| General information | |||
| TC004489 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 3809491..3814978 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004489 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03768 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
- >TC004489 RA
MSGEETSAD RNVEIWKIK KLIKSLELA RGNGTSMIS LIIPPKDQI SRVSKMLAD EFGTASNIK SRVNRLSVL GAITSVQHR LKLYTKVPP NGLVIYCGT IVTDEGKEK KVNIDFEPF KPINTSLYL CDNKFHTEA LTALLADDN KFGFIVMDG NGALFGTLQ GNTREVLHK FTVDLPKKH GRGGQSALR FARLRMEKR HNYVRKVAE VATQLFITN DKPNIAGLI LAGSADFKT ELSQSDMFD PRLQAKVIK LVDVSYGGE NGFNQAIEL AAESLQNVK FIQEKKLIG RYFDEISQD TGKYCFGVE DTLRGLELG AVETLICWE NLDIQRYVL KNHTTGTET VLHLTPEQE KDKSHFTDK DSGVELELV ECQPLLEWL ANNYKNFGA TLEIITDKS QEGSQFVRG FGGIGGLLR YKVDFQNLQ LDEELDNGD DFDLDDY
- >TC004489 RA
atgtcagga gaggagact tcagccgac cgtaatgta gaaatctgg aaaataaag aaactgatc aaaagtttg gaactggcg agaggcaat ggaaccagc atgatttcg ttaattata ccgcccaaa gatcaaatc tcgagagtt agcaaaatg ttagctgat gaattcggc acagcctcc aatatcaag tcgagagtt aacagattg tcagtcttg ggggctatc acctcagtc cagcatcgc ctcaaactc tacacaaaa gtgccgcca aacggctta gtcatctac tgtggtacg atcgtgacg gatgaaggt aaagaaaag aaagtcaac atcgatttc gaaccgttc aaacctatc aatacctct ctgtactta tgcgacaat aagttccac acggaagct ctaacggcc ttactggca gacgataat aaattcggc tttatcgta atggacggt aacggagca ttattcggt acattacaa ggcaacact cgagaggtc ctccataag ttcaccgtc gatctacca aaaaagcac ggacgtggt ggtcaatcc gcgctgcgt ttcgcccgt ttgcgaatg gaaaaacgg cacaattac gtgcgaaaa gtggccgaa gtggcaaca caactcttt atcaccaat gataagccc aatatcgct ggcttgata ttggcggga agtgctgat tttaagact gaacttagt caatccgat atgtttgat cctagattg caagcgaaa gttataaaa ttagtcgat gtgtcgtac gggggcgaa aacgggttc aatcaagcg attgaactg gcggctgaa tcgctccaa aacgtcaaa ttcatccaa gaaaagaaa ctgattggc cgatacttt gacgaaatc agtcaagac accggcaaa tactgtttc ggtgttgaa gatacgcta agagggctc gaattaggt gctgttgaa acgctgatt tgttgggag aatcttgat attcaacga tacgtcttg aagaatcac acgactggt acagaaacc gtattacac ctaacacca gaacaggaa aaggacaag tcacatttc actgacaaa gacagcggc gtggaactg gaattggtg gagtgtcag cctttgctt gaatggctt gcaaacaac tataagaat tttggtgct actttagaa attattacc gataagtcg caagaaggg tcgcagttc gttcgaggt tttggagga attggaggt ttgttacga tacaaagtg gatttccag aatttacag ttagatgaa gaactagac aacggcgat gattttgat ctagacgat tattaa
- >TC004489 RA
ccagtttga ttgaataaa tgggaaatt ccggtcgga aagttggga aatcgtagg gtccttttc gaatttcct tctgcgaga ggctgtcac ctgccagtt ttattttgt gatcccctt tgtttcggc atgtgagtg actggaata ttttttccc gcactaacc aagtctccg ggtcgcgca tttctggtt ataaccagt taaagtggt taagttacc tgcataacc agtgaaaag tgactcaat caagtagtt cgtcggcat tggagaccg cgccatttt gcatcgggt cacattttt cgtattttt agttcatac gaaatccag cagaaatgt caggagagg agacttcag ccgaccgta atgtagaaa tctggaaaa taaagaaac tgatcaaaa gtttggaac tggcgagag gcaatggaa ccagcatga tttcgttaa ttataccgc ccaaagatc aaatctcga gagttagca aaatgttag ctgatgaat tcggcacag cctccaata tcaagtcga gagttaaca gattgtcag tcttggggg ctatcacct cagtccagc atcgcctca aactctaca caaaagtgc cgccaaacg gcttagtca tctactgtg gtacgatcg tgacggatg aaggtaaag aaaagaaag tcaacatcg atttcgaac cgttcaaac ctatcaata cctctctgt acttatgcg acaataagt tccacacgg aagctctaa cggccttac tggcagacg ataataaat tcggcttta tcgtaatgg acggtaacg gagcattat tcggtacat tacaaggca acactcgag aggtcctcc ataagttca ccgtcgatc taccaaaaa agcacggac gtggtggtc aatccgcgc tgcgtttcg cccgtttgc gaatggaaa aacggcaca attacgtgc gaaaagtgg ccgaagtgg caacacaac tctttatca ccaatgata agcccaata tcgctggct tgatattgg cgggaagtg ctgatttta agactgaac ttagtcaat ccgatatgt ttgatccta gattgcaag cgaaagtta taaaattag tcgatgtgt cgtacgggg gcgaaaacg ggttcaatc aagcgattg aactggcgg ctgaatcgc tccaaaacg tcaaattca tccaagaaa agaaactga ttggccgat actttgacg aaatcagtc aagacaccg gcaaatact gtttcggtg ttgaagata cgctaagag ggctcgaat taggtgctg ttgaaacgc tgatttgtt gggagaatc ttgatattc aacgatacg tcttgaaga atcacacga ctggtacag aaaccgtat tacacctaa caccagaac aggaaaagg acaagtcac atttcactg acaaagaca gcggcgtgg aactggaat tggtggagt gtcagcctt tgcttgaat ggcttgcaa acaactata agaattttg gtgctactt tagaaatta ttaccgata agtcgcaag aagggtcgc agttcgttc gaggttttg gaggaattg gaggtttgt tacgataca aagtggatt tccagaatt tacagttag atgaagaac tagacaacg gcgatgatt ttgatctag acgattatt aaattcctt gtaaggaca attaaacca gcattttgt aggagtggt gccgtttaa tataacgtg aagtggccg tattttgct catattaaa tgtgttgtt acaaatgat tgatataca taatttttt agttaattg ttaatttat gtaaatttc tgatcatag gtgattttg tacaagcct tgttttatt ttccagttt tgcgcagta aaatggatt tgagaattg atacttttc ataattatt ttaaattca agggtcaaa gttccccgg ggatatatt cttgtatgt ttgcatgat ctgtgagtt tttaccaag atatttttt attttatca aactaaatg gaattgttt aaagtacta aatataagt ttttctttt taaataatt ggatttgtt ccttttgac agttcctca acctccacg ttgtagtat agtagattt acattcctt aaaaattca aaggcaact acttctatg aaagcttgc gtctgtttt accagtttg tatcaagcc gcataattt tacatacaa attgtctaa atacgaatt tttacgtaa tagccactc attgtactt tttaacgaa attaaggat cttgtgcag gggttagtg accgcagaa gacccttaa ttgggccaa cgacctgtc accacatgt tgtaatttt gtttgtaat aaaattacc accatacaa gaaactgca gtttatttg ctgtttcta ccgagtgat caagaatga ctgtcagct ggcaacaat tcagttgta ttaaaacaa aattcaaag ctgccaact cacagtcct tgattattt tgtat
