General information | |||
TC031986
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 3324381..3349238 | |
Sequence information | |||
Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
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TC031986 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03774 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)ovary without detectable defects - Number of animals showing the phenotype: 3
larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
cuticle remnants cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
larva cuticle partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larva constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax partially posteriornot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen anterior not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (16 dpi)
- >TC031986 RA
MGEGNGLIE HDLHGSLVP MNGSHSGSS GRSTPNNSN DPAPGKLFV GGLSWQTSS EKLREYFGM FGNVTDVLI MKDPVTQRS RGFGFITFS EPSSVDNVL KVPIHTLDG KKIDPKHAT PKNRPKQPN KTKKIFVGG VSQDTSADE VKAYFSQFG KVEETVMLM DQQTKRHRG FGFVTFENE DVVDRVCEI HFHTIKNKK VECKKAQPK EAVQASAQL LGKRLMLSG LGMRMATPT AGAIAAATN PLAVVQAQA HVQAAAVAA AAQAQTAAV ASYGKLFGA YPPLASYRY SPYPIPAVG AGVGAAAAA GGTPTPASA AAAPALAPP VGASPYQGY NLTNVDMSS FQGVDWGSM YGVGMYV
- >TC031986 RB
MLFENPPSA QIFPAYSVP PPILPLAPP PVSSAMGEG NGLIEHDLH GSLVPMNGS HSGSSGRST PNNSNDPAP GKLFVGGLS WQTSSEKLR EYFGMFGNV TDVLIMKDP VTQRSRGFG FITFSEPSS VDNVLKVPI HTLDGKKID PKHATPKNR PKQPNKTKK IFVGGVSQD TSADEVKAY FSQFGKVEE TVMLMDQQT KRHRGFGFV TFENEDVVD RVCEIHFHT IKNKKVECK KAQPKEAVQ ASAQLLGKR LMLSGLGMR MATPTAGAI AAATNPLAV VQAQAHVQA AAVAAAAQA QTAAVASYG KLFGAYPPL ASYRYSPYP IPAVGAGVG AAAAAGGTP TPASAAAAP ALAPPVGAS PYQGYNLTN VDMSSFQGV DWGSMYGVG MYV
- >TC031986 RA
atgggggaa ggcaacggc ctcatcgaa cacgacctt cacggctct ttggtaccg atgaacggt agccattct ggatcttcg ggtcgtagt actcccaac aactccaac gatccagca ccgggaaaa ctgtttgtc ggcggttta agttggcaa acttcaagc gagaagttg cgcgaatat ttcggcatg tttggaaac gtcaccgat gtattgata atgaaagat ccagtaacc cagaggagt cgcggtttc ggtttcatc acattttcg gagccctca agcgtcgac aacgtgctc aaagtgcca attcacact ttagacggt aaaaaaatc gacccgaag cacgccacg cccaaaaac cgcccgaaa cagccgaac aaaacgaaa aagatattc gtcggcggc gtgagccaa gacacctcc gccgacgag gtcaaagcc tacttcagt caattcggc aaagtggag gagacggtg atgctgatg gaccagcag acgaaacgc caccgtggc ttcggcttc gtcacgttc gagaacgag gacgtggtg gaccgcgtc tgcgagatc cacttccac accatcaag aacaagaag gtcgaatgc aagaaggct caacccaag gaggcggtg caagccagc gctcaactg ctcggcaag cgcctcatg ctcagcggc cttggcatg cgcatggcc actccgacg gcgggcgcc atcgccgcc gccaccaac cctttggct gtggttcaa gctcaggct cacgttcag gcggcggcg gtcgccgcc gccgcccag gcccagacc gccgccgtg gccagctac ggcaagttg ttcggggct tacccgccc ctcgccagc taccggtac tcgccttac cccatcccg gcggtgggc gccggcgtg ggggcggcg gcggcggcg ggcggcacc ccgacgccg gcttcggcc gccgccgct ccagcgcta gccccgccg gtgggcgcc agcccctac cagggctac aacctcacc aacgtagac atgtccagc tttcaagga gtggactgg ggctcgatg tacggcgtt ggcatgtac gtctga
- >TC031986 RB
atgcttttc gagaaccca cctagtgcc caaatattc cctgcgtac agtgtgcca cccccgatc ctgccgcta gcgccgcct cccgttagt tccgcaatg ggggaaggc aacggcctc atcgaacac gaccttcac ggctctttg gtaccgatg aacggtagc cattctgga tcttcgggt cgtagtact cccaacaac tccaacgat ccagcaccg ggaaaactg tttgtcggc ggtttaagt tggcaaact tcaagcgag aagttgcgc gaatatttc ggcatgttt ggaaacgtc accgatgta ttgataatg aaagatcca gtaacccag aggagtcgc ggtttcggt ttcatcaca ttttcggag ccctcaagc gtcgacaac gtgctcaaa gtgccaatt cacacttta gacggtaaa aaaatcgac ccgaagcac gccacgccc aaaaaccgc ccgaaacag ccgaacaaa acgaaaaag atattcgtc ggcggcgtg agccaagac acctccgcc gacgaggtc aaagcctac ttcagtcaa ttcggcaaa gtggaggag acggtgatg ctgatggac cagcagacg aaacgccac cgtggcttc ggcttcgtc acgttcgag aacgaggac gtggtggac cgcgtctgc gagatccac ttccacacc atcaagaac aagaaggtc gaatgcaag aaggctcaa cccaaggag gcggtgcaa gccagcgct caactgctc ggcaagcgc ctcatgctc agcggcctt ggcatgcgc atggccact ccgacggcg ggcgccatc gccgccgcc accaaccct ttggctgtg gttcaagct caggctcac gttcaggcg gcggcggtc gccgccgcc gcccaggcc cagaccgcc gccgtggcc agctacggc aagttgttc ggggcttac ccgcccctc gccagctac cggtactcg ccttacccc atcccggcg gtgggcgcc ggcgtgggg gcggcggcg gcggcgggc ggcaccccg acgccggct tcggccgcc gccgctcca gcgctagcc ccgccggtg ggcgccagc ccctaccag ggctacaac ctcaccaac gtagacatg tccagcttt caaggagtg gactggggc tcgatgtac ggcgttggc atgtacgtc tga
- >TC031986 RA
taaatttgt gtctttttt tttttctct aaaagtttg aagtgggat ttaacttac agtatacaa tctgtgttg aacagagtg tagaacggc caaaaagtg aacatgcag acatccatg atgcagata catacaatt gggattaac tgaaacaaa aaaaaatgt ctttattaa ttattttgt ttgtttata gttccgcaa tgggggaag gcaacggcc tcatcgaac acgaccttc acggctctt tggtaccga tgaacggta gccattctg gatcttcgg gtcgtagta ctcccaaca actccaacg atccagcac cgggaaaac tgtttgtcg gcggtttaa gttggcaaa cttcaagcg agaagttgc gcgaatatt tcggcatgt ttggaaacg tcaccgatg tattgataa tgaaagatc cagtaaccc agaggagtc gcggtttcg gtttcatca cattttcgg agccctcaa gcgtcgaca acgtgctca aagtgccaa ttcacactt tagacggta aaaaaatcg acccgaagc acgccacgc ccaaaaacc gcccgaaac agccgaaca aaacgaaaa agatattcg tcggcggcg tgagccaag acacctccg ccgacgagg tcaaagcct acttcagtc aattcggca aagtggagg agacggtga tgctgatgg accagcaga cgaaacgcc accgtggct tcggcttcg tcacgttcg agaacgagg acgtggtgg accgcgtct gcgagatcc acttccaca ccatcaaga acaagaagg tcgaatgca agaaggctc aacccaagg aggcggtgc aagccagcg ctcaactgc tcggcaagc gcctcatgc tcagcggcc ttggcatgc gcatggcca ctccgacgg cgggcgcca tcgccgccg ccaccaacc ctttggctg tggttcaag ctcaggctc acgttcagg cggcggcgg tcgccgccg ccgcccagg cccagaccg ccgccgtgg ccagctacg gcaagttgt tcggggctt acccgcccc tcgccagct accggtact cgccttacc ccatcccgg cggtgggcg ccggcgtgg gggcggcgg cggcggcgg gcggcaccc cgacgccgg cttcggccg ccgccgctc cagcgctag ccccgccgg tgggcgcca gcccctacc agggctaca acctcacca acgtagaca tgtccagct ttcaaggag tggactggg gctcgatgt acggcgttg gcatgtacg tctgaacgt aattttgct cactcttcg ttttagtta ggttttttg tgtttttat aaactgatg gacttccaa acgaaacat ttccaattg tcaatggcg gcgactcgt gcctatttt gactgtgcg ttttgatat ttcgattga ataaaatgt gtacataga tagaatctc atttgcatt aatgctatg ggcagttta gcatcacac cggcttgtt tattcctcc tgattgaac cgatgccgc cttctgctc atttagcat ttagcaata actcgcaat aatcagtat taaacgacg tgtagacga cgttctttt gaaaattac ccacgtggt tttgctcga tcctttacc tgtgtattc ctcttcatg gcaataaat ccccgctta ggttgtgtt tcgactgta cttaaatta ttcgtaggt acttaataa tttattacc gaattgact gaaacggcg atacttaat tatttatac ttaatgata caaaagtac actgtggtg caaaactgt agcaccacc cgattattg tcaaatttc aacacttta aatagttat cgaatcgaa tttttatag aatacttgc aaagaaaat cgatacgaa ccagccgct aatctgaat gcaactttt tttgctagt tgtgtaact gaacaatgg ggatgttac aaagtaaca agtaaactc attaaaatt tatgtgctt tt
- >TC031986 RB
gacttccgt aacacatcc aattgtttg ttcagctga ttgatgtcg acatgtggt ttgcaggat cgatcggga attcgacgc tcacatggc tgtcacggt ttaccccaa cgttaaact cgagtgcgt caggcgggc aagcatgtg gtgtggtgg gtcgtgtcg tcggcacag ccggcagaa gggcacagc tgcagtcgt attcgaatt tccagccgg cgagggtgc ttaacttgc tcacttgtt gtgtttttc gttagagtc gcgtaattt tggctgtga acatgcttt tcgagaacc cacctagtg cccaaatat tccctgcgt acagtgtgc cacccccga tcctgccgc tagcgccgc ctcccgtta gttccgcaa tgggggaag gcaacggcc tcatcgaac acgaccttc acggctctt tggtaccga tgaacggta gccattctg gatcttcgg gtcgtagta ctcccaaca actccaacg atccagcac cgggaaaac tgtttgtcg gcggtttaa gttggcaaa cttcaagcg agaagttgc gcgaatatt tcggcatgt ttggaaacg tcaccgatg tattgataa tgaaagatc cagtaaccc agaggagtc gcggtttcg gtttcatca cattttcgg agccctcaa gcgtcgaca acgtgctca aagtgccaa ttcacactt tagacggta aaaaaatcg acccgaagc acgccacgc ccaaaaacc gcccgaaac agccgaaca aaacgaaaa agatattcg tcggcggcg tgagccaag acacctccg ccgacgagg tcaaagcct acttcagtc aattcggca aagtggagg agacggtga tgctgatgg accagcaga cgaaacgcc accgtggct tcggcttcg tcacgttcg agaacgagg acgtggtgg accgcgtct gcgagatcc acttccaca ccatcaaga acaagaagg tcgaatgca agaaggctc aacccaagg aggcggtgc aagccagcg ctcaactgc tcggcaagc gcctcatgc tcagcggcc ttggcatgc gcatggcca ctccgacgg cgggcgcca tcgccgccg ccaccaacc ctttggctg tggttcaag ctcaggctc acgttcagg cggcggcgg tcgccgccg ccgcccagg cccagaccg ccgccgtgg ccagctacg gcaagttgt tcggggctt acccgcccc tcgccagct accggtact cgccttacc ccatcccgg cggtgggcg ccggcgtgg gggcggcgg cggcggcgg gcggcaccc cgacgccgg cttcggccg ccgccgctc cagcgctag ccccgccgg tgggcgcca gcccctacc agggctaca acctcacca acgtagaca tgtccagct ttcaaggag tggactggg gctcgatgt acggcgttg gcatgtacg tctgaacgt aattttgct cactcttcg ttttagtta ggttttttg tgtttttat aaactgatg gacttccaa acgaaacat ttccaattg tcaatggcg gcgactcgt gcctatttt gactgtgcg ttttgatat ttcgattga ataaaatgt gtacataga tagaatctc atttgcatt aatgctatg ggcagttta gcatcacac cggcttgtt tattcctcc tgattgaac cgatgccgc cttctgctc atttagcat ttagcaata actcgcaat aatcagtat taaacgacg tgtagacga cgttctttt gaaaattac ccacgtggt tttgctcga tcctttacc tgtgtattc ctcttcatg gcaataaat ccccgctta ggttgtgtt tcgactgta cttaaatta ttcgtaggt acttaataa tttattacc gaattgact gaaacggcg atacttaat tatttatac ttaatgata caaaagtac actgtggtg caaaactgt agcaccacc cgattattg tcaaatttc aacacttta aatagttat cgaatcgaa tttttatag aatacttgc aaagaaaat cgatacgaa ccagccgct aatctgaat gcaactttt tttgctagt tgtgtaact gaacaatgg ggatgttac aaagtaaca agtaaactc attaaaatt tatgtgctt tt
