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Gene information
General information
TC004452 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 2717157..2720408
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004452
    iBeetle screen iB_03761
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004452 RA
      MRQILWLIT FCTILKFAF CEIICNDPK GSCGCLGRR 
      VLCSTAKTD DIIIDISKG IIDMECPRE LKQLHFEKP 
      PNVTIDSDI SKFTMRYCP LPPNNFREI FAWFSLQNV 
      ETFEFYASE LNACTLTNK YFQSLENLK QLILSDNAI 
      DDLDENFFT NMPQVKLLD LKNNRIKLT KSTFKNLQF 
      LQHLDLSSN NIKFVPHGA FQELETLTT LNLFDNQLT 
      KIDDFTFAG LSNLQSLEL SANKIQTIS ENAFATLKN 
      LTRINLSNN FLKTLPGGL FQGNRNLKT LRLKHNIGL 
      QLPGLVFAN LFLTEVDLT KCRLGEIPE NVFENTTTL 
      KVVELGGND LEDLPENVF KGLTNLGKI SLQHNKIKS 
      ISHLFKGLE RITLLQLQK NSIEKIESE AFADLINLE 
      KINLRGNRI KQINPLVFS RNHKLKTVV LADNEIVDK 
      FEEAITSTQ TNNLIRWNL RHNRIQQIK LANWKNVSI 
      TLDYNPLIC NCLNYNLLN HIRNQTRSN NLTITAKNV 
      KCEGSQVFE NLELSSLTC SLDEISKNY LCPKNCSCI 
      WRPFDASVV INCSNKSLV KYPQVDLSQ LTYVYNDIE 
      LHLENNKLE SGPERRFGF LNITKLFLS NNTIRSIDW 
      VPPRLKSLH LDKNRISKI PPKVIEVLK QTTLVNLTL 
      NDNPWMCDC GAEILAQFL RTFPNFSTN IYCANTTLR 
      LIDMKKEDF CKSQTVLYL TTLTLSLLL ISALLAIFY 
      YRNQETINF WLLSHNLCW FSEENRDKD KIYDVFISY 
      SHKDEHFVE ALLSFLEKG PNSFKTCVH YRDWTPGEP 
      IVSQIINSV VNSRRTLVV LSRNFLESV WGKMEFKTA 
      HRQALSDGR NRVVVVWYG DVEVEKLDE ELKVYLRTN 
      TFVKWGDKF FWEKLKIAL RRTVKWGSK MGVQEEEDM 
      LIV
    CDS sequence
    • >TC004452 RA
      atgcggcag atattatgg ttgatcacg ttttgcaca 
      attttgaaa tttgccttt tgcgaaatt atttgcaac 
      gatcccaaa ggaagttgt ggatgttta ggtcgtcgc 
      gtattatgt tctacggca aaaaccgac gatatcatc 
      attgatatc agcaaagga ataattgac atggagtgt 
      cccagagaa ttgaaacaa ctccacttt gaaaagcct 
      ccaaacgtg actattgac agcgacatt tcaaaattt 
      acaatgcgg tattgtcca ctaccaccg aacaacttc 
      agagaaatt ttcgcctgg ttttcgcta caaaatgtg 
      gaaacattc gaattttac gcctcggag ctgaacgcc 
      tgcacctta acaaacaaa tattttcaa tcattggaa 
      aatctgaaa cagttaatt ctctctgat aacgcaatt 
      gatgatttg gacgaaaac tttttcaca aacatgcca 
      caagtgaaa cttctagac ctcaaaaat aatcgcatc 
      aaattgaca aaatcgact ttcaaaaat ttacagttt 
      ttacaacac ttggatttg tcgtccaat aacataaaa 
      tttgtacct cacggagcc tttcaagaa ctcgaaacg 
      ctgacaact ttgaatttg ttcgacaac caactgacc 
      aaaatcgac gattttaca tttgccgga ctttcaaac 
      ctgcaatcg ctcgaatta agcgccaac aaaatacaa 
      acaataagt gaaaacgca ttcgccact cttaaaaat 
      ctgacacgg attaatctt tcaaacaac ttccttaaa 
      acactgcct ggggggtta ttccaaggc aacagaaac 
      ctaaaaacg ctcagactt aaacacaat attgggtta 
      caactcccg ggtttagtt ttcgcaaat ttattccta 
      accgaagtg gatttgacg aagtgtcgt ttaggcgag 
      atccccgaa aatgttttt gagaacacg acaacgttg 
      aaagttgtc gagttggga ggaaatgat ttggaagac 
      ttgccagag aacgtgttc aaaggcttg acgaatttg 
      ggcaaaatt tctttgcaa cacaacaag attaagtca 
      atttcacac ttgttcaaa ggacttgag cgaattact 
      ttgctgcag ctacaaaaa aactcaatt gagaaaatt 
      gagagcgaa gctttcgcc gatttgata aatctggaa 
      aaaattaac ttgcggggg aatagaatt aaacaaatt 
      aacccgctt gttttttca aggaatcat aaactaaaa 
      accgtcgtt ttggctgat aatgagata gtggataag 
      tttgaggag gcaataact tcaactcaa acaaataac 
      ttgattcgc tggaatttg cgccacaac cggatccag 
      caaataaag ctcgcgaac tggaaaaac gtatcaata 
      acactcgat tacaatcca cttatttgt aactgtctc 
      aattacaat ttactgaac catatcaga aaccagaca 
      agaagtaat aacttgaca ataactgca aaaaatgtt 
      aaatgtgag ggctctcaa gtgtttgag aatcttgaa 
      ctgtcgagt ctcacatgt tcactggac gaaatttcc 
      aaaaattat ttgtgtcct aaaaactgc tcgtgcatt 
      tggagacct ttcgacgct tctgtcgta atcaactgt 
      tcaaataaa tcgctcgtt aagtaccca caagtggac 
      ctaagtcag ttaacttac gtttacaac gacattgaa 
      ttacacctt gaaaataat aaattggaa tcagggcct 
      gagcgccgt tttggtttt ctcaatatt acaaaattg 
      tttctttca aacaacaca atcaggtca attgattgg 
      gtgccacca aggcttaag tcattacat ttggacaaa 
      aaccgcatc agcaaaatc cctcccaaa gtcattgag 
      gttttaaaa cagacaact ttggtaaat ttgacttta 
      aacgacaat ccttggatg tgcgattgt ggggctgaa 
      attttggcc caatttttg cgtacattt ccgaacttt 
      tccactaac atttattgc gctaacaca acccttcgc 
      ctaattgat atgaaaaag gaggacttt tgcaaaagc 
      cagactgtc ctttattta acaacacta accctctca 
      ctccttctc atttcagcc cttttggcc attttctac 
      taccggaac caggaaact atcaacttc tggcttctt 
      tcccacaat ttgtgttgg tttagtgaa gaaaatcgc 
      gataaagac aagatctac gacgttttt atcagttat 
      tcacataaa gacgaacat tttgtcgag gctctctta 
      tcgttcctt gaaaaaggt cccaattct tttaaaact 
      tgcgtacat tatcgtgat tggacgcct ggagagcct 
      atcgtgagc caaatcatc aattccgtg gtgaattcg 
      cgccgaact ttggtggtg ctttcaagg aatttcctc 
      gaaagtgtt tgggggaaa atggagttt aagacggcg 
      cacaggcaa gctttgagc gatggaagg aatcgggtt 
      gtggtagtt tggtatgga gatgttgaa gttgagaag 
      ttggatgag gagttgaag gtgtatttg cggactaat 
      acttttgtt aagtggggg gataagttt ttttgggaa 
      aagctgaag atagcgttg cgacggact gtgaaatgg 
      gggtccaaa atgggggta caagaggag gaagatatg 
      ttaatagtg tag
    mRNA sequence
    • >TC004452 RA
      tttttgata gggtatcag ttttcgcga aaaaggaac 
      ccttgagca tgtagtcac tacattatc tcatatcat 
      ccgcaacat ttgtgtgtt taagtgata gtgagctga 
      catacagtc ttacagtag cgataatct tccaaaaag 
      taaaaaaat gttctaatt tgtgcaaat aaggttttg 
      ttccatcat gcggcagat attatggtt gatcacgtt 
      ttgcacaat tttgaaatt tgccttttg cgaaattat 
      ttgcaacga tcccaaagg aagttgtgg atgtttagg 
      tcgtcgcgt attatgttc tacggcaaa aaccgacga 
      tatcatcat tgatatcag caaaggaat aattgacat 
      ggagtgtcc cagagaatt gaaacaact ccactttga 
      aaagcctcc aaacgtgac tattgacag cgacatttc 
      aaaatttac aatgcggta ttgtccact accaccgaa 
      caacttcag agaaatttt cgcctggtt ttcgctaca 
      aaatgtgga aacattcga attttacgc ctcggagct 
      gaacgcctg caccttaac aaacaaata ttttcaatc 
      attggaaaa tctgaaaca gttaattct ctctgataa 
      cgcaattga tgatttgga cgaaaactt tttcacaaa 
      catgccaca agtgaaact tctagacct caaaaataa 
      tcgcatcaa attgacaaa atcgacttt caaaaattt 
      acagttttt acaacactt ggatttgtc gtccaataa 
      cataaaatt tgtacctca cggagcctt tcaagaact 
      cgaaacgct gacaacttt gaatttgtt cgacaacca 
      actgaccaa aatcgacga ttttacatt tgccggact 
      ttcaaacct gcaatcgct cgaattaag cgccaacaa 
      aatacaaac aataagtga aaacgcatt cgccactct 
      taaaaatct gacacggat taatctttc aaacaactt 
      ccttaaaac actgcctgg ggggttatt ccaaggcaa 
      cagaaacct aaaaacgct cagacttaa acacaatat 
      tgggttaca actcccggg tttagtttt cgcaaattt 
      attcctaac cgaagtgga tttgacgaa gtgtcgttt 
      aggcgagat ccccgaaaa tgtttttga gaacacgac 
      aacgttgaa agttgtcga gttgggagg aaatgattt 
      ggaagactt gccagagaa cgtgttcaa aggcttgac 
      gaatttggg caaaatttc tttgcaaca caacaagat 
      taagtcaat ttcacactt gttcaaagg acttgagcg 
      aattacttt gctgcagct acaaaaaaa ctcaattga 
      gaaaattga gagcgaagc tttcgccga tttgataaa 
      tctggaaaa aattaactt gcgggggaa tagaattaa 
      acaaattaa cccgcttgt tttttcaag gaatcataa 
      actaaaaac cgtcgtttt ggctgataa tgagatagt 
      ggataagtt tgaggaggc aataacttc aactcaaac 
      aaataactt gattcgctg gaatttgcg ccacaaccg 
      gatccagca aataaagct cgcgaactg gaaaaacgt 
      atcaataac actcgatta caatccact tatttgtaa 
      ctgtctcaa ttacaattt actgaacca tatcagaaa 
      ccagacaag aagtaataa cttgacaat aactgcaaa 
      aaatgttaa atgtgaggg ctctcaagt gtttgagaa 
      tcttgaact gtcgagtct cacatgttc actggacga 
      aatttccaa aaattattt gtgtcctaa aaactgctc 
      gtgcatttg gagaccttt cgacgcttc tgtcgtaat 
      caactgttc aaataaatc gctcgttaa gtacccaca 
      agtggacct aagtcagtt aacttacgt ttacaacga 
      cattgaatt acaccttga aaataataa attggaatc 
      agggcctga gcgccgttt tggttttct caatattac 
      aaaattgtt tctttcaaa caacacaat caggtcaat 
      tgattgggt gccaccaag gcttaagtc attacattt 
      ggacaaaaa ccgcatcag caaaatccc tcccaaagt 
      cattgaggt tttaaaaca gacaacttt ggtaaattt 
      gactttaaa cgacaatcc ttggatgtg cgattgtgg 
      ggctgaaat tttggccca atttttgcg tacatttcc 
      gaacttttc cactaacat ttattgcgc taacacaac 
      ccttcgcct aattgatat gaaaaagga ggacttttg 
      caaaagcca gactgtcct ttatttaac aacactaac 
      cctctcact ccttctcat ttcagccct tttggccat 
      tttctacta ccggaacca ggaaactat caacttctg 
      gcttctttc ccacaattt gtgttggtt tagtgaaga 
      aaatcgcga taaagacaa gatctacga cgtttttat 
      cagttattc acataaaga cgaacattt tgtcgaggc 
      tctcttatc gttccttga aaaaggtcc caattcttt 
      taaaacttg cgtacatta tcgtgattg gacgcctgg 
      agagcctat cgtgagcca aatcatcaa ttccgtggt 
      gaattcgcg ccgaacttt ggtggtgct ttcaaggaa 
      tttcctcga aagtgtttg ggggaaaat ggagtttaa 
      gacggcgca caggcaagc tttgagcga tggaaggaa 
      tcgggttgt ggtagtttg gtatggaga tgttgaagt 
      tgagaagtt ggatgagga gttgaaggt gtatttgcg 
      gactaatac ttttgttaa gtgggggga taagttttt 
      ttgggaaaa gctgaagat agcgttgcg acggactgt 
      gaaatgggg gtccaaaat gggggtaca agaggagga 
      agatatgtt aatagtgta gtcagtctc tttgaagac 
      tttttgtat taatacagg gccagggcc gagccaaga 
      ccgcgtgta ggtcccaaa tattgcgcc ccttactcg 
      tataaaaat attaaaaaa aataataat aagttaaat 
      aaatgaaat aaaaaaaa