Status
Please wait ...
Gene information
General information
TC031955
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004534,TC004533
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3043143..3048907
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031955
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03780

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (13 dpi)eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (14 dpi)
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Adult morphology
    prothoracic odoriferous glands darker - Number of animals showing the phenotype: 5 (41 dpi)
    Days post injection



    abdominal odoriferous glands darker - Number of animals showing the phenotype: 2 (41 dpi)
    Days post injection



    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031955 RA
      MPLDESKDT LGGKKIRFK TPARVLFTS ESKIDDLLR 
      NDKEAQEGE AKKERSPSL ASFLQVELT RDYLLEHDE 
      ERFSARREK VYSFMRIPR EVERFMRYG FMQCTDSFL 
      FVYTFLPMR VLLALWALV TRPFSQCFG VHDRGRRVL 
      TPAEICDLL KAVILVTCS MIMFSVDTN MLYHLIKSQ 
      SVIKLYIFY NMLEVGDRL FSAFGQDTI DALFWTATE 
      PRGRKREHL GVIPHLIFA IVYVILHSI LVLFQATTL 
      NVAINSNNK ALLTIMMSN NFVELKGSV FKKFDKNNL 
      FQVSCSDVR ERFHLIVLL SIVVLQTMR EYSWKWDRL 
      WVLVPDCLI VLIAEMFVD WIKHAFITR FNELQLDVY 
      RDYTTSLAY DMAQTRQKH AFSDHSDLV ARRMGFIPL 
      PLGVVMIRV LVSAIHIND FASVILFIL AYFCLGSFK 
      VLNSLLILG KACDLIAQH KQEKATVQS PNSKTTTTA 
      TSPLRPPVV DQPVRYSNG FPPVEIKAD LGPAAIFAN 
      STVDLKNVS LNEELLKVE GEDIKIEPA LEQGEIVTR 
      SFPDINSKE LEDIGGDGL KRAESEPSL NICSDESDL 
      PK
    CDS sequence
    • >TC031955 RA
      atgcctttg gacgaaagt aaagacaca ctagggggt 
      aaaaaaatc cgattcaag acccctgcg cgtgttttg 
      ttcacttcc gagagtaaa atcgacgac ttattgcga 
      aatgacaag gaggcgcaa gaaggggag gcaaagaaa 
      gagagaagt ccatccctg gcatcgttc ttacaagtg 
      gagttgacc cgtgactac ctcctggaa cacgacgag 
      gagcgtttt tcagctcgg agggaaaaa gtgtactcc 
      ttcatgcgt atcccccga gaagtggaa cgtttcatg 
      cgatatggt tttatgcaa tgcaccgat tcgtttctg 
      tttgtttac acgttttta cctatgaga gttttatta 
      gcactttgg gctctggtg actcgaccg ttttcacag 
      tgttttggg gtacatgac agggggcga cgggtttta 
      accccggcg gaaatttgt gatttattg aaggctgtt 
      attcttgtt acgtgtagt atgattatg ttttcggtt 
      gatacgaat atgttgtat catttgata aagagtcag 
      tcagtgatt aagttgtac attttttac aatatgttg 
      gaagtaggg gatcggttg ttttcggcg tttggacag 
      gacaccatt gatgcgctg ttttggaca gcgacggag 
      ccccgaggg cgaaaacgg gagcacttg ggcgttatc 
      ccgcacctg atttttgcc atcgtctat gtcatactt 
      cacagcatt ttggttctg tttcaagcc acgactctc 
      aacgtggcc ataaactcc aacaacaag gcgcttttg 
      accatcatg atgtcaaat aacttcgtc gagctgaaa 
      ggtagcgtt ttcaagaaa ttcgacaaa aacaacttg 
      tttcaagtg tcttgcagc gacgtgagg gagcgtttc 
      cacctaatc gtcctcctt tcaatcgtc gtattgcaa 
      acgatgcgc gaatattcg tggaaatgg gaccgactc 
      tgggtgcta gtccccgac tgtcttatc gttttgatc 
      gctgagatg ttcgtcgat tggataaag cacgcgttt 
      atcacccga tttaacgaa ttacagctt gatgtttat 
      cgcgattat acaactagt ttggcgtat gacatggcg 
      caaacccgc cagaaacac gcgttttcc gaccattcg 
      gacctagtg gcgcgccgg atgggcttc atcccgctg 
      ccactgggc gttgtgatg atccgagtg ctggtcagt 
      gcgatccat atcaacgac ttcgcctcg gttattttg 
      ttcattttg gcgtacttc tgtctcggg tcgttcaaa 
      gtcttgaat agtttgctg attttgggc aaagcttgc 
      gatttgata gcgcagcac aagcaggag aaggcgacc 
      gttcagtcc ccgaattcg aagacgacg accactgcg 
      acgagtccc ttgcggccg ccggttgtg gaccaaccc 
      gtgaggtac agcaatggg tttccccca gttgaaatc 
      aaggcagat ttgggccca gcggctatt tttgccaat 
      agtaccgtt gatttgaaa aacgttagt ttgaatgaa 
      gagttgttg aaggtggaa ggtgaagat attaagatt 
      gagccggcg ctcgaacaa ggcgaaatt gtgacgagg 
      agttttccg gatattaat agtaaggag ttggaggat 
      atcggaggg gatgggttg aaaagggcc gagtcggag 
      ccgtcgttg aatatatgc agtgacgag agtgatttg 
      ccaaagtag
    mRNA sequence
    • >TC031955 RA
      tcgtgttta tgtgtcaat gtcagtgcg tggaatcat 
      gtcgtaatt tttgttttt tcccctaat tatgccttt 
      ggacgaaag taaagacac actaggggg taaaaaaat 
      ccgattcaa gacccctgc gcgtgtttt gttcacttc 
      cgagagtaa aatcgacga cttattgcg aaatgacaa 
      ggaggcgca agaagggga ggcaaagaa agagagaag 
      tccatccct ggcatcgtt cttacaagt ggagttgac 
      ccgtgacta cctcctgga acacgacga ggagcgttt 
      ttcagctcg gagggaaaa agtgtactc cttcatgcg 
      tatcccccg agaagtgga acgtttcat gcgatatgg 
      ttttatgca atgcaccga ttcgtttct gtttgttta 
      cacgttttt acctatgag agttttatt agcactttg 
      ggctctggt gactcgacc gttttcaca gtgttttgg 
      ggtacatga cagggggcg acgggtttt aaccccggc 
      ggaaatttg tgatttatt gaaggctgt tattcttgt 
      tacgtgtag tatgattat gttttcggt tgatacgaa 
      tatgttgta tcatttgat aaagagtca gtcagtgat 
      taagttgta catttttta caatatgtt ggaagtagg 
      ggatcggtt gttttcggc gtttggaca ggacaccat 
      tgatgcgct gttttggac agcgacgga gccccgagg 
      gcgaaaacg ggagcactt gggcgttat cccgcacct 
      gatttttgc catcgtcta tgtcatact tcacagcat 
      tttggttct gtttcaagc cacgactct caacgtggc 
      cataaactc caacaacaa ggcgctttt gaccatcat 
      gatgtcaaa taacttcgt cgagctgaa aggtagcgt 
      tttcaagaa attcgacaa aaacaactt gtttcaagt 
      gtcttgcag cgacgtgag ggagcgttt ccacctaat 
      cgtcctcct ttcaatcgt cgtattgca aacgatgcg 
      cgaatattc gtggaaatg ggaccgact ctgggtgct 
      agtccccga ctgtcttat cgttttgat cgctgagat 
      gttcgtcga ttggataaa gcacgcgtt tatcacccg 
      atttaacga attacagct tgatgttta tcgcgatta 
      tacaactag tttggcgta tgacatggc gcaaacccg 
      ccagaaaca cgcgttttc cgaccattc ggacctagt 
      ggcgcgccg gatgggctt catcccgct gccactggg 
      cgttgtgat gatccgagt gctggtcag tgcgatcca 
      tatcaacga cttcgcctc ggttatttt gttcatttt 
      ggcgtactt ctgtctcgg gtcgttcaa agtcttgaa 
      tagtttgct gattttggg caaagcttg cgatttgat 
      agcgcagca caagcagga gaaggcgac cgttcagtc 
      cccgaattc gaagacgac gaccactgc gacgagtcc 
      cttgcggcc gccggttgt ggaccaacc cgtgaggta 
      cagcaatgg gtttccccc agttgaaat caaggcaga 
      tttgggccc agcggctat ttttgccaa tagtaccgt 
      tgatttgaa aaacgttag tttgaatga agagttgtt 
      gaaggtgga aggtgaaga tattaagat tgagccggc 
      gctcgaaca aggcgaaat tgtgacgag gagttttcc 
      ggatattaa tagtaagga gttggagga tatcggagg 
      ggatgggtt gaaaagggc cgagtcgga gccgtcgtt 
      gaatatatg cagtgacga gagtgattt gccaaagta 
      gctgaaact agtcgttcc ttgttttta tttgaattc 
      catccgttt tttttttct attttgttt aagtccaaa 
      gactttgag tgttgatat tttcatatt gcagtttat 
      aaataaata tttttatat gag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY