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Gene information
General information
TC004461 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4742889..4753366
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004461
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03764

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004461 RA
      MIESDSPIA SPLAGSGPG GHKPSFKST KLARRARSF 
      KDDFLGKIS QMRSPTGPG LRSHSPKER PSKSDMVEH 
      AYSTTKGPL QELENLWKQ TQATLKHFR DVVSKQKLE 
      MLPGNGTIV LDTVWQINL VVRSSINSD SSNAIFSAT 
      SHMYQSVAR LIKLCDDAL IDDKSSELT TENVNEIVA 
      QVEVAVQEL IRLVQEKIA HQQISYKTT PRSSYVNTS 
      LEMPAQRNS LPDIPLTPR ERQLLEQNS CGPPIVRSS 
      HSTESILRD SSPPPKPPL PDGHSEPSS CITPPPLPP 
      KKKNYKLHR LTDECSSLD NSPIASSLE RVSVRSKSP 
      EDSISLLSA SAGSLDSVL NHSRGEEDD EIKGIMERT 
      CDSEVDNHS ADIFGANGS HSSWEESSL SSNISGQIT 
      DYNRLSNTD SGFISSQSY RSSSYSKRS SQQSSVSSQ 
      WAQHTSKMS TETVKTEST MHSVHKEGD SLLTIKNFD 
      QKTSSSSSS SSLVSTIVN AGQLSITDA DETQPPAVP 
      QKTKRKERQ PSPYDNVPD SNLGGELLV TCPMHQSHQ 
      SLASSTSMS STMGQDSNK PPPLPPKKK HIMAYMEMF 
      GNCSHANEQ EFMRHSVHM VQSHWAQPP TPGLPTTQS 
      CSFSHTSHS SSRSSHTQI QILNVPPHS TMDQRVSSP 
      LHSPNPIIM ESNSPPPAL PPKQRSRNS SVKPSPPPT 
      PTVNEVKPQ SPVKTLPDL LEHTANSVT KTAEDKNEI 
      SEVNRCDID LMEEVNVDD WLVLKKPEE EGPDIRGGP 
      IDALIIQAT KATKNGVFM YQEAFLTTY RTFITPLDL 
      ITKLIRRYD HFSFKPNNK KLRSEAFAL IVRVVNDLT 
      VSDLDDQLQ CKLMHFVQQ LISSGEITL AKALRVKHL 
      ERYEAKQMS LKYTNVVTS LSLTARTST LLDFKSEQI 
      AEQMTLLDA DLFMKIEIP EVLIWAQEQ NEERSPNLT 
      RFTEHFNKM SYWARTRIL TAEGKDVRE KYFLKFIKI 
      MKHLRKINN FNSYLALLS ALDSAPIRR LEWQKHVQE 
      GLKEYCALI DSSSSFRAY RVALAETQP PCIPYIGLV 
      LQDLTFVHI GNSNLLPDG TINFSKRWQ QYNIVENMK 
      KFKKGIYTF KKQERIIQF FQNFDDFIG EDAMWQLSE 
      SIKPRGGKK ATS
    • >TC004461 RB
      MIESDSPIA SPLAGSGPG GHKPSFKST KLARRARSF 
      KDDFLGKIS QMRSPTGPG LRSHSPKER PSKSDMVEH 
      AYSTTKGPL QELENLWKQ TQATLKHFR DVVSKQKLE 
      MLPGNGTIV LDTVWQINL VVRSSINSD SSNAIFSAT 
      SHMYQSVAR LIKLCDDAL IDDKSSELT TENVNEIVA 
      QVEVAVQEL IRLVQEKIA HQQISYKTT PRSSYVNTS 
      LEMPAQRNS LPDIPLTPR ERQLLEQNS CGPPIVRSS 
      HSTESILRD SSPPPKPPL PDGVLFNIS HSEPSSCIT 
      PPPLPPKKK NYKLHRLTD ECSSLDNSP IASSLERVS 
      VRSKSPEDS ISLLSASAG SLDSVLNHS RGEEDDEIK 
      GIMERTCDS EVDNHSADI FGANGSHSS WEESSLSSN 
      ISGQITDYN RLSNTDSGF ISSQSYRSS SYSKRSSQQ 
      SSVSSQWAQ HTSKMSTET VKTESTMHS VHKEGDSLL 
      TIKNFDQKT SSSSSSSSL VSTIVNAGQ LSITDADET 
      QPPAVPQKT KRKERQPSP YDNVPDSNL GGELLVTCP 
      MHQSHQSLA SSTSMSSTM GQDSNKPPP LPPKKKHIM 
      AYMEMFGNC SHANEQEFM RHSVHMVQS HWAQPPTPG 
      LPTTQSCSF SHTSHSSSR SSHTQIQIL NVPPHSTMD 
      QRVSSPLHS PNPIIMESN SPPPALPPK QRSRNSSVK 
      PSPPPTPTV NEVKPQSPV KTLPDLLEH TANSVTKTA 
      EDKNEISEV NRCDIDLME EVNVDDWLV LKKPEEEGP 
      DIRGGPIDA LIIQATKAT KNGVFMYQE AFLTTYRTF 
      ITPLDLITK LIRRYDHFS FKPNNKKLR SEAFALIVR 
      VVNDLTVSD LDDQLQCKL MHFVQQLIS SGEITLAKA 
      LRVKHLERY EAKQMSLKY TNVVTSLSL TARTSTLLD 
      FKSEQIAEQ MTLLDADLF MKIEIPEVL IWAQEQNEE 
      RSPNLTRFT EHFNKMSYW ARTRILTAE GKDVREKYF 
      LKFIKIMKH LRKINNFNS YLALLSALD SAPIRRLEW 
      QKHVQEGLK EYCALIDSS SSFRAYRVA LAETQPPCI 
      PYIGLVLQD LTFVHIGNS NLLPDGTIN FSKRWQQYN 
      IVENMKKFK KGIYTFKKQ ERIIQFFQN FDDFIGEDA 
      MWQLSESIK PRGGKKATS
    CDS sequence
    • >TC004461 RA
      atgatagaa agcgacagt cctattgct tcacctctg 
      gccgggtcc ggtcctggc ggccacaaa ccctccttc 
      aaaagcacc aaactcgct cgccgcgcc cgttcgttt 
      aaagacgac tttttgggc aaaatctcg caaatgcgc 
      agccccaca ggcccgggc ctccgctcg cacagcccc 
      aaggagcgg ccgtccaag tcggacatg gtcgagcac 
      gcgtattcg acgaccaaa gggcctcta caagagctg 
      gaaaacctc tggaagcag acccaggcc acgctaaaa 
      cacttccga gacgtcgtc tcgaagcaa aagctcgaa 
      atgctgccc ggaaatggc accatagtg ctcgacaca 
      gtgtggcaa ataaacctc gttgtgcga tcgtcaatc 
      aactcggat tcgagcaat gcgattttt tcggccacc 
      agccacatg taccagagt gtggccagg ctgatcaaa 
      ctgtgcgat gatgcgctg attgatgac aaaagcagc 
      gaattgacg acggagaat gtcaacgaa atcgtggcg 
      caagtggag gtggcggtg caggagctg atcaggttg 
      gtccaggag aagatcgcg caccaacaa atttcttat 
      aagacaaca ccaaggagt tcgtacgtt aacaccagc 
      ctagaaatg ccagcacaa cgaaattct ttacctgat 
      attcctctg acaccaagg gagaggcag ctgttggag 
      cagaattcg tgtgggccg ccgattgtg agaagcagt 
      cacagcacg gagtcgatc ttgagggac tccagtccg 
      cctcccaag ccaccgcta ccagatggc cactcggaa 
      ccgagcagt tgtataaca ccacctcct ttacctcca 
      aagaagaaa aactacaaa ctgcatcga ttgacagac 
      gaatgttcg agtttggat aactcccct attgcttct 
      agtctagaa agagtctca gttcggtcg aaatcaccc 
      gaagactca atctccctg ttatcagca agtgcgggc 
      agtttagac tcggtccta aaccactct cgtggcgag 
      gaagacgac gaaattaaa ggcataatg gagcggaca 
      tgtgacagt gaagtcgat aatcattca gctgatatt 
      ttcggcgca aacgggtct cacagttcc tgggaggag 
      tcgtcatta agctcaaac atatccggt caaatcacc 
      gactacaat cgactatca aacaccgac tcaggcttt 
      atatcgtcg cagtcttac cgatcgtcg agttactcc 
      aaacggagc tctcaacag agctcggtt agctcgcag 
      tgggcgcaa cacacgtcg aaaatgtca accgaaacg 
      gttaaaacc gaaagtacg atgcacagt gtgcacaaa 
      gaaggcgac tcgctttta accatcaaa aacttcgac 
      caaaaaaca agcagtagc agtagcagc agctcgctg 
      gtttcaacg atagtaaat gccggacaa ctgagcatc 
      actgacgct gatgagaca caaccgcct gccgttcca 
      cagaaaacc aaaagaaaa gaacggcaa ccctcacct 
      tatgataat gtacctgat agtaattta ggaggcgaa 
      cttttagtc acttgcccc atgcaccaa tcccatcaa 
      agccttgcg agtagcact agtatgtca agtacgatg 
      ggtcaagac agcaataag cctccgcct ctcccaccc 
      aagaaaaaa cacataatg gcgtacatg gaaatgttc 
      ggcaactgc tcccacgcc aacgaacag gaattcatg 
      cgacattca gtgcacatg gtgcagtcg cactgggcc 
      caacctccg acccctggt ctgccaaca acccagtca 
      tgttcattc agtcacact tcacacagt tctagtcgt 
      tcatcacac actcaaatc caaattcta aacgtacca 
      ccacattcc acaatggac caaagagta tcatcgcct 
      ttgcattcc ccaaacccc atcataatg gaatcaaat 
      tctcctccc ccagctctc ccgcccaaa cagaggtct 
      aggaacagt tcggttaaa ccgtcgccg ccgcccacg 
      cccactgtt aacgaagtc aagccgcaa tcgccggtt 
      aaaacgcta ccggatttg ctagaacac accgccaat 
      tcggtgacg aagactgct gaagataag aatgaaatt 
      agtgaagtt aatcgctgt gatatagac ttaatggaa 
      gaagtgaac gttgatgat tggcttgtt ttgaagaaa 
      ccggaggaa gagggccct gatatcaga ggggggccc 
      attgatgcc ctcatcatc caggccacc aaggccacc 
      aaaaacgga gttttcatg tatcaagaa gcatttttg 
      acaacgtac cgcacgttc atcacccct ctcgaccta 
      ataacaaaa cttatacga agatacgat cacttttcg 
      ttcaaacct aacaacaag aaattgcga tcggaggcg 
      ttcgctttg atcgttcga gtggtgaat gacttgacc 
      gtttccgat ttggacgac caactccaa tgcaaactc 
      atgcacttc gtgcaacag ctcatctct tcgggcgaa 
      atcacctta gccaaagcc ctccgagtc aaacacttg 
      gaacggtac gaagccaaa caaatgagt ctgaaatac 
      accaatgtc gtgacgagt ctctcgctc actgcgcga 
      acttcgact ctgctcgac ttcaaatcg gagcaaatc 
      gccgaacaa atgactctg ctcgatgcc gatttgttt 
      atgaagatc gaaataccg gaagtgctg atttgggca 
      caggaacag aatgaggag aggagtccg aatttgacg 
      agattcact gaacatttt aataaaatg tcgtactgg 
      gcacgtacg aggatttta acagccgag gggaaagac 
      gtgcgggag aagtatttc ttgaagttt attaaaatt 
      atgaagcat ttgaggaaa attaataat tttaactcg 
      tatttggcg ctgctgtcg gcgctagat tcggccccc 
      attcggagg ttggagtgg cagaagcat gtgcaagaa 
      gggttgaag gagtattgt gctttaata gacagttcg 
      tcgagtttt agggcatat agggtggct ttggctgag 
      actcaaccc ccgtgcatc ccttacata gggttggtt 
      ctgcaagac ttaacattc gttcacata ggaaacagt 
      aacttatta cctgatggt acaataaac ttttcaaag 
      agatggcaa cagtacaat attgttgaa aatatgaaa 
      aagtttaag aaaggaatc tacactttc aaaaaacaa 
      gagcgcatt attcagttt ttccaaaac tttgatgat 
      ttcattggt gaggatgca atgtggcaa ttatcagag 
      tccataaaa ccccgtggt ggcaaaaaa gctacttcg 
      taa
    • >TC004461 RB
      atgatagaa agcgacagt cctattgct tcacctctg 
      gccgggtcc ggtcctggc ggccacaaa ccctccttc 
      aaaagcacc aaactcgct cgccgcgcc cgttcgttt 
      aaagacgac tttttgggc aaaatctcg caaatgcgc 
      agccccaca ggcccgggc ctccgctcg cacagcccc 
      aaggagcgg ccgtccaag tcggacatg gtcgagcac 
      gcgtattcg acgaccaaa gggcctcta caagagctg 
      gaaaacctc tggaagcag acccaggcc acgctaaaa 
      cacttccga gacgtcgtc tcgaagcaa aagctcgaa 
      atgctgccc ggaaatggc accatagtg ctcgacaca 
      gtgtggcaa ataaacctc gttgtgcga tcgtcaatc 
      aactcggat tcgagcaat gcgattttt tcggccacc 
      agccacatg taccagagt gtggccagg ctgatcaaa 
      ctgtgcgat gatgcgctg attgatgac aaaagcagc 
      gaattgacg acggagaat gtcaacgaa atcgtggcg 
      caagtggag gtggcggtg caggagctg atcaggttg 
      gtccaggag aagatcgcg caccaacaa atttcttat 
      aagacaaca ccaaggagt tcgtacgtt aacaccagc 
      ctagaaatg ccagcacaa cgaaattct ttacctgat 
      attcctctg acaccaagg gagaggcag ctgttggag 
      cagaattcg tgtgggccg ccgattgtg agaagcagt 
      cacagcacg gagtcgatc ttgagggac tccagtccg 
      cctcccaag ccaccgcta ccagatggt gttttgttt 
      aatataagc cactcggaa ccgagcagt tgtataaca 
      ccacctcct ttacctcca aagaagaaa aactacaaa 
      ctgcatcga ttgacagac gaatgttcg agtttggat 
      aactcccct attgcttct agtctagaa agagtctca 
      gttcggtcg aaatcaccc gaagactca atctccctg 
      ttatcagca agtgcgggc agtttagac tcggtccta 
      aaccactct cgtggcgag gaagacgac gaaattaaa 
      ggcataatg gagcggaca tgtgacagt gaagtcgat 
      aatcattca gctgatatt ttcggcgca aacgggtct 
      cacagttcc tgggaggag tcgtcatta agctcaaac 
      atatccggt caaatcacc gactacaat cgactatca 
      aacaccgac tcaggcttt atatcgtcg cagtcttac 
      cgatcgtcg agttactcc aaacggagc tctcaacag 
      agctcggtt agctcgcag tgggcgcaa cacacgtcg 
      aaaatgtca accgaaacg gttaaaacc gaaagtacg 
      atgcacagt gtgcacaaa gaaggcgac tcgctttta 
      accatcaaa aacttcgac caaaaaaca agcagtagc 
      agtagcagc agctcgctg gtttcaacg atagtaaat 
      gccggacaa ctgagcatc actgacgct gatgagaca 
      caaccgcct gccgttcca cagaaaacc aaaagaaaa 
      gaacggcaa ccctcacct tatgataat gtacctgat 
      agtaattta ggaggcgaa cttttagtc acttgcccc 
      atgcaccaa tcccatcaa agccttgcg agtagcact 
      agtatgtca agtacgatg ggtcaagac agcaataag 
      cctccgcct ctcccaccc aagaaaaaa cacataatg 
      gcgtacatg gaaatgttc ggcaactgc tcccacgcc 
      aacgaacag gaattcatg cgacattca gtgcacatg 
      gtgcagtcg cactgggcc caacctccg acccctggt 
      ctgccaaca acccagtca tgttcattc agtcacact 
      tcacacagt tctagtcgt tcatcacac actcaaatc 
      caaattcta aacgtacca ccacattcc acaatggac 
      caaagagta tcatcgcct ttgcattcc ccaaacccc 
      atcataatg gaatcaaat tctcctccc ccagctctc 
      ccgcccaaa cagaggtct aggaacagt tcggttaaa 
      ccgtcgccg ccgcccacg cccactgtt aacgaagtc 
      aagccgcaa tcgccggtt aaaacgcta ccggatttg 
      ctagaacac accgccaat tcggtgacg aagactgct 
      gaagataag aatgaaatt agtgaagtt aatcgctgt 
      gatatagac ttaatggaa gaagtgaac gttgatgat 
      tggcttgtt ttgaagaaa ccggaggaa gagggccct 
      gatatcaga ggggggccc attgatgcc ctcatcatc 
      caggccacc aaggccacc aaaaacgga gttttcatg 
      tatcaagaa gcatttttg acaacgtac cgcacgttc 
      atcacccct ctcgaccta ataacaaaa cttatacga 
      agatacgat cacttttcg ttcaaacct aacaacaag 
      aaattgcga tcggaggcg ttcgctttg atcgttcga 
      gtggtgaat gacttgacc gtttccgat ttggacgac 
      caactccaa tgcaaactc atgcacttc gtgcaacag 
      ctcatctct tcgggcgaa atcacctta gccaaagcc 
      ctccgagtc aaacacttg gaacggtac gaagccaaa 
      caaatgagt ctgaaatac accaatgtc gtgacgagt 
      ctctcgctc actgcgcga acttcgact ctgctcgac 
      ttcaaatcg gagcaaatc gccgaacaa atgactctg 
      ctcgatgcc gatttgttt atgaagatc gaaataccg 
      gaagtgctg atttgggca caggaacag aatgaggag 
      aggagtccg aatttgacg agattcact gaacatttt 
      aataaaatg tcgtactgg gcacgtacg aggatttta 
      acagccgag gggaaagac gtgcgggag aagtatttc 
      ttgaagttt attaaaatt atgaagcat ttgaggaaa 
      attaataat tttaactcg tatttggcg ctgctgtcg 
      gcgctagat tcggccccc attcggagg ttggagtgg 
      cagaagcat gtgcaagaa gggttgaag gagtattgt 
      gctttaata gacagttcg tcgagtttt agggcatat 
      agggtggct ttggctgag actcaaccc ccgtgcatc 
      ccttacata gggttggtt ctgcaagac ttaacattc 
      gttcacata ggaaacagt aacttatta cctgatggt 
      acaataaac ttttcaaag agatggcaa cagtacaat 
      attgttgaa aatatgaaa aagtttaag aaaggaatc 
      tacactttc aaaaaacaa gagcgcatt attcagttt 
      ttccaaaac tttgatgat ttcattggt gaggatgca 
      atgtggcaa ttatcagag tccataaaa ccccgtggt 
      ggcaaaaaa gctacttcg taa
    mRNA sequence
    • >TC004461 RA
      gaaggacaa acagaaatt aagttttga aattaaatg 
      tgccaccta atcatgata gaaagcgac agtcctatt 
      gcttcacct ctggccggg tccggtcct ggcggccac 
      aaaccctcc ttcaaaagc accaaactc gctcgccgc 
      gcccgttcg tttaaagac gactttttg ggcaaaatc 
      tcgcaaatg cgcagcccc acaggcccg ggcctccgc 
      tcgcacagc cccaaggag cggccgtcc aagtcggac 
      atggtcgag cacgcgtat tcgacgacc aaagggcct 
      ctacaagag ctggaaaac ctctggaag cagacccag 
      gccacgcta aaacacttc cgagacgtc gtctcgaag 
      caaaagctc gaaatgctg cccggaaat ggcaccata 
      gtgctcgac acagtgtgg caaataaac ctcgttgtg 
      cgatcgtca atcaactcg gattcgagc aatgcgatt 
      ttttcggcc accagccac atgtaccag agtgtggcc 
      aggctgatc aaactgtgc gatgatgcg ctgattgat 
      gacaaaagc agcgaattg acgacggag aatgtcaac 
      gaaatcgtg gcgcaagtg gaggtggcg gtgcaggag 
      ctgatcagg ttggtccag gagaagatc gcgcaccaa 
      caaatttct tataagaca acaccaagg agttcgtac 
      gttaacacc agcctagaa atgccagca caacgaaat 
      tctttacct gatattcct ctgacacca agggagagg 
      cagctgttg gagcagaat tcgtgtggg ccgccgatt 
      gtgagaagc agtcacagc acggagtcg atcttgagg 
      gactccagt ccgcctccc aagccaccg ctaccagat 
      ggccactcg gaaccgagc agttgtata acaccacct 
      cctttacct ccaaagaag aaaaactac aaactgcat 
      cgattgaca gacgaatgt tcgagtttg gataactcc 
      cctattgct tctagtcta gaaagagtc tcagttcgg 
      tcgaaatca cccgaagac tcaatctcc ctgttatca 
      gcaagtgcg ggcagttta gactcggtc ctaaaccac 
      tctcgtggc gaggaagac gacgaaatt aaaggcata 
      atggagcgg acatgtgac agtgaagtc gataatcat 
      tcagctgat attttcggc gcaaacggg tctcacagt 
      tcctgggag gagtcgtca ttaagctca aacatatcc 
      ggtcaaatc accgactac aatcgacta tcaaacacc 
      gactcaggc tttatatcg tcgcagtct taccgatcg 
      tcgagttac tccaaacgg agctctcaa cagagctcg 
      gttagctcg cagtgggcg caacacacg tcgaaaatg 
      tcaaccgaa acggttaaa accgaaagt acgatgcac 
      agtgtgcac aaagaaggc gactcgctt ttaaccatc 
      aaaaacttc gaccaaaaa acaagcagt agcagtagc 
      agcagctcg ctggtttca acgatagta aatgccgga 
      caactgagc atcactgac gctgatgag acacaaccg 
      cctgccgtt ccacagaaa accaaaaga aaagaacgg 
      caaccctca ccttatgat aatgtacct gatagtaat 
      ttaggaggc gaactttta gtcacttgc cccatgcac 
      caatcccat caaagcctt gcgagtagc actagtatg 
      tcaagtacg atgggtcaa gacagcaat aagcctccg 
      cctctccca cccaagaaa aaacacata atggcgtac 
      atggaaatg ttcggcaac tgctcccac gccaacgaa 
      caggaattc atgcgacat tcagtgcac atggtgcag 
      tcgcactgg gcccaacct ccgacccct ggtctgcca 
      acaacccag tcatgttca ttcagtcac acttcacac 
      agttctagt cgttcatca cacactcaa atccaaatt 
      ctaaacgta ccaccacat tccacaatg gaccaaaga 
      gtatcatcg cctttgcat tccccaaac cccatcata 
      atggaatca aattctcct cccccagct ctcccgccc 
      aaacagagg tctaggaac agttcggtt aaaccgtcg 
      ccgccgccc acgcccact gttaacgaa gtcaagccg 
      caatcgccg gttaaaacg ctaccggat ttgctagaa 
      cacaccgcc aattcggtg acgaagact gctgaagat 
      aagaatgaa attagtgaa gttaatcgc tgtgatata 
      gacttaatg gaagaagtg aacgttgat gattggctt 
      gttttgaag aaaccggag gaagagggc cctgatatc 
      agagggggg cccattgat gccctcatc atccaggcc 
      accaaggcc accaaaaac ggagttttc atgtatcaa 
      gaagcattt ttgacaacg taccgcacg ttcatcacc 
      cctctcgac ctaataaca aaacttata cgaagatac 
      gatcacttt tcgttcaaa cctaacaac aagaaattg 
      cgatcggag gcgttcgct ttgatcgtt cgagtggtg 
      aatgacttg accgtttcc gatttggac gaccaactc 
      caatgcaaa ctcatgcac ttcgtgcaa cagctcatc 
      tcttcgggc gaaatcacc ttagccaaa gccctccga 
      gtcaaacac ttggaacgg tacgaagcc aaacaaatg 
      agtctgaaa tacaccaat gtcgtgacg agtctctcg 
      ctcactgcg cgaacttcg actctgctc gacttcaaa 
      tcggagcaa atcgccgaa caaatgact ctgctcgat 
      gccgatttg tttatgaag atcgaaata ccggaagtg 
      ctgatttgg gcacaggaa cagaatgag gagaggagt 
      ccgaatttg acgagattc actgaacat tttaataaa 
      atgtcgtac tgggcacgt acgaggatt ttaacagcc 
      gaggggaaa gacgtgcgg gagaagtat ttcttgaag 
      tttattaaa attatgaag catttgagg aaaattaat 
      aattttaac tcgtatttg gcgctgctg tcggcgcta 
      gattcggcc cccattcgg aggttggag tggcagaag 
      catgtgcaa gaagggttg aaggagtat tgtgcttta 
      atagacagt tcgtcgagt tttagggca tatagggtg 
      gctttggct gagactcaa cccccgtgc atcccttac 
      atagggttg gttctgcaa gacttaaca ttcgttcac 
      ataggaaac agtaactta ttacctgat ggtacaata 
      aacttttca aagagatgg caacagtac aatattgtt 
      gaaaatatg aaaaagttt aagaaagga atctacact 
      ttcaaaaaa caagagcgc attattcag tttttccaa 
      aactttgat gatttcatt ggtgaggat gcaatgtgg 
      caattatca gagtccata aaaccccgt ggtggcaaa 
      aaagctact tcgtaagta gtggccatc ttagtgtaa 
      tagcaatac ttgtcgatt ttaacgcaa ttttgagct 
      ctatttaaa taaatattt atttataat tgtatattt 
      taaaaatca gtattttat gaaatgagc taggaggaa 
      ttttcgtga gacttcttc gtcctttac aaccaagag 
      ttttgtcgc gataaaacg taaaatgcg gtgctttca 
      atctctaac ttaggtctt aaaaaattc tatttattg 
      aaacaagga atttttatg caacgaatt tttgttaag 
      ctttcgcct aaaccaatc gattttttg cgaagtctt 
      tactctgtt cattatgaa tgctttatg cataagact 
      gagctgtac tgttttctg aatgttcta aggaatttt 
      tcgttataa gtggcattt gtaaaacgg actgaaata 
      aacactgta agttcaacg acataaaga cgatgttat 
      agcacttct tccatttat tgcaaccgc aaattgagt 
      gttagatta attaaactt aatagttat gtgtacgag 
      cattactgt tagagaaaa agttaacta ttagaaggc 
      ctaactaaa tataagcta acttaaatt aaggacagc 
      tgagtagac tgctctaac ccattaaat tatcaaacc 
      aaacaaaac tcgtggtga gtttattta tgttgcaat 
      attttttag accaattat acatgtgtt ttagatttg 
      caaatgtcg gagtgtatt ttttcaaaa atgtatcac 
      tttttagct caattgtat tatagatag atgtctatt 
      ctgttgaat cttctttta acagaaatg tgtaaggcg 
      attttcttc atgcatatt aacatatca taggtggaa 
      acatatgca gtgatacgg acgtactat tttcgattg 
      tgattcact cttatgatg cgtcttttg ttgcacatt 
      tctgtacaa ctgtgatgt caacttttt taactgttt 
      ttatttatc tttctttac acggtcacg ttatttatt 
      cagccgtgc ccaataaca cgttacaca ggcaagtta 
      tctttatta ggaacggcc agtgggtgt tactttgtg 
      tgccatttt attcattca ttttcatga tattaaatg 
      agttgtagt gattgtatg atgtacata aattgtgta 
      aatttgttt atagttttt tatttgttt ccgtaacat 
      gtttgtgta cttgctttt aaagtatgt at
    • >TC004461 RB
      gaaggacaa acagaaatt aagttttga aattaaatg 
      tgccaccta atcatgata gaaagcgac agtcctatt 
      gcttcacct ctggccggg tccggtcct ggcggccac 
      aaaccctcc ttcaaaagc accaaactc gctcgccgc 
      gcccgttcg tttaaagac gactttttg ggcaaaatc 
      tcgcaaatg cgcagcccc acaggcccg ggcctccgc 
      tcgcacagc cccaaggag cggccgtcc aagtcggac 
      atggtcgag cacgcgtat tcgacgacc aaagggcct 
      ctacaagag ctggaaaac ctctggaag cagacccag 
      gccacgcta aaacacttc cgagacgtc gtctcgaag 
      caaaagctc gaaatgctg cccggaaat ggcaccata 
      gtgctcgac acagtgtgg caaataaac ctcgttgtg 
      cgatcgtca atcaactcg gattcgagc aatgcgatt 
      ttttcggcc accagccac atgtaccag agtgtggcc 
      aggctgatc aaactgtgc gatgatgcg ctgattgat 
      gacaaaagc agcgaattg acgacggag aatgtcaac 
      gaaatcgtg gcgcaagtg gaggtggcg gtgcaggag 
      ctgatcagg ttggtccag gagaagatc gcgcaccaa 
      caaatttct tataagaca acaccaagg agttcgtac 
      gttaacacc agcctagaa atgccagca caacgaaat 
      tctttacct gatattcct ctgacacca agggagagg 
      cagctgttg gagcagaat tcgtgtggg ccgccgatt 
      gtgagaagc agtcacagc acggagtcg atcttgagg 
      gactccagt ccgcctccc aagccaccg ctaccagat 
      ggtgttttg tttaatata agccactcg gaaccgagc 
      agttgtata acaccacct cctttacct ccaaagaag 
      aaaaactac aaactgcat cgattgaca gacgaatgt 
      tcgagtttg gataactcc cctattgct tctagtcta 
      gaaagagtc tcagttcgg tcgaaatca cccgaagac 
      tcaatctcc ctgttatca gcaagtgcg ggcagttta 
      gactcggtc ctaaaccac tctcgtggc gaggaagac 
      gacgaaatt aaaggcata atggagcgg acatgtgac 
      agtgaagtc gataatcat tcagctgat attttcggc 
      gcaaacggg tctcacagt tcctgggag gagtcgtca 
      ttaagctca aacatatcc ggtcaaatc accgactac 
      aatcgacta tcaaacacc gactcaggc tttatatcg 
      tcgcagtct taccgatcg tcgagttac tccaaacgg 
      agctctcaa cagagctcg gttagctcg cagtgggcg 
      caacacacg tcgaaaatg tcaaccgaa acggttaaa 
      accgaaagt acgatgcac agtgtgcac aaagaaggc 
      gactcgctt ttaaccatc aaaaacttc gaccaaaaa 
      acaagcagt agcagtagc agcagctcg ctggtttca 
      acgatagta aatgccgga caactgagc atcactgac 
      gctgatgag acacaaccg cctgccgtt ccacagaaa 
      accaaaaga aaagaacgg caaccctca ccttatgat 
      aatgtacct gatagtaat ttaggaggc gaactttta 
      gtcacttgc cccatgcac caatcccat caaagcctt 
      gcgagtagc actagtatg tcaagtacg atgggtcaa 
      gacagcaat aagcctccg cctctccca cccaagaaa 
      aaacacata atggcgtac atggaaatg ttcggcaac 
      tgctcccac gccaacgaa caggaattc atgcgacat 
      tcagtgcac atggtgcag tcgcactgg gcccaacct 
      ccgacccct ggtctgcca acaacccag tcatgttca 
      ttcagtcac acttcacac agttctagt cgttcatca 
      cacactcaa atccaaatt ctaaacgta ccaccacat 
      tccacaatg gaccaaaga gtatcatcg cctttgcat 
      tccccaaac cccatcata atggaatca aattctcct 
      cccccagct ctcccgccc aaacagagg tctaggaac 
      agttcggtt aaaccgtcg ccgccgccc acgcccact 
      gttaacgaa gtcaagccg caatcgccg gttaaaacg 
      ctaccggat ttgctagaa cacaccgcc aattcggtg 
      acgaagact gctgaagat aagaatgaa attagtgaa 
      gttaatcgc tgtgatata gacttaatg gaagaagtg 
      aacgttgat gattggctt gttttgaag aaaccggag 
      gaagagggc cctgatatc agagggggg cccattgat 
      gccctcatc atccaggcc accaaggcc accaaaaac 
      ggagttttc atgtatcaa gaagcattt ttgacaacg 
      taccgcacg ttcatcacc cctctcgac ctaataaca 
      aaacttata cgaagatac gatcacttt tcgttcaaa 
      cctaacaac aagaaattg cgatcggag gcgttcgct 
      ttgatcgtt cgagtggtg aatgacttg accgtttcc 
      gatttggac gaccaactc caatgcaaa ctcatgcac 
      ttcgtgcaa cagctcatc tcttcgggc gaaatcacc 
      ttagccaaa gccctccga gtcaaacac ttggaacgg 
      tacgaagcc aaacaaatg agtctgaaa tacaccaat 
      gtcgtgacg agtctctcg ctcactgcg cgaacttcg 
      actctgctc gacttcaaa tcggagcaa atcgccgaa 
      caaatgact ctgctcgat gccgatttg tttatgaag 
      atcgaaata ccggaagtg ctgatttgg gcacaggaa 
      cagaatgag gagaggagt ccgaatttg acgagattc 
      actgaacat tttaataaa atgtcgtac tgggcacgt 
      acgaggatt ttaacagcc gaggggaaa gacgtgcgg 
      gagaagtat ttcttgaag tttattaaa attatgaag 
      catttgagg aaaattaat aattttaac tcgtatttg 
      gcgctgctg tcggcgcta gattcggcc cccattcgg 
      aggttggag tggcagaag catgtgcaa gaagggttg 
      aaggagtat tgtgcttta atagacagt tcgtcgagt 
      tttagggca tatagggtg gctttggct gagactcaa 
      cccccgtgc atcccttac atagggttg gttctgcaa 
      gacttaaca ttcgttcac ataggaaac agtaactta 
      ttacctgat ggtacaata aacttttca aagagatgg 
      caacagtac aatattgtt gaaaatatg aaaaagttt 
      aagaaagga atctacact ttcaaaaaa caagagcgc 
      attattcag tttttccaa aactttgat gatttcatt 
      ggtgaggat gcaatgtgg caattatca gagtccata 
      aaaccccgt ggtggcaaa aaagctact tcgtaagta 
      gtggccatc ttagtgtaa tagcaatac ttgtcgatt 
      ttaacgcaa ttttgagct ctatttaaa taaatattt 
      atttataat tgtatattt taaaaatca gtattttat 
      gaaatgagc taggaggaa ttttcgtga gacttcttc 
      gtcctttac aaccaagag ttttgtcgc gataaaacg 
      taaaatgcg gtgctttca atctctaac ttaggtctt 
      aaaaaattc tatttattg aaacaagga atttttatg 
      caacgaatt tttgttaag ctttcgcct aaaccaatc 
      gattttttg cgaagtctt tactctgtt cattatgaa 
      tgctttatg cataagact gagctgtac tgttttctg 
      aatgttcta aggaatttt tcgttataa gtggcattt 
      gtaaaacgg actgaaata aacactgta agttcaacg 
      acataaaga cgatgttat agcacttct tccatttat 
      tgcaaccgc aaattgagt gttagatta attaaactt 
      aatagttat gtgtacgag cattactgt tagagaaaa 
      agttaacta ttagaaggc ctaactaaa tataagcta 
      acttaaatt aaggacagc tgagtagac tgctctaac 
      ccattaaat tatcaaacc aaacaaaac tcgtggtga 
      gtttattta tgttgcaat attttttag accaattat 
      acatgtgtt ttagatttg caaatgtcg gagtgtatt 
      ttttcaaaa atgtatcac tttttagct caattgtat 
      tatagatag atgtctatt ctgttgaat cttctttta 
      acagaaatg tgtaaggcg attttcttc atgcatatt 
      aacatatca taggtggaa acatatgca gtgatacgg 
      acgtactat tttcgattg tgattcact cttatgatg 
      cgtcttttg ttgcacatt tctgtacaa ctgtgatgt 
      caacttttt taactgttt ttatttatc tttctttac 
      acggtcacg ttatttatt cagccgtgc ccaataaca 
      cgttacaca ggcaagtta tctttatta ggaacggcc 
      agtgggtgt tactttgtg tgccatttt attcattca 
      ttttcatga tattaaatg agttgtagt gattgtatg 
      atgtacata aattgtgta aatttgttt atagttttt 
      tatttgttt ccgtaacat gtttgtgta cttgctttt 
      aaagtatgt at
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