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Gene information
General information
TC034531 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7089942..7145291
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034531
    iBeetle screen iB_03793

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    labium partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    prothorax mostlynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    Pupal morphology
    wings shortened - Number of animals showing the phenotype: 4

    oblique dorsal thoracic muscles shortened - Number of animals showing the phenotype: 4
    iBeetle screen iB_07331

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034531 RA
      MTRRNMEDM PLNSSPSLE ADGSMMSPP PPQASPADR 
      SPHQDPPES PAMLATMTP VNVIELKKE MEEESAMAR 
      APLYAQVKT EVISYDKDE LYAQPLPDK NDTPIDLIY 
      EDGNKTVIY TTAFDQKSL EIYSADNGG EITINNLSA 
      HQIHSEGLI DGNSGAIAG GEPTGSPPG TVSVVLQGN 
      QGLLQYPTQ SQVPTVLVL SEMTEDEGV PVIGLRKRR 
      DNLESEGNS LHQSSSGGE QLPVVVQRG MAELPEGAA 
      PPEDQHPLT AATTAMLNI SGGAEDQST AMGLIYEYY 
      KFQPISGES KDKLADIWP ASSQAGGIL TSQLKATNG 
      LVSGAGLEL PTPPQLSSQ DWQGLFINQ HVGYPQAAA 
      SAQNFQLVK REPEDLSHH RKLDCSSPS SVSLDGAII 
      NTKQQRHKV VLPAMYATQ IFAAMTNNG HGGSGTPSP 
      TPYSDHVTG YTQSGAGYA AASRTATSA AAFVSDPYY 
      REYFTVASG GATPEPAYS GQLRQSQAY SEEVGGGTT 
      ASFVERYVR QPYHSKGVI AAATAAGLT VDLPSPDSG 
      IGADAITPR DQNTLQQNY EYPEICPNP NILDPLTIS 
      QRNSSSSQS PGSQSTSRS RPWHDFGRQ NDADKIQIP 
      KIFSPVGFK YQLETPIST SQRREDDRI TYINKGQFY 
      GITLDYIPD PDKQLKSQT VKSIVMLMF REEKSPEDE 
      IKAWQFWHG RQHSVKQRI LDADTKNSV GLVGCIEEV 
      AHNAIAIYW NPLESPAKI NIAVQCLST DFSSQKGVK 
      GLPLHLQID TYEDPRDTN VYHRGYCQI KVFCDKGAE 
      RKTRDEERR AAKRKMTAT GRKKLDELY HPMCERSEF 
      YSMSDLAKP PVLFSPAED IDKLTSMEL QGFYPHDTD 
      SSLTGPVDH KSGSPFLLH AAAKPPTTP TLKFHNHFP 
      PDADKKDNI LDNSLTTDG SVFSPPLKR TKMLTGPGS 
      AGPPPLHER VMLYVRQEV DDVYTPLHV VPPTTAGLL 
      NAIENKYKI SASSINNLY RKNKKGITA KIDDDMVAY 
      YCNEDLFLL ELKIVEEDL FDITFVELS DH
    • >TC034531 RB
      MTRRNMEDM PLNSSPSLE ADGSMMSPP PPQASPADR 
      SPHQDPPES PAMLATMTP VNVIELKKE MEEESAMAR 
      APLYAQVKT EVISYDKDE LYAQPLPDK NDTPIDLIY 
      EDGNKTVIY TTAFDQKSL EIYSADNGG EITINNLSA 
      HQIHSEGLI DGNSGAIAG GEPTGSPPG TVSVVLQGN 
      QGLLQYPTQ SQVPTVLVL SEMTEDEGV PVIGLRKRR 
      DNLESEGNS LHQSSSGGE QLPVVVQRG MAELPEGAA 
      PPEDQHPLT AATTAMLNI SGGAEDQST AMGLIYEYY 
      KFQPISGES KDKLADIWP ASSQAGGIL TSQLKATNG 
      LVSGAGLEL PTPPQLSSQ DWQGLFINQ HVGYPQAAA 
      SAQNFQLVK REPEDLSHH RKLDCSSPS SVSLDGAII 
      NTKQQRHKV VLPAMYATQ IFAAMTNNG HGGSGTPSP 
      TPYSDHVTG YTQSGAGYA AASRTATSA AAFVSDPYY 
      REYFTVASG GATPEPAYS GQLRQSQAY SEEVGGGTT 
      ASFVERYVR QPYHSKGVI AAATAAGLT VDLPSPDSG 
      IGADAITPR DQNTLQQNY EYPEICPNP NILDPLTIS 
      QRNSSSSQS PGSQSTSRS RPWHDFGRQ NDADKIQIP 
      KIFSPVGFK YQLETPIST SQRREDDRI TYINKGQFY 
      GITLDYIPD PDKQLKSQT VKSIVMLMF REEKSPEDE 
      IKAWQFWHG RQHSVKQRI LDADTKNSV GLVGCIEEV 
      AHNAIAIYW NPLESPAKI NIAVQCLST DFSSQKGVK 
      GLPLHLQID TYEDPRDTN VYHRGYCQI KVFCDKGAE 
      RKTRDEERR AAKRKMTAT GRKKLDELY HPMCERSEF 
      YSMSDLAKP PVLFSPAED IDKLTSMEL QGFYPHDTD 
      SSLTGPVDH KSGSPFLLH AAAKPPTTP TLKFHNHFP 
      PDADKKDNI LDNSLTTDG SVFSPPLKR TKMLTGPGS 
      AGPPPLHER VMLYVRQEV DDVYTPLHV VPPTTAGLL 
      NAHYGQD
    CDS sequence
    • >TC034531 RA
      atgactcgc cgaaacatg gaagatatg cctttgaat 
      tcgtcgcca tcgctcgaa gccgacggg agcatgatg 
      tctcctccg ccgccgcaa gcctcgcca gccgaccgc 
      tcgccacac caagaccct ccggagtct ccggcgatg 
      ctggccacc atgaccccc gtgaacgtg atcgagctc 
      aagaaggag atggaggag gaaagtgcc atggcaagg 
      gccccgctg tacgcccaa gtcaaaacc gaagtcatc 
      tcctacgac aaagacgaa ctctacgcg caaccgctc 
      cccgacaag aacgacacc cccatcgac ctgatctac 
      gaagatggc aacaagacc gtcatctac accaccgcg 
      ttcgaccag aaaagcctc gaaatttac tcggccgat 
      aacgggggc gaaatcacc atcaataat ttaagtgcc 
      catcagatt cacagcgag gggttaatc gacgggaat 
      tcgggcgcg atcgcgggg ggagaaccc accgggagt 
      cccccgggg acagtgtca gtggtactg cagggaaac 
      cagggactt ctccaatac ccgactcag agtcaagtg 
      cccaccgtt ttggtcctt tccgagatg acggaggac 
      gagggagtg cccgttatt ggactcagg aaacggaga 
      gataattta gagtctgag ggcaattcg cttcatcaa 
      tctagcagt ggcggagaa cagttgccc gtagttgtt 
      caacggggt atggcagag ttgcccgag ggggcggcg 
      cctcctgag gaccagcac ccgttgaca gccgccaca 
      accgccatg ttgaacatc agcggcggc gccgaggac 
      cagtccacg gccatgggg ctcatttac gaatattac 
      aaatttcag cccatttcc ggggaatcc aaagacaaa 
      ctcgcagat atctggccg gcaagttcc caggccgga 
      ggcatacta acgtcgcaa ctgaaggcg accaatggc 
      ctggtgagt ggggcgggg ctagagctg ccgacccct 
      ccccagtta agttcgcaa gactggcaa ggtttattt 
      attaaccaa cacgtcggg tacccacag gccgccgcc 
      tctgcccag aacttccaa ctcgtgaag cgcgaaccg 
      gaggacttg agccaccac cggaagctg gactgctcg 
      tcgcccagt tccgtcagt ttggacggg gccatcatc 
      aacaccaag cagcagagg cacaaggtc gtgctgccg 
      gccatgtac gccacccag atattcgcc gccatgacg 
      aacaacggc cacgggggc agcggcacc ccctcgccc 
      accccctac agcgatcac gtgacgggc tacacgcag 
      agcggcgcc ggctacgcc gccgccagc cgcaccgcc 
      acctcggcg gcggcgttc gtctccgac ccgtactac 
      cgcgagtac ttcacggtc gcctccggc ggcgccacc 
      cccgagccc gcctactcg gggcagctg cgccagagc 
      caggcctac agtgaggag gtcggcggc ggcaccacc 
      gccagtttc gtcgagcga tacgtcagg cagccctac 
      cacagcaag ggcgtcatc gccgccgcc accgccgcc 
      ggactcact gtcgacctg ccgagtccc gacagcggc 
      atcggcgcc gacgccatc accccccgc gaccagaac 
      accctgcag cagaactac gagtaccca gagatctgt 
      ccgaatccg aacattttg gaccctttg acgatttcg 
      caacggaac agctcgagt tcgcaatct cctggatct 
      caatcgacg agtcgaagt cggccttgg cacgatttc 
      ggccgtcaa aacgacgcc gataaaatc caaatccca 
      aaaatattt tcgccggtg ggcttcaag taccagttg 
      gaaacgccg atttcgacg tcgcagagg cgcgaagac 
      gacaggatc acgtacata aacaaaggc caattttac 
      ggaattact ctagactac atcccagac cctgacaag 
      cagctcaaa tcgcaaaca gtaaagagc atcgtgatg 
      ctgatgttc cgagaagag aaaagcccc gaagacgag 
      atcaaagcc tggcagttt tggcacggc cgccaacat 
      tccgttaaa cagcgaatt ttagatgca gatacgaaa 
      aatagcgtg ggtttagtc ggctgtata gaagaagta 
      gcacataac gctatagcc atctattgg aatccgtta 
      gaaagtccc gcaaagata aacatagca gtgcaatgt 
      ttaagtacc gactttagt agccaaaag ggcgtcaaa 
      ggcttaccg ttacattta caaattgat acgtacgaa 
      gacccccgg gatacaaac gtctaccac cgcggttac 
      tgccaaata aaagtattt tgtgataag ggagcggag 
      cgcaagaca cgtgacgag gagcgaagg gcagcaaaa 
      cgaaaaatg accgcaact ggtcgaaaa aaactggac 
      gaattgtat caccccatg tgcgagcgc tccgaattt 
      tactcaatg agcgacctc gcgaagccc cccgtgttg 
      ttctccccc gccgaagac atcgacaaa ttgacgtcg 
      atggaatta cagggtttt tacccccac gacactgac 
      agttctttg acgggccct gttgaccac aagtctggc 
      tctccgttc ctcctgcat gccgccgcc aagcccccg 
      accaccccc acgctcaaa ttccataac cattttccc 
      cccgacgcc gacaagaaa gacaatatt ttggataat 
      agtttgaca acggacggg agcgtcttt tcgccccct 
      ttgaagagg accaagatg ctcacgggg cctggcagc 
      gcggggcct ccgcccctg cacgaaagg gtgatgctt 
      tacgtgcgc caggaagtg gatgatgtc tacacaccc 
      ctacatgta gtgccgccc accacggcg gggctgctc 
      aatgcgatt gagaataag tacaaaata agcgcttcg 
      agcataaat aacttgtat cgcaaaaac aaaaaaggc 
      attacggcc aagattgat gacgacatg gtggcgtat 
      tattgcaac gaagacctt tttttgctc gagcttaag 
      atcgtggaa gaagaccta tttgatatt acttttgta 
      gaactctct gaccattga
    • >TC034531 RB
      atgactcgc cgaaacatg gaagatatg cctttgaat 
      tcgtcgcca tcgctcgaa gccgacggg agcatgatg 
      tctcctccg ccgccgcaa gcctcgcca gccgaccgc 
      tcgccacac caagaccct ccggagtct ccggcgatg 
      ctggccacc atgaccccc gtgaacgtg atcgagctc 
      aagaaggag atggaggag gaaagtgcc atggcaagg 
      gccccgctg tacgcccaa gtcaaaacc gaagtcatc 
      tcctacgac aaagacgaa ctctacgcg caaccgctc 
      cccgacaag aacgacacc cccatcgac ctgatctac 
      gaagatggc aacaagacc gtcatctac accaccgcg 
      ttcgaccag aaaagcctc gaaatttac tcggccgat 
      aacgggggc gaaatcacc atcaataat ttaagtgcc 
      catcagatt cacagcgag gggttaatc gacgggaat 
      tcgggcgcg atcgcgggg ggagaaccc accgggagt 
      cccccgggg acagtgtca gtggtactg cagggaaac 
      cagggactt ctccaatac ccgactcag agtcaagtg 
      cccaccgtt ttggtcctt tccgagatg acggaggac 
      gagggagtg cccgttatt ggactcagg aaacggaga 
      gataattta gagtctgag ggcaattcg cttcatcaa 
      tctagcagt ggcggagaa cagttgccc gtagttgtt 
      caacggggt atggcagag ttgcccgag ggggcggcg 
      cctcctgag gaccagcac ccgttgaca gccgccaca 
      accgccatg ttgaacatc agcggcggc gccgaggac 
      cagtccacg gccatgggg ctcatttac gaatattac 
      aaatttcag cccatttcc ggggaatcc aaagacaaa 
      ctcgcagat atctggccg gcaagttcc caggccgga 
      ggcatacta acgtcgcaa ctgaaggcg accaatggc 
      ctggtgagt ggggcgggg ctagagctg ccgacccct 
      ccccagtta agttcgcaa gactggcaa ggtttattt 
      attaaccaa cacgtcggg tacccacag gccgccgcc 
      tctgcccag aacttccaa ctcgtgaag cgcgaaccg 
      gaggacttg agccaccac cggaagctg gactgctcg 
      tcgcccagt tccgtcagt ttggacggg gccatcatc 
      aacaccaag cagcagagg cacaaggtc gtgctgccg 
      gccatgtac gccacccag atattcgcc gccatgacg 
      aacaacggc cacgggggc agcggcacc ccctcgccc 
      accccctac agcgatcac gtgacgggc tacacgcag 
      agcggcgcc ggctacgcc gccgccagc cgcaccgcc 
      acctcggcg gcggcgttc gtctccgac ccgtactac 
      cgcgagtac ttcacggtc gcctccggc ggcgccacc 
      cccgagccc gcctactcg gggcagctg cgccagagc 
      caggcctac agtgaggag gtcggcggc ggcaccacc 
      gccagtttc gtcgagcga tacgtcagg cagccctac 
      cacagcaag ggcgtcatc gccgccgcc accgccgcc 
      ggactcact gtcgacctg ccgagtccc gacagcggc 
      atcggcgcc gacgccatc accccccgc gaccagaac 
      accctgcag cagaactac gagtaccca gagatctgt 
      ccgaatccg aacattttg gaccctttg acgatttcg 
      caacggaac agctcgagt tcgcaatct cctggatct 
      caatcgacg agtcgaagt cggccttgg cacgatttc 
      ggccgtcaa aacgacgcc gataaaatc caaatccca 
      aaaatattt tcgccggtg ggcttcaag taccagttg 
      gaaacgccg atttcgacg tcgcagagg cgcgaagac 
      gacaggatc acgtacata aacaaaggc caattttac 
      ggaattact ctagactac atcccagac cctgacaag 
      cagctcaaa tcgcaaaca gtaaagagc atcgtgatg 
      ctgatgttc cgagaagag aaaagcccc gaagacgag 
      atcaaagcc tggcagttt tggcacggc cgccaacat 
      tccgttaaa cagcgaatt ttagatgca gatacgaaa 
      aatagcgtg ggtttagtc ggctgtata gaagaagta 
      gcacataac gctatagcc atctattgg aatccgtta 
      gaaagtccc gcaaagata aacatagca gtgcaatgt 
      ttaagtacc gactttagt agccaaaag ggcgtcaaa 
      ggcttaccg ttacattta caaattgat acgtacgaa 
      gacccccgg gatacaaac gtctaccac cgcggttac 
      tgccaaata aaagtattt tgtgataag ggagcggag 
      cgcaagaca cgtgacgag gagcgaagg gcagcaaaa 
      cgaaaaatg accgcaact ggtcgaaaa aaactggac 
      gaattgtat caccccatg tgcgagcgc tccgaattt 
      tactcaatg agcgacctc gcgaagccc cccgtgttg 
      ttctccccc gccgaagac atcgacaaa ttgacgtcg 
      atggaatta cagggtttt tacccccac gacactgac 
      agttctttg acgggccct gttgaccac aagtctggc 
      tctccgttc ctcctgcat gccgccgcc aagcccccg 
      accaccccc acgctcaaa ttccataac cattttccc 
      cccgacgcc gacaagaaa gacaatatt ttggataat 
      agtttgaca acggacggg agcgtcttt tcgccccct 
      ttgaagagg accaagatg ctcacgggg cctggcagc 
      gcggggcct ccgcccctg cacgaaagg gtgatgctt 
      tacgtgcgc caggaagtg gatgatgtc tacacaccc 
      ctacatgta gtgccgccc accacggcg gggctgctc 
      aatgcgcat tacggccaa gattga
    mRNA sequence
    • >TC034531 RA
      agtggaatc agcggcgcc caatggact cccacgtgc 
      agtgagttg gacggagag ggcctgagc tgtcgggtg 
      gtgtcgctg cgggggccc ggaggcgac tcggctacg 
      aacacagcg agcggcctt cgaagcaag caaagacac 
      aagtcttgg ggccggcat tcactttcc ctgcgcccg 
      agtaagacg gagaaccga acgcaagcc cgagatggt 
      ctttggctt cgattggct ttgagctgc cgcaactaa 
      cagtcagta gagtattag ttcgaaaca cctgtagac 
      gctagcatg tgattgaaa gtagaggcc cgacgagac 
      aaatatgtt ggcaaaacg tgggggaca cggacctgg 
      acagtgtgc agcagtgta gtgagtttg tgtcgccaa 
      aacaaagga cttcacgcg agatcaccc accatcagc 
      catacaagt gccataccg aacaatcat acgtaaaaa 
      gtgcaatcg cgttaagtg actgtgata aaaactcaa 
      gccataatg actcgccga aacatggaa gatatgcct 
      ttgaattcg tcgccatcg ctcgaagcc gacgggagc 
      atgatgtct cctccgccg ccgcaagcc tcgccagcc 
      gaccgctcg ccacaccaa gaccctccg gagtctccg 
      gcgatgctg gccaccatg acccccgtg aacgtgatc 
      gagctcaag aaggagatg gaggaggaa agtgccatg 
      gcaagggcc ccgctgtac gcccaagtc aaaaccgaa 
      gtcatctcc tacgacaaa gacgaactc tacgcgcaa 
      ccgctcccc gacaagaac gacaccccc atcgacctg 
      atctacgaa gatggcaac aagaccgtc atctacacc 
      accgcgttc gaccagaaa agcctcgaa atttactcg 
      gccgataac gggggcgaa atcaccatc aataattta 
      agtgcccat cagattcac agcgagggg ttaatcgac 
      gggaattcg ggcgcgatc gcgggggga gaacccacc 
      gggagtccc ccggggaca gtgtcagtg gtactgcag 
      ggaaaccag ggacttctc caatacccg actcagagt 
      caagtgccc accgttttg gtcctttcc gagatgacg 
      gaggacgag ggagtgccc gttattgga ctcaggaaa 
      cggagagat aatttagag tctgagggc aattcgctt 
      catcaatct agcagtggc ggagaacag ttgcccgta 
      gttgttcaa cggggtatg gcagagttg cccgagggg 
      gcggcgcct cctgaggac cagcacccg ttgacagcc 
      gccacaacc gccatgttg aacatcagc ggcggcgcc 
      gaggaccag tccacggcc atggggctc atttacgaa 
      tattacaaa tttcagccc atttccggg gaatccaaa 
      gacaaactc gcagatatc tggccggca agttcccag 
      gccggaggc atactaacg tcgcaactg aaggcgacc 
      aatggcctg gtgagtggg gcggggcta gagctgccg 
      acccctccc cagttaagt tcgcaagac tggcaaggt 
      ttatttatt aaccaacac gtcgggtac ccacaggcc 
      gccgcctct gcccagaac ttccaactc gtgaagcgc 
      gaaccggag gacttgagc caccaccgg aagctggac 
      tgctcgtcg cccagttcc gtcagtttg gacggggcc 
      atcatcaac accaagcag cagaggcac aaggtcgtg 
      ctgccggcc atgtacgcc acccagata ttcgccgcc 
      atgacgaac aacggccac gggggcagc ggcaccccc 
      tcgcccacc ccctacagc gatcacgtg acgggctac 
      acgcagagc ggcgccggc tacgccgcc gccagccgc 
      accgccacc tcggcggcg gcgttcgtc tccgacccg 
      tactaccgc gagtacttc acggtcgcc tccggcggc 
      gccaccccc gagcccgcc tactcgggg cagctgcgc 
      cagagccag gcctacagt gaggaggtc ggcggcggc 
      accaccgcc agtttcgtc gagcgatac gtcaggcag 
      ccctaccac agcaagggc gtcatcgcc gccgccacc 
      gccgccgga ctcactgtc gacctgccg agtcccgac 
      agcggcatc ggcgccgac gccatcacc ccccgcgac 
      cagaacacc ctgcagcag aactacgag tacccagag 
      atctgtccg aatccgaac attttggac cctttgacg 
      atttcgcaa cggaacagc tcgagttcg caatctcct 
      ggatctcaa tcgacgagt cgaagtcgg ccttggcac 
      gatttcggc cgtcaaaac gacgccgat aaaatccaa 
      atcccaaaa atattttcg ccggtgggc ttcaagtac 
      cagttggaa acgccgatt tcgacgtcg cagaggcgc 
      gaagacgac aggatcacg tacataaac aaaggccaa 
      ttttacgga attactcta gactacatc ccagaccct 
      gacaagcag ctcaaatcg caaacagta aagagcatc 
      gtgatgctg atgttccga gaagagaaa agccccgaa 
      gacgagatc aaagcctgg cagttttgg cacggccgc 
      caacattcc gttaaacag cgaatttta gatgcagat 
      acgaaaaat agcgtgggt ttagtcggc tgtatagaa 
      gaagtagca cataacgct atagccatc tattggaat 
      ccgttagaa agtcccgca aagataaac atagcagtg 
      caatgttta agtaccgac tttagtagc caaaagggc 
      gtcaaaggc ttaccgtta catttacaa attgatacg 
      tacgaagac ccccgggat acaaacgtc taccaccgc 
      ggttactgc caaataaaa gtattttgt gataaggga 
      gcggagcgc aagacacgt gacgaggag cgaagggca 
      gcaaaacga aaaatgacc gcaactggt cgaaaaaaa 
      ctggacgaa ttgtatcac cccatgtgc gagcgctcc 
      gaattttac tcaatgagc gacctcgcg aagcccccc 
      gtgttgttc tcccccgcc gaagacatc gacaaattg 
      acgtcgatg gaattacag ggtttttac ccccacgac 
      actgacagt tctttgacg ggccctgtt gaccacaag 
      tctggctct ccgttcctc ctgcatgcc gccgccaag 
      cccccgacc acccccacg ctcaaattc cataaccat 
      tttcccccc gacgccgac aagaaagac aatattttg 
      gataatagt ttgacaacg gacgggagc gtcttttcg 
      ccccctttg aagaggacc aagatgctc acggggcct 
      ggcagcgcg gggcctccg cccctgcac gaaagggtg 
      atgctttac gtgcgccag gaagtggat gatgtctac 
      acaccccta catgtagtg ccgcccacc acggcgggg 
      ctgctcaat gcgattgag aataagtac aaaataagc 
      gcttcgagc ataaataac ttgtatcgc aaaaacaaa 
      aaaggcatt acggccaag attgatgac gacatggtg 
      gcgtattat tgcaacgaa gaccttttt ttgctcgag 
      cttaagatc gtggaagaa gacctattt gatattact 
      tttgtagaa ctctctgac cattgaaac aattgccat 
      atattaaag taacgtact cccgttttt attcatcta 
      cttttgtga ttgtttttt acaatgtaa tattagtta 
      cttatagat tttttattg tagaatttt ataattgtt 
      catagtatt tagactcaa tcatgttta tcagttttg 
      ttagtgtgt tgtttttat ttttcttat gatctttat 
      ttataaaat ataaaattg atcattaac atccaatgt 
      tctttttta tttatcaaa taatataaa aaatgactc 
      gatgtagca ttgaaattt tatttacat ggatcaatg 
      tttatttta ttgttttct tttttagat gttttcttt 
      gttagcttg tttttccga cgctatttg gtcgagttt 
      gaccaatga tctcaaaat tgaatcatg taaatgatc 
      tgagggttg ggaataagg aaattactc attgtatat 
      agattagag ttttatgac atgtacata gatcaagcc 
      atgacctag actagatac attccttat tgtgataag 
      aatacgaac attgtagcc cagacattc atattgcga 
      gtatcaaga atttcgcaa aaattataa gtacctgag 
      ttatgcttt atcataata taatttttc gaacatttc 
      ctcaaaaaa taaagttat tttgggtac gattaactg 
      actcttcca taaattagg atgggaata ttcatactc 
      ccgacattc cacatggta ctgaatgac taaatcata 
      gtcgtgtat atttcaagt aatttattg ctcgtatgc 
      aacaattgt agtctgttg catacatgt tatcatgat 
      attagccta gtttagact taagtaaga tagctctct 
      gcatgtagt tctacctat gtaagagta ctataacta 
      tcataacta aatgataag ttaatga
    • >TC034531 RB
      agtggaatc agcggcgcc caatggact cccacgtgc 
      agtgagttg gacggagag ggcctgagc tgtcgggtg 
      gtgtcgctg cgggggccc ggaggcgac tcggctacg 
      aacacagcg agcggcctt cgaagcaag caaagacac 
      aagtcttgg ggccggcat tcactttcc ctgcgcccg 
      agtaagacg gagaaccga acgcaagcc cgagatggt 
      ctttggctt cgattggct ttgagctgc cgcaactaa 
      cagtcagta gagtattag ttcgaaaca cctgtagac 
      gctagcatg tgattgaaa gtagaggcc cgacgagac 
      aaatatgtt ggcaaaacg tgggggaca cggacctgg 
      acagtgtgc agcagtgta gtgagtttg tgtcgccaa 
      aacaaagga cttcacgcg agatcaccc accatcagc 
      catacaagt gccataccg aacaatcat acgtaaaaa 
      gtgcaatcg cgttaagtg actgtgata aaaactcaa 
      gccataatg actcgccga aacatggaa gatatgcct 
      ttgaattcg tcgccatcg ctcgaagcc gacgggagc 
      atgatgtct cctccgccg ccgcaagcc tcgccagcc 
      gaccgctcg ccacaccaa gaccctccg gagtctccg 
      gcgatgctg gccaccatg acccccgtg aacgtgatc 
      gagctcaag aaggagatg gaggaggaa agtgccatg 
      gcaagggcc ccgctgtac gcccaagtc aaaaccgaa 
      gtcatctcc tacgacaaa gacgaactc tacgcgcaa 
      ccgctcccc gacaagaac gacaccccc atcgacctg 
      atctacgaa gatggcaac aagaccgtc atctacacc 
      accgcgttc gaccagaaa agcctcgaa atttactcg 
      gccgataac gggggcgaa atcaccatc aataattta 
      agtgcccat cagattcac agcgagggg ttaatcgac 
      gggaattcg ggcgcgatc gcgggggga gaacccacc 
      gggagtccc ccggggaca gtgtcagtg gtactgcag 
      ggaaaccag ggacttctc caatacccg actcagagt 
      caagtgccc accgttttg gtcctttcc gagatgacg 
      gaggacgag ggagtgccc gttattgga ctcaggaaa 
      cggagagat aatttagag tctgagggc aattcgctt 
      catcaatct agcagtggc ggagaacag ttgcccgta 
      gttgttcaa cggggtatg gcagagttg cccgagggg 
      gcggcgcct cctgaggac cagcacccg ttgacagcc 
      gccacaacc gccatgttg aacatcagc ggcggcgcc 
      gaggaccag tccacggcc atggggctc atttacgaa 
      tattacaaa tttcagccc atttccggg gaatccaaa 
      gacaaactc gcagatatc tggccggca agttcccag 
      gccggaggc atactaacg tcgcaactg aaggcgacc 
      aatggcctg gtgagtggg gcggggcta gagctgccg 
      acccctccc cagttaagt tcgcaagac tggcaaggt 
      ttatttatt aaccaacac gtcgggtac ccacaggcc 
      gccgcctct gcccagaac ttccaactc gtgaagcgc 
      gaaccggag gacttgagc caccaccgg aagctggac 
      tgctcgtcg cccagttcc gtcagtttg gacggggcc 
      atcatcaac accaagcag cagaggcac aaggtcgtg 
      ctgccggcc atgtacgcc acccagata ttcgccgcc 
      atgacgaac aacggccac gggggcagc ggcaccccc 
      tcgcccacc ccctacagc gatcacgtg acgggctac 
      acgcagagc ggcgccggc tacgccgcc gccagccgc 
      accgccacc tcggcggcg gcgttcgtc tccgacccg 
      tactaccgc gagtacttc acggtcgcc tccggcggc 
      gccaccccc gagcccgcc tactcgggg cagctgcgc 
      cagagccag gcctacagt gaggaggtc ggcggcggc 
      accaccgcc agtttcgtc gagcgatac gtcaggcag 
      ccctaccac agcaagggc gtcatcgcc gccgccacc 
      gccgccgga ctcactgtc gacctgccg agtcccgac 
      agcggcatc ggcgccgac gccatcacc ccccgcgac 
      cagaacacc ctgcagcag aactacgag tacccagag 
      atctgtccg aatccgaac attttggac cctttgacg 
      atttcgcaa cggaacagc tcgagttcg caatctcct 
      ggatctcaa tcgacgagt cgaagtcgg ccttggcac 
      gatttcggc cgtcaaaac gacgccgat aaaatccaa 
      atcccaaaa atattttcg ccggtgggc ttcaagtac 
      cagttggaa acgccgatt tcgacgtcg cagaggcgc 
      gaagacgac aggatcacg tacataaac aaaggccaa 
      ttttacgga attactcta gactacatc ccagaccct 
      gacaagcag ctcaaatcg caaacagta aagagcatc 
      gtgatgctg atgttccga gaagagaaa agccccgaa 
      gacgagatc aaagcctgg cagttttgg cacggccgc 
      caacattcc gttaaacag cgaatttta gatgcagat 
      acgaaaaat agcgtgggt ttagtcggc tgtatagaa 
      gaagtagca cataacgct atagccatc tattggaat 
      ccgttagaa agtcccgca aagataaac atagcagtg 
      caatgttta agtaccgac tttagtagc caaaagggc 
      gtcaaaggc ttaccgtta catttacaa attgatacg 
      tacgaagac ccccgggat acaaacgtc taccaccgc 
      ggttactgc caaataaaa gtattttgt gataaggga 
      gcggagcgc aagacacgt gacgaggag cgaagggca 
      gcaaaacga aaaatgacc gcaactggt cgaaaaaaa 
      ctggacgaa ttgtatcac cccatgtgc gagcgctcc 
      gaattttac tcaatgagc gacctcgcg aagcccccc 
      gtgttgttc tcccccgcc gaagacatc gacaaattg 
      acgtcgatg gaattacag ggtttttac ccccacgac 
      actgacagt tctttgacg ggccctgtt gaccacaag 
      tctggctct ccgttcctc ctgcatgcc gccgccaag 
      cccccgacc acccccacg ctcaaattc cataaccat 
      tttcccccc gacgccgac aagaaagac aatattttg 
      gataatagt ttgacaacg gacgggagc gtcttttcg 
      ccccctttg aagaggacc aagatgctc acggggcct 
      ggcagcgcg gggcctccg cccctgcac gaaagggtg 
      atgctttac gtgcgccag gaagtggat gatgtctac 
      acaccccta catgtagtg ccgcccacc acggcgggg 
      ctgctcaat gcgcattac ggccaagat tgatgacga 
      catggtggc gtattattg caacgaaga ccttttttt 
      gctcgagct taagatcgt ggaagaaga cctatttga 
      tattacttt tgtagaact ctctgacca ttgaaacaa 
      ttgccatat attaaagta acgtactcc cgtttttat 
      tcatctact tttgtgatt gttttttac aatgtaata 
      ttagttact tatagattt tttattgta gaattttat 
      aattgttca tagtattta gactcaatc atgtttatc 
      agttttgtt agtgtgttg tttttattt ttcttatga 
      tctttattt ataaaatat aaaattgat cattaacat 
      ccaatgttc ttttttatt tatcaaata atataaaaa 
      atgactcga tgtagcatt gaaatttta tttacatgg 
      atcaatgtt tattttatt gttttcttt tttagatgt 
      tttctttgt tagcttgtt tttccgacg ctatttggt 
      cgagtttga ccaatgatc tcaaaattg aatcatgta 
      aatgatctg agggttggg aataaggaa attactcat 
      tgtatatag attagagtt ttatgacat gtacataga 
      tcaagccat gacctagac tagatacat tccttattg 
      tgataagaa tacgaacat tgtagccca gacattcat 
      attgcgagt atcaagaat ttcgcaaaa attataagt 
      acctgagtt atgctttat cataatata atttttcga 
      acatttcct caaaaaata aagttattt tgggtacga 
      ttaactgac tcttccata aattaggat gggaatatt 
      catactccc gacattcca catggtact gaatgacta 
      aatcatagt cgtgtatat ttcaagtaa tttattgct 
      cgtatgcaa caattgtag tctgttgca tacatgtta 
      tcatgatat tagcctagt ttagactta agtaagata 
      gctctctgc atgtagttc tacctatgt aagagtact 
      ataactatc ataactaaa tgataagtt aatga
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