| General information | |||
| TC004452 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 4806890..4810141 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004452 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03761 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC004452 RA
MRQILWLIT FCTILKFAF CEIICNDPK GSCGCLGRR VLCSTAKTD DIIIDISKG IIDMECPRE LKQLHFEKP PNVTIDSDI SKFTMRYCP LPPNNFREI FAWFSLQNV ETFEFYASE LNACTLTNK YFQSLENLK QLILSDNAI DDLDENFFT NMPQVKLLD LKNNRIKLT KSTFKNLQF LQHLDLSSN NIKFVPHGA FQELETLTT LNLFDNQLT KIDDFTFAG LSNLQSLEL SANKIQTIS ENAFATLKN LTRINLSNN FLKTLPGGL FQGNRNLKT LRLKHNIGL QLPGLVFAN LFLTEVDLT KCRLGEIPE NVFENTTTL KVVELGGND LEDLPENVF KGLTNLGKI SLQHNKIKS ISHLFKGLE RITLLQLQK NSIEKIESE AFADLINLE KINLRGNRI KQINPLVFS RNHKLKTVV LADNEIVDK FEEAITSTQ TNNLIRWNL RHNRIQQIK LANWKNVSI TLDYNPLIC NCLNYNLLN HIRNQTRSN NLTITAKNV KCEGSQVFE NLELSSLTC SLDEISKNY LCPKNCSCI WRPFDASVV INCSNKSLV KYPQVDLSQ LTYVYNDIE LHLENNKLE SGPERRFGF LNITKLFLS NNTIRSIDW VPPRLKSLH LDKNRISKI PPKVIEVLK QTTLVNLTL NDNPWMCDC GAEILAQFL RTFPNFSTN IYCANTTLR LIDMKKEDF CKSQTVLYL TTLTLSLLL ISALLAIFY YRNQETINF WLLSHNLCW FSEENRDKD KIYDVFISY SHKDEHFVE ALLSFLEKG PNSFKTCVH YRDWTPGEP IVSQIINSV VNSRRTLVV LSRNFLESV WGKMEFKTA HRQALSDGR NRVVVVWYG DVEVEKLDE ELKVYLRTN TFVKWGDKF FWEKLKIAL RRTVKWGSK MGVQEEEDM LIV
- >TC004452 RA
atgcggcag atattatgg ttgatcacg ttttgcaca attttgaaa tttgccttt tgcgaaatt atttgcaac gatcccaaa ggaagttgt ggatgttta ggtcgtcgc gtattatgt tctacggca aaaaccgac gatatcatc attgatatc agcaaagga ataattgac atggagtgt cccagagaa ttgaaacaa ctccacttt gaaaagcct ccaaacgtg actattgac agcgacatt tcaaaattt acaatgcgg tattgtcca ctaccaccg aacaacttc agagaaatt ttcgcctgg ttttcgcta caaaatgtg gaaacattc gaattttac gcctcggag ctgaacgcc tgcacctta acaaacaaa tattttcaa tcattggaa aatctgaaa cagttaatt ctctctgat aacgcaatt gatgatttg gacgaaaac tttttcaca aacatgcca caagtgaaa cttctagac ctcaaaaat aatcgcatc aaattgaca aaatcgact ttcaaaaat ttacagttt ttacaacac ttggatttg tcgtccaat aacataaaa tttgtacct cacggagcc tttcaagaa ctcgaaacg ctgacaact ttgaatttg ttcgacaac caactgacc aaaatcgac gattttaca tttgccgga ctttcaaac ctgcaatcg ctcgaatta agcgccaac aaaatacaa acaataagt gaaaacgca ttcgccact cttaaaaat ctgacacgg attaatctt tcaaacaac ttccttaaa acactgcct ggggggtta ttccaaggc aacagaaac ctaaaaacg ctcagactt aaacacaat attgggtta caactcccg ggtttagtt ttcgcaaat ttattccta accgaagtg gatttgacg aagtgtcgt ttaggcgag atccccgaa aatgttttt gagaacacg acaacgttg aaagttgtc gagttggga ggaaatgat ttggaagac ttgccagag aacgtgttc aaaggcttg acgaatttg ggcaaaatt tctttgcaa cacaacaag attaagtca atttcacac ttgttcaaa ggacttgag cgaattact ttgctgcag ctacaaaaa aactcaatt gagaaaatt gagagcgaa gctttcgcc gatttgata aatctggaa aaaattaac ttgcggggg aatagaatt aaacaaatt aacccgctt gttttttca aggaatcat aaactaaaa accgtcgtt ttggctgat aatgagata gtggataag tttgaggag gcaataact tcaactcaa acaaataac ttgattcgc tggaatttg cgccacaac cggatccag caaataaag ctcgcgaac tggaaaaac gtatcaata acactcgat tacaatcca cttatttgt aactgtctc aattacaat ttactgaac catatcaga aaccagaca agaagtaat aacttgaca ataactgca aaaaatgtt aaatgtgag ggctctcaa gtgtttgag aatcttgaa ctgtcgagt ctcacatgt tcactggac gaaatttcc aaaaattat ttgtgtcct aaaaactgc tcgtgcatt tggagacct ttcgacgct tctgtcgta atcaactgt tcaaataaa tcgctcgtt aagtaccca caagtggac ctaagtcag ttaacttac gtttacaac gacattgaa ttacacctt gaaaataat aaattggaa tcagggcct gagcgccgt tttggtttt ctcaatatt acaaaattg tttctttca aacaacaca atcaggtca attgattgg gtgccacca aggcttaag tcattacat ttggacaaa aaccgcatc agcaaaatc cctcccaaa gtcattgag gttttaaaa cagacaact ttggtaaat ttgacttta aacgacaat ccttggatg tgcgattgt ggggctgaa attttggcc caatttttg cgtacattt ccgaacttt tccactaac atttattgc gctaacaca acccttcgc ctaattgat atgaaaaag gaggacttt tgcaaaagc cagactgtc ctttattta acaacacta accctctca ctccttctc atttcagcc cttttggcc attttctac taccggaac caggaaact atcaacttc tggcttctt tcccacaat ttgtgttgg tttagtgaa gaaaatcgc gataaagac aagatctac gacgttttt atcagttat tcacataaa gacgaacat tttgtcgag gctctctta tcgttcctt gaaaaaggt cccaattct tttaaaact tgcgtacat tatcgtgat tggacgcct ggagagcct atcgtgagc caaatcatc aattccgtg gtgaattcg cgccgaact ttggtggtg ctttcaagg aatttcctc gaaagtgtt tgggggaaa atggagttt aagacggcg cacaggcaa gctttgagc gatggaagg aatcgggtt gtggtagtt tggtatgga gatgttgaa gttgagaag ttggatgag gagttgaag gtgtatttg cggactaat acttttgtt aagtggggg gataagttt ttttgggaa aagctgaag atagcgttg cgacggact gtgaaatgg gggtccaaa atgggggta caagaggag gaagatatg ttaatagtg tag
- >TC004452 RA
tttttgata gggtatcag ttttcgcga aaaaggaac ccttgagca tgtagtcac tacattatc tcatatcat ccgcaacat ttgtgtgtt taagtgata gtgagctga catacagtc ttacagtag cgataatct tccaaaaag taaaaaaat gttctaatt tgtgcaaat aaggttttg ttccatcat gcggcagat attatggtt gatcacgtt ttgcacaat tttgaaatt tgccttttg cgaaattat ttgcaacga tcccaaagg aagttgtgg atgtttagg tcgtcgcgt attatgttc tacggcaaa aaccgacga tatcatcat tgatatcag caaaggaat aattgacat ggagtgtcc cagagaatt gaaacaact ccactttga aaagcctcc aaacgtgac tattgacag cgacatttc aaaatttac aatgcggta ttgtccact accaccgaa caacttcag agaaatttt cgcctggtt ttcgctaca aaatgtgga aacattcga attttacgc ctcggagct gaacgcctg caccttaac aaacaaata ttttcaatc attggaaaa tctgaaaca gttaattct ctctgataa cgcaattga tgatttgga cgaaaactt tttcacaaa catgccaca agtgaaact tctagacct caaaaataa tcgcatcaa attgacaaa atcgacttt caaaaattt acagttttt acaacactt ggatttgtc gtccaataa cataaaatt tgtacctca cggagcctt tcaagaact cgaaacgct gacaacttt gaatttgtt cgacaacca actgaccaa aatcgacga ttttacatt tgccggact ttcaaacct gcaatcgct cgaattaag cgccaacaa aatacaaac aataagtga aaacgcatt cgccactct taaaaatct gacacggat taatctttc aaacaactt ccttaaaac actgcctgg ggggttatt ccaaggcaa cagaaacct aaaaacgct cagacttaa acacaatat tgggttaca actcccggg tttagtttt cgcaaattt attcctaac cgaagtgga tttgacgaa gtgtcgttt aggcgagat ccccgaaaa tgtttttga gaacacgac aacgttgaa agttgtcga gttgggagg aaatgattt ggaagactt gccagagaa cgtgttcaa aggcttgac gaatttggg caaaatttc tttgcaaca caacaagat taagtcaat ttcacactt gttcaaagg acttgagcg aattacttt gctgcagct acaaaaaaa ctcaattga gaaaattga gagcgaagc tttcgccga tttgataaa tctggaaaa aattaactt gcgggggaa tagaattaa acaaattaa cccgcttgt tttttcaag gaatcataa actaaaaac cgtcgtttt ggctgataa tgagatagt ggataagtt tgaggaggc aataacttc aactcaaac aaataactt gattcgctg gaatttgcg ccacaaccg gatccagca aataaagct cgcgaactg gaaaaacgt atcaataac actcgatta caatccact tatttgtaa ctgtctcaa ttacaattt actgaacca tatcagaaa ccagacaag aagtaataa cttgacaat aactgcaaa aaatgttaa atgtgaggg ctctcaagt gtttgagaa tcttgaact gtcgagtct cacatgttc actggacga aatttccaa aaattattt gtgtcctaa aaactgctc gtgcatttg gagaccttt cgacgcttc tgtcgtaat caactgttc aaataaatc gctcgttaa gtacccaca agtggacct aagtcagtt aacttacgt ttacaacga cattgaatt acaccttga aaataataa attggaatc agggcctga gcgccgttt tggttttct caatattac aaaattgtt tctttcaaa caacacaat caggtcaat tgattgggt gccaccaag gcttaagtc attacattt ggacaaaaa ccgcatcag caaaatccc tcccaaagt cattgaggt tttaaaaca gacaacttt ggtaaattt gactttaaa cgacaatcc ttggatgtg cgattgtgg ggctgaaat tttggccca atttttgcg tacatttcc gaacttttc cactaacat ttattgcgc taacacaac ccttcgcct aattgatat gaaaaagga ggacttttg caaaagcca gactgtcct ttatttaac aacactaac cctctcact ccttctcat ttcagccct tttggccat tttctacta ccggaacca ggaaactat caacttctg gcttctttc ccacaattt gtgttggtt tagtgaaga aaatcgcga taaagacaa gatctacga cgtttttat cagttattc acataaaga cgaacattt tgtcgaggc tctcttatc gttccttga aaaaggtcc caattcttt taaaacttg cgtacatta tcgtgattg gacgcctgg agagcctat cgtgagcca aatcatcaa ttccgtggt gaattcgcg ccgaacttt ggtggtgct ttcaaggaa tttcctcga aagtgtttg ggggaaaat ggagtttaa gacggcgca caggcaagc tttgagcga tggaaggaa tcgggttgt ggtagtttg gtatggaga tgttgaagt tgagaagtt ggatgagga gttgaaggt gtatttgcg gactaatac ttttgttaa gtgggggga taagttttt ttgggaaaa gctgaagat agcgttgcg acggactgt gaaatgggg gtccaaaat gggggtaca agaggagga agatatgtt aatagtgta gtcagtctc tttgaagac tttttgtat taatacagg gccagggcc gagccaaga ccgcgtgta ggtcccaaa tattgcgcc ccttactcg tataaaaat attaaaaaa aataataat aagttaaat aaatgaaat aaaaaaaa
