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Gene information
General information
TC004502 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3681994..3683427
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004502
    iBeetle screen iB_03771

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004502 RA
      MLLPALFLL LQVSLGDFF ILRAPDCNI QISNLDPEP 
IVVDGTYTF LYAAPDASS VLVKSGETI IISCPGGEI 
TVGSTSFNS TVSATCVSN SDFSVGSAT INFNQIVCS 
WNPFHTARY TGKLCEKQG KEIEVGFVI NENFAREIT 
ICFDNANLN TLYSSYEIT KSIGHHESG VSRPFFIED 
DFYNLDVKV NSLYVRGGQ RTTINSLLG LPAGSTKYI 
QDGNDFYLA RGHFAAKAD FVYAPQQTA TFHYVNVAP 
QWQSFNGYN WNQVESDVR DYAEKNGID LKMYTGTYG 
VTTLPHEET GEETPLYLY IGSNGIQGI AVPELYWKV 
AYNPETQLG VALLGINNP YQKDINKSI ICEDVSAKI 
NWLHWNASD TKAGYSYAC EVDAFRKRV TYLPDFVVK 
GLLL
    CDS sequence
    • >TC004502 RA
      atgttgctt cctgcactc ttcctcctc ttgcaagtt 
tcattggga gattttttc attctccga gcgccagac 
tgcaacatc caaatctcg aatttggac cctgaaccc 
attgttgtg gacggcact tacacgttt ctttatgcc 
gctccggat gcctcatca gtacttgta aagtcggga 
gaaacaatc attatttcc tgtcccgga ggagaaata 
acagtcggt tcgactagc tttaattca accgtttca 
gcgacttgt gtttcaaac agcgatttt tcggttggt 
tccgccacc ataaatttc aaccaaatc gtctgctcc 
tggaacccg tttcacaca gcgaggtac acgggaaaa 
ttatgcgaa aaacaaggc aaagaaatc gaagttggg 
ttcgtcatt aatgaaaat ttcgcccgc gaaattaca 
atctgtttc gacaatgct aacttgaac actttgtac 
tcctcgtat gaaatcacc aagtctata ggccaccac 
gaaagtggc gtaagtcgc ccgtttttc atcgaagat 
gacttctac aacctcgac gtaaaagtc aacagtttg 
tatgtgcga ggggggcaa agaaccaca atcaacagt 
ctgttggga ctcccagct ggctcaacc aaatacatt 
caagacggc aacgacttc taccttgca agggggcac 
tttgccgcc aaagccgac ttcgtgtac gccccccaa 
cagaccgca acctttcac tatgtaaat gtcgccccc 
cagtggcag agtttcaac ggttataac tggaaccaa 
gtcgagagt gatgtccgg gattacgcc gaaaaaaac 
ggaattgat ttaaaaatg tacaccggg acttacggt 
gtcacaact ctgccgcat gaggaaacg ggggaggaa 
acgccgctt tatttgtac attgggagt aacgggatt 
caagggatt gcagtcccg gaactctac tggaaagtg 
gcctacaat cctgaaacg caactcgga gttgcgctg 
cttggaatt aataatccc tatcagaaa gatataaac 
aagagtatt atttgtgag gacgtttcg gcgaaaatt 
aattggcta cattggaac gcatctgat acgaaagct 
ggctattcg tacgcttgt gaagttgac gcgttcagg 
aaacgagtc acttattta cctgatttt gtagttaag 
ggactcctg ttgtaa
    mRNA sequence
    • >TC004502 RA
      taaaccttt aaaatgttg cttcctgca ctcttcctc 
ctcttgcaa gtttcattg ggagatttt ttcattctc 
cgagcgcca gactgcaac atccaaatc tcgaatttg 
gaccctgaa cccattgtt gtggacggc acttacacg 
tttctttat gccgctccg gatgcctca tcagtactt 
gtaaagtcg ggagaaaca atcattatt tcctgtccc 
ggaggagaa ataacagtc ggttcgact agctttaat 
tcaaccgtt tcagcgact tgtgtttca aacagcgat 
ttttcggtt ggttccgcc accataaat ttcaaccaa 
atcgtctgc tcctggaac ccgtttcac acagcgagg 
tacacggga aaattatgc gaaaaacaa ggcaaagaa 
atcgaagtt gggttcgtc attaatgaa aatttcgcc 
cgcgaaatt acaatctgt ttcgacaat gctaacttg 
aacactttg tactcctcg tatgaaatc accaagtct 
ataggccac cacgaaagt ggcgtaagt cgcccgttt 
ttcatcgaa gatgacttc tacaacctc gacgtaaaa 
gtcaacagt ttgtatgtg cgagggggg caaagaacc 
acaatcaac agtctgttg ggactccca gctggctca 
accaaatac attcaagac ggcaacgac ttctacctt 
gcaaggggg cactttgcc gccaaagcc gacttcgtg 
tacgccccc caacagacc gcaaccttt cactatgta 
aatgtcgcc ccccagtgg cagagtttc aacggttat 
aactggaac caagtcgag agtgatgtc cgggattac 
gccgaaaaa aacggaatt gatttaaaa atgtacacc 
gggacttac ggtgtcaca actctgccg catgaggaa 
acgggggag gaaacgccg ctttatttg tacattggg 
agtaacggg attcaaggg attgcagtc ccggaactc 
tactggaaa gtggcctac aatcctgaa acgcaactc 
ggagttgcg ctgcttgga attaataat ccctatcag 
aaagatata aacaagagt attatttgt gaggacgtt 
tcggcgaaa attaattgg ctacattgg aacgcatct 
gatacgaaa gctggctat tcgtacgct tgtgaagtt 
gacgcgttc aggaaacga gtcacttat ttacctgat 
tttgtagtt aagggactc ctgttgtaa ctgataaaa 
cacgattga aaaacaatg ataagaatt aagtattaa 
catttcttt gtttttccg atttcgtaa tctacaagt 
ggctcattt ccttgatca attgaggcc tcacaaggt 
agcagatta ctaaaacta tttgtgata aaaattcac 
caataaata attacccgt cca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY