Status
Please wait ...
Gene information
General information
TC004496 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 3752373..3758780
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004496
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03770

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: mostly legs
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_10501

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber not present - Number of animals showing the phenotype: 1

    vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 2
    vitellarium size decreased - Number of animals showing the phenotype: 2

    mature egg not fused - Number of animals showing the phenotype: 3
    lateral oviduct degenerated - Number of animals showing the phenotype: 3
    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004496 RA
      MTSIEEKIR KLGQNRDIE GLQDYVSNL SLEELVNVI 
      RTRLTNSNF TYTWNYILE GLAESPNSH NKRYGVVLS 
      VIEELDRSE VSSFHSNAI TSRICTELA KFQTEHLLK 
      LCDFCVESL QSGRVSQMS WKEILPQLL NVLSKKEHV 
      DVEGEEISG IEYKHQVIN NICTLQWSP NIVVNLTQM 
      FIDLSLSKE EHLQVVNKL AKQLEKMTP QEVPPFVYQ 
      LLKFCKNSS ASVIFIKLQ HYFALRYHD KFLDQTASS 
      SESSEMDLI EDASSQDIL EAEATVLYH IHSLAFVGH 
      NCIKDHLNF LKNMLRTPE FILHPFQLS VLLTLSTIP 
      RYEERVFEI IRSCVSRTF HEEQKKISS ASFRNLVPG 
      KTRLEIIFG HVVTASAQD RDLVLQGLI NFAFVLLGV 
      GSALGRDPI AEKQWQLGS IILIKIIKR KRHTASIIV 
      ETLCNKIVT EQSVTQYIE CLYLMTRKL ALLMLENKN 
      TIISLMGGL IEIPLTSAL LVLDAIIPL TKVSPTIRD 
      HFILLLHKA LHSRVTETR QVAVTGYLK LLANLKISD 
      LAILSQGSY SSGYSLYTQ ISITQTSQC TPSAFSNEA 
      LSLEVLNNL EACFMQPAE VRIQLYTGL YDAVCLNPD 
      LGIAVLETI WPHFCDFYN TDEETAVPL KFDKITLTR 
      DINVSLQEP LGKLIYTIG LIVTKVAKD NNETPTVAR 
      ILKVLESIC QRMIKCELV HFELDDGTD LGDVMPESQ 
      HKLFVLKEA MSVYEALIG YKFHSWELD SENNGKQIK 
      GLFEGYLRL SNCLKTVNK PKKSDKNKT ENDTTLKKE 
      QKSSNRLKL PETVLDFQV LSKIMSFLH ETMVSWTTC 
      VQANVVKSK REFHQHAMQ ATLNLVTSL KHKKNIETQ 
      TAKVLYDHL CDIAMVIYE RIIKRMDEF ASFDCTTAV 
      LAAECFLLI LNIVNNHHG SNLKAFLSR VGGRDTSED 
      LVSHLSEFI QTYQKLFET DEDEISEDP EVKKMSLIV 
      INTLSFFAH QIPPNSNTL ALQMMEWTK NYALNNDLK 
      GRQVVNGFI TLLFECHVN FKVSLTFFE QVSDHIGDV 
      TGVITEEEH TSEEMKVLN EQTVNTVFT CLCNNIKSV 
      LEDIDWVIA RLKAEFSLM TYPNDEGLE RRRENLQSR 
      ERGVCCQLC FIVTILTNL SNCTIPPGG LSEALLKNL 
      FLLYCTLSS LTKHFVLRS SKINPVFQN AKFERLVKL 
      AGKQLAPAV YKLITYIEE TQKEEPESP SKKSKKKVE 
      STTLKSKVL KETRLIPKV VYEIEQFGK LVAQLSNKT 
      KIDLAKFMG QGTVRGFRF LNWEQVVEK ANDGAPQTQ 
      DSEENNEES DEETSPPPS KQRKT
    CDS sequence
    • >TC004496 RA
      atgacttcc attgaggag aaaatacgt aaattgggc 
      caaaatcgc gacattgag ggcctgcaa gactacgtt 
      agcaattta agtttggag gaacttgta aatgtgatt 
      cggacacga ttaactaac tcgaatttt acgtatact 
      tggaattac attctggaa ggtttggcg gaaagtccc 
      aactctcat aacaagcgc tatggggtt gttttgagt 
      gtcattgaa gaattggac aggagtgag gtttcaagc 
      tttcacagt aatgctatt acgagccga atttgtacg 
      gaacttgct aagttccaa acggaacat ttgttaaaa 
      ttgtgtgat ttttgtgtt gagtcttta cagtcgggc 
      agagtttca caaatgagt tggaaagag atattgccc 
      caattattg aacgtttta agtaagaaa gaacatgtt 
      gatgtggag ggtgaagaa atatcaggc attgaatat 
      aagcatcaa gtcattaac aacatttgc actttgcag 
      tggtctcct aatatcgtt gttaattta actcagatg 
      tttatcgac ctttctctt tctaaagaa gaacacttg 
      caagtcgtt aacaagttg gcaaaacag ttggaaaaa 
      atgacgccg caagaggtc ccacctttt gtttaccag 
      ttgcttaaa ttttgtaaa aatagcagt gctagcgtc 
      attttcata aaattgcag cactatttc gccctgcga 
      tatcatgat aaattcttg gaccaaacc gcctcaagt 
      tcagagagt tcagaaatg gatttaatc gaggatgct 
      tcaagtcaa gacattcta gaagcagaa gcaacagtt 
      ctgtaccac atccacagt ttagctttc gtaggccac 
      aactgcatt aaagaccat ttaaatttc ctaaaaaac 
      atgttgagg acacccgaa tttattctc caccccttc 
      caattgagt gtcctactg accctatcc acaatacca 
      cgctacgag gaacgagtt tttgaaatc atcaggtcg 
      tgtgtctct cgaacattt cacgaagaa cagaagaaa 
      atttcctcg gcttcgttc aggaacttg gtgcctggc 
      aaaaccaga cttgaaata attttcgga catgttgtg 
      accgcaagt gcccaagac cgtgacttg gttcttcag 
      ggacttatc aattttgcc tttgtgcta cttggagtg 
      ggctctgct ttgggtcga gacccaatc gctgaaaaa 
      cagtggcaa ttagggagt attatctta attaagata 
      attaaacgg aaacggcac actgcgtcc atcatagtg 
      gaaactctg tgtaataaa atagtgacc gaacagtca 
      gtgactcaa tatattgaa tgtctttat ttgatgact 
      cgaaaattg gcgcttcta atgctagaa aacaaaaat 
      acgattatt tcgctaatg ggaggcttg attgagata 
      ccattaacc agtgcttta ctagtacta gacgcgatt 
      attccactc actaaagtc tcacctacc atccgagac 
      catttcatt ctcttgttg cacaaagca ctccattca 
      agagtgacc gaaacgcgc caagttgca gtcactggt 
      tacttaaaa ttattagca aatttaaaa atctcagat 
      ttagctatt ttgagtcaa ggttcttac tcatcaggt 
      tattcgtta tatactcaa atttccatt actcaaaca 
      tcacaatgc acaccaagt gccttcagt aacgaagca 
      ctctcttta gaagttttg aacaacctg gaggcgtgc 
      ttcatgcaa ccagccgaa gtcagaatt cagctatac 
      acaggtctc tacgacgct gtttgtttg aatcccgat 
      ttggggatc gcagttttg gaaacgatt tggccacat 
      ttttgcgat ttttacaat actgacgag gaaacggcc 
      gttccgttg aagtttgat aaaattaca ctcactcga 
      gacatcaat gtttcgtta caagagcct ttgggtaaa 
      ttaatttac acaatcggg cttattgtc acaaaagtg 
      gcgaaagat aacaacgaa actcccaca gttgcccga 
      attctaaag gtcctagaa tcaatttgt caaagaatg 
      attaagtgt gaattggtt cattttgaa ttggatgac 
      ggtaccgat ttgggcgat gtaatgccc gaatctcag 
      cacaaactt ttcgttctg aaggaagca atgagtgtt 
      tatgaggct ttgattggg tacaagttt cactcgtgg 
      gaactagac tcggaaaat aacggcaaa caaatcaag 
      ggtttgttt gaaggttac ttgcgactg tccaattgt 
      ttaaaaacc gttaacaaa ccaaaaaaa tcggacaag 
      aacaaaaca gaaaacgac acaacgttg aaaaaagaa 
      caaaagtca tcaaacaga ttaaaatta ccggagacg 
      gtgctcgat ttccaagtt ttgtcaaaa attatgagt 
      tttctacac gagacaatg gtttcgtgg accacatgc 
      gtccaagcc aatgttgtt aaatcaaag agagaattt 
      caccaacac gccatgcaa gcaactctc aatttagtt 
      acctcacta aaacacaaa aaaaatatt gaaacgcag 
      actgctaaa gtactttat gaccacttg tgcgatatt 
      gcgatggtc atttatgaa agaataatc aaacgaatg 
      gacgaattt gcaagtttt gattgcaca acagctgtt 
      ttagcagct gagtgtttt ctgttaatt ttgaacatt 
      gttaacaac caccatggg agtaatttg aaagcattt 
      ttgagtcga gttggtggt agagacaca agcgaagac 
      cttgtcagc catttgagc gagtttatc caaacttat 
      cagaaatta tttgaaacg gatgaagac gaaatttcc 
      gaggatcca gaagttaaa aaaatgtcg ctcattgtg 
      atcaatact ctgagcttc ttcgcacat caaattccc 
      ccaaatagc aatacctta gcgttacaa atgatggaa 
      tggacgaaa aattacgct cttaataac gatttaaaa 
      ggcagacaa gtcgtcaac gggtttatc acgttattg 
      tttgaatgc cacgttaac ttcaaagtt agtttaact 
      ttttttgaa caggtgtct gaccacatt ggtgatgtc 
      acaggtgtg atcactgag gaggaacac accagtgaa 
      gaaatgaaa gttttgaat gaacaaacc gtaaatacc 
      gtttttacg tgtttgtgt aacaatatc aaatcagtt 
      cttgaagat atcgattgg gttatcgca cgtttgaaa 
      gctgaattt tctctaatg acttatccc aatgatgaa 
      ggtttggag agaagacgt gaaaactta caatcacgc 
      gaacgtgga gtttgttgc cagttatgt ttcatagtc 
      acaatttta acaaatttg agcaattgt actatccct 
      cccgggggg ctgtctgag gccctttta aaaaatttg 
      tttttgttg tattgcacg ttgagttcg ctaacaaaa 
      catttcgtt ttgcggtcg tccaaaatc aacccggtc 
      ttccaaaat gccaaattt gagcgatta gttaaatta 
      gctggcaaa caattggct ccagctgtt tacaagctg 
      attacttat atcgaggag actcagaag gaagagcca 
      gaatcgccg tcgaaaaag tcaaagaaa aaagttgaa 
      tcgaccact ttgaaaagc aaagtgctg aaggagaca 
      cgacttata ccgaaagta gtttatgaa attgaacag 
      tttggtaaa ttagttgcg cagttgtct aacaagact 
      aaaattgat ttggccaag ttcatgggt caggggaca 
      gttcgaggt tttaggttt ttgaattgg gaacaagtt 
      gtggagaag gctaatgat ggggcacca caaacacaa 
      gatagtgaa gaaaataat gaagagagt gacgaggaa 
      acgtcacca cctccgtca aaacaacgc aaaacttaa
    mRNA sequence
    • >TC004496 RA
      agtcaactg aaataaaca attccacat tcaaattct 
      acaatttct gtgcatttt ccaataatt cggaatgaa 
      ttcgccaat ttcgcgaat tattagacg aagcccctt 
      gcttctgag acaaggaag tgtttcaaa aaatttcgt 
      acgcacgga gctatgcgg tggagtcct tggagagaa 
      tatgccgtt caagtttca caacaggtt cactttcac 
      cctaaaatg ttggctatt ttttgtgcc cgcattttg 
      ggcccctca aatcgtcgt ggcttacca acttctcaa 
      atagttatc aatacaaat tgtagtttt tctaataaa 
      tgattgctt tttaaattt agcgcgaaa tttcaatag 
      tttacaaac aactgccca cgacataac ctcatattt 
      tcaaaaagt ttcgctcca aaatgactt ccattgagg 
      agaaaatac gtaaattgg gccaaaatc gcgacattg 
      agggcctgc aagactacg ttagcaatt taagtttgg 
      aggaacttg taaatgtga ttcggacac gattaacta 
      actcgaatt ttacgtata cttggaatt acattctgg 
      aaggtttgg cggaaagtc ccaactctc ataacaagc 
      gctatgggg ttgttttga gtgtcattg aagaattgg 
      acaggagtg aggtttcaa gctttcaca gtaatgcta 
      ttacgagcc gaatttgta cggaacttg ctaagttcc 
      aaacggaac atttgttaa aattgtgtg atttttgtg 
      ttgagtctt tacagtcgg gcagagttt cacaaatga 
      gttggaaag agatattgc cccaattat tgaacgttt 
      taagtaaga aagaacatg ttgatgtgg agggtgaag 
      aaatatcag gcattgaat ataagcatc aagtcatta 
      acaacattt gcactttgc agtggtctc ctaatatcg 
      ttgttaatt taactcaga tgtttatcg acctttctc 
      tttctaaag aagaacact tgcaagtcg ttaacaagt 
      tggcaaaac agttggaaa aaatgacgc cgcaagagg 
      tcccacctt ttgtttacc agttgctta aattttgta 
      aaaatagca gtgctagcg tcattttca taaaattgc 
      agcactatt tcgccctgc gatatcatg ataaattct 
      tggaccaaa ccgcctcaa gttcagaga gttcagaaa 
      tggatttaa tcgaggatg cttcaagtc aagacattc 
      tagaagcag aagcaacag ttctgtacc acatccaca 
      gtttagctt tcgtaggcc acaactgca ttaaagacc 
      atttaaatt tcctaaaaa acatgttga ggacacccg 
      aatttattc tccacccct tccaattga gtgtcctac 
      tgaccctat ccacaatac cacgctacg aggaacgag 
      tttttgaaa tcatcaggt cgtgtgtct ctcgaacat 
      ttcacgaag aacagaaga aaatttcct cggcttcgt 
      tcaggaact tggtgcctg gcaaaacca gacttgaaa 
      taattttcg gacatgttg tgaccgcaa gtgcccaag 
      accgtgact tggttcttc agggactta tcaattttg 
      cctttgtgc tacttggag tgggctctg ctttgggtc 
      gagacccaa tcgctgaaa aacagtggc aattaggga 
      gtattatct taattaaga taattaaac ggaaacggc 
      acactgcgt ccatcatag tggaaactc tgtgtaata 
      aaatagtga ccgaacagt cagtgactc aatatattg 
      aatgtcttt atttgatga ctcgaaaat tggcgcttc 
      taatgctag aaaacaaaa atacgatta tttcgctaa 
      tgggaggct tgattgaga taccattaa ccagtgctt 
      tactagtac tagacgcga ttattccac tcactaaag 
      tctcaccta ccatccgag accatttca ttctcttgt 
      tgcacaaag cactccatt caagagtga ccgaaacgc 
      gccaagttg cagtcactg gttacttaa aattattag 
      caaatttaa aaatctcag atttagcta ttttgagtc 
      aaggttctt actcatcag gttattcgt tatatactc 
      aaatttcca ttactcaaa catcacaat gcacaccaa 
      gtgccttca gtaacgaag cactctctt tagaagttt 
      tgaacaacc tggaggcgt gcttcatgc aaccagccg 
      aagtcagaa ttcagctat acacaggtc tctacgacg 
      ctgtttgtt tgaatcccg atttgggga tcgcagttt 
      tggaaacga tttggccac atttttgcg atttttaca 
      atactgacg aggaaacgg ccgttccgt tgaagtttg 
      ataaaatta cactcactc gagacatca atgtttcgt 
      tacaagagc ctttgggta aattaattt acacaatcg 
      ggcttattg tcacaaaag tggcgaaag ataacaacg 
      aaactccca cagttgccc gaattctaa aggtcctag 
      aatcaattt gtcaaagaa tgattaagt gtgaattgg 
      ttcattttg aattggatg acggtaccg atttgggcg 
      atgtaatgc ccgaatctc agcacaaac ttttcgttc 
      tgaaggaag caatgagtg tttatgagg ctttgattg 
      ggtacaagt ttcactcgt gggaactag actcggaaa 
      ataacggca aacaaatca agggtttgt ttgaaggtt 
      acttgcgac tgtccaatt gtttaaaaa ccgttaaca 
      aaccaaaaa aatcggaca agaacaaaa cagaaaacg 
      acacaacgt tgaaaaaag aacaaaagt catcaaaca 
      gattaaaat taccggaga cggtgctcg atttccaag 
      ttttgtcaa aaattatga gttttctac acgagacaa 
      tggtttcgt ggaccacat gcgtccaag ccaatgttg 
      ttaaatcaa agagagaat ttcaccaac acgccatgc 
      aagcaactc tcaatttag ttacctcac taaaacaca 
      aaaaaaata ttgaaacgc agactgcta aagtacttt 
      atgaccact tgtgcgata ttgcgatgg tcatttatg 
      aaagaataa tcaaacgaa tggacgaat ttgcaagtt 
      ttgattgca caacagctg ttttagcag ctgagtgtt 
      ttctgttaa ttttgaaca ttgttaaca accaccatg 
      ggagtaatt tgaaagcat ttttgagtc gagttggtg 
      gtagagaca caagcgaag accttgtca gccatttga 
      gcgagttta tccaaactt atcagaaat tatttgaaa 
      cggatgaag acgaaattt ccgaggatc cagaagtta 
      aaaaaatgt cgctcattg tgatcaata ctctgagct 
      tcttcgcac atcaaattc ccccaaata gcaatacct 
      tagcgttac aaatgatgg aatggacga aaaattacg 
      ctcttaata acgatttaa aaggcagac aagtcgtca 
      acgggttta tcacgttat tgtttgaat gccacgtta 
      acttcaaag ttagtttaa ctttttttg aacaggtgt 
      ctgaccaca ttggtgatg tcacaggtg tgatcactg 
      aggaggaac acaccagtg aagaaatga aagttttga 
      atgaacaaa ccgtaaata ccgttttta cgtgtttgt 
      gtaacaata tcaaatcag ttcttgaag atatcgatt 
      gggttatcg cacgtttga aagctgaat tttctctaa 
      tgacttatc ccaatgatg aaggtttgg agagaagac 
      gtgaaaact tacaatcac gcgaacgtg gagtttgtt 
      gccagttat gtttcatag tcacaattt taacaaatt 
      tgagcaatt gtactatcc ctcccgggg ggctgtctg 
      aggcccttt taaaaaatt tgtttttgt tgtattgca 
      cgttgagtt cgctaacaa aacatttcg ttttgcggt 
      cgtccaaaa tcaacccgg tcttccaaa atgccaaat 
      ttgagcgat tagttaaat tagctggca aacaattgg 
      ctccagctg tttacaagc tgattactt atatcgagg 
      agactcaga aggaagagc cagaatcgc cgtcgaaaa 
      agtcaaaga aaaaagttg aatcgacca ctttgaaaa 
      gcaaagtgc tgaaggaga cacgactta taccgaaag 
      tagtttatg aaattgaac agtttggta aattagttg 
      cgcagttgt ctaacaaga ctaaaattg atttggcca 
      agttcatgg gtcagggga cagttcgag gttttaggt 
      ttttgaatt gggaacaag ttgtggaga aggctaatg 
      atggggcac cacaaacac aagatagtg aagaaaata 
      atgaagaga gtgacgagg aaacgtcac cacctccgt 
      caaaacaac gcaaaactt aattgttac gtttattaa 
      tacaaaagt tattacaaa taaaattga aacttaatt 
      acgatgaac aacccgtaa agttcttcc cttagaaac 
      aacacttca aaactttgt atacaacat aacaccatg 
      aggtatagt ctttctcct gatgattga ggtaaataa 
      acctttatg ccacaaagt ccaaggggc ggttgaagc 
      cccctttgc ggttcatgt actgtctgt atttgcgtt 
      tcaagataa catgaaccc cacccacgt cattacctt 
      caactttct tccctttgg tggtatcaa aactacaaa 
      aacccatca acttcatca tttcctgtt catcggcac 
      ttttaccct ccaagtctg cggcagtga caaccggtc 
      tttccggct gcagcggtt ttgattttt tctcgacgg 
      atctggacc tggacctcg tcaaggacc gggagtacc 
      tccgtcggg gttccctct cgtgccgtc tttccctat 
      ccctggacc tctccttcg accttttcc gtctgtggc 
      gttctctct ctcgctccc tatccctgt cccgttccc 
      tatcccgcc gccgagacc cgtagggcg atttcgagc 
      gactccgac gtcccatca ctcacaaaa actcaaaaa 
      aaaacaaat tttttccac aaaaatttt gacaactgt 
      cacgatcga ttgagaaaa acgtcgatt ttagttatg 
      attttttac acgaaagcg ctagtgttt caaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY