcata caaaaatta ataacacac 
      cagctgatt gatcaaaaa ggcgcgctt tacatcact 
      gacacttta tggactttg atggaatca gggattgaa 
      gaagttaaa attctccag tcggtcact ttgctactg 
      acgacgaac acggtagta catggggac acgctagca 
      aggaccctg gttttgtgt ttccgcttg cattttgcc 
      aaaaattcg acgacgatt aacacagct ggggccaca 
      gttagacag ttggtgtct ctcgtgttc gaacgagtg 
      gttgcagag gacgaactc ttatcaaaa aatgatact 
      gctccagtg cagaaacaa atcaacatg gaagacttc 
      aaagtacca agcggtact cccccgaaa gggctgccc 
      ccatgtgcc gccgatgcg tatttgtta tttcaggac 
      ttggtccaa ttagtaaac gccgatcaa ccctactgg 
      ctgatcggc atgactgaa atgacgcga actttcggc 
      ttggagttg cttgagtcg gttttgacc caattttcg 
      gtcgttttc tacaaaaac gccgagttc agttttctc 
      ctgaaggag cgggtctgt gctcttgtc atcaagcta 
      ttttcgccc aatattaag tacaggagc accatgccg 
      tcgaacgtg cagcaagca acccctttt gagaagccc 
      tattttccg ataagcatg agactgtta cgggttgtg 
      tctattttg atccagaag taccacagt cttctggtg 
      actgaatgc gaaatattc ttatctctg attgtgaaa 
      tttttggac cctgataag cccacgtgg cagcgatca 
      ctcgccttg gaagtgttg cacaaaatg accattcaa 
      cccgaactt ttgaattcg ttttgtaaa tgttatgat 
      ctcaacaaa cacaccact agtattttt caagatatt 
      gtcaattcg ttaggggct tacgtccag tcactgttt 
      gtaaatgca cagttacaa atgacgacg atgactata 
      acacaaggc caacctccg gtgtttctg gggggttta 
      ccggccggt ccgggggtt tccccccag cctggtttt 
      ctgatgcgc ggagtttgg ctgccaatt gtgaccact 
      tttccaaca ggacaaact aaatctatt tatttggaa 
      atgttggat aagattgag ccgcctaca atccccgac 
      ggttatggc atttcgatc gcttatgcc agtcttttg 
      gagataatt cgttcgtta caaatcaca atcgggcct 
      cagcaacaa ggcgaagcc gagccgccg ccaccacct 
      gagcctaca gaagacgag cgcaaactc cacacccag 
      ttgataatt tcaagttgg tgtgggtta cttgcagct 
      ttaacgcca ctgattgat gcaagtacc gacgaatca 
      gtcaccgaa aacatccta aaagcgttg caaacttac 
      gcttcgctg agcggcgaa ttggggttg gacactcca 
      agggatgcg tttattacc gcaatttgc aaatcgtcc 
      cttccgcca cattacgcc cttactgtc ttaaacacc 
      gtgtcgagt ggcattcgg acagggcac cgagacggc 
      caggaattg cactcgatt gcaaattac ggcgaagct 
      gattataga caacaagtc gtcgccgtc gggacgccc 
      ctcccgacg tcgtcagtg cctgctggg acgcagcaa 
      ggcccagtt atgctcact tcgaaaaat ttacaggca 
      atgcgagcg ttacttagc ttggctcat tgtcacggg 
      agcatctta ggcacttcc tggcattta gttttgaca 
      actttgcaa catcttgtt tggattttg ggcctaaag 
      ccctcaacc ggcggtagt ttgaaagca ggtcgcttc 
      acaaccgac tcaaacgcg gttataacc acttcggta 
      atggccgat ttgccggtt ttgtcgcaa atgttgagc 
      cggttgttt gaaacgagc cagtacctc gacgacgta 
      gccttgcac caccttatc aacgctctc tgtaaacta 
      tcacaagag gccatggaa ctagcctac tcgaatcgt 
      gagccgtcg ctgtttgcc gttgctaag ttactcgaa 
      accggtttg gttaacatg ccacgaatt gaggttctg 
      tggcggcct ttaaccaat catttgttg gaagtttgt 
      cggcatcct catatcaga atgagagaa tggggggtt 
      gaggctata acgtattta gtcaagtcg gccttgcat 
      tataaacat acggttccg ttgagagat aaccaaaaa 
      ttgcaaact ttgcttttg ggaccgctc tttgaactg 
      tcgtcagtc ccacatggg gatgtaagg cagaggcag 
      ctagagtgt gtccttgag attttgcac ggagcggga 
      gaaacgctc tcgcacgga tggccactc gttttgggc 
      ataattggc gccgtcagc gaccaacac ggtgaaaat 
      ttaatacgg atcgcgttt caatgcctc cagctggtt 
      ataaccgac ttcctccct gtgatgccg tggcgctgt 
      ttgccacta tgcgtcgac actgttgcc aaattcggc 
      tcccaaacc caggaatta aacatttct ttaaccgca 
      gtcggactt atgtggaac atttccgac tactttcac 
      caaaaccag ggcaagttg agccagacg ttgaccgag 
      gacactacc gtcctccct gactttcca ggcactttg 
      aatatgcca agtttcgac aaactctgg atgtgtcta 
      tacgcacgt ctcggcgaa ctttgcgtc gattcgcga 
      ccggctgtg cgcaaatca gccggtcag accctattt 
      tcaacaatc tcagcacac ggcgggctt ttgaaacaa 
      tcaacctgg caggcgatt ctctggcag gtgttgttc 
      cctctcctc gataaggtc cgaaactta tcgaattca 
      gcaagcagt gagaaagtt gacgctggg ggcaacata 
      ctgatacac catacgcgc aacacggcg cagaaacaa 
      tgggcggag acgcaagtt ttaaccctg tcgggtgtg 
      gcacgggtg tttaacacg aagcgacag ttgttgcaa 
      gccctgggt gatttcccg cgtgcctgg tcgatcctc 
      ctggaattc atcgaaaat gcggccttg agtaaaaat 
      aacgaggtt tcaatcgcc gcacttaag tcgttccag 
      gagattctc ttcgtgagc aagacgcag ccgagtgat 
      aataaggcc ctgattgag gatcgggag atttgggcc 
      attgcgtgg agggtttgg gtcaaaatc gggacggag 
      ataacgatc ccggccctt gagagcgaa caaaccgat 
      gatttttac ttcccgagt cagacgttt ctgacggct 
      ttggtccag atattcccc agcgtgttc cagcatatt 
      aagagcaac ttcaatttg gacgacttg aagcagttg 
      gggactgtg ctgactaac gcggtcagt gtcccggtt 
      tatggcgag tcgactcct tacatgatc tcaacttcg 
      tcagacgtg agtctgacg ccgttacac gacggcgtg 
      ttgcacacc atggagttg ctgcaaaag gaggctctc 
      gcgaggaat aacagcaaa atgatcccg gagttgttc 
      aagcggttg ctcattttt gcccgattc acctgcacg 
      ccgccgaat tcgaccaga ctggacaac cgacatgcg 
      aaggtttcc gattggatc acaatgaac tatattcca 
      ttcggcgaa aaggccatg actatgacg gtcaaactg 
      tacgaaaaa acggccgaa aatccggaa gtgatcggc 
      gggaatatc ctacatggg ataatcgca gccttgcat 
      aaacctcta gctttaaaa tacaactgc ttctctaac 
      agtatttgg aaactggcg gctaatagc ctaatcact 
      gtgttaaaa accggcctg aaggtggcg cgcgccaac 
      ggcaaccag ttcgcccca atgtggccc gaactggcc 
      gcgactttg aacgatttc ctgttcccc agcatggcg 
      gcgccggct gataaaggc ttggacgag atggtgtcc 
      gacgaagcg accgattgc cagctcatc gagctgctg 
      aggaccgaa gtcttgccc tattcgagt caaataccg 
      aaagatttt atcatgcaa gttgtggtt ttgctgaac 
      aagggttcg attcattcc gcgacgaat gtcaagact 
      gacagcgat tcggagttg acgctgcgg gaggagttc 
      gccaagacg tgtttcgag actttgctg cagttttcg 
      ttaattgat ggcgataac agggcgctg gtggtcaac 
      ggtgatgac ttggcggga cagcttgcg gtcacgtcg 
      ctcttgcag cggtttcag gaggttttg gagaagtac 
      atcgaggac gagaagctg agcgggaag tgtcctttg 
      ccgaggtac aggctttcg gagatctcg ttcgtgttg 
      aaagctgtg accaccctg atcatatca atgaaaaag 
      gcgcccgtt aaagaagat agtacagga tgggaacat 
      ttgataaag ttgtacccg tttttagtc gaatgtaca 
      acgaccaca tccacgcaa gtttcaagg ccgttgcgg 
      gaagctctt atacaattc tgtgattta ttgcaacca 
      cctgtgact aagaaaaca aacggtgaa aaatactga
    mRNA sequence
    • >TC004594 RA
      cggctagtc ggtgttgca ggtcgaatg ttttgtaat 
      tagtcaaat aattataaa taatgtcgc aatggcaat 
      aagtgaaat taaataaaa ctagagtga attgctggt 
      gaaaaccat gtggtgaaa ctcacccgg cgaatgaac 
      aattcgaag tgacagtga caaatgaca agtgccagc 
      tcagcttaa tgttttcaa aggattttc atttattcg 
      actaatttt cgaaaatta tggcgtttg agaatggtg 
      tgcacaatg tggaagtat cagcggcat ttatcgagt 
      cgttacaaa ccgatttca agaacctgg cctcggaat 
      ctaagaaga agtatccgg ttataaaag agtcatgtg 
      aggaggcga ttgttaaac tcaaagcag cggtttcaa 
      cccctcaga cttccattt actacgtta tcaaccaaa 
      ttatttacc ccttgtcac agggttgcg aaactaaag 
      atgttaaat tgattaaag gttgcttac aaatcatac 
      aaaaattaa taacacacc agctgattg atcaaaaag 
      gcgcgcttt acatcactg acactttat ggactttga 
      tggaatcag ggattgaag aagttaaaa ttctccagt 
      cggtcactt tgctactga cgacgaaca cggtagtac 
      atggggaca cgctagcaa ggaccctgg ttttgtgtt 
      tccgcttgc attttgcca aaaattcga cgacgatta 
      acacagctg gggccacag ttagacagt tggtgtctc 
      tcgtgttcg aacgagtgg ttgcagagg acgaactct 
      tatcaaaaa atgatactg ctccagtgc agaaacaaa 
      tcaacatgg aagacttca aagtaccaa gcggtactc 
      ccccgaaag ggctgcccc catgtgccg ccgatgcgt 
      atttgttat ttcaggact tggtccaat tagtaaacg 
      ccgatcaac cctactggc tgatcggca tgactgaaa 
      tgacgcgaa ctttcggct tggagttgc ttgagtcgg 
      ttttgaccc aattttcgg tcgttttct acaaaaacg 
      ccgagttca gttttctcc tgaaggagc gggtctgtg 
      ctcttgtca tcaagctat tttcgccca atattaagt 
      acaggagca ccatgccgt cgaacgtgc agcaagcaa 
      ccccttttg agaagccct attttccga taagcatga 
      gactgttac gggttgtgt ctattttga tccagaagt 
      accacagtc ttctggtga ctgaatgcg aaatattct 
      tatctctga ttgtgaaat ttttggacc ctgataagc 
      ccacgtggc agcgatcac tcgccttgg aagtgttgc 
      acaaaatga ccattcaac ccgaacttt tgaattcgt 
      tttgtaaat gttatgatc tcaacaaac acaccacta 
      gtatttttc aagatattg tcaattcgt taggggctt 
      acgtccagt cactgtttg taaatgcac agttacaaa 
      tgacgacga tgactataa cacaaggcc aacctccgg 
      tgtttctgg ggggtttac cggccggtc cgggggttt 
      ccccccagc ctggttttc tgatgcgcg gagtttggc 
      tgccaattg tgaccactt ttccaacag gacaaacta 
      aatctattt atttggaaa tgttggata agattgagc 
      cgcctacaa tccccgacg gttatggca tttcgatcg 
      cttatgcca gtcttttgg agataattc gttcgttac 
      aaatcacaa tcgggcctc agcaacaag gcgaagccg 
      agccgccgc caccacctg agcctacag aagacgagc 
      gcaaactcc acacccagt tgataattt caagttggt 
      gtgggttac ttgcagctt taacgccac tgattgatg 
      caagtaccg acgaatcag tcaccgaaa acatcctaa 
      aagcgttgc aaacttacg cttcgctga gcggcgaat 
      tggggttgg acactccaa gggatgcgt ttattaccg 
      caatttgca aatcgtccc ttccgccac attacgccc 
      ttactgtct taaacaccg tgtcgagtg gcattcgga 
      cagggcacc gagacggcc aggaattgc actcgattg 
      caaattacg gcgaagctg attatagac aacaagtcg 
      tcgccgtcg ggacgcccc tcccgacgt cgtcagtgc 
      ctgctggga cgcagcaag gcccagtta tgctcactt 
      cgaaaaatt tacaggcaa tgcgagcgt tacttagct 
      tggctcatt gtcacggga gcatcttag gcacttcct 
      ggcatttag ttttgacaa ctttgcaac atcttgttt 
      ggattttgg gcctaaagc cctcaaccg gcggtagtt 
      tgaaagcag gtcgcttca caaccgact caaacgcgg 
      ttataacca cttcggtaa tggccgatt tgccggttt 
      tgtcgcaaa tgttgagcc ggttgtttg aaacgagcc 
      agtacctcg acgacgtag ccttgcacc accttatca 
      acgctctct gtaaactat cacaagagg ccatggaac 
      tagcctact cgaatcgtg agccgtcgc tgtttgccg 
      ttgctaagt tactcgaaa ccggtttgg ttaacatgc 
      cacgaattg aggttctgt ggcggcctt taaccaatc 
      atttgttgg aagtttgtc ggcatcctc atatcagaa 
      tgagagaat ggggggttg aggctataa cgtatttag 
      tcaagtcgg ccttgcatt ataaacata cggttccgt 
      tgagagata accaaaaat tgcaaactt tgcttttgg 
      gaccgctct ttgaactgt cgtcagtcc cacatgggg 
      atgtaaggc agaggcagc tagagtgtg tccttgaga 
      ttttgcacg gagcgggag aaacgctct cgcacggat 
      ggccactcg ttttgggca taattggcg ccgtcagcg 
      accaacacg gtgaaaatt taatacgga tcgcgtttc 
      aatgcctcc agctggtta taaccgact tcctccctg 
      tgatgccgt ggcgctgtt tgccactat gcgtcgaca 
      ctgttgcca aattcggct cccaaaccc aggaattaa 
      acatttctt taaccgcag tcggactta tgtggaaca 
      tttccgact actttcacc aaaaccagg gcaagttga 
      gccagacgt tgaccgagg acactaccg tcctccctg 
      actttccag gcactttga atatgccaa gtttcgaca 
      aactctgga tgtgtctat acgcacgtc tcggcgaac 
      tttgcgtcg attcgcgac cggctgtgc gcaaatcag 
      ccggtcaga ccctatttt caacaatct cagcacacg 
      gcgggcttt tgaaacaat caacctggc aggcgattc 
      tctggcagg tgttgttcc ctctcctcg ataaggtcc 
      gaaacttat cgaattcag caagcagtg agaaagttg 
      acgctgggg gcaacatac tgatacacc atacgcgca 
      acacggcgc agaaacaat gggcggaga cgcaagttt 
      taaccctgt cgggtgtgg cacgggtgt ttaacacga 
      agcgacagt tgttgcaag ccctgggtg atttcccgc 
      gtgcctggt cgatcctcc tggaattca tcgaaaatg 
      cggccttga gtaaaaata acgaggttt caatcgccg 
      cacttaagt cgttccagg agattctct tcgtgagca 
      agacgcagc cgagtgata ataaggccc tgattgagg 
      atcgggaga tttgggcca ttgcgtgga gggtttggg 
      tcaaaatcg ggacggaga taacgatcc cggcccttg 
      agagcgaac aaaccgatg atttttact tcccgagtc 
      agacgtttc tgacggctt tggtccaga tattcccca 
      gcgtgttcc agcatatta agagcaact tcaatttgg 
      acgacttga agcagttgg ggactgtgc tgactaacg 
      cggtcagtg tcccggttt atggcgagt cgactcctt 
      acatgatct caacttcgt cagacgtga gtctgacgc 
      cgttacacg acggcgtgt tgcacacca tggagttgc 
      tgcaaaagg aggctctcg cgaggaata acagcaaaa 
      tgatcccgg agttgttca agcggttgc tcatttttg 
      cccgattca cctgcacgc cgccgaatt cgaccagac 
      tggacaacc gacatgcga aggtttccg attggatca 
      caatgaact atattccat tcggcgaaa aggccatga 
      ctatgacgg tcaaactgt acgaaaaaa cggccgaaa 
      atccggaag tgatcggcg ggaatatcc tacatggga 
      taatcgcag ccttgcata aacctctag ctttaaaat 
      acaactgct tctctaaca gtatttgga aactggcgg 
      ctaatagcc taatcactg tgttaaaaa ccggcctga 
      aggtggcgc gcgccaacg gcaaccagt tcgccccaa 
      tgtggcccg aactggccg cgactttga acgatttcc 
      tgttcccca gcatggcgg cgccggctg ataaaggct 
      tggacgaga tggtgtccg acgaagcga ccgattgcc 
      agctcatcg agctgctga ggaccgaag tcttgccct 
      attcgagtc aaataccga aagatttta tcatgcaag 
      ttgtggttt tgctgaaca agggttcga ttcattccg 
      cgacgaatg tcaagactg acagcgatt cggagttga 
      cgctgcggg aggagttcg ccaagacgt gtttcgaga 
      ctttgctgc agttttcgt taattgatg gcgataaca 
      gggcgctgg tggtcaacg gtgatgact tggcgggac 
      agcttgcgg tcacgtcgc tcttgcagc ggtttcagg 
      aggttttgg agaagtaca tcgaggacg agaagctga 
      gcgggaagt gtcctttgc cgaggtaca ggctttcgg 
      agatctcgt tcgtgttga aagctgtga ccaccctga 
      tcatatcaa tgaaaaagg cgcccgtta aagaagata 
      gtacaggat gggaacatt tgataaagt tgtacccgt 
      ttttagtcg aatgtacaa cgaccacat ccacgcaag 
      tttcaaggc cgttgcggg aagctctta tacaattct 
      gtgatttat tgcaaccac ctgtgacta agaaaacaa 
      acggtgaaa aatactgaa tagtgtttg tacctgccc 
      caatatcaa tcgtgtctg atggttgat ttgaatttt 
      cttttgttc aacacacac ttccagcgt tttgccatg 
      ttttagttt tgattcatg ttatagttt ataaatttg 
      taaatttta gcgtttcaa aataaaata ttgaaaatt 
      gc
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