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Gene information
General information
TC004550 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2691770..2694126
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004550
    iBeetle screen iB_03782

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004550 RA
      MVSESERTF LGLDLSTQQ LKAAVVNNS LQIIHEASV 
      MFDSDLPEF RTHGGVQID KTNPSVITA PTIMWVKAL 
      DMLLDKLTV AGVDFSKIA AISGSAQQH GSVYWQKGS 
      EKLLSNLNP SEFLHQQLA SSFTIANSP VWMDSSTSK 
      QCKELEDAV GGPQKLAEI TGSRGYERF TGPQIAKIK 
      QTRPEAYKN SERISLVSS FLCSLFLGK IAPIDFTDG 
      SGMNLMDIK TKKWHADLL QVIGPDLEE KLGGLVASN 
      ANVGPISPY FVERYSFDP NCRIIACTG DNPASLVGM 
      RLNEGWLAV SLGTSDVLF VWLEEPKIV LNGHILCNP 
      VDSNAYMAM LGFKNGSLT RERIRNACA ESNWDIFNQ 
      LLDSTPRGN FGNMGLYYD VQEILPFLS GDYRFNKAN 
      ERVTRFTSL EVEVRALIE GQFIARRAY VEDIGFKLD 
      KETKILATG GASNNKAIL QVLSDVFNA PVYLLDESK 
      SAMLGAAYQ AKHGLLGSQ SNYFEITSC LPPPRLICQ 
      PYSDASEIY EPMVARYRK LEAMLLGNK V
    CDS sequence
    • >TC004550 RA
      atggtgtcc gagtccgaa cggactttc ctgggtcta 
      gatttaagt acccaacag ctcaaagcc gctgttgtc 
      aacaacagc ctccaaatt atccatgaa gcttccgtc 
      atgttcgac agcgattta ccagaattt cgcacacat 
      ggtggtgtc caaatcgac aaaaccaac cctagtgtt 
      atcacagca cccaccata atgtgggtt aaggcctta 
      gacatgctt ctggataaa ctaacagtc gctggagtc 
      gatttttcg aaaatcgct gccatttca ggctccgcg 
      caacaacac ggttcggtc tattggcaa aaaggctcc 
      gaaaaactc ctctcaaat ttaaacccg agcgaattt 
      ttgcaccaa caattagcc tcaagcttc acaattgca 
      aacagccca gtctggatg gattccagt acgagcaaa 
      cagtgcaaa gagctcgaa gatgcagtc ggagggcct 
      caaaaactt gccgaaatt acgggctct cggggctac 
      gaaagattc actgggccc caaattgcc aaaataaag 
      caaacgagg cccgaggct tacaaaaac agcgaacga 
      atttcccta gttagtagc ttcctgtgt tcacttttc 
      ttgggcaaa atcgcccca attgatttt acggacggc 
      tcagggatg aatctcatg gatattaaa acaaaaaaa 
      tggcatgcg gacttgttg caagtgatt ggacctgat 
      ttagaagaa aaattaggt ggtttagta gcatcgaac 
      gccaatgtt ggtccaatc tcgccgtat tttgttgaa 
      aggtatagt tttgacccg aattgtcgg attattgcg 
      tgtactggg gacaatccc gcgtcgctt gtcggtatg 
      agactaaat gaaggctgg ttagctgtg agtttgggc 
      acaagtgat gtgcttttt gtatggttg gaagagccc 
      aaaattgtg ctaaacggc cacattttg tgtaatcca 
      gtcgattcg aatgcctat atggctatg ctgggtttt 
      aaaaacggg tctttgacg cgagaaaga atcagaaat 
      gcgtgtgct gagtcaaat tgggacatt ttcaaccaa 
      ttattagac agtacacca cgtggaaat ttcggaaat 
      atgggattg tactacgac gtccaggag atcctaccg 
      tttttgtcc ggcgattac aggtttaat aaagccaat 
      gaacgagtg acaagattt acaagtttg gaagtcgaa 
      gtccgggct ttgattgaa ggacagttt atcgcaaga 
      cgggcttac gtcgaagac attggattt aaattggac 
      aaagaaacg aagattcta gcaacggga ggtgcttcc 
      aataataaa gcaattctt caagtgctt tcggacgtc 
      ttcaacgca cctgtttac ctcctagac gaatcgaag 
      tcggccatg ttgggggcc gcttatcaa gcgaaacac 
      ggacttttg ggctctcaa agtaactac tttgagatt 
      acgtcatgt ctcccccca ccacgactc atttgtcaa 
      ccatattcc gacgctagt gagatctac gaaccaatg 
      gttgcgcga tatagaaag ttggaggct atgctttta 
      ggaaataaa gtctga
    mRNA sequence
    • >TC004550 RA
      cctcgaagt caagctaag ccgtgggtt actttacag 
      taccagatt tgtctttcg ttggtgcaa aatcgtggt 
      aaaaatggt gtccgagtc cgaacggac tttcctggg 
      tctagattt aagtaccca acagctcaa agccgctgt 
      tgtcaacaa cagcctcca aattatcca tgaagcttc 
      cgtcatgtt cgacagcga tttaccaga atttcgcac 
      acatggtgg tgtccaaat cgacaaaac caaccctag 
      tgttatcac agcacccac cataatgtg ggttaaggc 
      cttagacat gcttctgga taaactaac agtcgctgg 
      agtcgattt ttcgaaaat cgctgccat ttcaggctc 
      cgcgcaaca acacggttc ggtctattg gcaaaaagg 
      ctccgaaaa actcctctc aaatttaaa cccgagcga 
      atttttgca ccaacaatt agcctcaag cttcacaat 
      tgcaaacag cccagtctg gatggattc cagtacgag 
      caaacagtg caaagagct cgaagatgc agtcggagg 
      gcctcaaaa acttgccga aattacggg ctctcgggg 
      ctacgaaag attcactgg gccccaaat tgccaaaat 
      aaagcaaac gaggcccga ggcttacaa aaacagcga 
      acgaatttc cctagttag tagcttcct gtgttcact 
      tttcttggg caaaatcgc cccaattga ttttacgga 
      cggctcagg gatgaatct catggatat taaaacaaa 
      aaaatggca tgcggactt gttgcaagt gattggacc 
      tgatttaga agaaaaatt aggtggttt agtagcatc 
      gaacgccaa tgttggtcc aatctcgcc gtattttgt 
      tgaaaggta tagttttga cccgaattg tcggattat 
      tgcgtgtac tggggacaa tcccgcgtc gcttgtcgg 
      tatgagact aaatgaagg ctggttagc tgtgagttt 
      gggcacaag tgatgtgct ttttgtatg gttggaaga 
      gcccaaaat tgtgctaaa cggccacat tttgtgtaa 
      tccagtcga ttcgaatgc ctatatggc tatgctggg 
      ttttaaaaa cgggtcttt gacgcgaga aagaatcag 
      aaatgcgtg tgctgagtc aaattggga cattttcaa 
      ccaattatt agacagtac accacgtgg aaatttcgg 
      aaatatggg attgtacta cgacgtcca ggagatcct 
      accgttttt gtccggcga ttacaggtt taataaagc 
      caatgaacg agtgacaag atttacaag tttggaagt 
      cgaagtccg ggctttgat tgaaggaca gtttatcgc 
      aagacgggc ttacgtcga agacattgg atttaaatt 
      ggacaaaga aacgaagat tctagcaac gggaggtgc 
      ttccaataa taaagcaat tcttcaagt gctttcgga 
      cgtcttcaa cgcacctgt ttacctcct agacgaatc 
      gaagtcggc catgttggg ggccgctta tcaagcgaa 
      acacggact tttgggctc tcaaagtaa ctactttga 
      gattacgtc atgtctccc cccaccacg actcatttg 
      tcaaccata ttccgacgc tagtgagat ctacgaacc 
      aatggttgc gcgatatag aaagttgga ggctatgct 
      tttaggaaa taaagtctg aatatacgt aataaatat 
      accattcta gtgata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY