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Gene information
General information
TC004522 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 1198646..1206914
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004522
    iBeetle screen iB_03776
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004522 RA
      MRECISVHI GQAGVQIGN ACWELYCLE HGIQPDGQM 
      PSDKTIGGG DDSFNTFFS ETGAGKHVP RAVFVDLEP 
      TVVDEVRTG TYRQLFHPE QLITGKEDA ANNYARGHY 
      TIGKEIVDV VLDRIRKLA DQCTGLQGF LVFHSFGGG 
      TGSGFTSLL MERLSVDYG KKSKLEFSI YPAPQVSTA 
      VVEPYNSIL TTHTTLEHS DCAFMVDNE AIYDICRRN 
      LDIERPTYT NLNRLIGQI VSSITASLR FDGALNVDL 
      TEFQTNLVP YPRIHFPLA TYAPVISAE KAYHEQLTV 
      AEITNACFE PANQMVKCD PRHGKYMAC CMLYRGDVV 
      PKDVNAAIA TIKTKRTIQ FVDWCPTGF KVGINYQPP 
      TVVPGGDLA KVQRAVCML SNTTAIAEA WARLDHKFD 
      LMYAKRAFV HWYVGEGME EGEFSEARE DLAALEKDY 
      EEVAVDSIE GEGDEGDEY
    CDS sequence
    • >TC004522 RA
      atgagggaa tgcatttcc gtccacatt ggccaagcc 
      ggtgtccag atcggcaac gcctgttgg gaactctac 
      tgtctggaa catgggatt caacccgat ggccaaatg 
      ccctccgac aagacaatc ggaggcgga gacgactct 
      ttcaacacc tttttcagc gaaactggc gccggaaaa 
      cacgtcccg agggccgtt tttgtcgac ttggagcca 
      acagtcgtc gacgaagtc cgaaccggt acataccgc 
      caactcttt catccggaa caactaata accggcaaa 
      gaagatgca gctaataat tacgctcgt ggtcattac 
      accatcgga aaagagatc gtggatgtc gtcttggac 
      cggatacgt aaactcgca gatcaatgc accggattg 
      caaggtttt ctcgttttc cattcgttc ggtggaggc 
      accggctca ggcttcact tccctgttg atggaaaga 
      ttatccgtt gactacggc aaaaaatcg aaactcgaa 
      ttttcgatt taccccgca cctcaagtt tccactgcc 
      gtcgtggaa ccttacaat tcgattttg accactcac 
      accaccttg gagcactcc gattgtgcc ttcatggtc 
      gacaatgaa gccatctac gacatttgt cgccgaaac 
      ttggacatc gaacgcccg acttacacc aacctgaac 
      cgattaatc ggccaaatc gtatcgtca atcaccgct 
      tctttgcgc tttgacggc gctttgaac gttgacttg 
      accgaattc cagaccaac ttggtgcct taccctcgc 
      atccacttc cccttagcc acctacgct ccggtgatc 
      tcggccgaa aaagcctac catgagcaa ctaaccgtc 
      gcggaaatc accaacgcg tgctttgaa cccgccaat 
      caaatggtc aaatgtgac ccacgtcat ggtaaatac 
      atggcttgc tgcatgttg taccgtgga gatgtggtc 
      ccgaaggac gtcaatgcg gcgattgcc accatcaag 
      accaaacgt accattcag tttgttgac tggtgtcca 
      actgggttc aaagttggt atcaactac cagccaccc 
      actgtcgtg ccaggaggt gacttggcc aaggtacaa 
      cgtgctgtt tgcatgttg tccaatacc actgccatc 
      gctgaagct tgggccagg ttggaccat aaattcgac 
      ttgatgtac gccaaacgt gctttcgtc cactggtat 
      gtgggtgaa ggtatggaa gaaggtgaa ttctctgaa 
      gctcgtgag gatttggct gctcttgag aaggactac 
      gaggaggtc gcagtcgat tcgattgaa ggcgaaggg 
      gacgaaggc gacgaatat tga
    mRNA sequence
    • >TC004522 RA
      tttgatcaa cgggtgtcg catcgcgga catcgggtg 
      attgtgtct cttgtctct tcgtaaagc tttcaactc 
      gttttcaaa tccaaaatc atgagggaa tgcatttcc 
      gtccacatt ggccaagcc ggtgtccag atcggcaac 
      gcctgttgg gaactctac tgtctggaa catgggatt 
      caacccgat ggccaaatg ccctccgac aagacaatc 
      ggaggcgga gacgactct ttcaacacc tttttcagc 
      gaaactggc gccggaaaa cacgtcccg agggccgtt 
      tttgtcgac ttggagcca acagtcgtc gacgaagtc 
      cgaaccggt acataccgc caactcttt catccggaa 
      caactaata accggcaaa gaagatgca gctaataat 
      tacgctcgt ggtcattac accatcgga aaagagatc 
      gtggatgtc gtcttggac cggatacgt aaactcgca 
      gatcaatgc accggattg caaggtttt ctcgttttc 
      cattcgttc ggtggaggc accggctca ggcttcact 
      tccctgttg atggaaaga ttatccgtt gactacggc 
      aaaaaatcg aaactcgaa ttttcgatt taccccgca 
      cctcaagtt tccactgcc gtcgtggaa ccttacaat 
      tcgattttg accactcac accaccttg gagcactcc 
      gattgtgcc ttcatggtc gacaatgaa gccatctac 
      gacatttgt cgccgaaac ttggacatc gaacgcccg 
      acttacacc aacctgaac cgattaatc ggccaaatc 
      gtatcgtca atcaccgct tctttgcgc tttgacggc 
      gctttgaac gttgacttg accgaattc cagaccaac 
      ttggtgcct taccctcgc atccacttc cccttagcc 
      acctacgct ccggtgatc tcggccgaa aaagcctac 
      catgagcaa ctaaccgtc gcggaaatc accaacgcg 
      tgctttgaa cccgccaat caaatggtc aaatgtgac 
      ccacgtcat ggtaaatac atggcttgc tgcatgttg 
      taccgtgga gatgtggtc ccgaaggac gtcaatgcg 
      gcgattgcc accatcaag accaaacgt accattcag 
      tttgttgac tggtgtcca actgggttc aaagttggt 
      atcaactac cagccaccc actgtcgtg ccaggaggt 
      gacttggcc aaggtacaa cgtgctgtt tgcatgttg 
      tccaatacc actgccatc gctgaagct tgggccagg 
      ttggaccat aaattcgac ttgatgtac gccaaacgt 
      gctttcgtc cactggtat gtgggtgaa ggtatggaa 
      gaaggtgaa ttctctgaa gctcgtgag gatttggct 
      gctcttgag aaggactac gaggaggtc gcagtcgat 
      tcgattgaa ggcgaaggg gacgaaggc gacgaatat 
      tgaacacac catcaagtg catttcaat ttttactaa 
      tatttatta atgcttttg tagttttgt ataagtcaa 
      tatccgata aaagcactt ttatccaat aaacctttg 
      caataaaa