Status
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Gene information
General information
TC034542 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2139353..2146377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034542
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03791

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034542 RA
      MSQLESHCE HEEKPEEDI IQSTIGNFG RWQFKISAL 
      MALLKFPIA WFQMSIVFL APPTQFWCK SNESSTNEW 
      LKMSQQNHT LRHEGLNSG FCQSLANNT LTTCQNGYE 
      YNRTVFNSS IITEWNLVC ENKRLVDVS QISLMFGVL 
      LGNILFGVI ADRKGRKKT LLVCVVFQS LLGIAASQI 
      PWYWGFIVT RFLLAIANG GTIVISFVM CMEVVGGTW 
      RSIVPILYQ IPYGFGNSI MAGIAYFHR DWRDLHLVL 
      SLISSFYIL YLWLVPESP RWLLATGNK SEAIKILER 
      AAQCNKMDS DKVKTVAER LSPDAKSES KPKLAALFA 
      TTELTKRNV LLCINWLIA GITFYAFSQ YIGKVGSNL 
      YFTAAISGF VAFPGTLLC VFIIRRFGR RLTIASAHV 
      LTAICFFGI LAVPLDVYY QDWPRVVIA GIGIVGLSI 
      SMPALYLFT GELFPTTVR NAGVGTSVM FSRMGSMIS 
      PLVISMQDI SPSLPLIVL GTGAILETF LILLLPETK 
      GMPLPETID DLSVTVNKT DDRKDIRMC YISLPTILK 
      DTSLDINK
    CDS sequence
    • >TC034542 RA
      atgtctcaa ctagagagt cactgtgaa catgaagaa 
      aaaccggaa gaagacatt attcaatcc acgataggt 
      aatttcggg cgatggcaa ttcaaaatc tcggcccta 
      atggccctt cttaaattt ccaatcgct tggtttcaa 
      atgagtatc gtttttctg gccccacca acacaattc 
      tggtgtaaa tcgaacgaa agttccaca aacgaatgg 
      ttgaagatg tcgcagcaa aaccacact ttaagacac 
      gaaggtctc aattctggc ttttgccaa agtcttgca 
      aataacaca ctgaccact tgccaaaac ggttacgag 
      tacaacaga accgttttc aactcaagt atcataaca 
      gaatggaat ttggtttgc gaaaacaag cgacttgtg 
      gatgtgtct caaatttct ctcatgttt ggagtttta 
      ttaggcaat attctgttt ggagtaatt gccgatcgt 
      aagggaagg aaaaaaact ttgcttgtt tgtgttgtt 
      ttccagtca cttctggga atcgcagca tcacaaatt 
      ccctggtat tgggggttc attgtgacg agatttctc 
      cttgccatt gctaatgga ggcacaatt gtcatttcg 
      tttgtgatg tgtatggaa gtagttggc ggaacatgg 
      cgcagtatt gtaccgatt ctgtaccaa ataccatat 
      gggtttggt aattcgata atggccggc attgcttac 
      tttcataga gattggagg gaccttcat ttagtattg 
      tcactaatt tcctctttc tacattttg tatttatgg 
      ttggttcct gaatcgcct cgttggtta ctagcaacg 
      gggaacaaa agcgaagct atcaaaatt ctcgaaagg 
      gcagcacaa tgtaacaaa atggacagt gataaagta 
      aaaactgtt gctgagaga ttatccccc gatgcaaaa 
      agtgaaagc aaacccaaa cttgccgca ctattcgcc 
      acaacagag ctaacgaaa agaaatgtt ttattgtgc 
      attaactgg ttaattgct ggaataacg ttctacgct 
      ttttctcaa tacatcgga aaagtgggt agcaatctt 
      tatttcaca gcagctatt agcggtttt gtggctttt 
      ccgggcact cttctgtgc gtcttcatt atacgacgt 
      ttcggacga agattgacc atagcctca gcacatgtt 
      ttgacggca atttgcttc tttgggatt cttgccgtt 
      cctttggat gtgtattat caagattgg ccccgagtt 
      gtgattgcg gggataggg attgtggga ttatcgatt 
      tctatgccg gcgttgtat ttatttact ggagagttg 
      ttcccaacg actgttaga aatgcagga gttggaacg 
      tcggtcatg ttttcaaga atgggttca atgatttct 
      cccttagtt atcagtatg caagatatt tcgccctct 
      ttgccgttg attgttttg ggtacaggg gctatttta 
      gaaacattt ctaatttta cttttgcca gaaactaaa 
      ggaatgccc cttcctgaa actattgat gatttgagc 
      gtaacagtc aataagact gatgataga aaggatatt 
      aggatgtgt tatatttcc ttgccaact attttgaaa 
      gacacttcc ttggatatt aacaaataa
    mRNA sequence
    • >TC034542 RA
      tatcaaaaa agaatctcg ctgatattt atttttatg 
      agacgcacg tagaatttc tcttctgca ttaaaatta 
      aaacagttg atttccgag ttcttctga ccacatgtg 
      cttgtgttt tctggacta tttttgaca tgtctcaac 
      tagagagtc actgtgaac atgaagaaa aaccggaag 
      aagacatta ttcaatcca cgataggta atttcgggc 
      gatggcaat tcaaaatct cggccctaa tggcccttc 
      ttaaatttc caatcgctt ggtttcaaa tgagtatcg 
      tttttctgg ccccaccaa cacaattct ggtgtaaat 
      cgaacgaaa gttccacaa acgaatggt tgaagatgt 
      cgcagcaaa accacactt taagacacg aaggtctca 
      attctggct tttgccaaa gtcttgcaa ataacacac 
      tgaccactt gccaaaacg gttacgagt acaacagaa 
      ccgttttca actcaagta tcataacag aatggaatt 
      tggtttgcg aaaacaagc gacttgtgg atgtgtctc 
      aaatttctc tcatgtttg gagttttat taggcaata 
      ttctgtttg gagtaattg ccgatcgta agggaagga 
      aaaaaactt tgcttgttt gtgttgttt tccagtcac 
      ttctgggaa tcgcagcat cacaaattc cctggtatt 
      gggggttca ttgtgacga gatttctcc ttgccattg 
      ctaatggag gcacaattg tcatttcgt ttgtgatgt 
      gtatggaag tagttggcg gaacatggc gcagtattg 
      taccgattc tgtaccaaa taccatatg ggtttggta 
      attcgataa tggccggca ttgcttact ttcatagag 
      attggaggg accttcatt tagtattgt cactaattt 
      cctctttct acattttgt atttatggt tggttcctg 
      aatcgcctc gttggttac tagcaacgg ggaacaaaa 
      gcgaagcta tcaaaattc tcgaaaggg cagcacaat 
      gtaacaaaa tggacagtg ataaagtaa aaactgttg 
      ctgagagat tatcccccg atgcaaaaa gtgaaagca 
      aacccaaac ttgccgcac tattcgcca caacagagc 
      taacgaaaa gaaatgttt tattgtgca ttaactggt 
      taattgctg gaataacgt tctacgctt tttctcaat 
      acatcggaa aagtgggta gcaatcttt atttcacag 
      cagctatta gcggttttg tggcttttc cgggcactc 
      ttctgtgcg tcttcatta tacgacgtt tcggacgaa 
      gattgacca tagcctcag cacatgttt tgacggcaa 
      tttgcttct ttgggattc ttgccgttc ctttggatg 
      tgtattatc aagattggc cccgagttg tgattgcgg 
      ggataggga ttgtgggat tatcgattt ctatgccgg 
      cgttgtatt tatttactg gagagttgt tcccaacga 
      ctgttagaa atgcaggag ttggaacgt cggtcatgt 
      tttcaagaa tgggttcaa tgatttctc ccttagtta 
      tcagtatgc aagatattt cgccctctt tgccgttga 
      ttgttttgg gtacagggg ctattttag aaacatttc 
      taattttac ttttgccag aaactaaag gaatgcccc 
      ttcctgaaa ctattgatg atttgagcg taacagtca 
      ataagactg atgatagaa aggatatta ggatgtgtt 
      atatttcct tgccaacta ttttgaaag acacttcct 
      tggatatta acaaataat atttgagaa aactgaaat 
      tatcatgct ttgaattgc ctttaatta ttgtgatct 
      tgtaacctt atgtaatac aaataaaaa tatttcatt 
      gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY