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Gene information
General information
TC004527 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3138218..3144668
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004527
      iBeetle screen iB_03778

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
      labium mostlynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: bad quality of preparation,
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004527 RA
        MKYPQLVSM PSAVSDWLE EQLEARGID AVVYTRYIL 
        SLLHSDTVD VIYSDEDLH FTHLKKEVR RPGGSRKRF 
        RRSSDWWKC AQADAEKLK RSAAIECLM SASEQTCGI 
        ESLIDELCD KLNEIKSDD DEHHVDPPV KKPELQQVS 
        PQELAKRYY AAFPPLNRN TTPTLIPKW IPSPKKKPK 
        HSEMKASRT AGKQRFGAY ITSRKKENN EKKPKPSCD 
        EVNLYDDLP VDIKELLDD SSPNSNVEM MNLANMRDT 
        GRRGFISCG TNITSSIWT NEAAMEENY DSVLDLQFS 
        SITINNWFD LPAPSGRWS DDSGCFEDD YLYNQFSLS 
        LVKLNHDKE VSAFAEVVP KNRHNQYNP FSKIIGSGI 
        HRDKKIELE KEEDLLTSV HSHFRPINE KVEGGQYAD 
        GATFIVSNT WDKINYRRT ESGMYMEND YSMRKKYCE 
        YNKKGTNMD FVLKYLVCQ NDKSCQTDD LDAEVEAIN 
        RETCTCRTL LNDCRVHPT CDKCNNNSW NTNLGLKTD 
        LRWQFGTTN IWSGGSVCH DCLGRPPST LQHSKLRED 
        VSQDGDQLL SDLSTIHKT YQQESLPVT EFTCDIAEF 
        FLPKERKRR HSLMAQMED SWSFAERLE EDCLIQMST 
        IPTLRSVTL
      CDS sequence
      • >TC004527 RA
        atgaagtat ccgcaacta gtgtccatg ccctcggcg 
        gtttctgat tggctagag gaacagttg gaagctcgt 
        ggaatagat gctgtagta tacactcgt tacatactt 
        agtctttta cacagcgat actgtagat gtcatttac 
        tcagatgaa gatctacat ttcactcac ttgaaaaag 
        gaagtgaga cgtccgggt ggaagtcgt aagcgtttt 
        cgtcgctcg tccgattgg tggaaatgt gcgcaagcc 
        gacgccgaa aagctcaaa cgttcagca gcgatcgaa 
        tgtctaatg tccgcatct gaacagact tgcggaatt 
        gagtcatta atcgacgag ttatgcgac aaattgaac 
        gaaatcaag agtgacgac gacgagcat cacgtcgat 
        cctcccgtc aagaaacca gagctccag caagtttcc 
        ccccaagaa ttggccaaa cgctattac gccgctttt 
        cctccactc aatcgtaac acgaccccc accttgata 
        cccaagtgg ataccgtct ccgaagaag aagcccaaa 
        cacagcgaa atgaaggcg agcagaacg gccggaaag 
        cagcgcttt ggcgcctac atcaccagt cgcaagaag 
        gagaacaac gagaaaaaa cccaaacca tcgtgtgac 
        gaagtcaat ctctacgac gatctcccg gttgacatc 
        aaggagctg ctggacgat tcctccccg aattcgaac 
        gtcgaaatg atgaatctg gccaacatg cgggatacg 
        ggacgtcgc ggttttatt tcgtgtggt acgaacatc 
        acgagctcg atttggacg aacgaagct gcaatggaa 
        gagaattac gactcagtg cttgatttg caattcagc 
        tcaatcact attaacaat tggttcgat ctgccggcg 
        ccaagtggg cggtggtcg gacgatagt ggttgcttc 
        gaagatgat tacttatac aaccaattc agtctgagt 
        ttagtcaag ttgaaccac gataaagaa gtgagtgct 
        ttcgccgaa gtcgtaccg aaaaatcgc cacaaccag 
        tacaaccct ttttcgaaa atcatcggt tcggggatt 
        caccgagac aaaaaaatc gagctggag aaggaggag 
        gatttgctc acttcggtc cacagtcat tttcggcca 
        attaacgaa aaagtcgaa ggggggcag tacgcagat 
        ggcgccact ttcatcgtt tcaaacacc tgggacaag 
        atcaactac cgccgcacc gaaagtggc atgtatatg 
        gaaaacgac tatagcatg cgcaaaaaa tactgcgag 
        tataacaag aaagggacg aatatggat tttgttttg 
        aaatatctc gtctgtcag aacgataaa agctgtcaa 
        acggatgat cttgatgcg gaagttgaa gcaatcaat 
        cgggagact tgcacttgt cgcacgtta ctaaacgac 
        tgtcgggtg catcccact tgcgacaag tgcaacaac 
        aattcatgg aacacgaat cttggttta aaaacggat 
        ctgcggtgg cagtttggg accactaat atttggagc 
        gggggcagt gtgtgtcat gattgtctg gggcgcccc 
        ccctccacc ctccaacac agcaaactt cgcgaagac 
        gtcagtcag gatggcgac cagttattg tccgatttg 
        agtacaatt cacaaaacc tatcagcag gaaagcctg 
        ccggtgact gaattcacg tgcgacatt gctgaattt 
        ttcctcccg aaagaacgc aaacgccgc cactcgctg 
        atggcccag atggaggat tcgtggagt ttcgccgaa 
        cgtctggag gaggattgt ctaatacag atgtctaca 
        atacccaca ctgcgttcg gtcactttg taa
      mRNA sequence
      • >TC004527 RA
        ccaagtggt tggagggaa attgacgaa ggtttggtt 
        ggttgcagg ttcaccaca atggggggt acaagaact 
        caagtgcag tgaatcaac gaacgaaca aatcagtta 
        ttatttttg atgttagct tggtttgca ttatttttt 
        gatttcgtt atggattcc atcgtcgcc gttttataa 
        atttacgat gcgttacga aaagcggtc gcaagttat 
        tgtacgccc ccaggaaat cgagacgca tacctcacg 
        tgaaccact caactgctg catggctga tcatcatta 
        ctatttttt ataaacata ttatgaagt atccgcaac 
        tagtgtcca tgccctcgg cggtttctg attggctag 
        aggaacagt tggaagctc gtggaatag atgctgtag 
        tatacactc gttacatac ttagtcttt tacacagcg 
        atactgtag atgtcattt actcagatg aagatctac 
        atttcactc acttgaaaa aggaagtga gacgtccgg 
        gtggaagtc gtaagcgtt ttcgtcgct cgtccgatt 
        ggtggaaat gtgcgcaag ccgacgccg aaaagctca 
        aacgttcag cagcgatcg aatgtctaa tgtccgcat 
        ctgaacaga cttgcggaa ttgagtcat taatcgacg 
        agttatgcg acaaattga acgaaatca agagtgacg 
        acgacgagc atcacgtcg atcctcccg tcaagaaac 
        cagagctcc agcaagttt ccccccaag aattggcca 
        aacgctatt acgccgctt ttcctccac tcaatcgta 
        acacgaccc ccaccttga tacccaagt ggataccgt 
        ctccgaaga agaagccca aacacagcg aaatgaagg 
        cgagcagaa cggccggaa agcagcgct ttggcgcct 
        acatcacca gtcgcaaga aggagaaca acgagaaaa 
        aacccaaac catcgtgtg acgaagtca atctctacg 
        acgatctcc cggttgaca tcaaggagc tgctggacg 
        attcctccc cgaattcga acgtcgaaa tgatgaatc 
        tggccaaca tgcgggata cgggacgtc gcggtttta 
        tttcgtgtg gtacgaaca tcacgagct cgatttgga 
        cgaacgaag ctgcaatgg aagagaatt acgactcag 
        tgcttgatt tgcaattca gctcaatca ctattaaca 
        attggttcg atctgccgg cgccaagtg ggcggtggt 
        cggacgata gtggttgct tcgaagatg attacttat 
        acaaccaat tcagtctga gtttagtca agttgaacc 
        acgataaag aagtgagtg ctttcgccg aagtcgtac 
        cgaaaaatc gccacaacc agtacaacc ctttttcga 
        aaatcatcg gttcgggga ttcaccgag acaaaaaaa 
        tcgagctgg agaaggagg aggatttgc tcacttcgg 
        tccacagtc attttcggc caattaacg aaaaagtcg 
        aaggggggc agtacgcag atggcgcca ctttcatcg 
        tttcaaaca cctgggaca agatcaact accgccgca 
        ccgaaagtg gcatgtata tggaaaacg actatagca 
        tgcgcaaaa aatactgcg agtataaca agaaaggga 
        cgaatatgg attttgttt tgaaatatc tcgtctgtc 
        agaacgata aaagctgtc aaacggatg atcttgatg 
        cggaagttg aagcaatca atcgggaga cttgcactt 
        gtcgcacgt tactaaacg actgtcggg tgcatccca 
        cttgcgaca agtgcaaca acaattcat ggaacacga 
        atcttggtt taaaaacgg atctgcggt ggcagtttg 
        ggaccacta atatttgga gcgggggca gtgtgtgtc 
        atgattgtc tggggcgcc ccccctcca ccctccaac 
        acagcaaac ttcgcgaag acgtcagtc aggatggcg 
        accagttat tgtccgatt tgagtacaa ttcacaaaa 
        cctatcagc aggaaagcc tgccggtga ctgaattca 
        cgtgcgaca ttgctgaat ttttcctcc cgaaagaac 
        gcaaacgcc gccactcgc tgatggccc agatggagg 
        attcgtgga gtttcgccg aacgtctgg aggaggatt 
        gtctaatac agatgtcta caataccca cactgcgtt 
        cggtcactt tgtaatttc ggggtaggt caattggaa 
        atctcttga taacacacg gctgatcta gtggcttga 
        gccaccaca aaacgacaa actgccata ttaatttca 
        gtgcagtga gaagcccac aagatggct gaataggtg 
        acgcacgtg tgttatcga ttaaatcaa ttgtgtcga 
        aaccgggca agacttgtt atgttttcg ttaagtttg 
        atttgtttt ggtcttgcg cggttcgtt atgtagggg 
        agagttcac attttagtt caattttct aaccgaacc 
        tacattagc gattttcat tttacttgt tatgtgtgc 
        aaaattaag gaaaaagaa tgacaaagc atactttcc 
        cggaagttg agattgaaa aaattattt tatttttgc 
        aattccggg cgtttcctt tcactacta ttgtaaccc 
        aacaatata gttgaagct gtatgtcta tgtataatg 
        ttagggcat tttagtata gctcttgaa gtgcagcaa 
        tgaacacct gtagttgct cgagacggc gcattttga 
        cccttggtg ccaaaatgt gcgggtctc ccctagttg 
        tatttttcg aaatatgta actattata aatattgga 
        aatgggtat taccattac tggtttata tttagtttg 
        ttttttttt tgtttaatc atttttaca ggttaggga 
        gcgcggcgc aaatttttc aatttagtc attcggagc 
        caaagtcag tagtgtatt aataaatcc cgcccctct 
        ctaatcgcg atatcaata aataatgaa ggaaaaaag 
        aaaaatcgt taggtatct gacgcgttt ttcaactga 
        gcgtccgat tttgttttt tgagaaata ccatcgcat 
        atcggatac aattatatt ttttgaagt gcttcgttt 
        caaaaacta gtgcgagtg tgtgaatgt gtcagttga 
        tcaaaaaaa taataattt tgttgtgct aaaatgtat 
        attcaatcg cacctgatt caatcgtta caaagcaca 
        gcaaagtcc caccgttag ttacagtct tgccaactg 
        aaaaggtgc tgttgaatt ttattgtat ttccatcat 
        tttatcgta tgttgtaat taatattgc aataatttt 
        atataaacg tatcgttgt aactcgttc acttgattc 
        agttttttg ttttgttgt tttcttgtt ttgatttta 
        tgattatac gtaataaag tgaacttgt tatttgaaa 
        aaaagtaca cgtaacgga gtagtgcag taattaaaa 
        ttgtgattt gttttaatt attgtgatg tctccgttt 
        gattttgtt gcattttgg tgattggtg gcgcgattt 
        cggtattta ttttttcaa attgacaaa ttgccaatc 
        actggatgc acctcagat gttagtgtg tagcataac 
        cttttattt tagtgccta ttaaatcta gtataattt 
        gaccttttg ccttatcaa acgttacat gtaaccatc 
        tttgtatgt tgtttacat agaaatttt catcaatgt 
        aacatattc aattgactc aattattaa gaaataatg 
        cagtttgtg gcttaattc atagtttta ttattataa 
        taacgaaag gtaaaatct aaaaaaaat atgaaaaaa 
        taaaaatgt caaataatt ttttgttcc attcgaata 
        ggtcagtgt atcaatact tatgaaact tatcaagga 
        cataactaa taattcatc gtcatcaaa atcataaat 
        taacaaaaa aaaatacaa aagttgggt acttgctag 
        cggtctcct attttttaa agcagcaaa actaaatta 
        agtctggca atgagtttg acaaaactt aataaagca 
        agtacaaag ca
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY