Status
Please wait ...
Gene information
General information
TC004504 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3665859..3668237
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004504
    iBeetle screen iB_03772

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004504 RA
      MFNFFRRSK KETDRTKYK DADASPTLD MCKNNKVED 
      IVVPTTSEI GLRKIPTNA VISDEKNDS NETMCENTA 
      PVSYANIVK KPERESRGV SVKPCGHGT TAISPSVPI 
      LTGKRDTNT PPLSPKLEL KKTTKAEVP DSSDNLVNG 
      NDSEKKNLI NQEAKFQSQ LNDLSKQLS IRDAEATKL 
      RFQMEELQR DVFAKSAGM DRLETELQA AHKECELLR 
      QRIRYLETD LESYKKRNN ELLTELTEK SEFVTKYEN 
      DTKAKIEEL ESTIKELEV KITTLENEL DTLKKEKEK 
      LEEQRTIIL AERDAEKQK VDEILEQAT AQKQEIEKK 
      WKQDFEKLR TINIMKEQQ LLDDFEWKL REVQQTCKK 
      RLDDKDKSI EERLQEAYK EAERKMQEA EKMMQQVEA 
      MKSYEIEIE KLRGLTEDQ ENAIKEMME QQEQMKQAE 
      NNLKNETKR LRNLIELEK ENLQHIQLK HHQEILDKE 
      RALQQTLHQ KRTEIAMYW GGTAVTRVW PFEIRT
    CDS sequence
    • >TC004504 RA
      atgttcaat ttcttccgt cgctccaag aaagaaacc 
      gatcgcacc aagtacaaa gacgcagac gcatctcct 
      acattagat atgtgtaaa aataataaa gtcgaggat 
      atcgtcgtg cccacgacg tcggaaatc gggctacgt 
      aaaattcca acgaatgca gtgattagt gacgaaaaa 
      aacgactcg aacgaaaca atgtgcgaa aacactgct 
      cccgtttcg tacgccaac attgtgaaa aaaccggaa 
      agggagtcc agaggggtg agtgtgaag ccgtgcggg 
      cacgggacc acagcgata agcccaagt gtgcccatt 
      ctgaccggg aaaagggac accaacact ccgcccttg 
      agccctaag ctcgaactg aagaaaacc accaaagcg 
      gaagtacca gactccagt gataattta gtgaatgga 
      aacgatagt gagaagaaa aatctaata aaccaagaa 
      gcgaagttt caaagtcaa ctcaacgat ttatccaaa 
      caactgtcg atccgagat gcagaagca accaaactc 
      cgtttccag atggaagaa ctccagcga gatgttttt 
      gccaaatct gccggaatg gatcgactc gaaactgaa 
      ttacaagcc gcacacaag gaatgtgag cttttgcgc 
      caaagaatc cgttatctc gagactgat cttgaaagt 
      tacaaaaaa agaaacaac gaattattg accgaactg 
      actgaaaaa tcggaattc gtcaccaaa tacgaaaat 
      gacaccaaa gcaaaaatc gaggaactt gaaagcaca 
      atcaaggaa ctcgaagtg aaaatcacg actttagaa 
      aacgaattg gacacacta aaaaaggag aaggaaaaa 
      ctggaagaa cagcgcaca attatcctt gctgaacgc 
      gacgccgag aagcaaaaa gttgacgag atccttgaa 
      caagcaaca gcccagaaa caagaaatt gagaagaag 
      tggaagcaa gattttgag aaattacgc acaattaac 
      ataatgaag gagcaacaa ctcctcgat gactttgag 
      tggaaactt cgtgaagtc caacaaact tgtaaaaaa 
      cgcctcgat gataaagac aagagtatc gaagagcgg 
      ctccaagag gcgtacaag gaagcagaa cgcaagatg 
      caagaggcc gagaaaatg atgcagcaa gttgaggcg 
      atgaaatcg tacgaaatt gagattgag aaattgaga 
      gggttgact gaggaccag gaaaatgca atcaaggag 
      atgatggag caacaggag caaatgaaa caggcggag 
      aataatttg aaaaatgag acgaaacga ctcaggaat 
      ttgattgag ttggagaag gagaatctc caacacatc 
      caattaaaa caccaccaa gagatttta gacaaggaa 
      cgggctttg cagcaaact ttgcaccag aaacggact 
      gagattgcg atgtattgg ggaggaacg gctgttacg 
      agagtgtgg ccgtttgaa atccgaact tga
    mRNA sequence
    • >TC004504 RA
      attaactct atttccagc tcccccctc caaagcagt 
      accactgga ttttccaaa tattcttat tgctgaaat 
      ccgtcttgg atccatccc aacaaaacc cgccagtcg 
      ttgtccact tacagatcc atcatcctc tccagataa 
      aacccataa atcgcactt cgaaaaaaa tcgcgtcaa 
      gttgtctaa atttaaacc caagtgagc gtaacaaaa 
      cttcagttg tgttttacg aatcgtcaa cgaacgact 
      atgttctcg ctctaagtc cctcagaaa atgttcaat 
      ttcttccgt cgctccaag aaagaaacc gatcgcacc 
      aagtacaaa gacgcagac gcatctcct acattagat 
      atgtgtaaa aataataaa gtcgaggat atcgtcgtg 
      cccacgacg tcggaaatc gggctacgt aaaattcca 
      acgaatgca gtgattagt gacgaaaaa aacgactcg 
      aacgaaaca atgtgcgaa aacactgct cccgtttcg 
      tacgccaac attgtgaaa aaaccggaa agggagtcc 
      agaggggtg agtgtgaag ccgtgcggg cacgggacc 
      acagcgata agcccaagt gtgcccatt ctgaccggg 
      aaaagggac accaacact ccgcccttg agccctaag 
      ctcgaactg aagaaaacc accaaagcg gaagtacca 
      gactccagt gataattta gtgaatgga aacgatagt 
      gagaagaaa aatctaata aaccaagaa gcgaagttt 
      caaagtcaa ctcaacgat ttatccaaa caactgtcg 
      atccgagat gcagaagca accaaactc cgtttccag 
      atggaagaa ctccagcga gatgttttt gccaaatct 
      gccggaatg gatcgactc gaaactgaa ttacaagcc 
      gcacacaag gaatgtgag cttttgcgc caaagaatc 
      cgttatctc gagactgat cttgaaagt tacaaaaaa 
      agaaacaac gaattattg accgaactg actgaaaaa 
      tcggaattc gtcaccaaa tacgaaaat gacaccaaa 
      gcaaaaatc gaggaactt gaaagcaca atcaaggaa 
      ctcgaagtg aaaatcacg actttagaa aacgaattg 
      gacacacta aaaaaggag aaggaaaaa ctggaagaa 
      cagcgcaca attatcctt gctgaacgc gacgccgag 
      aagcaaaaa gttgacgag atccttgaa caagcaaca 
      gcccagaaa caagaaatt gagaagaag tggaagcaa 
      gattttgag aaattacgc acaattaac ataatgaag 
      gagcaacaa ctcctcgat gactttgag tggaaactt 
      cgtgaagtc caacaaact tgtaaaaaa cgcctcgat 
      gataaagac aagagtatc gaagagcgg ctccaagag 
      gcgtacaag gaagcagaa cgcaagatg caagaggcc 
      gagaaaatg atgcagcaa gttgaggcg atgaaatcg 
      tacgaaatt gagattgag aaattgaga gggttgact 
      gaggaccag gaaaatgca atcaaggag atgatggag 
      caacaggag caaatgaaa caggcggag aataatttg 
      aaaaatgag acgaaacga ctcaggaat ttgattgag 
      ttggagaag gagaatctc caacacatc caattaaaa 
      caccaccaa gagatttta gacaaggaa cgggctttg 
      cagcaaact ttgcaccag aaacggact gagattgcg 
      atgtattgg ggaggaacg gctgttacg agagtgtgg 
      ccgtttgaa atccgaact tgaacaaat ccataacga 
      ggaaaaaca ctacgctat ggagaccat cagaaggga 
      taaagacga agagtttca gaaagcaca agcagagtg 
      ggataaaaa attaaaaga gtgtctcaa ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY