Status
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Gene information
General information
TC031960
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004554,TC004553
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2588283..2660067
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031960
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03783

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    elytra not closed - Number of animals showing the phenotype: 4


    Fertility
    egg production: number <50% of expected -
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031960 RA
      MLAVRIRNF LVCGCVLGT VVLSEKREG PLFNNEFAV 
      HIPGGNEVA DEIAAKHGF VNRGQVGSL KGFYLFEHK 
      HVHKRSLQA NDFYHQSLI SEPQVHWVQ QQHEKKRKK 
      RDYVFRPSV TPFSQYFSA AAGPPPGAL PSSSHSRLR 
      YRAAPSPGE FPDPLFKEQ WYLNGGAKD GLDMNLAPA 
      WKKGYTGKG VVVSILDDG IQTNHPDLA QNYDPLAST 
      DINGNDDDP MPQDNGDNK HGTRCAGEV AAVAYNQFC 
      GIGVAYNSS IGGVRMLDG VVNDAVEAR ALGLNPEHI 
      DIYSASWGP EDDGKTVDG PGPLARRAF IYGVTSGRK 
      GKGSIFVWA SGNGGRHTD SCNCDGYTN SIFTLSISS 
      ATQGGYKPW YLEECSSTL ATTYSSGTP GHDKSVATV 
      DMDARLRPD HICTVEHTG TSASAPLAA GICALALEA 
      NPSLTWRDM QYLVVLTSR SAPLEKESG WITNGVGRK 
      VSHKFGYGL MDAGALVSL AEKWTTVPP QHICKSQEL 
      VEDRQIDAA YGTTLDFHM DVDACSNSL NEVHFLEHV 
      QCKISLRFF PRGNLRIIL ISPMGTTST LLFERPRDV 
      VSSNFDDWP FLSVHFWGE RAEGRWTLR IVNAGNRHV 
      NQPGVLKKW QLIFYGTSV NPIRVRPAG RPQSSPWKS 
      PLNTFTFPT VSESVTQVT DNFFKDFHN FPNLYSFAG 
      SEPEQAVSS LDGKKTKIR DNDNNIDDE DEETKENCD 
      EQCDDQGCF GRGPTKCIA CRNKRMDRT CVSTCPPRS 
      YADSQGVCQ PCHEACETC TGSDQKSCL TCSPGHVRV 
      VDLDICLQQ CPEGYFQDF IAKTCTPCQ ANCANCQDR 
      PDHCTSCDH HLLLYQNKC LAFCPPNTY ETEDYTCAP 
      CHETCETCN GSNSSQCVS CTQGRFWHE NRCVKECPA 
      HHYADPHQH ECIECPPGC SECNKTTCI SCLDDWQVN 
      AKGRCVPHG SERCDIDQY FDGGLCKPC HSTCESCDG 
      PTEHACLSC ASPLILQDT RCVAVCDKT FFHDKGTRL 
      CEPCLHTCT KCVSKMNCS ECIPGLHLQ SGECSSTCA 
      TGYYSDKGI CARCYMSCK TCSGPAHNQ CVSCPQGWQ 
      LLAGECRPD CPEGYYKTE YGCQKCHYS CRNCIGCKV 
      QEDKRRIDV QALLLSAQP VSTLQLIGP FNFITGLAI 
      IAVLCVVIL FITVFIVLQ RNSIQAAPR GYSRVSIKK 
      PKVTCNYNV VAVSDGDSS DSDSSEGHN LVTKPKADR 
      TAC
    CDS sequence
    • >TC031960 RA
      atgttagca gtcagaata cgtaatttt ctcgtgtgc 
      gggtgtgtg ctcggcacg gtggtactt tcggaaaaa 
      cgcgaagga cccctcttc aacaacgag ttcgccgtt 
      catatccct ggaggtaac gaggtggcg gacgagatc 
      gccgccaag cacggattc gtcaatcgg ggacaggtc 
      ggcagcttg aagggtttc tacttgttc gaacacaag 
      cacgtgcat aaacgttcc ctgcaggcc aacgacttc 
      taccaccag tcgctgata tccgagccc caggtgcat 
      tgggttcaa cagcagcac gaaaagaag cggaaaaag 
      cgcgattac gtttttcgt ccatcggtc actccattc 
      tcgcagtat ttcagtgca gcagctgga ccaccacca 
      ggtgctttg ccctcttcg tctcacagc cgtctaagg 
      tatcgggcc gctccgtct cctggtgaa ttccccgat 
      cctctcttc aaagaacaa tggtatctg aacggcggt 
      gccaaagat ggattggac atgaatctg gcgccagcc 
      tggaagaaa ggatacact ggtaaggga gtcgtcgtg 
      tcaatcctg gacgacggc atacaaact aatcatccg 
      gatttggct caaaactac gaccctctg gccagcact 
      gacattaac ggcaacgac gacgatccc atgccgcaa 
      gataatgga gataataag cacggtacc cgttgtgcg 
      ggcgaagtg gctgctgtt gcttacaac caattttgc 
      ggaataggc gtcgcctac aactcgagc ataggaggt 
      gtacgaatg ctggacggc gtggtaaac gacgccgtc 
      gaggcccgc gccctgggc ctcaacccc gaacacatc 
      gacatttac agtgcttcc tggggcccc gaagacgac 
      ggcaaaacc gtcgacggc cctggacct ttagcccgt 
      cgtgccttc atttatggc gtgaccagc ggcaggaaa 
      ggcaaaggt tcgattttc gtttgggcg tctggaaac 
      ggtggaaga cacacagat tcgtgcaat tgcgacggc 
      tacaccaac agtattttc actttgagc atctctagt 
      gcgacccaa ggaggctac aaaccgtgg tatttggag 
      gagtgttcc tcgacgctt gccacaacg tacagttcc 
      ggaactcca ggtcatgat aaaagcgtc gcgacggtg 
      gatatggac gcgagactt cgccccgat catatttgc 
      acagttgaa catacgggg acttcggct tcggctccg 
      ttagcggcc ggaatttgt gccctagcg cttgaggca 
      aatccgagc ttgacttgg agggatatg caatatttg 
      gtggttttg acctcacgg tcggccccc ttggagaag 
      gaaagtggc tggattacg aatggggtt ggccggaaa 
      gtgtcgcat aaatttgga tatggactt atggatgct 
      ggagctttg gtaagtctt gccgagaaa tggactacg 
      gtaccaccg cagcacatc tgcaaatca caagaactg 
      gtcgaagat cggcaaatt gacgctgcg tatggtacg 
      actttggac ttccatatg gacgtggat gcttgtagt 
      aactcactg aacgaagtc cattttctg gaacacgtc 
      caatgtaag atttcgttg cggttcttc ccgcgtggc 
      aatttgcgc attattttg atttctcca atgggtacg 
      acttcaacg ttgttgttt gaaaggcca cgagacgtc 
      gtcagttcg aatttcgac gattggccg ttcttaagt 
      gttcatttc tggggcgaa agagctgaa ggccggtgg 
      actttacgt attgtaaat gctggtaat cgacacgtg 
      aaccaaccg ggagttttg aaaaaatgg caattgatt 
      ttttacggc acttctgtt aatcccata cgagtaaga 
      cctgcaggc aggcctcaa tctagtccc tggaagtcg 
      ccgcttaat acttttaca tttcctact gtctccgaa 
      tcggtgact caagtcacc gataatttc ttcaaagat 
      tttcacaac ttccccaat ctttattcg tttgctgga 
      tccgagccc gaacaagcc gtcagttcg ttggacggg 
      aaaaagacc aaaattcga gacaacgat aacaatatt 
      gacgacgaa gatgaagaa actaaagaa aactgtgat 
      gaacaatgt gatgatcaa ggttgtttc ggccgggga 
      ccgactaaa tgtatagct tgtagaaat aaacggatg 
      gataggact tgtgtcagc acttgtcca cctcgcagt 
      tacgccgat tcccagggt gtatgtcag ccgtgtcat 
      gaagcttgc gagacttgc accggatcg gaccaaaaa 
      agttgcctt acgtgttca ccaggtcac gtgagagtc 
      gtcgatctg gatatttgt ctacagcaa tgtcccgaa 
      ggctatttc caagatttc atcgcaaag acttgcacc 
      ccttgccaa gccaactgc gccaattgt caagaccgg 
      cccgaccat tgcaccagt tgcgaccac cacctcctt 
      ctctaccag aacaaatgc ctcgcgttt tgtcctccc 
      aatacgtac gaaaccgaa gattacacg tgtgcccct 
      tgtcacgaa acttgcgag acttgcaac ggttcgaat 
      agttctcag tgtgtttcc tgcacacaa ggacgcttc 
      tggcacgaa aatcgttgc gtgaaggaa tgccccgct 
      catcactac gccgaccca catcaacac gagtgtata 
      gaatgccct ccagggtgt tcggaatgc aacaaaacg 
      acctgcatt tcgtgtctg gacgattgg caagtgaac 
      gcgaagggc cgatgcgtg ccccacgga agcgaacgc 
      tgtgatata gaccaatat ttcgacggc ggcctgtgc 
      aagccctgc cactcgact tgcgaatcc tgcgacggc 
      cccaccgaa cacgcctgt ctttcctgc gccagccct 
      ctcatcctt caagacact cgttgcgtc gccgtctgt 
      gataaaacc ttctttcac gataaaggc acccgtctg 
      tgtgaaccc tgtctccat acttgcact aaatgcgtt 
      tcgaaaatg aactgtagc gagtgcata cccggatta 
      cacttgcaa agtggcgaa tgcagttcc acgtgtgcc 
      acaggctat tacagcgat aaaggaatc tgtgcgaga 
      tgttacatg agttgtaaa acgtgtagc ggtccggca 
      cacaatcag tgtgttagt tgtccgcaa ggttggcaa 
      cttttggcc ggggaatgc cggcctgat tgtccagag 
      gggtattac aagacggaa tatgggtgt caaaagtgc 
      cattattca tgcaggaat tgcataggg tgtaaggtg 
      caagaagac aaacgaagg attgacgta caagcttta 
      ttgctttca gcacaaccc gtatcgact ttacaattg 
      atcggcccg tttaatttc attacgggc ctggcaata 
      atcgccgtt ttatgtgta gtaatcctt tttataaca 
      gtttttata gttctacag agaaacagt attcaggca 
      gcgccgaga ggctacagc agggtatcc ataaaaaaa 
      cctaaagtt acatgtaat tacaatgtg gtggctgtg 
      agtgatggc gattcgtcg gattcggac agttcagaa 
      ggtcacaat ttagtaaca aagccgaaa gctgatcgg 
      actgcttgt taa
    mRNA sequence
    • >TC031960 RA
      acgtcaaaa cacaatcta gtgtgcagt tcgcagctg 
      acagtctgg ttgtcttgc gaactgctg tctgtcatt 
      tcgtcgaca atgttttcg tgatttttt taaacacac 
      agtatacga ttgtagttc gcgccgccc gaaatgcat 
      cccttgtag tgcagctcg aaatatgct acaagcggt 
      cctgtgtcg aaaattgtg cgagtgttg ccagcgatc 
      agctggaaa gtgtttttt agaaagtgg ctctgccgt 
      ttgtcaatt tcgcaagaa ttcgacttt cgatttttt 
      ccaaccctc cagtaccct atgatcgct ttggaaatt 
      ttccgcgta cagattgat catttttcc gggaatcgc 
      tcttcgaaa ccgtcacat aatgttaaa tagtatgtt 
      agcagtcag aatacgtaa ttttctcgt gtgcgggtg 
      tgtgctcgg cacggtggt actttcgga aaaacgcga 
      aggacccct cttcaacaa cgagttcgc cgttcatat 
      ccctggagg taacgaggt ggcggacga gatcgccgc 
      caagcacgg attcgtcaa tcggggaca ggtcggcag 
      cttgaaggg tttctactt gttcgaaca caagcacgt 
      gcataaacg ttccctgca ggccaacga cttctacca 
      ccagtcgct gatatccga gccccaggt gcattgggt 
      tcaacagca gcacgaaaa gaagcggaa aaagcgcga 
      ttacgtttt tcgtccatc ggtcactcc attctcgca 
      gtatttcag tgcagcagc tggaccacc accaggtgc 
      tttgccctc ttcgtctca cagccgtct aaggtatcg 
      ggccgctcc gtctcctgg tgaattccc cgatcctct 
      cttcaaaga acaatggta tctgaacgg cggtgccaa 
      agatggatt ggacatgaa tctggcgcc agcctggaa 
      gaaaggata cactggtaa gggagtcgt cgtgtcaat 
      cctggacga cggcataca aactaatca tccggattt 
      ggctcaaaa ctacgaccc tctggccag cactgacat 
      taacggcaa cgacgacga tcccatgcc gcaagataa 
      tggagataa taagcacgg tacccgttg tgcgggcga 
      agtggctgc tgttgctta caaccaatt ttgcggaat 
      aggcgtcgc ctacaactc gagcatagg aggtgtacg 
      aatgctgga cggcgtggt aaacgacgc cgtcgaggc 
      ccgcgccct gggcctcaa ccccgaaca catcgacat 
      ttacagtgc ttcctgggg ccccgaaga cgacggcaa 
      aaccgtcga cggccctgg acctttagc ccgtcgtgc 
      cttcattta tggcgtgac cagcggcag gaaaggcaa 
      aggttcgat tttcgtttg ggcgtctgg aaacggtgg 
      aagacacac agattcgtg caattgcga cggctacac 
      caacagtat tttcacttt gagcatctc tagtgcgac 
      ccaaggagg ctacaaacc gtggtattt ggaggagtg 
      ttcctcgac gcttgccac aacgtacag ttccggaac 
      tccaggtca tgataaaag cgtcgcgac ggtggatat 
      ggacgcgag acttcgccc cgatcatat ttgcacagt 
      tgaacatac ggggacttc ggcttcggc tccgttagc 
      ggccggaat ttgtgccct agcgcttga ggcaaatcc 
      gagcttgac ttggaggga tatgcaata tttggtggt 
      tttgacctc acggtcggc ccccttgga gaaggaaag 
      tggctggat tacgaatgg ggttggccg gaaagtgtc 
      gcataaatt tggatatgg acttatgga tgctggagc 
      tttggtaag tcttgccga gaaatggac tacggtacc 
      accgcagca catctgcaa atcacaaga actggtcga 
      agatcggca aattgacgc tgcgtatgg tacgacttt 
      ggacttcca tatggacgt ggatgcttg tagtaactc 
      actgaacga agtccattt tctggaaca cgtccaatg 
      taagatttc gttgcggtt cttcccgcg tggcaattt 
      gcgcattat tttgatttc tccaatggg tacgacttc 
      aacgttgtt gtttgaaag gccacgaga cgtcgtcag 
      ttcgaattt cgacgattg gccgttctt aagtgttca 
      tttctgggg cgaaagagc tgaaggccg gtggacttt 
      acgtattgt aaatgctgg taatcgaca cgtgaacca 
      accgggagt tttgaaaaa atggcaatt gatttttta 
      cggcacttc tgttaatcc catacgagt aagacctgc 
      aggcaggcc tcaatctag tccctggaa gtcgccgct 
      taatacttt tacatttcc tactgtctc cgaatcggt 
      gactcaagt caccgataa tttcttcaa agattttca 
      caacttccc caatcttta ttcgtttgc tggatccga 
      gcccgaaca agccgtcag ttcgttgga cgggaaaaa 
      gaccaaaat tcgagacaa cgataacaa tattgacga 
      cgaagatga agaaactaa agaaaactg tgatgaaca 
      atgtgatga tcaaggttg tttcggccg gggaccgac 
      taaatgtat agcttgtag aaataaacg gatggatag 
      gacttgtgt cagcacttg tccacctcg cagttacgc 
      cgattccca gggtgtatg tcagccgtg tcatgaagc 
      ttgcgagac ttgcaccgg atcggacca aaaaagttg 
      ccttacgtg ttcaccagg tcacgtgag agtcgtcga 
      tctggatat ttgtctaca gcaatgtcc cgaaggcta 
      tttccaaga tttcatcgc aaagacttg caccccttg 
      ccaagccaa ctgcgccaa ttgtcaaga ccggcccga 
      ccattgcac cagttgcga ccaccacct ccttctcta 
      ccagaacaa atgcctcgc gttttgtcc tcccaatac 
      gtacgaaac cgaagatta cacgtgtgc cccttgtca 
      cgaaacttg cgagacttg caacggttc gaatagttc 
      tcagtgtgt ttcctgcac acaaggacg cttctggca 
      cgaaaatcg ttgcgtgaa ggaatgccc cgctcatca 
      ctacgccga cccacatca acacgagtg tatagaatg 
      ccctccagg gtgttcgga atgcaacaa aacgacctg 
      catttcgtg tctggacga ttggcaagt gaacgcgaa 
      gggccgatg cgtgcccca cggaagcga acgctgtga 
      tatagacca atatttcga cggcggcct gtgcaagcc 
      ctgccactc gacttgcga atcctgcga cggccccac 
      cgaacacgc ctgtctttc ctgcgccag ccctctcat 
      ccttcaaga cactcgttg cgtcgccgt ctgtgataa 
      aaccttctt tcacgataa aggcacccg tctgtgtga 
      accctgtct ccatacttg cactaaatg cgtttcgaa 
      aatgaactg tagcgagtg catacccgg attacactt 
      gcaaagtgg cgaatgcag ttccacgtg tgccacagg 
      ctattacag cgataaagg aatctgtgc gagatgtta 
      catgagttg taaaacgtg tagcggtcc ggcacacaa 
      tcagtgtgt tagttgtcc gcaaggttg gcaactttt 
      ggccgggga atgccggcc tgattgtcc agaggggta 
      ttacaagac ggaatatgg gtgtcaaaa gtgccatta 
      ttcatgcag gaattgcat agggtgtaa ggtgcaaga 
      agacaaacg aaggattga cgtacaagc tttattgct 
      ttcagcaca acccgtatc gactttaca attgatcgg 
      cccgtttaa tttcattac gggcctggc aataatcgc 
      cgttttatg tgtagtaat cctttttat aacagtttt 
      tatagttct acagagaaa cagtattca ggcagcgcc 
      gagaggcta cagcagggt atccataaa aaaacctaa 
      agttacatg taattacaa tgtggtggc tgtgagtga 
      tggcgattc gtcggattc ggacagttc agaaggtca 
      caatttagt aacaaagcc gaaagctga tcggactgc 
      ttgttaaaa attcttaaa ataagcgag tgtgtaaat 
      aattctgtc ttcattggc ctgtgttgt gttttataa 
      gcgtcaagg ttgtaatgt atatattgt gagaaccct 
      caatgaaac gaaatgatt accacttgt accaaaatc 
      ctatgtgtt attttataa gatgtttta atatcaatg 
      aagacagct caatgtaaa ttttaagat gcgaaactt 
      aagtgctct tgatatttc atgtcgtaa atatagtta 
      tttttaaat gtacacttt gtatataat caatagata 
      attgtttca atgttattt atgaaattt accatccaa 
      ccaatccag ttttggttt tatagtttg tctcagtta 
      ataaaaaat ttatatttt t
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