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Gene information
General information
TC004530 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 1001215..1034335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004530
    iBeetle screen iB_03779
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    procephalic head partiallynot present - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna unilateralnot present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004530 RA
      MATIDSGTE TLFSDDSSC ELLGSPSVC YGDEGQFED 
      NCSEVNRLA PPNIQPFSG VLEKGKVVI RPIAFKPAA 
      MTPAARFGL AGERYGSTP ILTRPGSRL TLYGSTNDL 
      RQPTATSNY SLDRKLRSS CPSASSSSP PLAMSSLTS 
      LPHKLVNYD SLESVRKSP VSTADGSMM NKASYRLSG 
      AGGGSLLDL TPSPSDSGV SELEAALRD RDSELAYLR 
      QTMEHNEQV IFRVYQEKE KVWERELRR LKTVHENRL 
      RASAQKALK LEQMLMMQT YQLQQDKKR LCAETQRAN 
      CQSERLKQE VNALRGRLE ETEWGLCQK TGEISLLKS 
      QLKDYQNEQ TAKCQELLQ LRTEHRDFR EQLDLREAE 
      ISELRHQSE EKDAEIKLL KEQLARLGN KSPTIDDLK 
      EEIRELREE LSDMSLSEY GSVEPGRSQ RISQEDLEI 
      ECSCSSLAK LKQEIASKT KDFENERVV WAQEKEKVL 
      RYQRQLQMN YVQMYRRTR ALEAEVESL TIELELDKT 
      GKKQLPGGD LTQTIEL
    CDS sequence
    • >TC004530 RA
      atggccacg attgatagt gggacggag acgctcttt 
      tccgacgac tcctcgtgt gagctgttg ggctcaccg 
      agcgtttgc tatggggac gaaggacaa ttcgaggat 
      aactgttcg gaggtcaac agacttgcg cctccgaac 
      atacagccg tttagtggc gttttggaa aagggtaag 
      gtagtgatt cgaccgatc gcttttaaa cctgcagcc 
      atgacacct gcggcaaga ttcggttta gcaggagaa 
      cgatacgga tcaacgcct atcctcaca agacccggt 
      tcgcgcctg actctgtat ggaagcacc aatgatttg 
      cgacagccc accgcaact tcaaactat tccctcgac 
      cgaaaattg cgctcctcg tgtccttcg gcctcctcc 
      tcttcgccg ccgcttgcc atgagctcc ctgacgtcg 
      cttccgcat aagctcgtc aattacgac agcctggaa 
      agcgtgagg aaatcgccc gtttcgacg gccgatggc 
      tcgatgatg aacaaagcg tcgtatcgt ctatcgggc 
      gcgggcggc ggaagtctc ctcgacctg acgccgtct 
      ccatccgac tcaggagtg tccgaactg gaggcggcg 
      ctgcgcgac agagactcc gaactcgcc tacctccgc 
      caaacgatg gagcacaac gagcaagtc attttccgc 
      gtctaccaa gagaaggaa aaggtctgg gagcgcgaa 
      ctcagaaga ctcaaaacg gtccacgaa aacagactg 
      agagccagc gctcagaag gcgctcaaa ctcgagcag 
      atgctcatg atgcagacc taccagctg caacaggac 
      aagaagcgg ctctgtgcc gagacacaa agggccaat 
      tgccaaagc gagaggctc aagcaggag gtgaacgcg 
      ctcaggggg cgtctggag gagaccgaa tgggggctc 
      tgccaaaaa accggcgaa atttcactc ttaaagtca 
      cagttgaag gattatcaa aacgagcaa accgccaaa 
      tgccaggag ctcctccaa ctccgcaca gaacaccgc 
      gacttccgc gaacaactg gacctgcgt gaggcggaa 
      atctccgaa ttgcgacac cagtcagag gaaaaagac 
      gccgaaatt aagcttctg aaggaacag cttgcaagg 
      ttggggaac aaatcgcca actatcgat gatctcaag 
      gaagaaatt cgagagttg cgtgaggag ctctctgac 
      atgtccctc agcgagtat ggaagtgtc gaacccgga 
      agatcgcag cggatatcg caggaagac ctcgaaatc 
      gagtgttcc tgctcgagt ttagccaaa ctgaagcaa 
      gaaattgcc tccaaaacg aaagatttt gaaaacgaa 
      agggtggtt tgggcgcag gaaaaggag aaagtgttg 
      cggtaccag aggcaactg cagatgaat tatgtgcag 
      atgtatcgg agaacgcga gctttggaa gccgaagtt 
      gagagtttg acgattgaa ctcgagttg gacaaaact 
      gggaagaag caactacct ggcggtgat ttgacacaa 
      actatcgaa ctgtag
    mRNA sequence
    • >TC004530 RA
      tttgcgacc tgtatccac gctaacgtg gattaccgc 
      ctaaagttt cgaatcaat tttttaagt gtaaatatg 
      tgaattatg gccacgatt gatagtggg acggagacg 
      ctcttttcc gacgactcc tcgtgtgag ctgttgggc 
      tcaccgagc gtttgctat ggggacgaa ggacaattc 
      gaggataac tgttcggag gtcaacaga cttgcgcct 
      ccgaacata cagccgttt agtggcgtt ttggaaaag 
      ggtaaggta gtgattcga ccgatcgct tttaaacct 
      gcagccatg acacctgcg gcaagattc ggtttagca 
      ggagaacga tacggatca acgcctatc ctcacaaga 
      cccggttcg cgcctgact ctgtatgga agcaccaat 
      gatttgcga cagcccacc gcaacttca aactattcc 
      ctcgaccga aaattgcgc tcctcgtgt ccttcggcc 
      tcctcctct tcgccgccg cttgccatg agctccctg 
      acgtcgctt ccgcataag ctcgtcaat tacgacagc 
      ctggaaagc gtgaggaaa tcgcccgtt tcgacggcc 
      gatggctcg atgatgaac aaagcgtcg tatcgtcta 
      tcgggcgcg ggcggcgga agtctcctc gacctgacg 
      ccgtctcca tccgactca ggagtgtcc gaactggag 
      gcggcgctg cgcgacaga gactccgaa ctcgcctac 
      ctccgccaa acgatggag cacaacgag caagtcatt 
      ttccgcgtc taccaagag aaggaaaag gtctgggag 
      cgcgaactc agaagactc aaaacggtc cacgaaaac 
      agactgaga gccagcgct cagaaggcg ctcaaactc 
      gagcagatg ctcatgatg cagacctac cagctgcaa 
      caggacaag aagcggctc tgtgccgag acacaaagg 
      gccaattgc caaagcgag aggctcaag caggaggtg 
      aacgcgctc agggggcgt ctggaggag accgaatgg 
      gggctctgc caaaaaacc ggcgaaatt tcactctta 
      aagtcacag ttgaaggat tatcaaaac gagcaaacc 
      gccaaatgc caggagctc ctccaactc cgcacagaa 
      caccgcgac ttccgcgaa caactggac ctgcgtgag 
      gcggaaatc tccgaattg cgacaccag tcagaggaa 
      aaagacgcc gaaattaag cttctgaag gaacagctt 
      gcaaggttg gggaacaaa tcgccaact atcgatgat 
      ctcaaggaa gaaattcga gagttgcgt gaggagctc 
      tctgacatg tccctcagc gagtatgga agtgtcgaa 
      cccggaaga tcgcagcgg atatcgcag gaagacctc 
      gaaatcgag tgttcctgc tcgagttta gccaaactg 
      aagcaagaa attgcctcc aaaacgaaa gattttgaa 
      aacgaaagg gtggtttgg gcgcaggaa aaggagaaa 
      gtgttgcgg taccagagg caactgcag atgaattat 
      gtgcagatg tatcggaga acgcgagct ttggaagcc 
      gaagttgag agtttgacg attgaactc gagttggac 
      aaaactggg aagaagcaa ctacctggc ggtgatttg 
      acacaaact atcgaactg tagtatttt ttctcgtgt 
      agattgctt tgtctacat tttgagtta aaagtattc 
      aagttatct ttgcagtag aaacatgat tattgtagt 
      tttaaggat gacatgtga ttttctttt tcattattt 
      attacctac tcattgtaa ataagtatt atcactgaa 
      gctatttac gtaattttt accattatt cctataggt 
      tctagtatt taacattgt tacgtgtat atacaaata 
      aaagtgtta tctaaag