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Gene information
General information
TC032010
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004494,TC004495
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 1669494..1688978
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032010
    iBeetle screen iB_03769
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    remarks: bad quality of preparation,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032010 RA
      MKINTKLLP IKLHYFFFM AAVGPILPQ LPLFGKDLG 
      ISPVVMGTV TGILPFTFL LTKPIFGLA VDVWRNQRK 
      TIFVLVIIL MTLSFGALY FLKLPSTVT FYFQFSENT 
      HLDSCPNAT ISVVCNSSC LTKPTNFVA SVIAPRAFK 
      ICALPVSEL IWSKNSTCE FICTGESDL NNSELFTFW 
      SFIMLMSVG TIGFNIVNS ISDAICFDV VDDQNLYGK 
      QRLWGSIGF GATGLIAGY AVDLFSKNH TNYAAAYVT 
      MLICSMFDL LVCFKLKLP VIEAPKNIF KDLKVLLTD 
      RYVKTFIVF TVFVGMLDG VIIYFLFWY LEDLAKETH 
      TTNVKLIEG LILAAACLG AERVFFSIS GKILKRLGH 
      VHCFTLCFF NYSLRLGLI ALVSNPWWV LPIEFLLQG 
      PTFALTYTV IVAYANEIA PAGMSATMQ GIAAGMGDG 
      TGYALGSVL GGVLYRFWG GRVTFVAFM AFGAACGCW 
      CFFQMDQNV HYNFGGGAG NSLLPVSAF SAYSLLRRC 
      NYFLTPQAP EIHAKAAPV QRIPPEDIS CKDNEKCFL 
      NLPSPPLFG YPHHLPPNE QLNTRLNAT SPCSSLYSA 
      NFRRQRGEK KPIPEEQKD EKYFERRRR NNQAAKKSR 
      DARKMREDQ IALRATILE HENAILRAQ VLTLREETS 
      SLRQMLLQK KALEIAARD TQICGS
    CDS sequence
    • >TC032010 RA
      atgaaaata aacacgaag ctcctaccc attaaatta 
      cactatttc ttttttatg gccgctgta ggtccaatc 
      ctcccccaa ctgcccctt ttcgggaaa gatctgggc 
      atttccccg gtggtcatg ggcacagta accgggatc 
      ttacccttc acattttta ctaacaaaa ccaattttc 
      ggcttggca gtggacgtt tggagaaac caacgaaaa 
      acaattttc gtacttgtg ataatcttg atgactctc 
      tcttttggc gctttatat tttctcaaa ttaccatca 
      acagtcacg ttttacttc caattttcg gaaaacacc 
      cacctggat tcgtgccca aacgctacg atttcggta 
      gtttgcaat tcgagttgt ttgacaaaa ccgactaat 
      tttgtggcg agtgtaatt gcgcctcgg gcttttaaa 
      atttgtgct ttaccagtt agcgagttg atttggtct 
      aaaaactcg acttgtgaa tttatttgt accggggaa 
      tcggacttg aataattcc gaattgttc actttttgg 
      agctttatt atgctcatg tctgtgggg acaataggt 
      ttcaatatt gttaattcg atcagcgac gctatttgt 
      ttcgacgtt gttgacgat caaaatctt tacggcaag 
      caacgactt tggggatca atcgggttt ggagccact 
      ggattaata gcaggatat gctgttgat ttgttttcc 
      aaaaatcac acaaattac gccgcagct tacgtaaca 
      atgcttatt tgttcaatg tttgattta ctggtatgt 
      tttaaattg aaattaccg gtcattgag gcccccaaa 
      aacatattc aaagacttg aaggtgtta ctaactgat 
      cgatacgtg aaaactttt atcgtattc acagttttt 
      gtgggaatg ttggatggt gtaataatt tatttcctc 
      ttttggtac ttggaagat ttagcaaaa gaaacacac 
      accacaaat gtgaaacta atcgagggt ttaatcctc 
      gcagctgct tgtttaggc gctgaacgc gtattcttt 
      tcaatatca ggaaaaatt ctaaaacgt ttgggacac 
      gtccactgc ttcactttg tgctttttt aattacagt 
      ttacgactg gggttgata gctttggtc tccaatcct 
      tggtgggtc cttccaatt gaatttttg ttacaaggg 
      cccactttt gcgctaact tatactgtg atagtggct 
      tatgctaac gaaatagcc cctgcagga atgtccgct 
      acgatgcaa ggaatcgca gctggaatg ggagacggc 
      actggttat gcattgggg agtgtcctc ggtggtgtg 
      ctttaccgg ttttggggc ggaagagtg acttttgtg 
      gcctttatg gcctttgga gcggcatgt ggatgttgg 
      tgttttttc caaatggat caaaatgtt cactacaat 
      tttggcgga ggcgccggg aacagtctc ctgccagtg 
      tcggctttc tcggcctac agcttgctg aggaggtgc 
      aattatttt ctgacacca caagcacca gaaattcac 
      gccaaagca gcacctgtt caaagaatc cctcctgaa 
      gatatctcg tgcaaagat aacgaaaaa tgtttctta 
      aacctacca tcaccaccc ctttttggc taccctcac 
      catcttcca ccaaacgag caactaaat actcgtctt 
      aatgcaaca tctccgtgc tcgtcttta tattccgcc 
      aactttcgg cgacaaaga ggtgagaag aaaccaata 
      ccggaggag caaaaagac gagaaatat ttcgaacga 
      agacgcagg aataatcaa gcggcgaaa aaatcaaga 
      gatgcgaga aaaatgaga gaagaccaa attgcgcta 
      agagccact attttggag cacgaaaat gcgatttta 
      agggcacag gtgttgact ttaagagag gaaacgtcg 
      tctctgagg caaatgctg ctccagaag aaagcgcta 
      gaaatcgcc gcaagagac acgcaaatt tgcggctct 
      tga
    mRNA sequence
    • >TC032010 RA
      acattagtg caacatgaa aataaacac gaagctcct 
      acccattaa attacacta tttcttttt tatggccgc 
      tgtaggtcc aatcctccc ccaactgcc ccttttcgg 
      gaaagatct gggcatttc cccggtggt catgggcac 
      agtaaccgg gatcttacc cttcacatt tttactaac 
      aaaaccaat tttcggctt ggcagtgga cgtttggag 
      aaaccaacg aaaaacaat tttcgtact tgtgataat 
      cttgatgac tctctcttt tggcgcttt atattttct 
      caaattacc atcaacagt cacgtttta cttccaatt 
      ttcggaaaa cacccacct ggattcgtg cccaaacgc 
      tacgatttc ggtagtttg caattcgag ttgtttgac 
      aaaaccgac taattttgt ggcgagtgt aattgcgcc 
      tcgggcttt taaaatttg tgctttacc agttagcga 
      gttgatttg gtctaaaaa ctcgacttg tgaatttat 
      ttgtaccgg ggaatcgga cttgaataa ttccgaatt 
      gttcacttt ttggagctt tattatgct catgtctgt 
      ggggacaat aggtttcaa tattgttaa ttcgatcag 
      cgacgctat ttgtttcga cgttgttga cgatcaaaa 
      tctttacgg caagcaacg actttgggg atcaatcgg 
      gtttggagc cactggatt aatagcagg atatgctgt 
      tgatttgtt ttccaaaaa tcacacaaa ttacgccgc 
      agcttacgt aacaatgct tatttgttc aatgtttga 
      tttactggt atgttttaa attgaaatt accggtcat 
      tgaggcccc caaaaacat attcaaaga cttgaaggt 
      gttactaac tgatcgata cgtgaaaac ttttatcgt 
      attcacagt ttttgtggg aatgttgga tggtgtaat 
      aatttattt cctcttttg gtacttgga agatttagc 
      aaaagaaac acacaccac aaatgtgaa actaatcga 
      gggtttaat cctcgcagc tgcttgttt aggcgctga 
      acgcgtatt cttttcaat atcaggaaa aattctaaa 
      acgtttggg acacgtcca ctgcttcac tttgtgctt 
      ttttaatta cagtttacg actggggtt gatagcttt 
      ggtctccaa tccttggtg ggtccttcc aattgaatt 
      tttgttaca agggcccac ttttgcgct aacttatac 
      tgtgatagt ggcttatgc taacgaaat agcccctgc 
      aggaatgtc cgctacgat gcaaggaat cgcagctgg 
      aatgggaga cggcactgg ttatgcatt ggggagtgt 
      cctcggtgg tgtgcttta ccggttttg gggcggaag 
      agtgacttt tgtggcctt tatggcctt tggagcggc 
      atgtggatg ttggtgttt tttccaaat ggatcaaaa 
      tgttcacta caattttgg cggaggcgc cgggaacag 
      tctcctgcc agtgtcggc tttctcggc ctacagctt 
      gctgaggag gtgcaatta ttttctgac accacaagc 
      accagaaat tcacgccaa agcagcacc tgttcaaag 
      aatccctcc tgaagatat ctcgtgcaa agataacga 
      aaaatgttt cttaaacct accatcacc acccctttt 
      tggctaccc tcaccatct tccaccaaa cgagcaact 
      aaatactcg tcttaatgc aacatctcc gtgctcgtc 
      tttatattc cgccaactt tcggcgaca aagaggtga 
      gaagaaacc aataccgga ggagcaaaa agacgagaa 
      atatttcga acgaagacg caggaataa tcaagcggc 
      gaaaaaatc aagagatgc gagaaaaat gagagaaga 
      ccaaattgc gctaagagc cactatttt ggagcacga 
      aaatgcgat tttaagggc acaggtgtt gactttaag 
      agaggaaac gtcgtctct gaggcaaat gctgctcca 
      gaagaaagc gctagaaat cgccgcaag agacacgca 
      aatttgcgg ctcttgatt gggaaacaa gaaggaaac 
      tgcaatctg tgatttttt tatttagtt tattgtatt 
      tacataaaa acacagttt ttacaaaat gatactatt 
      cattattaa agcctaggc acaactatc attagtctt 
      taagcaggt acctgacaa ttgttattt tttctttca 
      ataaataag caaatttag caaatattg attcttcca 
      attttttta aaaacattt ttaataaag ttaattagg 
      ggat