Status
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Gene information
General information
TC004474 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4446291..4450643
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004474
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03766

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004474 RA
      MWLELVLNL VFLATCLSA TRYDGPEGC SWLPDNNDT 
      SSSKFSVTC RVRTLDGTG TNISALPAE ITSKLRLLC 
      SEVLFFESA LPTQGLQRL HELEELQIT NCKLLNIEA 
      NSFEGLYNV KRLAINTFN SDWSSSKTL DINADSLKG 
      LKELQILDL ANNNIRAVV DGTFCSLAN LQTLNLTRN 
      RIKSPERFG FNLPECSNS ELQNLDLSH NELRALTEN 
      SGFSRLRRL QQLDLKNNN ISDISGEAL AGLVSLRIL 
      NLANNKIES LPEGLFAGS RELREIHLQ NNSLFSLAK 
      GLFHRLEQL LILDLSGNQ LTSNHVDAG TFAGLIRLI 
      VLNLSHNAL TRIDSKTFK DLYFLQILD LRNNSIGFI 
      EDNAFIPLY NLHTLNLAE NRLNTIGPL LFNGLFVLS 
      KLTLNNNLV VNIDSTAFR NCSALKELD LSSNALQEV 
      PDALKELSF LKTLDLGEN QISDFRNGS FKNLNQLTG 
      LRLIDNNIG NLTRGMLWD LPSLQVLNL AKNKIQSIE 
      RGTFDRNTQ LEAIRLDEN FLTDINGVF ATLASLLWL 
      NLSDNHLVW FDYAFIPSN LKWLDIHRN FIEHLGNYY 
      KIQDEIRVK TLDASHNRI TEISPMSIP NSVELLFIN 
      NNFIKVVKP NTFLDKTSL ARVDMYANE LVNLDMNAL 
      RLSPVPPNR SLPEIYLGG NPFHCDCTM EWLHPVNNM 
      SASRQYPRV MDLDNVMCK MTHSRGMAH MPLTRAKTS 
      DFLCTYETH CFALCHCCD FDACDCEMT CPNNCSCYH 
      DQTWNTNVV DCSGQNAIE IPHRIPMDA TEVYLDGND 
      FRELQNHVF IGRKNMRVL FVNNSGIET IQNSTFNGL 
      HTLQTLHLE DNKIYALEG YEFEHLSNL KELYLQNNV 
      ISSIGNKTL EPLKSLEIL RLDGNKLVV FPVWQLSSN 
      SHLTEISLA NNRWSCRCK FLQEFTAWV ADNAPKVVD 
      SASIQCYNG EATPQRRDL DFNSTACSD FYAGSSVID 
      SMLVSDYWK MVVVTLCIV ILLLLGVVL WFVFRDPVR 
      VWLYSRYGI RLCSFRAAA AKQYEDRDK LYDAFVCYS 
      PKDEEWVVQ ALAAELESN FQLCLHYRD LPHAAYLQH 
      AAPAVLEAA EASRRVLII LTRNFLQTE WSRFELRQA 
      LHEALKGRV FKLVILEEG PLPEAELDP ELRPYLKTG 
      NRVRWGEKR FWEKLRYAM PSGEPRGKI NANYRRNIN 
      NYTIDSRVV ANGTHSHTY PEKIKTQGP PSPGMQMFP 
      PPAYSAGAP HEPDDANYS SATTATPSP RPMRRAQEP 
      RPISDHIYS SIDSDYSTL ERGGSGRRG PPWRPHVMM 
      QTAGVNNGG QAYLV
    CDS sequence
    • >TC004474 RA
      atgtggctg gaactagtg ttgaatttg gtgtttctg 
      gcgacttgt ttgtccgca acgcgctac gatggtccc 
      gaggggtgc tcctggtta ccggataac aacgatact 
      tcttcgtca aagttcagc gtgacgtgc agagtgagg 
      actttggac ggcaccggc accaacatc agtgctttg 
      cccgccgaa atcacttcg aaattgcgt ttgttatgc 
      agtgaggtg ttgttcttc gaaagcgcg cttcccact 
      caagggctg cagcggctt cacgaactg gaagaatta 
      caaattacc aactgcaaa ctcctcaac atcgaggcg 
      aactcgttc gagggcctg tacaatgtg aagaggttg 
      gcaattaac acgttcaat tcggattgg agttcgagt 
      aagacactt gacatcaac gccgacagc ttgaaggga 
      ctcaaagaa ctgcaaatc ctggatctg gccaataat 
      aatatccgg gctgtcgtc gatggcact ttctgttcg 
      ctcgcaaat ctgcaaaca ctcaatctg acgaggaat 
      cgaattaaa agtccggaa cggtttggt tttaattta 
      ccggagtgc agtaacagt gaattgcaa aatctcgat 
      ttgtcacac aatgagctg agagcgctg acggagaac 
      tcgggtttt tcacgactg cgaagactg cagcagctc 
      gacttgaag aataacaac atcagcgac atttccggt 
      gaggccctc gccggatta gtgtctctc agaattttg 
      aatttggca aataacaaa atcgaaagc cttcctgaa 
      gggttgttt gccggctcg cgcgaattg cgtgagatt 
      catcttcag aataattcg ctcttttcg ttggccaaa 
      gggcttttc catcggtta gaacagttg ctgattctt 
      gatttgtcc ggaaatcaa ctgacaagt aatcacgtc 
      gatgcggga actttcgct ggtcttatt cgtttgatt 
      gttttgaat ttgtctcac aatgctttg actcgcatt 
      gacagcaaa actttcaaa gatttgtat tttcttcaa 
      atattggat ttgaggaat aactcaatc ggttttatc 
      gaggacaac gcttttatt cctctctac aatttgcat 
      actttgaat ctggctgaa aatcggctc aacaccatc 
      ggcccattg ttattcaac ggtttgttc gttctcagc 
      aaactcact ctaaacaat aatttggtg gttaacatc 
      gattcaaca gcattccgt aattgttcg gctctgaaa 
      gagctcgat ctgagttcg aatgctctt caggaagtg 
      cctgacgca ttgaaggag ctttccttt ttgaaaacg 
      ctggatttg ggcgagaac cagattagc gattttcgg 
      aacggttcg tttaaaaat ttgaatcaa ctgaccggc 
      ttgcgcctc atcgataac aacattggg aacttaacc 
      cgcgggatg ttgtgggat ttgccgagc ttacaagtg 
      cttaatctc gccaaaaac aagatccaa agtatcgaa 
      agaggaact tttgatcga aacacccaa ctcgaagcg 
      atccgtctc gacgagaat ttcctaaca gacattaac 
      ggtgtgttc gctactctc gcgagtttg ttgtggctg 
      aacttgtcc gataatcac ttggtgtgg ttcgattac 
      gctttcatt cccagcaac ctgaaatgg ctggacatt 
      catcgcaat ttcatcgaa cacctgggt aattattac 
      aaaatccag gacgagatc cgagtgaaa actctcgat 
      gctagtcat aaccgcatt acggaaatt tcgccgatg 
      tctatcccc aacagcgtc gagcttttg ttcatcaac 
      aataatttc atcaaagtc gtaaaaccg aacacgttc 
      ctggacaag actagtctg gcccgcgtg gacatgtac 
      gccaatgag ttagtaaat ctggacatg aacgcgctg 
      aggctgtcg ccggtgccg ccgaaccgg tccctgccc 
      gaaatctac ctgggtggc aaccccttt cactgcgac 
      tgcaccatg gaatggctg cacccggtc aacaacatg 
      tccgcctcc aggcagtac ccccgggtg atggacctc 
      gacaatgtc atgtgcaaa atgacccac tcgcggggc 
      atggcccac atgcccctg acccgggcc aaaaccagt 
      gactttttg tgcacctat gagacgcac tgttttgcc 
      ctttgccac tgttgtgac ttcgacgct tgtgattgc 
      gaaatgacg tgccctaac aattgttct tgctaccac 
      gatcagacc tggaacacc aacgttgtc gattgttcc 
      ggtcagaac gccatcgag atccctcac aggattccg 
      atggacgcc accgaggtg tatctcgac ggcaacgac 
      ttcagggaa ctgcaaaac cacgttttt atcgggcgg 
      aaaaatatg cgagtgttg ttcgttaat aacagtgga 
      atcgagacg attcagaat agtactttt aacggtctc 
      cacacgtta cagacgtta catctcgag gacaataaa 
      atttacgcg ttggagggt tacgaattc gagcatttg 
      tcgaatttg aaagaactc tatctacag aataatgtg 
      atttcgagc atcgggaat aaaactctg gagccgtta 
      aagagtttg gagattttg cgattggac ggtaataaa 
      ttagtggtg tttccggtg tggcaattg tcgtcgaac 
      tcccacttg accgagata agtttagcc aataatcgg 
      tggtcgtgc cggtgcaaa ttcctccaa gagttcacc 
      gcttgggtt gctgataac gccccgaaa gtcgtcgat 
      agtgcctcg atccagtgc tacaacggc gaagcaacg 
      cctcagcgt cgcgacctt gacttcaac agcaccgcg 
      tgcagtgac ttttacgcc ggaagttcc gttatagac 
      tcgatgtta gtttcggac tattggaaa atggtcgta 
      gtgacttta tgcattgtg attttactc ctcttgggc 
      gtcgttctc tggttcgtg tttcgtgat cccgttcgc 
      gtttggctt tactcgcgt tacggcata agactgtgc 
      agtttccgg gccgccgcc gccaaacaa tacgaagac 
      agggacaaa ttgtacgac gccttcgtg tgttacagt 
      cccaaagac gaagagtgg gttgtccag gcgttggcc 
      gccgaattg gagtcaaac ttccagctc tgtctccac 
      taccgagac ctaccacat gccgcctac ctgcagcac 
      gccgccccc gcagtcttg gaggcggcg gaagccagc 
      cgaagagtc ttaatcatc ctcactcgc aatttcctt 
      caaacggag tggtcccgt ttcgagctg cgtcaagcc 
      ctgcacgaa gcgctcaaa gggcgggtg tttaagttg 
      gttattctg gaggaaggg ccgctcccc gaagctgaa 
      ctcgatccc gagctcagg ccctactta aagactggc 
      aatcgagta cgatggggc gagaagcgg ttttgggag 
      aaactgaga tacgcgatg ccctcaggt gaaccgaga 
      ggaaagatt aacgccaat taccgaaga aacatcaat 
      aattacaca atcgattcg agggttgtt gcgaacgga 
      acccacagc catacgtat ccagagaag attaagact 
      cagggcccc ccttctcca ggcatgcaa atgttcccg 
      ccgccggcg tattcagct ggggcgccc cacgagccg 
      gacgatgcg aattactcc tcggcgacg actgccacg 
      ccttccccc aggccaatg agacgggcg caggaaccc 
      aggcccatc tccgaccac atttactcc agtatcgac 
      tccgattac agcaccctg gaacgtggg gggtccggc 
      aggaggggg cctccatgg agaccccac gttatgatg 
      cagactgca ggcgtcaac aacggcggc caggcctat 
      ctcgtgtga
    mRNA sequence
    • >TC004474 RA
      ttagtacaa cttcgactc ttgtggagg ttgattcgc 
      atgagcagc gcgcgtacg aactcattt cagtgattc 
      gtgatcgtc cggagtaaa cagtggctg tgatatgat 
      cgtttatta acagcaaat tagttgtaa acccgttga 
      gaataaacc tatcagtaa aatggtcag gctaaaata 
      ataaaaaaa gaaaaaata gtttcgcga tgtggctgg 
      aactagtgt tgaatttgg tgtttctgg cgacttgtt 
      tgtccgcaa cgcgctacg atggtcccg aggggtgct 
      cctggttac cggataaca acgatactt cttcgtcaa 
      agttcagcg tgacgtgca gagtgagga ctttggacg 
      gcaccggca ccaacatca gtgctttgc ccgccgaaa 
      tcacttcga aattgcgtt tgttatgca gtgaggtgt 
      tgttcttcg aaagcgcgc ttcccactc aagggctgc 
      agcggcttc acgaactgg aagaattac aaattacca 
      actgcaaac tcctcaaca tcgaggcga actcgttcg 
      agggcctgt acaatgtga agaggttgg caattaaca 
      cgttcaatt cggattgga gttcgagta agacacttg 
      acatcaacg ccgacagct tgaagggac tcaaagaac 
      tgcaaatcc tggatctgg ccaataata atatccggg 
      ctgtcgtcg atggcactt tctgttcgc tcgcaaatc 
      tgcaaacac tcaatctga cgaggaatc gaattaaaa 
      gtccggaac ggtttggtt ttaatttac cggagtgca 
      gtaacagtg aattgcaaa atctcgatt tgtcacaca 
      atgagctga gagcgctga cggagaact cgggttttt 
      cacgactgc gaagactgc agcagctcg acttgaaga 
      ataacaaca tcagcgaca tttccggtg aggccctcg 
      ccggattag tgtctctca gaattttga atttggcaa 
      ataacaaaa tcgaaagcc ttcctgaag ggttgtttg 
      ccggctcgc gcgaattgc gtgagattc atcttcaga 
      ataattcgc tcttttcgt tggccaaag ggcttttcc 
      atcggttag aacagttgc tgattcttg atttgtccg 
      gaaatcaac tgacaagta atcacgtcg atgcgggaa 
      ctttcgctg gtcttattc gtttgattg ttttgaatt 
      tgtctcaca atgctttga ctcgcattg acagcaaaa 
      ctttcaaag atttgtatt ttcttcaaa tattggatt 
      tgaggaata actcaatcg gttttatcg aggacaacg 
      cttttattc ctctctaca atttgcata ctttgaatc 
      tggctgaaa atcggctca acaccatcg gcccattgt 
      tattcaacg gtttgttcg ttctcagca aactcactc 
      taaacaata atttggtgg ttaacatcg attcaacag 
      cattccgta attgttcgg ctctgaaag agctcgatc 
      tgagttcga atgctcttc aggaagtgc ctgacgcat 
      tgaaggagc tttcctttt tgaaaacgc tggatttgg 
      gcgagaacc agattagcg attttcgga acggttcgt 
      ttaaaaatt tgaatcaac tgaccggct tgcgcctca 
      tcgataaca acattggga acttaaccc gcgggatgt 
      tgtgggatt tgccgagct tacaagtgc ttaatctcg 
      ccaaaaaca agatccaaa gtatcgaaa gaggaactt 
      ttgatcgaa acacccaac tcgaagcga tccgtctcg 
      acgagaatt tcctaacag acattaacg gtgtgttcg 
      ctactctcg cgagtttgt tgtggctga acttgtccg 
      ataatcact tggtgtggt tcgattacg ctttcattc 
      ccagcaacc tgaaatggc tggacattc atcgcaatt 
      tcatcgaac acctgggta attattaca aaatccagg 
      acgagatcc gagtgaaaa ctctcgatg ctagtcata 
      accgcatta cggaaattt cgccgatgt ctatcccca 
      acagcgtcg agcttttgt tcatcaaca ataatttca 
      tcaaagtcg taaaaccga acacgttcc tggacaaga 
      ctagtctgg cccgcgtgg acatgtacg ccaatgagt 
      tagtaaatc tggacatga acgcgctga ggctgtcgc 
      cggtgccgc cgaaccggt ccctgcccg aaatctacc 
      tgggtggca acccctttc actgcgact gcaccatgg 
      aatggctgc acccggtca acaacatgt ccgcctcca 
      ggcagtacc cccgggtga tggacctcg acaatgtca 
      tgtgcaaaa tgacccact cgcggggca tggcccaca 
      tgcccctga cccgggcca aaaccagtg actttttgt 
      gcacctatg agacgcact gttttgccc tttgccact 
      gttgtgact tcgacgctt gtgattgcg aaatgacgt 
      gccctaaca attgttctt gctaccacg atcagacct 
      ggaacacca acgttgtcg attgttccg gtcagaacg 
      ccatcgaga tccctcaca ggattccga tggacgcca 
      ccgaggtgt atctcgacg gcaacgact tcagggaac 
      tgcaaaacc acgttttta tcgggcgga aaaatatgc 
      gagtgttgt tcgttaata acagtggaa tcgagacga 
      ttcagaata gtactttta acggtctcc acacgttac 
      agacgttac atctcgagg acaataaaa tttacgcgt 
      tggagggtt acgaattcg agcatttgt cgaatttga 
      aagaactct atctacaga ataatgtga tttcgagca 
      tcgggaata aaactctgg agccgttaa agagtttgg 
      agattttgc gattggacg gtaataaat tagtggtgt 
      ttccggtgt ggcaattgt cgtcgaact cccacttga 
      ccgagataa gtttagcca ataatcggt ggtcgtgcc 
      ggtgcaaat tcctccaag agttcaccg cttgggttg 
      ctgataacg ccccgaaag tcgtcgata gtgcctcga 
      tccagtgct acaacggcg aagcaacgc ctcagcgtc 
      gcgaccttg acttcaaca gcaccgcgt gcagtgact 
      tttacgccg gaagttccg ttatagact cgatgttag 
      tttcggact attggaaaa tggtcgtag tgactttat 
      gcattgtga ttttactcc tcttgggcg tcgttctct 
      ggttcgtgt ttcgtgatc ccgttcgcg tttggcttt 
      actcgcgtt acggcataa gactgtgca gtttccggg 
      ccgccgccg ccaaacaat acgaagaca gggacaaat 
      tgtacgacg ccttcgtgt gttacagtc ccaaagacg 
      aagagtggg ttgtccagg cgttggccg ccgaattgg 
      agtcaaact tccagctct gtctccact accgagacc 
      taccacatg ccgcctacc tgcagcacg ccgcccccg 
      cagtcttgg aggcggcgg aagccagcc gaagagtct 
      taatcatcc tcactcgca atttccttc aaacggagt 
      ggtcccgtt tcgagctgc gtcaagccc tgcacgaag 
      cgctcaaag ggcgggtgt ttaagttgg ttattctgg 
      aggaagggc cgctccccg aagctgaac tcgatcccg 
      agctcaggc cctacttaa agactggca atcgagtac 
      gatggggcg agaagcggt tttgggaga aactgagat 
      acgcgatgc cctcaggtg aaccgagag gaaagatta 
      acgccaatt accgaagaa acatcaata attacacaa 
      tcgattcga gggttgttg cgaacggaa cccacagcc 
      atacgtatc cagagaaga ttaagactc agggccccc 
      cttctccag gcatgcaaa tgttcccgc cgccggcgt 
      attcagctg gggcgcccc acgagccgg acgatgcga 
      attactcct cggcgacga ctgccacgc cttccccca 
      ggccaatga gacgggcgc aggaaccca ggcccatct 
      ccgaccaca tttactcca gtatcgact ccgattaca 
      gcaccctgg aacgtgggg ggtccggca ggagggggc 
      ctccatgga gaccccacg ttatgatgc agactgcag 
      gcgtcaaca acggcggcc aggcctatc tcgtgtgat 
      aacttaact gactgacta ggtatacat agactcgta 
      ctgaatgaa caatattta aaatcataa cttgagtcg 
      aaaacgtgt aattttatt gtttgtatt atttttgta 
      cattacctg taaatagat caaatattt atttgtttt 
      tttttgtat cattttgtg tatattttt tacgacttg 
      caaaacgaa ttataataa atacgatgt gaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY