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Gene information
General information
TC004502 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3681994..3683427
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004502
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03771

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004502 RA
      MLLPALFLL LQVSLGDFF ILRAPDCNI QISNLDPEP 
      IVVDGTYTF LYAAPDASS VLVKSGETI IISCPGGEI 
      TVGSTSFNS TVSATCVSN SDFSVGSAT INFNQIVCS 
      WNPFHTARY TGKLCEKQG KEIEVGFVI NENFAREIT 
      ICFDNANLN TLYSSYEIT KSIGHHESG VSRPFFIED 
      DFYNLDVKV NSLYVRGGQ RTTINSLLG LPAGSTKYI 
      QDGNDFYLA RGHFAAKAD FVYAPQQTA TFHYVNVAP 
      QWQSFNGYN WNQVESDVR DYAEKNGID LKMYTGTYG 
      VTTLPHEET GEETPLYLY IGSNGIQGI AVPELYWKV 
      AYNPETQLG VALLGINNP YQKDINKSI ICEDVSAKI 
      NWLHWNASD TKAGYSYAC EVDAFRKRV TYLPDFVVK 
      GLLL
    CDS sequence
    • >TC004502 RA
      atgttgctt cctgcactc ttcctcctc ttgcaagtt 
      tcattggga gattttttc attctccga gcgccagac 
      tgcaacatc caaatctcg aatttggac cctgaaccc 
      attgttgtg gacggcact tacacgttt ctttatgcc 
      gctccggat gcctcatca gtacttgta aagtcggga 
      gaaacaatc attatttcc tgtcccgga ggagaaata 
      acagtcggt tcgactagc tttaattca accgtttca 
      gcgacttgt gtttcaaac agcgatttt tcggttggt 
      tccgccacc ataaatttc aaccaaatc gtctgctcc 
      tggaacccg tttcacaca gcgaggtac acgggaaaa 
      ttatgcgaa aaacaaggc aaagaaatc gaagttggg 
      ttcgtcatt aatgaaaat ttcgcccgc gaaattaca 
      atctgtttc gacaatgct aacttgaac actttgtac 
      tcctcgtat gaaatcacc aagtctata ggccaccac 
      gaaagtggc gtaagtcgc ccgtttttc atcgaagat 
      gacttctac aacctcgac gtaaaagtc aacagtttg 
      tatgtgcga ggggggcaa agaaccaca atcaacagt 
      ctgttggga ctcccagct ggctcaacc aaatacatt 
      caagacggc aacgacttc taccttgca agggggcac 
      tttgccgcc aaagccgac ttcgtgtac gccccccaa 
      cagaccgca acctttcac tatgtaaat gtcgccccc 
      cagtggcag agtttcaac ggttataac tggaaccaa 
      gtcgagagt gatgtccgg gattacgcc gaaaaaaac 
      ggaattgat ttaaaaatg tacaccggg acttacggt 
      gtcacaact ctgccgcat gaggaaacg ggggaggaa 
      acgccgctt tatttgtac attgggagt aacgggatt 
      caagggatt gcagtcccg gaactctac tggaaagtg 
      gcctacaat cctgaaacg caactcgga gttgcgctg 
      cttggaatt aataatccc tatcagaaa gatataaac 
      aagagtatt atttgtgag gacgtttcg gcgaaaatt 
      aattggcta cattggaac gcatctgat acgaaagct 
      ggctattcg tacgcttgt gaagttgac gcgttcagg 
      aaacgagtc acttattta cctgatttt gtagttaag 
      ggactcctg ttgtaa
    mRNA sequence
    • >TC004502 RA
      taaaccttt aaaatgttg cttcctgca ctcttcctc 
      ctcttgcaa gtttcattg ggagatttt ttcattctc 
      cgagcgcca gactgcaac atccaaatc tcgaatttg 
      gaccctgaa cccattgtt gtggacggc acttacacg 
      tttctttat gccgctccg gatgcctca tcagtactt 
      gtaaagtcg ggagaaaca atcattatt tcctgtccc 
      ggaggagaa ataacagtc ggttcgact agctttaat 
      tcaaccgtt tcagcgact tgtgtttca aacagcgat 
      ttttcggtt ggttccgcc accataaat ttcaaccaa 
      atcgtctgc tcctggaac ccgtttcac acagcgagg 
      tacacggga aaattatgc gaaaaacaa ggcaaagaa 
      atcgaagtt gggttcgtc attaatgaa aatttcgcc 
      cgcgaaatt acaatctgt ttcgacaat gctaacttg 
      aacactttg tactcctcg tatgaaatc accaagtct 
      ataggccac cacgaaagt ggcgtaagt cgcccgttt 
      ttcatcgaa gatgacttc tacaacctc gacgtaaaa 
      gtcaacagt ttgtatgtg cgagggggg caaagaacc 
      acaatcaac agtctgttg ggactccca gctggctca 
      accaaatac attcaagac ggcaacgac ttctacctt 
      gcaaggggg cactttgcc gccaaagcc gacttcgtg 
      tacgccccc caacagacc gcaaccttt cactatgta 
      aatgtcgcc ccccagtgg cagagtttc aacggttat 
      aactggaac caagtcgag agtgatgtc cgggattac 
      gccgaaaaa aacggaatt gatttaaaa atgtacacc 
      gggacttac ggtgtcaca actctgccg catgaggaa 
      acgggggag gaaacgccg ctttatttg tacattggg 
      agtaacggg attcaaggg attgcagtc ccggaactc 
      tactggaaa gtggcctac aatcctgaa acgcaactc 
      ggagttgcg ctgcttgga attaataat ccctatcag 
      aaagatata aacaagagt attatttgt gaggacgtt 
      tcggcgaaa attaattgg ctacattgg aacgcatct 
      gatacgaaa gctggctat tcgtacgct tgtgaagtt 
      gacgcgttc aggaaacga gtcacttat ttacctgat 
      tttgtagtt aagggactc ctgttgtaa ctgataaaa 
      cacgattga aaaacaatg ataagaatt aagtattaa 
      catttcttt gtttttccg atttcgtaa tctacaagt 
      ggctcattt ccttgatca attgaggcc tcacaaggt 
      agcagatta ctaaaacta tttgtgata aaaattcac 
      caataaata attacccgt cca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY