Status
Please wait ...
Gene information
General information
TC004457 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4785044..4791051
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004457
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03763

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    vitellarium shortened - Number of animals showing the phenotype: 4

    tropharium shortened - Number of animals showing the phenotype: 4
    egg chamber mostlynot present - Number of animals showing the phenotype: 4
    tropharium slightlyshape irregular - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004457 RA
      MASGIHTMS PQNSINLIS VYGKEEFNL HDFEENQNY 
      TQYSFSPEF YNYVNGSRS VIKKMPLNL TSHQKTVNN 
      NIQVKSLEQ RGNNSPQEC ESDENSLSH SPPDNKGTK 
      FKTAKETES EFNETCIYQ PNLYQPNVR CSKNSKYYY 
      RGGQKRFYN NLEETERKV HGLMRRFQS RAYKMKIDA 
      KPQILLNGG PWDPLNQAI WDVFTSKIQ KEETYTSKL 
      HLWKSIFLF FRMLNNYGL YLVGSTMSG FALEGSDID 
      ICLLTKPIS SEPRIDSLH HLDYLQHAL LENGLASEA 
      ELIMAKVPI LKFKNKETG FEIDLNCNN IVGIQNTRL 
      LYCYAQLDW RVRPLVVMV KIWAQRNHI NDAKNMTIS 
      SYSWTLMVI HYLQCGVFP AVLPCLHSL YPEEFNTLE 
      NRSLDVQGG VEGLKDFES ENTRCLGDL LIGFFHYYS 
      YFNYQHYAI SVRTGSRIP IEICKQVKS PKNDPHQWK 
      FLCIEEPFD LSNTARSVF DLEIFKHIK QIISASYKE 
      LARNKQLSN ILPVAQLNG QR
    CDS sequence
    • >TC004457 RA
      atggcatcg ggaattcac acaatgtcg cctcagaat 
      agcataaac ttgatcagt gtctacggt aaagaagag 
      ttcaatctg cacgatttt gaggaaaac cagaattac 
      acgcaatac agcttcagt cccgagttc tacaattac 
      gtgaatgga tcacgatct gtaataaag aagatgcca 
      ttgaatttg acctcacac caaaaaacc gtcaacaac 
      aacatccaa gtgaaaagt ttagagcag cgaggtaat 
      aactctcca caggagtgc gagagcgac gagaacagc 
      ctctcacat tcaccccct gacaataaa ggaacgaag 
      tttaagacg gctaaagaa actgaaagt gaattcaac 
      gagacgtgc atttatcag ccaaacttg taccaacca 
      aacgttcgc tgcagcaaa aacagcaag tattactac 
      cgcggtggc cagaagaga ttttacaac aatctggaa 
      gagactgag agaaaagtg cacgggtta atgcgccgt 
      ttccagtca agggcgtac aaaatgaaa atcgatgcc 
      aagccccaa attttgcta aatgggggc ccttgggac 
      cccctgaac caagcaatc tgggacgtg tttacgagc 
      aaaatacaa aaggaagaa acctacaca agcaaactc 
      cacttgtgg aaatcaata tttttattt tttcggatg 
      ttaaataac tatggattg tatttagtg gggtccacg 
      atgtcgggc ttcgccctc gagggcagc gacatcgat 
      atttgtctt ttaacgaaa cccattagt agcgagccg 
      aggattgac tcgttacac catttggat tatttgcag 
      cacgctctg ctggaaaat ggtctggca agcgaagct 
      gaactaatt atggcgaag gttccgatt ctaaaattc 
      aaaaataaa gaaaccgga tttgaaatt gatttgaat 
      tgtaataac attgtgggg attcaaaat acaagactg 
      ttgtattgt tatgcccaa cttgactgg agagtgcga 
      cctctggtg gttatggtg aagatttgg gcgcaaaga 
      aaccacatt aatgatgcc aaaaatatg accatttcg 
      agctactcg tggacgttg atggtcata cactacttg 
      cagtgtggg gtgttccca gcagtgctc ccttgtctc 
      cacagcttg taccccgaa gagttcaac acgctggaa 
      aaccggtcg ttggacgtg cagggagga gtggaaggt 
      ttgaaagac tttgaatct gaaaacaca cgctgtctc 
      ggtgattta ctgatcggt tttttccac tattactcc 
      tattttaac tatcaacat tatgctatt tcagtacgt 
      accggttca cgtataccg attgaaatc tgtaaacag 
      gtaaaaagt cctaaaaac gacccccac caatggaaa 
      ttcttgtgt attgaggaa cctttcgat ctctccaat 
      actgccaga tccgtgttt gatttggaa attttcaaa 
      catatcaaa caaatcatt tcggcctcg tataaagaa 
      ctggcgcgc aacaaacaa ctgagtaat atacttccg 
      gtagcgcag ttgaacggt caaagataa
    mRNA sequence
    • >TC004457 RA
      ttcctcgtc tgggaatga accaaataa agaaacaga 
      acttttaag ccaaaaaga cgctcttgc ggacgtcct 
      gtggaagat atttaaaca gaaggagga agggcggga 
      gagaagaca ccgggaaaa aatggaact catcaaatc 
      tctattgca aaataattg ataaataaa ttgtacttt 
      ttaaaccga cttggtgtt tttgcgttg ttgtgttgg 
      cagttgaaa aatgagagc gcaggcgtt atttggcgg 
      caataaaca gtaaaataa attgtaaaa gtgtattat 
      ttattgtgt gcttattgt tataagtta tttcatgtt 
      gaaattcac actatttac tttcccaga atggatgtg 
      taattcgag tgtaaactt tgggggttt ttggtaaaa 
      tggcatcgg gaattcaca caatgtcgc ctcagaata 
      gcataaact tgatcagtg tctacggta aagaagagt 
      tcaatctgc acgattttg aggaaaacc agaattaca 
      cgcaataca gcttcagtc ccgagttct acaattacg 
      tgaatggat cacgatctg taataaaga agatgccat 
      tgaatttga cctcacacc aaaaaaccg tcaacaaca 
      acatccaag tgaaaagtt tagagcagc gaggtaata 
      actctccac aggagtgcg agagcgacg agaacagcc 
      tctcacatt caccccctg acaataaag gaacgaagt 
      ttaagacgg ctaaagaaa ctgaaagtg aattcaacg 
      agacgtgca tttatcagc caaacttgt accaaccaa 
      acgttcgct gcagcaaaa acagcaagt attactacc 
      gcggtggcc agaagagat tttacaaca atctggaag 
      agactgaga gaaaagtgc acgggttaa tgcgccgtt 
      tccagtcaa gggcgtaca aaatgaaaa tcgatgcca 
      agccccaaa ttttgctaa atgggggcc cttgggacc 
      ccctgaacc aagcaatct gggacgtgt ttacgagca 
      aaatacaaa aggaagaaa cctacacaa gcaaactcc 
      acttgtgga aatcaatat ttttatttt ttcggatgt 
      taaataact atggattgt atttagtgg ggtccacga 
      tgtcgggct tcgccctcg agggcagcg acatcgata 
      tttgtcttt taacgaaac ccattagta gcgagccga 
      ggattgact cgttacacc atttggatt atttgcagc 
      acgctctgc tggaaaatg gtctggcaa gcgaagctg 
      aactaatta tggcgaagg ttccgattc taaaattca 
      aaaataaag aaaccggat ttgaaattg atttgaatt 
      gtaataaca ttgtgggga ttcaaaata caagactgt 
      tgtattgtt atgcccaac ttgactgga gagtgcgac 
      ctctggtgg ttatggtga agatttggg cgcaaagaa 
      accacatta atgatgcca aaaatatga ccatttcga 
      gctactcgt ggacgttga tggtcatac actacttgc 
      agtgtgggg tgttcccag cagtgctcc cttgtctcc 
      acagcttgt accccgaag agttcaaca cgctggaaa 
      accggtcgt tggacgtgc agggaggag tggaaggtt 
      tgaaagact ttgaatctg aaaacacac gctgtctcg 
      gtgatttac tgatcggtt ttttccact attactcct 
      attttaact atcaacatt atgctattt cagtacgta 
      ccggttcac gtataccga ttgaaatct gtaaacagg 
      taaaaagtc ctaaaaacg acccccacc aatggaaat 
      tcttgtgta ttgaggaac ctttcgatc tctccaata 
      ctgccagat ccgtgtttg atttggaaa ttttcaaac 
      atatcaaac aaatcattt cggcctcgt ataaagaac 
      tggcgcgca acaaacaac tgagtaata tacttccgg 
      tagcgcagt tgaacggtc aaagataat cgagttggt 
      tgtaaaaat gatgggatt ttgtggcaa tagtggcct 
      tctgataca ttccgtaac tttttaagc aattgaatt 
      tgttctaag tataagttg taagagttt cttcggtgt 
      tgagtccgg tttattttt tccaagggg attcttatt 
      gttgttact gtaaactct ctgtgattt tttttcgct 
      ttatataag aaaaaatat accaaaaaa atacataaa 
      attgccaca atgaaacat ttttaaaaa ctcattgtg 
      gacgaaaaa aagtattat tttgtacag gttcgtgtt 
      tatttttat acttcgttt gtaaattat aagtaaata 
      tcgctctct catattttt acgcttaat gttacgaga 
      ggttaactt aatcgtgct cgtaatttg gtaaaacat 
      acatatcaa atacgggtc tagagactt agccttttt 
      cgaaaaata aaaaaataa aataaaaaa gcaacgatt 
      acacatagt tcaccaccg tctgagata caaaccaaa 
      aatgatttg ccataagta cgaatcaca agtcgatgt 
      taatttgag acttataaa tattgtgat ttatcgtct 
      gatctcagt actacttta atgtaatag tcacaatgt 
      gccaaatgt taattaagg ttaattcta aacttgtaa 
      ttagacaat atttaacat cggatacaa taattgtaa 
      acaataact tgaaaactg tttgtttac gtaggaatt 
      gtagttgat ctttatctt caggaactg tgaacattt 
      tccactagt taagacagt gtttgtaag atttttgaa 
      tgttccatt ttgtaaagc ggagattca gtgttgaac 
      atcaaatta tctttaagc tgttgttca gttcagtca 
      atataattc atttcataa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY