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Gene information
General information
TC004452 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4806890..4810141
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004452
    iBeetle screen iB_03761

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004452 RA
      MRQILWLIT FCTILKFAF CEIICNDPK GSCGCLGRR 
VLCSTAKTD DIIIDISKG IIDMECPRE LKQLHFEKP 
PNVTIDSDI SKFTMRYCP LPPNNFREI FAWFSLQNV 
ETFEFYASE LNACTLTNK YFQSLENLK QLILSDNAI 
DDLDENFFT NMPQVKLLD LKNNRIKLT KSTFKNLQF 
LQHLDLSSN NIKFVPHGA FQELETLTT LNLFDNQLT 
KIDDFTFAG LSNLQSLEL SANKIQTIS ENAFATLKN 
LTRINLSNN FLKTLPGGL FQGNRNLKT LRLKHNIGL 
QLPGLVFAN LFLTEVDLT KCRLGEIPE NVFENTTTL 
KVVELGGND LEDLPENVF KGLTNLGKI SLQHNKIKS 
ISHLFKGLE RITLLQLQK NSIEKIESE AFADLINLE 
KINLRGNRI KQINPLVFS RNHKLKTVV LADNEIVDK 
FEEAITSTQ TNNLIRWNL RHNRIQQIK LANWKNVSI 
TLDYNPLIC NCLNYNLLN HIRNQTRSN NLTITAKNV 
KCEGSQVFE NLELSSLTC SLDEISKNY LCPKNCSCI 
WRPFDASVV INCSNKSLV KYPQVDLSQ LTYVYNDIE 
LHLENNKLE SGPERRFGF LNITKLFLS NNTIRSIDW 
VPPRLKSLH LDKNRISKI PPKVIEVLK QTTLVNLTL 
NDNPWMCDC GAEILAQFL RTFPNFSTN IYCANTTLR 
LIDMKKEDF CKSQTVLYL TTLTLSLLL ISALLAIFY 
YRNQETINF WLLSHNLCW FSEENRDKD KIYDVFISY 
SHKDEHFVE ALLSFLEKG PNSFKTCVH YRDWTPGEP 
IVSQIINSV VNSRRTLVV LSRNFLESV WGKMEFKTA 
HRQALSDGR NRVVVVWYG DVEVEKLDE ELKVYLRTN 
TFVKWGDKF FWEKLKIAL RRTVKWGSK MGVQEEEDM 
LIV
    CDS sequence
    • >TC004452 RA
      atgcggcag atattatgg ttgatcacg ttttgcaca 
attttgaaa tttgccttt tgcgaaatt atttgcaac 
gatcccaaa ggaagttgt ggatgttta ggtcgtcgc 
gtattatgt tctacggca aaaaccgac gatatcatc 
attgatatc agcaaagga ataattgac atggagtgt 
cccagagaa ttgaaacaa ctccacttt gaaaagcct 
ccaaacgtg actattgac agcgacatt tcaaaattt 
acaatgcgg tattgtcca ctaccaccg aacaacttc 
agagaaatt ttcgcctgg ttttcgcta caaaatgtg 
gaaacattc gaattttac gcctcggag ctgaacgcc 
tgcacctta acaaacaaa tattttcaa tcattggaa 
aatctgaaa cagttaatt ctctctgat aacgcaatt 
gatgatttg gacgaaaac tttttcaca aacatgcca 
caagtgaaa cttctagac ctcaaaaat aatcgcatc 
aaattgaca aaatcgact ttcaaaaat ttacagttt 
ttacaacac ttggatttg tcgtccaat aacataaaa 
tttgtacct cacggagcc tttcaagaa ctcgaaacg 
ctgacaact ttgaatttg ttcgacaac caactgacc 
aaaatcgac gattttaca tttgccgga ctttcaaac 
ctgcaatcg ctcgaatta agcgccaac aaaatacaa 
acaataagt gaaaacgca ttcgccact cttaaaaat 
ctgacacgg attaatctt tcaaacaac ttccttaaa 
acactgcct ggggggtta ttccaaggc aacagaaac 
ctaaaaacg ctcagactt aaacacaat attgggtta 
caactcccg ggtttagtt ttcgcaaat ttattccta 
accgaagtg gatttgacg aagtgtcgt ttaggcgag 
atccccgaa aatgttttt gagaacacg acaacgttg 
aaagttgtc gagttggga ggaaatgat ttggaagac 
ttgccagag aacgtgttc aaaggcttg acgaatttg 
ggcaaaatt tctttgcaa cacaacaag attaagtca 
atttcacac ttgttcaaa ggacttgag cgaattact 
ttgctgcag ctacaaaaa aactcaatt gagaaaatt 
gagagcgaa gctttcgcc gatttgata aatctggaa 
aaaattaac ttgcggggg aatagaatt aaacaaatt 
aacccgctt gttttttca aggaatcat aaactaaaa 
accgtcgtt ttggctgat aatgagata gtggataag 
tttgaggag gcaataact tcaactcaa acaaataac 
ttgattcgc tggaatttg cgccacaac cggatccag 
caaataaag ctcgcgaac tggaaaaac gtatcaata 
acactcgat tacaatcca cttatttgt aactgtctc 
aattacaat ttactgaac catatcaga aaccagaca 
agaagtaat aacttgaca ataactgca aaaaatgtt 
aaatgtgag ggctctcaa gtgtttgag aatcttgaa 
ctgtcgagt ctcacatgt tcactggac gaaatttcc 
aaaaattat ttgtgtcct aaaaactgc tcgtgcatt 
tggagacct ttcgacgct tctgtcgta atcaactgt 
tcaaataaa tcgctcgtt aagtaccca caagtggac 
ctaagtcag ttaacttac gtttacaac gacattgaa 
ttacacctt gaaaataat aaattggaa tcagggcct 
gagcgccgt tttggtttt ctcaatatt acaaaattg 
tttctttca aacaacaca atcaggtca attgattgg 
gtgccacca aggcttaag tcattacat ttggacaaa 
aaccgcatc agcaaaatc cctcccaaa gtcattgag 
gttttaaaa cagacaact ttggtaaat ttgacttta 
aacgacaat ccttggatg tgcgattgt ggggctgaa 
attttggcc caatttttg cgtacattt ccgaacttt 
tccactaac atttattgc gctaacaca acccttcgc 
ctaattgat atgaaaaag gaggacttt tgcaaaagc 
cagactgtc ctttattta acaacacta accctctca 
ctccttctc atttcagcc cttttggcc attttctac 
taccggaac caggaaact atcaacttc tggcttctt 
tcccacaat ttgtgttgg tttagtgaa gaaaatcgc 
gataaagac aagatctac gacgttttt atcagttat 
tcacataaa gacgaacat tttgtcgag gctctctta 
tcgttcctt gaaaaaggt cccaattct tttaaaact 
tgcgtacat tatcgtgat tggacgcct ggagagcct 
atcgtgagc caaatcatc aattccgtg gtgaattcg 
cgccgaact ttggtggtg ctttcaagg aatttcctc 
gaaagtgtt tgggggaaa atggagttt aagacggcg 
cacaggcaa gctttgagc gatggaagg aatcgggtt 
gtggtagtt tggtatgga gatgttgaa gttgagaag 
ttggatgag gagttgaag gtgtatttg cggactaat 
acttttgtt aagtggggg gataagttt ttttgggaa 
aagctgaag atagcgttg cgacggact gtgaaatgg 
gggtccaaa atgggggta caagaggag gaagatatg 
ttaatagtg tag
    mRNA sequence
    • >TC004452 RA
      tttttgata gggtatcag ttttcgcga aaaaggaac 
ccttgagca tgtagtcac tacattatc tcatatcat 
ccgcaacat ttgtgtgtt taagtgata gtgagctga 
catacagtc ttacagtag cgataatct tccaaaaag 
taaaaaaat gttctaatt tgtgcaaat aaggttttg 
ttccatcat gcggcagat attatggtt gatcacgtt 
ttgcacaat tttgaaatt tgccttttg cgaaattat 
ttgcaacga tcccaaagg aagttgtgg atgtttagg 
tcgtcgcgt attatgttc tacggcaaa aaccgacga 
tatcatcat tgatatcag caaaggaat aattgacat 
ggagtgtcc cagagaatt gaaacaact ccactttga 
aaagcctcc aaacgtgac tattgacag cgacatttc 
aaaatttac aatgcggta ttgtccact accaccgaa 
caacttcag agaaatttt cgcctggtt ttcgctaca 
aaatgtgga aacattcga attttacgc ctcggagct 
gaacgcctg caccttaac aaacaaata ttttcaatc 
attggaaaa tctgaaaca gttaattct ctctgataa 
cgcaattga tgatttgga cgaaaactt tttcacaaa 
catgccaca agtgaaact tctagacct caaaaataa 
tcgcatcaa attgacaaa atcgacttt caaaaattt 
acagttttt acaacactt ggatttgtc gtccaataa 
cataaaatt tgtacctca cggagcctt tcaagaact 
cgaaacgct gacaacttt gaatttgtt cgacaacca 
actgaccaa aatcgacga ttttacatt tgccggact 
ttcaaacct gcaatcgct cgaattaag cgccaacaa 
aatacaaac aataagtga aaacgcatt cgccactct 
taaaaatct gacacggat taatctttc aaacaactt 
ccttaaaac actgcctgg ggggttatt ccaaggcaa 
cagaaacct aaaaacgct cagacttaa acacaatat 
tgggttaca actcccggg tttagtttt cgcaaattt 
attcctaac cgaagtgga tttgacgaa gtgtcgttt 
aggcgagat ccccgaaaa tgtttttga gaacacgac 
aacgttgaa agttgtcga gttgggagg aaatgattt 
ggaagactt gccagagaa cgtgttcaa aggcttgac 
gaatttggg caaaatttc tttgcaaca caacaagat 
taagtcaat ttcacactt gttcaaagg acttgagcg 
aattacttt gctgcagct acaaaaaaa ctcaattga 
gaaaattga gagcgaagc tttcgccga tttgataaa 
tctggaaaa aattaactt gcgggggaa tagaattaa 
acaaattaa cccgcttgt tttttcaag gaatcataa 
actaaaaac cgtcgtttt ggctgataa tgagatagt 
ggataagtt tgaggaggc aataacttc aactcaaac 
aaataactt gattcgctg gaatttgcg ccacaaccg 
gatccagca aataaagct cgcgaactg gaaaaacgt 
atcaataac actcgatta caatccact tatttgtaa 
ctgtctcaa ttacaattt actgaacca tatcagaaa 
ccagacaag aagtaataa cttgacaat aactgcaaa 
aaatgttaa atgtgaggg ctctcaagt gtttgagaa 
tcttgaact gtcgagtct cacatgttc actggacga 
aatttccaa aaattattt gtgtcctaa aaactgctc 
gtgcatttg gagaccttt cgacgcttc tgtcgtaat 
caactgttc aaataaatc gctcgttaa gtacccaca 
agtggacct aagtcagtt aacttacgt ttacaacga 
cattgaatt acaccttga aaataataa attggaatc 
agggcctga gcgccgttt tggttttct caatattac 
aaaattgtt tctttcaaa caacacaat caggtcaat 
tgattgggt gccaccaag gcttaagtc attacattt 
ggacaaaaa ccgcatcag caaaatccc tcccaaagt 
cattgaggt tttaaaaca gacaacttt ggtaaattt 
gactttaaa cgacaatcc ttggatgtg cgattgtgg 
ggctgaaat tttggccca atttttgcg tacatttcc 
gaacttttc cactaacat ttattgcgc taacacaac 
ccttcgcct aattgatat gaaaaagga ggacttttg 
caaaagcca gactgtcct ttatttaac aacactaac 
cctctcact ccttctcat ttcagccct tttggccat 
tttctacta ccggaacca ggaaactat caacttctg 
gcttctttc ccacaattt gtgttggtt tagtgaaga 
aaatcgcga taaagacaa gatctacga cgtttttat 
cagttattc acataaaga cgaacattt tgtcgaggc 
tctcttatc gttccttga aaaaggtcc caattcttt 
taaaacttg cgtacatta tcgtgattg gacgcctgg 
agagcctat cgtgagcca aatcatcaa ttccgtggt 
gaattcgcg ccgaacttt ggtggtgct ttcaaggaa 
tttcctcga aagtgtttg ggggaaaat ggagtttaa 
gacggcgca caggcaagc tttgagcga tggaaggaa 
tcgggttgt ggtagtttg gtatggaga tgttgaagt 
tgagaagtt ggatgagga gttgaaggt gtatttgcg 
gactaatac ttttgttaa gtgggggga taagttttt 
ttgggaaaa gctgaagat agcgttgcg acggactgt 
gaaatgggg gtccaaaat gggggtaca agaggagga 
agatatgtt aatagtgta gtcagtctc tttgaagac 
tttttgtat taatacagg gccagggcc gagccaaga 
ccgcgtgta ggtcccaaa tattgcgcc ccttactcg 
tataaaaat attaaaaaa aataataat aagttaaat 
aaatgaaat aaaaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY