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Gene information
General information
TC004592 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7041023..7054290
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004592
    iBeetle screen iB_03794

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004592 RA
      MQVLTVFIS IILIGISEE QSKPMKPTV PTPSYDQKP 
      SGEYWPGSW DNNSTTVRQ NLANWLLNG SWNNPERIP 
      EKSHEYEGY DGWYNNIAH PDLGAIDRP LLRRWPTAY 
      EDGSYLPAG SNRPNPLEL SEKLLKGET GTKSRAGKN 
      ALIVFFGQQ VVEEILDAQ RPACPPEYF NIEVPQNHT 
      YLEKPGHSQ MPFLRTRYD QRTGYSPNN PRQQLNEIT 
      PYLDGGLFY GTTKQWADQ LRTYENGTI DPDGCLASS 
      HDGLFPAYN TQRLPLANP PPPIYHSMF IKSHETAKV 
      SRFFKLGNP RGNENSFLL TFGIMWFRW HNYLAKRIR 
      ILRPEWPSE KVFNEARKW VIATQQKIV VYDWLPEWI 
      FEDLPDYGG YDPGVDPQI DQFFQSAAF RFGHTLVVP 
      GVYLRDYAR NGCKHKFDT WGQNTVRTC NSFWRPYEP 
      IVKPVDEGK SEVIDIDRL LMGMAVQLC EEEDHKIVE 
      DLRGNVFGP LEFPRRDLM ALNIQRGRD HGIPDYNTV 
      RRAYGLKKQ TFGDFGHLP MKIKEEFEK LYSSDEDVD 
      LWIGGILET KDHKPGELF RTIIKDQFR RIRDGDRFW 
      FENKENGLF SNDEVERIK QITLYDVIM AVTRMDPDD 
      LPKNVFNVP TDTDVQLIS KCNLTKSSC TSKDSTNFN 
      CHHLEPLTT SVLNESCVQ GLSYDYFSN SEVSFIVTF 
      ISIGTFIIG SIGVIYVLI QVKEKQILA AKIVANQST 
      RNTFKNEKN IHYPLFVVD EWLGPKDPS RRVVLVLKD 
      DKKIVEVMS QTGELLRAL DFTNPKKKV EVYEIKDAP 
      YVIIKCTHD YDLILRFET DYLRSRFLT TFDPFLTNS 
      GLARENISS FTWKAALKK ITTKTDRQL LLERFYRVV 
      FAQAFKIKH SQEELLHVD ETLEKEIVD TELTITEFA 
      EALSMRAQD EFVKRMFAL VDKDKSGFI SFREFVDLL 
      IIFANGTEE NKAKLLFDM YDVNAVGYL TEENFIAMI 
      K
    CDS sequence
    • >TC004592 RA
      atgcaagtt ttaactgtg tttatatcc ataatttta 
      attggaata agcgaggag cagtccaaa ccgatgaag 
      ccaacagtg ccaaccccc agttatgac caaaaaccg 
      tcaggagaa tattggcct ggctcttgg gataataat 
      agcacaacc gttcggcaa aacctggca aactggtta 
      ctgaacggc tcctggaac aacccggag cgaatcccg 
      gaaaaatcg cacgaatac gagggatac gacgggtgg 
      tacaacaac atagcccac ccggattta ggcgcaatt 
      gatcggcca cttttgcgc cgttggccc acggcctac 
      gaagacggt agttacctg ccagctggg tcaaaccga 
      ccaaatccc ttggagcta agcgaaaaa ttattgaag 
      ggggaaacg ggaactaaa tcaagggcg gggaaaaat 
      gccttgatt gtttttttc gggcaacaa gtcgttgag 
      gaaattttg gatgctcaa aggccggcc tgccctccg 
      gaatatttt aatattgag gtgcctcaa aaccacacg 
      tatttggag aagccggga cattcgcaa atgccattt 
      ttgaggact cggtacgac caaaggacg ggctacagc 
      cccaataat cccagacaa caattgaac gaaatcacc 
      ccttacctg gacgggggt ttgttctac ggcaccaca 
      aaacaatgg gccgaccaa ttacgcacc tacgaaaac 
      ggaaccatc gacccagat gggtgcctc gcatcgtcc 
      catgatggt ctattccca gcttacaac acccagcgt 
      cttcccttg gccaacccc ccgcccccg atctaccac 
      tccatgttt atcaaatcg cacgaaacg gcaaaagtg 
      tcgcgattt ttcaagttg ggaaacccg cgaggaaac 
      gaaaactca tttctccta acttttgga ataatgtgg 
      ttccggtgg cacaattac ctcgcgaaa aggatccgg 
      attttgcga cctgagtgg cccagtgag aaggttttt 
      aacgaggcc cggaaatgg gtgattgca acccagcaa 
      aaaatcgtg gtttacgac tggttgccg gagtggatt 
      ttcgaggac ttgccggat tatggggga tatgatccg 
      ggggttgac ccccaaatt gaccagttt ttccaaagt 
      gcggcgttc cggttcgga cacactttg gtcgttccg 
      ggggtgtat ttgcgggat tacgcccgg aacgggtgc 
      aaacacaag tttgacact tgggggcaa aacacggta 
      cggacctgt aactccttc tggaggccc tatgaaccc 
      attgtcaag ccggttgat gaaggaaag agcgaagtt 
      attgacatt gaccgactt ttgatgggg atggccgtg 
      caattgtgc gaagaagaa gaccacaaa atcgttgaa 
      gacttgcgg ggaaatgtg ttcggccct ttggaattt 
      cctcgaagg gacctgatg gccctaaat atacaaagg 
      gggcgcgat cacggaatc cctgattac aacacagtt 
      cggcgagct tatggcttg aaaaaacag acattcggc 
      gattttggt catctccca atgaagatc aaagaagaa 
      ttcgagaag ttgtacagt tcagacgaa gatgttgat 
      ctctggatc ggtggcatc cttgagacg aaggaccac 
      aaacctggc gagcttttc cggactatt atcaaggac 
      cagttccgg aggatccgg gacggggac cgtttttgg 
      ttcgaaaac aaagaaaat ggcctattc agcaatgac 
      gaagttgaa agaattaaa caaattaca ctgtacgat 
      gttataatg gcagtcaca aggatggac ccggacgac 
      ttgcccaag aacgtattt aatgtccct actgacact 
      gatgttcaa ttgatttcg aaatgtaac ttgacaaag 
      tcgtcgtgt acgagtaaa gacagtaca aacttcaac 
      tgccaccac ctggagcca ctcacaacg agtgtgtta 
      aatgaatcc tgtgtgcaa ggattgtcc tacgattat 
      ttcagcaat agcgaagtt tcatttatt gtgacgttt 
      attagcatt gggacgttt atcatagga tcgatcgga 
      gtcatctac gtgttaata caagtgaag gagaaacaa 
      atactggcc gcaaaaatt gtagcgaat caatccact 
      cgaaatacg ttcaaaaac gagaaaaac attcattat 
      cctttgttt gttgtggat gaatggctt ggcccgaaa 
      gatccttca aggcgtgtg gttttggtc ctaaaagac 
      gacaaaaaa atcgtcgag gtgatgtcg caaactggc 
      gaattactc cgcgccctt gactttaca aacccaaag 
      aaaaaagtc gaagtttac gaaatcaag gacgcccct 
      tacgtcatc attaaatgc acgcatgat tacgaccta 
      attttgcga tttgagacg gattattta aggagcaga 
      ttcctcaca acgtttgat cctttctta acaaacagt 
      gggttagct cgcgaaaac atctcgagc tttacctgg 
      aaagcggct ttgaagaaa attacaaca aaaactgac 
      cgacaattg ctcctggaa cggttctat cgagtggtt 
      ttcgcccaa gctttcaaa ataaagcac tcgcaggag 
      gagttgctg cacgttgat gagactctg gagaaggag 
      attgttgat actgagtta accattact gagtttgcg 
      gaagctttg agtatgagg gcacaagac gagttcgtt 
      aagcgaatg ttcgcgctt gttgataag gacaagagc 
      gggtttatc tcatttcgg gagtttgtc gacttgttg 
      attattttt gccaacggg acagaggag aacaaagcc 
      aagttgttg ttcgatatg tacgatgtt aacgccgtg 
      ggataccta acagaggag aatttcatc gcaatgatt 
      aagtaa
    mRNA sequence
    • >TC004592 RA
      tccatacag attaaaata ccatcagtc aaatgtgtg 
      ctttcttgc aacaatgca agttttaac tgtgtttat 
      atccataat tttaattgg aataagcga ggagcagtc 
      caaaccgat gaagccaac agtgccaac ccccagtta 
      tgaccaaaa accgtcagg agaatattg gcctggctc 
      ttgggataa taatagcac aaccgttcg gcaaaacct 
      ggcaaactg gttactgaa cggctcctg gaacaaccc 
      ggagcgaat cccggaaaa atcgcacga atacgaggg 
      atacgacgg gtggtacaa caacatagc ccacccgga 
      tttaggcgc aattgatcg gccactttt gcgccgttg 
      gcccacggc ctacgaaga cggtagtta cctgccagc 
      tgggtcaaa ccgaccaaa tcccttgga gctaagcga 
      aaaattatt gaaggggga aacgggaac taaatcaag 
      ggcggggaa aaatgcctt gattgtttt tttcgggca 
      acaagtcgt tgaggaaat tttggatgc tcaaaggcc 
      ggcctgccc tccggaata ttttaatat tgaggtgcc 
      tcaaaacca cacgtattt ggagaagcc gggacattc 
      gcaaatgcc atttttgag gactcggta cgaccaaag 
      gacgggcta cagccccaa taatcccag acaacaatt 
      gaacgaaat cacccctta cctggacgg gggtttgtt 
      ctacggcac cacaaaaca atgggccga ccaattacg 
      cacctacga aaacggaac catcgaccc agatgggtg 
      cctcgcatc gtcccatga tggtctatt cccagctta 
      caacaccca gcgtcttcc cttggccaa ccccccgcc 
      cccgatcta ccactccat gtttatcaa atcgcacga 
      aacggcaaa agtgtcgcg atttttcaa gttgggaaa 
      cccgcgagg aaacgaaaa ctcatttct cctaacttt 
      tggaataat gtggttccg gtggcacaa ttacctcgc 
      gaaaaggat ccggatttt gcgacctga gtggcccag 
      tgagaaggt ttttaacga ggcccggaa atgggtgat 
      tgcaaccca gcaaaaaat cgtggttta cgactggtt 
      gccggagtg gattttcga ggacttgcc ggattatgg 
      gggatatga tccgggggt tgaccccca aattgacca 
      gtttttcca aagtgcggc gttccggtt cggacacac 
      tttggtcgt tccgggggt gtatttgcg ggattacgc 
      ccggaacgg gtgcaaaca caagtttga cacttgggg 
      gcaaaacac ggtacggac ctgtaactc cttctggag 
      gccctatga acccattgt caagccggt tgatgaagg 
      aaagagcga agttattga cattgaccg acttttgat 
      ggggatggc cgtgcaatt gtgcgaaga agaagacca 
      caaaatcgt tgaagactt gcggggaaa tgtgttcgg 
      ccctttgga atttcctcg aagggacct gatggccct 
      aaatataca aagggggcg cgatcacgg aatccctga 
      ttacaacac agttcggcg agcttatgg cttgaaaaa 
      acagacatt cggcgattt tggtcatct cccaatgaa 
      gatcaaaga agaattcga gaagttgta cagttcaga 
      cgaagatgt tgatctctg gatcggtgg catccttga 
      gacgaagga ccacaaacc tggcgagct tttccggac 
      tattatcaa ggaccagtt ccggaggat ccgggacgg 
      ggaccgttt ttggttcga aaacaaaga aaatggcct 
      attcagcaa tgacgaagt tgaaagaat taaacaaat 
      tacactgta cgatgttat aatggcagt cacaaggat 
      ggacccgga cgacttgcc caagaacgt atttaatgt 
      ccctactga cactgatgt tcaattgat ttcgaaatg 
      taacttgac aaagtcgtc gtgtacgag taaagacag 
      tacaaactt caactgcca ccacctgga gccactcac 
      aacgagtgt gttaaatga atcctgtgt gcaaggatt 
      gtcctacga ttatttcag caatagcga agtttcatt 
      tattgtgac gtttattag cattgggac gtttatcat 
      aggatcgat cggagtcat ctacgtgtt aatacaagt 
      gaaggagaa acaaatact ggccgcaaa aattgtagc 
      gaatcaatc cactcgaaa tacgttcaa aaacgagaa 
      aaacattca ttatccttt gtttgttgt ggatgaatg 
      gcttggccc gaaagatcc ttcaaggcg tgtggtttt 
      ggtcctaaa agacgacaa aaaaatcgt cgaggtgat 
      gtcgcaaac tggcgaatt actccgcgc ccttgactt 
      tacaaaccc aaagaaaaa agtcgaagt ttacgaaat 
      caaggacgc cccttacgt catcattaa atgcacgca 
      tgattacga cctaatttt gcgatttga gacggatta 
      tttaaggag cagattcct cacaacgtt tgatccttt 
      cttaacaaa cagtgggtt agctcgcga aaacatctc 
      gagctttac ctggaaagc ggctttgaa gaaaattac 
      aacaaaaac tgaccgaca attgctcct ggaacggtt 
      ctatcgagt ggttttcgc ccaagcttt caaaataaa 
      gcactcgca ggaggagtt gctgcacgt tgatgagac 
      tctggagaa ggagattgt tgatactga gttaaccat 
      tactgagtt tgcggaagc tttgagtat gagggcaca 
      agacgagtt cgttaagcg aatgttcgc gcttgttga 
      taaggacaa gagcgggtt tatctcatt tcgggagtt 
      tgtcgactt gttgattat ttttgccaa cgggacaga 
      ggagaacaa agccaagtt gttgttcga tatgtacga 
      tgttaacgc cgtgggata cctaacaga ggagaattt 
      catcgcaat gattaagta atttttttg aatcttgtg 
      aaatcagcc accttagtg caaaagaat tttcttaat 
      gactaatga ctagagact agattttta gaaagttaa 
      aaacattat ttatttcta ctgcagtaa aattaaaaa 
      cacattttt tattgcaat aaactgcat tataaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY