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Gene information
General information
TC004520 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 1227836..1228995
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004520
    iBeetle screen iB_03775
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004520 RA
      MTTSPTSDV FSDEVDSIS IRDKALLKR SVSKHHNNN 
      FKAGYVPVH EQHLTKTGL RGRKTFAFW TLVALLFIL 
      AVGNLLLTV TILGVLRLG QGMESIELV PDEYAVKFF 
      GVTDLGHMY KRDGKIEGF KDEPVAITS EESAVLLNI 
      MSLRNGRPS NQMRVTKNG TSFWGFDSF HVRNKQGAV 
      LFSTDSPNF HALRGANDF DAKIVFTNR IASPVHSKL 
      KVEGRVLTF KGSEGTRMD GKDIFWSAD QDIYLKTNN 
      GSIVLSGSD GTLIDVRRI PIATVKNNN YVTSQYKVC 
      VCMPEGKLF RIPVPSGPN PRVFCHHIN TQPPHNPCM
    CDS sequence
    • >TC004520 RA
      atgactacg tccccaacg tctgatgta ttctcagac 
      gaagtcgac tcaatctca atccgcgac aaagccctc 
      ctcaaacgt tctgtctca aaacaccac aacaacaac 
      ttcaaagcg ggctacgtc ccagttcac gagcaacac 
      ctcacaaaa acgggtctc agaggccgc aaaacgttc 
      gcattttgg actctagtc gccttactt ttcatccta 
      gcggtcggt aatttgcta cttaccgtc acgatttta 
      ggggttttg agactgggc caagggatg gaaagcatc 
      gagttggtc cccgacgaa tacgcggtg aaattcttc 
      ggagtcacg gatttggga cacatgtat aaacgcgat 
      ggtaaaatt gagggtttc aaagacgaa ccggtggca 
      atcacaagt gaggaaagt gccgtttta ttgaacatc 
      atgtctttg cgaaacggc cgacccagc aaccagatg 
      cgtgtcacc aagaacggg accagtttc tgggggttt 
      gactcgttt catgtgaga aataaacaa ggggcggtc 
      ttgtttagc acagacagc ccgaatttc catgcactg 
      cgaggggcc aacgatttt gacgcgaaa attgttttc 
      actaatcgc attgcgagt cctgtgcat agtaaactc 
      aaagtggaa ggtcgcgtg ttgaccttt aaaggttca 
      gaagggacg agaatggat gggaaggat attttctgg 
      agcgccgat caagacatt tatttgaaa acaaataac 
      ggatcgatt gttctcagc ggaagcgat gggacgctt 
      atcgatgtc aggcggatc ccgattgcg acagtcaag 
      aataataat tacgttact tctcagtat aaagtctgc 
      gtttgcatg cctgaaggg aaactgttc aggattccg 
      gtgcccagt ggaccaaat ccgagggtt ttctgccac 
      cacatcaac acccaaccc ccgcataat ccatgcatg 
      taa
    mRNA sequence
    • >TC004520 RA
      cagcactct cgatcgagc gtcggctat ccacaaaca 
      acatgacta cgtccccaa cgtctgatg tattctcag 
      acgaagtcg actcaatct caatccgcg acaaagccc 
      tcctcaaac gttctgtct caaaacacc acaacaaca 
      acttcaaag cgggctacg tcccagttc acgagcaac 
      acctcacaa aaacgggtc tcagaggcc gcaaaacgt 
      tcgcatttt ggactctag tcgccttac ttttcatcc 
      tagcggtcg gtaatttgc tacttaccg tcacgattt 
      taggggttt tgagactgg gccaaggga tggaaagca 
      tcgagttgg tccccgacg aatacgcgg tgaaattct 
      tcggagtca cggatttgg gacacatgt ataaacgcg 
      atggtaaaa ttgagggtt tcaaagacg aaccggtgg 
      caatcacaa gtgaggaaa gtgccgttt tattgaaca 
      tcatgtctt tgcgaaacg gccgaccca gcaaccaga 
      tgcgtgtca ccaagaacg ggaccagtt tctgggggt 
      ttgactcgt ttcatgtga gaaataaac aaggggcgg 
      tcttgttta gcacagaca gcccgaatt tccatgcac 
      tgcgagggg ccaacgatt ttgacgcga aaattgttt 
      tcactaatc gcattgcga gtcctgtgc atagtaaac 
      tcaaagtgg aaggtcgcg tgttgacct ttaaaggtt 
      cagaaggga cgagaatgg atgggaagg atattttct 
      ggagcgccg atcaagaca tttatttga aaacaaata 
      acggatcga ttgttctca gcggaagcg atgggacgc 
      ttatcgatg tcaggcgga tcccgattg cgacagtca 
      agaataata attacgtta cttctcagt ataaagtct 
      gcgtttgca tgcctgaag ggaaactgt tcaggattc 
      cggtgccca gtggaccaa atccgaggg ttttctgcc 
      accacatca acacccaac ccccgcata atccatgca 
      tgtaattgt agcaattta taatacatc gttatcagt 
      gttgccagt cacgaaata aatacgcca gtttttaaa 
      aaactacta attatgtca ataaatgcg tctctggat 
      g