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Gene information
General information
TC034542 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2139353..2146377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034542
    iBeetle screen iB_03791

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034542 RA
      MSQLESHCE HEEKPEEDI IQSTIGNFG RWQFKISAL 
MALLKFPIA WFQMSIVFL APPTQFWCK SNESSTNEW 
LKMSQQNHT LRHEGLNSG FCQSLANNT LTTCQNGYE 
YNRTVFNSS IITEWNLVC ENKRLVDVS QISLMFGVL 
LGNILFGVI ADRKGRKKT LLVCVVFQS LLGIAASQI 
PWYWGFIVT RFLLAIANG GTIVISFVM CMEVVGGTW 
RSIVPILYQ IPYGFGNSI MAGIAYFHR DWRDLHLVL 
SLISSFYIL YLWLVPESP RWLLATGNK SEAIKILER 
AAQCNKMDS DKVKTVAER LSPDAKSES KPKLAALFA 
TTELTKRNV LLCINWLIA GITFYAFSQ YIGKVGSNL 
YFTAAISGF VAFPGTLLC VFIIRRFGR RLTIASAHV 
LTAICFFGI LAVPLDVYY QDWPRVVIA GIGIVGLSI 
SMPALYLFT GELFPTTVR NAGVGTSVM FSRMGSMIS 
PLVISMQDI SPSLPLIVL GTGAILETF LILLLPETK 
GMPLPETID DLSVTVNKT DDRKDIRMC YISLPTILK 
DTSLDINK
    CDS sequence
    • >TC034542 RA
      atgtctcaa ctagagagt cactgtgaa catgaagaa 
aaaccggaa gaagacatt attcaatcc acgataggt 
aatttcggg cgatggcaa ttcaaaatc tcggcccta 
atggccctt cttaaattt ccaatcgct tggtttcaa 
atgagtatc gtttttctg gccccacca acacaattc 
tggtgtaaa tcgaacgaa agttccaca aacgaatgg 
ttgaagatg tcgcagcaa aaccacact ttaagacac 
gaaggtctc aattctggc ttttgccaa agtcttgca 
aataacaca ctgaccact tgccaaaac ggttacgag 
tacaacaga accgttttc aactcaagt atcataaca 
gaatggaat ttggtttgc gaaaacaag cgacttgtg 
gatgtgtct caaatttct ctcatgttt ggagtttta 
ttaggcaat attctgttt ggagtaatt gccgatcgt 
aagggaagg aaaaaaact ttgcttgtt tgtgttgtt 
ttccagtca cttctggga atcgcagca tcacaaatt 
ccctggtat tgggggttc attgtgacg agatttctc 
cttgccatt gctaatgga ggcacaatt gtcatttcg 
tttgtgatg tgtatggaa gtagttggc ggaacatgg 
cgcagtatt gtaccgatt ctgtaccaa ataccatat 
gggtttggt aattcgata atggccggc attgcttac 
tttcataga gattggagg gaccttcat ttagtattg 
tcactaatt tcctctttc tacattttg tatttatgg 
ttggttcct gaatcgcct cgttggtta ctagcaacg 
gggaacaaa agcgaagct atcaaaatt ctcgaaagg 
gcagcacaa tgtaacaaa atggacagt gataaagta 
aaaactgtt gctgagaga ttatccccc gatgcaaaa 
agtgaaagc aaacccaaa cttgccgca ctattcgcc 
acaacagag ctaacgaaa agaaatgtt ttattgtgc 
attaactgg ttaattgct ggaataacg ttctacgct 
ttttctcaa tacatcgga aaagtgggt agcaatctt 
tatttcaca gcagctatt agcggtttt gtggctttt 
ccgggcact cttctgtgc gtcttcatt atacgacgt 
ttcggacga agattgacc atagcctca gcacatgtt 
ttgacggca atttgcttc tttgggatt cttgccgtt 
cctttggat gtgtattat caagattgg ccccgagtt 
gtgattgcg gggataggg attgtggga ttatcgatt 
tctatgccg gcgttgtat ttatttact ggagagttg 
ttcccaacg actgttaga aatgcagga gttggaacg 
tcggtcatg ttttcaaga atgggttca atgatttct 
cccttagtt atcagtatg caagatatt tcgccctct 
ttgccgttg attgttttg ggtacaggg gctatttta 
gaaacattt ctaatttta cttttgcca gaaactaaa 
ggaatgccc cttcctgaa actattgat gatttgagc 
gtaacagtc aataagact gatgataga aaggatatt 
aggatgtgt tatatttcc ttgccaact attttgaaa 
gacacttcc ttggatatt aacaaataa
    mRNA sequence
    • >TC034542 RA
      tatcaaaaa agaatctcg ctgatattt atttttatg 
agacgcacg tagaatttc tcttctgca ttaaaatta 
aaacagttg atttccgag ttcttctga ccacatgtg 
cttgtgttt tctggacta tttttgaca tgtctcaac 
tagagagtc actgtgaac atgaagaaa aaccggaag 
aagacatta ttcaatcca cgataggta atttcgggc 
gatggcaat tcaaaatct cggccctaa tggcccttc 
ttaaatttc caatcgctt ggtttcaaa tgagtatcg 
tttttctgg ccccaccaa cacaattct ggtgtaaat 
cgaacgaaa gttccacaa acgaatggt tgaagatgt 
cgcagcaaa accacactt taagacacg aaggtctca 
attctggct tttgccaaa gtcttgcaa ataacacac 
tgaccactt gccaaaacg gttacgagt acaacagaa 
ccgttttca actcaagta tcataacag aatggaatt 
tggtttgcg aaaacaagc gacttgtgg atgtgtctc 
aaatttctc tcatgtttg gagttttat taggcaata 
ttctgtttg gagtaattg ccgatcgta agggaagga 
aaaaaactt tgcttgttt gtgttgttt tccagtcac 
ttctgggaa tcgcagcat cacaaattc cctggtatt 
gggggttca ttgtgacga gatttctcc ttgccattg 
ctaatggag gcacaattg tcatttcgt ttgtgatgt 
gtatggaag tagttggcg gaacatggc gcagtattg 
taccgattc tgtaccaaa taccatatg ggtttggta 
attcgataa tggccggca ttgcttact ttcatagag 
attggaggg accttcatt tagtattgt cactaattt 
cctctttct acattttgt atttatggt tggttcctg 
aatcgcctc gttggttac tagcaacgg ggaacaaaa 
gcgaagcta tcaaaattc tcgaaaggg cagcacaat 
gtaacaaaa tggacagtg ataaagtaa aaactgttg 
ctgagagat tatcccccg atgcaaaaa gtgaaagca 
aacccaaac ttgccgcac tattcgcca caacagagc 
taacgaaaa gaaatgttt tattgtgca ttaactggt 
taattgctg gaataacgt tctacgctt tttctcaat 
acatcggaa aagtgggta gcaatcttt atttcacag 
cagctatta gcggttttg tggcttttc cgggcactc 
ttctgtgcg tcttcatta tacgacgtt tcggacgaa 
gattgacca tagcctcag cacatgttt tgacggcaa 
tttgcttct ttgggattc ttgccgttc ctttggatg 
tgtattatc aagattggc cccgagttg tgattgcgg 
ggataggga ttgtgggat tatcgattt ctatgccgg 
cgttgtatt tatttactg gagagttgt tcccaacga 
ctgttagaa atgcaggag ttggaacgt cggtcatgt 
tttcaagaa tgggttcaa tgatttctc ccttagtta 
tcagtatgc aagatattt cgccctctt tgccgttga 
ttgttttgg gtacagggg ctattttag aaacatttc 
taattttac ttttgccag aaactaaag gaatgcccc 
ttcctgaaa ctattgatg atttgagcg taacagtca 
ataagactg atgatagaa aggatatta ggatgtgtt 
atatttcct tgccaacta ttttgaaag acacttcct 
tggatatta acaaataat atttgagaa aactgaaat 
tatcatgct ttgaattgc ctttaatta ttgtgatct 
tgtaacctt atgtaatac aaataaaaa tatttcatt 
gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY