Status
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Gene information
General information
TC032023
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004454,TC004453
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 2708861..2714168
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032023
    iBeetle screen iB_03762
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    labium not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032023 RA
      MAHMTKKRH VLKIKGNNK TNAKESNKP DKSSVWRVI 
      APFVTVPLK KPQKEVEDE QFSFYLKKK PAVLLRDLS 
      TFMSDSVDL PSLFHETAD VLQKVTKAS WVTLYLVDR 
      ATNEIYVSH PNTTRERHK VKWKIEEQS TVATYVAKK 
      MEYVVVEDV LLDIRFPEG IGYDASTIP RSVMCIPIV 
      TPDGDCFAV IEFVKEYHL KCFVQDDLK ICVMVTAWM 
      GVAIHQNQE RLALKRQQE LNDYLLDLV KCYFEDHVL 
      MEKLMSEVV KFAKITLGA ERGSFFIID KDSDEMIAH 
      LFDEGGQTE GPGRKVRFS KERGIAGLV ARTGQTVNI 
      KDAYNDSRF NKEIDQRTG FITRSILCM PILGKNGVL 
      GVVQVVNKK NGGCFSSND ENLFKTFSL YCSMAVHYT 
      NIHSEIKKQ TYLNKINLK VIKLQQVPC LHDLTNFTD 
      RSYVNIPVE FDSFTWSIN NSDELVYLP QYILYIMTQ 
      LISVSEINL NVMKKFILT VRKFYRDLP YHNFEHAFT 
      VCHCMYLVL KRNLDQFSK LEVKALLIA ALCHDIDHP 
      GFTNNFLQI IKDDIAILY EAPALENHH WEVTKMILN 
      DHDIFPKMS GQERELFHS EIQKAILAT DLSTFFQTR 
      VTLAKLYNA KTFDRYNPS HRVKMKAII INACDFSGL 
      SKPFLAAKR IVDQLYRMV DNWVEFGLS LIAGEFYKQ 
      GDLEKELGQ CPLSVMDRG KENCIPEDQ IRFLKRNAI 
      ILQNLSNIC MPILGKNGV LGVVQVVNK KNGGCFSSN 
      DENLFKTFS LYCSMAVHY TNIHSEIKK QTYLNKINL 
      KVIKLQQVP CLHDLTNFT DRSYVNIPV EFDSFTWSI 
      NNSDELVYL PQYILYIMT QLISVSEIN LNVMKKFIL 
      TVRKFYRDL PYHNFEHAF TVCHCMYLV LKRNLDQFS 
      KLEVKALLI AALCHDIDH PGFTNNFLQ IIKDDIAIL 
      YEAPALENH HWEVTKMIQ NDHDIFPKM SGQERELFH 
      SEIQKAILA TDLSTFFQT RVTLAKLYN AKTFDRYNP 
      SHRVKMKAI IINACDFSG LSKPFLAAK RIVDQLYRE 
      FYKQGDLEK ELGQCPLSV MDRGKENCI PEDQIRFLK 
      VVVIPCVEI IRVILPNCR ELYNGTLRL RQTWEDIIK 
      NTGRKIWRQ DDSIVNPTY I
    CDS sequence
    • >TC032023 RA
      atggcgcac atgacgaag aaaaggcat gtattaaaa 
      attaaaggg aacaataaa accaacgcc aaagagagc 
      aacaagccc gacaaatcg tcagtatgg cgcgtaata 
      gccccgttc gtgaccgtc ccactgaag aagccccag 
      aaggaggtg gaagacgaa cagttcagt ttctacttg 
      aagaaaaag ccggctgtt cttctgcgc gacttgtca 
      acgtttatg tcagatagt gtcgacttg ccgtcactc 
      tttcacgaa actgccgat gttctacaa aaggttact 
      aaagcttcg tgggttact ctgtacctg gtcgaccgc 
      gccacgaac gagatctac gtcagccac ccaaacacc 
      acgcgggag agacacaaa gtcaaatgg aaaatagag 
      gagcagtcg accgttgcc acctacgtg gccaagaaa 
      atggaatac gttgtggtg gaggacgtg ctccttgat 
      atccgattt ccggaagga atcggttat gacgctagc 
      accatcccg aggtccgtc atgtgcatc cccattgtg 
      accccggac ggcgactgc ttcgctgtg atcgagttc 
      gtgaaggag taccacttg aagtgcttc gtccaggac 
      gatctcaag atctgcgtc atggtcacg gcctggatg 
      ggggtggcc atccaccag aaccaggag cgtctggct 
      ttgaagcgg cagcaggaa ctcaacgat tacctcctg 
      gacctggtc aagtgctac ttcgaggac cacgtcctc 
      atggagaaa ctcatgagc gaggtggtg aagttcgcg 
      aagatcacc ctcggcgcc gaacgaggc tctttcttc 
      atcatcgac aaagatagt gacgaaatg atcgcgcat 
      ttgttcgac gaaggcgga cagactgaa gggccagga 
      cggaaagtc cggttctcc aaagaacgg gggattgcc 
      ggcctagtg gcccggact gggcaaaca gtcaacatc 
      aaagacgct tacaacgac agcaggttc aacaaggag 
      attgaccaa cggacgggg ttcatcacc aggtcgata 
      ctctgcatg cccattttg ggcaagaac ggggtcttg 
      ggagtcgtc caagtcgtc aacaagaaa aacggaggg 
      tgtttctca agcaacgac gagaacctt ttcaaaact 
      ttctccctg tattgctcc atggcggtg cactacacc 
      aacatccat tccgaaatc aagaaacag acctacctg 
      aacaaaatc aacctgaaa gtgatcaag ttgcagcaa 
      gtgccctgc ctgcacgac ttgaccaac ttcaccgac 
      aggtcctac gtgaacata ccagtggag ttcgacagc 
      ttcacctgg agtatcaac aattcggac gaactcgtg 
      tacctaccg cagtacata ttgtacata atgacccaa 
      ttgatcagc gtttccgaa attaatctc aacgtaatg 
      aagaaattt attttgaca gttcgcaaa ttctaccga 
      gacttgcct taccacaac ttcgaacat gccttcacc 
      gtgtgccat tgcatgtac ctcgtgcta aagcgcaat 
      ttggaccag tttagcaaa ttggaagtc aaagccctg 
      ctcatcgcc gccctctgc cacgacata gaccacccc 
      gggttcacc aacaatttc ctccaaatc atcaaagac 
      gacatcgcc atactttac gaagcccca gccttggag 
      aaccaccac tgggaggtg accaagatg atcctgaac 
      gaccacgac atcttcccc aagatgagc gggcaggag 
      cgggagttg ttccacagc gagatacag aaggctatt 
      ctagccaca gacctgtcc acttttttc cagactaga 
      gtcaccctc gccaagttg tacaacgcg aaaactttc 
      gatcgatat aatccgagt catagggtg aagatgaag 
      gcgattatc atcaatgct tgtgatttt tcggggttg 
      tcgaagccg tttctggcc gcgaaaagg atagtggat 
      cagctttat cgtatggtg gataactgg gttgagttt 
      gggttaagt ttgattgca ggcgagttc tacaaacaa 
      ggtgatttg gaaaaggag ctggggcaa tgtccgttg 
      tcggttatg gacagaggg aaggagaat tgcattccg 
      gaggatcag atacggttt ttgaagaga aacgcaatt 
      attttgcaa aatttgtca aatatctgc atgcccatt 
      ttgggcaag aacggggtc ctgggagtg gttcaagtc 
      gttaataag aaaaacgga gggtgtttc tcaagcaac 
      gacgagaac cttttcaaa acgttctcc ctgtattgc 
      tccatggcg gtgcactac accaacatc cattccgaa 
      atcaagaaa cagacctac ctgaacaaa atcaacctg 
      aaagtgatc aagttgcag caagtgccc tgcctgcac 
      gacctgacg aacttcacc gacaggtcc tacgtgaac 
      ataccagtg gagttcgac agcttcacc tggagtatc 
      aacaattcg gacgaactc gtgtaccta ccgcagtac 
      atattgtac ataatgacc caattgatc agcgtttcc 
      gaaattaat ctcaacgta atgaagaaa tttattttg 
      acagttcgc aaattctac cgagacttg ccttaccac 
      aacttcgaa catgccttc accgtgtgc cattgcatg 
      tacctcgtg ctaaagcgc aatttggac cagtttagc 
      aaattggaa gtcaaagcc ctgctcatc gccgccctc 
      tgccacgac atagaccac cccgggttc accaacaat 
      ttcctccaa atcatcaaa gacgacatc gccatactt 
      tacgaagcc ccagccttg gagaaccac cactgggag 
      gtgaccaag atgatccag aacgaccac gacatcttc 
      cccaagatg agcgggcag gagagggag ttgttccac 
      agcgagata cagaaggct attctggcc acagacctg 
      tccactttt ttccagact agagtcacc ctcgccaag 
      ttgtacaat gcgaaaact ttcgatcga tataatccg 
      agtcatagg gtgaagatg aaggcgatt atcatcaat 
      gcttgtgat ttttcgggg ttgtcgaag ccgtttctg 
      gccgcgaaa aggatagtg gatcagctt tatcgcgag 
      ttctacaaa caaggggat ttggaaaag gagctgggg 
      caatgtccg ttgtcggtt atggacaga gggaaggag 
      aattgcatt ccggaggat cagatacgg tttttgaag 
      gttgtagtc ataccgtgc gtcgaaatt attcgggtg 
      attctgcca aattgccgc gagttgtac aatggaact 
      ttgagatta cgccaaacc tgggaggat attatcaaa 
      aataccggg cgcaaaatt tggcgacaa gacgattcc 
      attgttaat cctacttat atttaa
    mRNA sequence
    • >TC032023 RA
      tgtttttgc cccattcgg tggcaaatt taattataa 
      gtagattaa tttttttgt cccgttgca aaattatgt 
      aaaaatggc gcacatgac gaagaaaag gcatgtatt 
      aaaaattaa agggaacaa taaaaccaa cgccaaaga 
      gagcaacaa gcccgacaa atcgtcagt atggcgcgt 
      aatagcccc gttcgtgac cgtcccact gaagaagcc 
      ccagaagga ggtggaaga cgaacagtt cagtttcta 
      cttgaagaa aaagccggc tgttcttct gcgcgactt 
      gtcaacgtt tatgtcaga tagtgtcga cttgccgtc 
      actctttca cgaaactgc cgatgttct acaaaaggt 
      tactaaagc ttcgtgggt tactctgta cctggtcga 
      ccgcgccac gaacgagat ctacgtcag ccacccaaa 
      caccacgcg ggagagaca caaagtcaa atggaaaat 
      agaggagca gtcgaccgt tgccaccta cgtggccaa 
      gaaaatgga atacgttgt ggtggagga cgtgctcct 
      tgatatccg atttccgga aggaatcgg ttatgacgc 
      tagcaccat cccgaggtc cgtcatgtg catccccat 
      tgtgacccc ggacggcga ctgcttcgc tgtgatcga 
      gttcgtgaa ggagtacca cttgaagtg cttcgtcca 
      ggacgatct caagatctg cgtcatggt cacggcctg 
      gatgggggt ggccatcca ccagaacca ggagcgtct 
      ggctttgaa gcggcagca ggaactcaa cgattacct 
      cctggacct ggtcaagtg ctacttcga ggaccacgt 
      cctcatgga gaaactcat gagcgaggt ggtgaagtt 
      cgcgaagat caccctcgg cgccgaacg aggctcttt 
      cttcatcat cgacaaaga tagtgacga aatgatcgc 
      gcatttgtt cgacgaagg cggacagac tgaagggcc 
      aggacggaa agtccggtt ctccaaaga acgggggat 
      tgccggcct agtggcccg gactgggca aacagtcaa 
      catcaaaga cgcttacaa cgacagcag gttcaacaa 
      ggagattga ccaacggac ggggttcat caccaggtc 
      gatactctg catgcccat tttgggcaa gaacggggt 
      cttgggagt cgtccaagt cgtcaacaa gaaaaacgg 
      agggtgttt ctcaagcaa cgacgagaa ccttttcaa 
      aactttctc cctgtattg ctccatggc ggtgcacta 
      caccaacat ccattccga aatcaagaa acagaccta 
      cctgaacaa aatcaacct gaaagtgat caagttgca 
      gcaagtgcc ctgcctgca cgacttgac caacttcac 
      cgacaggtc ctacgtgaa cataccagt ggagttcga 
      cagcttcac ctggagtat caacaattc ggacgaact 
      cgtgtacct accgcagta catattgta cataatgac 
      ccaattgat cagcgtttc cgaaattaa tctcaacgt 
      aatgaagaa atttatttt gacagttcg caaattcta 
      ccgagactt gccttacca caacttcga acatgcctt 
      caccgtgtg ccattgcat gtacctcgt gctaaagcg 
      caatttgga ccagtttag caaattgga agtcaaagc 
      cctgctcat cgccgccct ctgccacga catagacca 
      ccccgggtt caccaacaa tttcctcca aatcatcaa 
      agacgacat cgccatact ttacgaagc cccagcctt 
      ggagaacca ccactggga ggtgaccaa gatgatcct 
      gaacgacca cgacatctt ccccaagat gagcgggca 
      ggagcggga gttgttcca cagcgagat acagaaggc 
      tattctagc cacagacct gtccacttt tttccagac 
      tagagtcac cctcgccaa gttgtacaa cgcgaaaac 
      tttcgatcg atataatcc gagtcatag ggtgaagat 
      gaaggcgat tatcatcaa tgcttgtga tttttcggg 
      gttgtcgaa gccgtttct ggccgcgaa aaggatagt 
      ggatcagct ttatcgtat ggtggataa ctgggttga 
      gtttgggtt aagtttgat tgcaggcga gttctacaa 
      acaaggtga tttggaaaa ggagctggg gcaatgtcc 
      gttgtcggt tatggacag agggaagga gaattgcat 
      tccggagga tcagatacg gtttttgaa gagaaacgc 
      aattatttt gcaaaattt gtcaaatat ctgcatgcc 
      cattttggg caagaacgg ggtcctggg agtggttca 
      agtcgttaa taagaaaaa cggagggtg tttctcaag 
      caacgacga gaacctttt caaaacgtt ctccctgta 
      ttgctccat ggcggtgca ctacaccaa catccattc 
      cgaaatcaa gaaacagac ctacctgaa caaaatcaa 
      cctgaaagt gatcaagtt gcagcaagt gccctgcct 
      gcacgacct gacgaactt caccgacag gtcctacgt 
      gaacatacc agtggagtt cgacagctt cacctggag 
      tatcaacaa ttcggacga actcgtgta cctaccgca 
      gtacatatt gtacataat gacccaatt gatcagcgt 
      ttccgaaat taatctcaa cgtaatgaa gaaatttat 
      tttgacagt tcgcaaatt ctaccgaga cttgcctta 
      ccacaactt cgaacatgc cttcaccgt gtgccattg 
      catgtacct cgtgctaaa gcgcaattt ggaccagtt 
      tagcaaatt ggaagtcaa agccctgct catcgccgc 
      cctctgcca cgacataga ccaccccgg gttcaccaa 
      caatttcct ccaaatcat caaagacga catcgccat 
      actttacga agccccagc cttggagaa ccaccactg 
      ggaggtgac caagatgat ccagaacga ccacgacat 
      cttccccaa gatgagcgg gcaggagag ggagttgtt 
      ccacagcga gatacagaa ggctattct ggccacaga 
      cctgtccac ttttttcca gactagagt caccctcgc 
      caagttgta caatgcgaa aactttcga tcgatataa 
      tccgagtca tagggtgaa gatgaaggc gattatcat 
      caatgcttg tgatttttc ggggttgtc gaagccgtt 
      tctggccgc gaaaaggat agtggatca gctttatcg 
      cgagttcta caaacaagg ggatttgga aaaggagct 
      ggggcaatg tccgttgtc ggttatgga cagagggaa 
      ggagaattg cattccgga ggatcagat acggttttt 
      gaaggttgt agtcatacc gtgcgtcga aattattcg 
      ggtgattct gccaaattg ccgcgagtt gtacaatgg 
      aactttgag attacgcca aacctggga ggatattat 
      caaaaatac cgggcgcaa aatttggcg acaagacga 
      ttccattgt taatcctac ttatattta ataaaataa 
      tgttaaaaa atatttttt ctttcactt gtcgtttcc 
      gttaattaa cagtacttc catttcaaa aaaatccac 
      actaaataa ctttgcata gg