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Gene information
General information
TC004451 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid6: 2734578..2744378
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004451
    iBeetle screen iB_03760
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004451 RA
      MTLALGMHV IIEKGSVVM ALYCPFWML NKTGLMLAY 
      RNSEDCVNV LHHPANFKG PILFSFNAK NFFGKKKAA 
      VRVEQGDWS DKFSLDVAG SSGVVVCKY QNLLYQIGV 
      QNQLTYNGL TKQVTFTPY YVIINNASF AIEFQENDR 
      PADPWICVE PKSCSALWP KSELDDKLL KLRVKGTEE 
      VSAAFLYTE SHTSLLKLK NRYGGINVD IQISEGAIY 
      INLAPYDGN TAPALIINH TIDTMNFWE KESVQIRKL 
      PSKHSMLYT WENPSGPRI LVWEGANKK EHSNDLRKD 
      DMGEYTIRE GYHIYWVSF LDGMQRILL FTQDQYVAQ 
      NARAANLFE QFQQEITVS IHGVGLSLV NNITRQELM 
      YMAIASSGI IWEHCKMTG RRFKPFGNK ESLLLETAY 
      QRFLADIQE GNSPKVAID AKTEVDFKN NIMMKPNKR 
      RIRRTFQTG LWVQMRTSP SQLQVHAKI NRLQIDNQM 
      YDCIFPVVL APVPLPKSV AIDSGMKPF AELSIVQLL 
      MKNTQVKQF KYFKLLIQE FHVKVDLGF VNALVDMLE 
      SSEYSETEE QQLFLKDMS LVDEPLYSH VSTQSLQEQ 
      KSFYDLLHF SPLKIHVSF SMAAGTSAG QSSSTPNFL 
      NVLLQGLGV TLTDLQDVV FRLAYFERE YTFLTQKQL 
      MSEATSHYV GQSVKQLYV LVLGLDVLG NPYGLVRGI 
      TQGVEDLFY EPFQGAIQG PGEFAEGLV LGVRSLFGH 
      TVGGAAGAV SRITGAMGK GIAALTFDK DYQRKRRDQ 
      INKRPATVQ EGIARSGKG LVMGVVDGV TGVFTKPIS 
      GAKEEGVGG FFKGLGKGA VGLIARPTA GVVDFASGS 
      LDAVKRVTE VGEDANRLR PPRFLQGDG LVRPYNRYE 
      AQGHKLLTD LEKGKYATT DIYDSHYVI VENREILLL 
      TDKRLAFIS HNDIFGGWQ VEWSYTWQE IKNPPSVVP 
      KGVAIPTSD RRKKLGLLG NSDNGKIIL VGDPQKKEE 
      ICAKIESLR GHI
    CDS sequence
    • >TC004451 RA
      atgactttg gccctgggt atgcacgtg attatcgaa 
      aaaggttca gtcgtcatg gccctttat tgtcccttc 
      tggatgttg aacaaaacc ggcctcatg ctggcatat 
      cgaaattct gaagattgc gtcaatgtg ctgcaccac 
      ccggccaat ttcaaaggt ccgattttg ttttctttc 
      aacgcgaag aatttcttc gggaagaag aaagcagct 
      gttagggtc gagcaaggg gattggtcg gataaattc 
      tcgttagat gtcgcagga tcctctgga gtggttgtt 
      tgcaaatac cagaatttg ctatatcag attggtgta 
      caaaatcaa ctgacttac aatggtcta acgaaacaa 
      gtgactttc acaccgtat tatgttatt attaataat 
      gcgtctttc gctattgaa tttcaagaa aatgacagg 
      cctgctgat ccatggatt tgtgttgag ccaaaatcc 
      tgttcggca ctttggcct aaaagcgaa ttagatgac 
      aagttgctg aaattaagg gttaaaggt acagaagag 
      gtttcagca gcgtttctg tacactgag agtcatact 
      tcgctactg aagctgaaa aataggtac ggtggcatc 
      aacgtggac atccaaatt tccgaaggc gccatctac 
      ataaacctc gccccttac gacggcaat acagcccca 
      gctcttatc ataaaccac accatcgat acaatgaac 
      ttctgggag aaagaatca gtccaaatt cgaaaactc 
      ccctccaaa cacagcatg ttatacacg tgggagaac 
      ccctcaggt ccccgcatc ctagtatgg gaaggtgcg 
      aacaaaaag gagcacagt aacgatctc cgcaaagac 
      gacatgggc gaatacaca ataagagaa ggttaccac 
      atttattgg gtttcattc ctcgacgga atgcagcga 
      attctactc ttcacccaa gaccaatac gtggcgcaa 
      aacgcgcga gctgcgaat ttattcgaa cagttccag 
      caagaaatc accgtttcg atacacggt gtcggcttg 
      tcgcttgtc aataacata acgcgccaa gaactgatg 
      tacatggcg attgcaagt tcgggcata atctgggaa 
      cattgcaaa atgaccggt cggaggttc aaaccgttc 
      gggaataag gagagcttg ttgttggag acggcctat 
      cagcggttt ttggccgat attcaggaa ggcaattcg 
      ccgaaagtt gcaatcgat gctaagacc gaagtcgat 
      tttaaaaac aacattatg atgaaaccg aacaagaga 
      cgcataagg aggacgttc cagaccggg ctttgggtg 
      cagatgcgg accagtccg agccagttg caagtccat 
      gcgaaaatt aaccgactg caaatcgat aatcaaatg 
      tatgactgc attttcccc gtggttttg gcgccggtt 
      ccgttaccc aaaagtgtg gccattgac agcggcatg 
      aagccgttc gctgagctc agtatcgtt caacttttg 
      atgaaaaac acccaagtc aagcagttc aagtatttc 
      aaactcctg atacaggag tttcacgtt aaggtcgat 
      ctgggtttc gttaacgcc ctagtggat atgttagaa 
      tcgtccgaa tattccgaa acggaagaa caacaactg 
      ttcttgaaa gatatgagt ctcgttgac gaaccgctc 
      tattcgcac gtttcgacg caatcgttg caagaacag 
      aagagtttc tacgacttg cttcacttt tcgccgctc 
      aaaatccat gtcagtttt tcaatggca gcggggact 
      tcagctggg caaagcagt tcgacgccg aatttcctc 
      aacgtgtta ctgcaaggt ctgggagta acactaacc 
      gatttacag gacgttgtc ttcagactg gcgtacttc 
      gaacgcgag tacactttc ctaactcag aaacaacta 
      atgagcgaa gccacaagt cactacgtt ggccagtcg 
      gtcaaacag ctttatgtc ctagtgttg ggtttggat 
      gttttgggg aatccctac gggctggtt cgggggatt 
      acccaaggt gttgaagat ttgttctac gaaccgttc 
      caaggtgcg attcaaggt ccgggcgag tttgctgaa 
      gggttggtg ttaggtgtt cggagtttg tttgggcat 
      acggtgggt ggcgctgct ggtgctgtg tcacgaatc 
      actggagct atggggaaa gggattgca gccttgacg 
      tttgataag gattatcaa aggaaacgg cgggatcag 
      attaataaa agaccggcg actgtgcaa gaggggatt 
      gctcgaagt ggcaaggga ttggttatg ggtgtggtc 
      gatggtgtt actggtgtt ttcaccaaa ccgatcagt 
      ggtgctaag gaggaagga gttggaggc tttttcaaa 
      ggattgggg aagggagca gtggggctc attgcacgg 
      cctactgcc ggtgttgta gatttcgca agcgggtca 
      ctggatgcc gttaaaagg gtgactgag gtgggcgaa 
      gacgcaaac cggttacgc ccaccgcgg tttttacaa 
      ggcgacggt ttggttaga ccctacaac aggtacgaa 
      gctcaggga cacaaactg ttaaccgat ttagaaaaa 
      ggcaaatac gcaacgact gacatttac gatagtcat 
      tatgttatt gttgaaaat cgcgaaatt ttgttactg 
      acagataag cgattggcg tttatttcg cataatgat 
      attttcggc ggctggcaa gtcgagtgg tcttataca 
      tggcaagaa atcaagaat ccgccaagt gtggtgcct 
      aaaggagtt gctattccc acttctgat aggaggaag 
      aagttgggt ttgttgggg aatagtgat aacgggaaa 
      attatccta gtcggagac ccacagaaa aaggaggaa 
      atatgcgcg aagattgag tcgttgaga ggtcatatt 
      taa
    mRNA sequence
    • >TC004451 RA
      ctataacga agtaaaacc gaggagttt ttatgattt 
      tctttgctt ttctgttta gttgccttc ttacttgga 
      aaaggagtg gtcatgcag acaagaact gcacaaaga 
      tgcgcccga attttccgt ttggaactt tgcaagtta 
      cgatagtgc cgtccaaat gactttggc cctgggtat 
      gcacgtgat tatcgaaaa aggttcagt cgtcatggc 
      cctttattg tcccttctg gatgttgaa caaaaccgg 
      cctcatgct ggcatatcg aaattctga agattgcgt 
      caatgtgct gcaccaccc ggccaattt caaaggtcc 
      gattttgtt ttctttcaa cgcgaagaa tttcttcgg 
      gaagaagaa agcagctgt tagggtcga gcaagggga 
      ttggtcgga taaattctc gttagatgt cgcaggatc 
      ctctggagt ggttgtttg caaatacca gaatttgct 
      atatcagat tggtgtaca aaatcaact gacttacaa 
      tggtctaac gaaacaagt gactttcac accgtatta 
      tgttattat taataatgc gtctttcgc tattgaatt 
      tcaagaaaa tgacaggcc tgctgatcc atggatttg 
      tgttgagcc aaaatcctg ttcggcact ttggcctaa 
      aagcgaatt agatgacaa gttgctgaa attaagggt 
      taaaggtac agaagaggt ttcagcagc gtttctgta 
      cactgagag tcatacttc gctactgaa gctgaaaaa 
      taggtacgg tggcatcaa cgtggacat ccaaatttc 
      cgaaggcgc catctacat aaacctcgc cccttacga 
      cggcaatac agccccagc tcttatcat aaaccacac 
      catcgatac aatgaactt ctgggagaa agaatcagt 
      ccaaattcg aaaactccc ctccaaaca cagcatgtt 
      atacacgtg ggagaaccc ctcaggtcc ccgcatcct 
      agtatggga aggtgcgaa caaaaagga gcacagtaa 
      cgatctccg caaagacga catgggcga atacacaat 
      aagagaagg ttaccacat ttattgggt ttcattcct 
      cgacggaat gcagcgaat tctactctt cacccaaga 
      ccaatacgt ggcgcaaaa cgcgcgagc tgcgaattt 
      attcgaaca gttccagca agaaatcac cgtttcgat 
      acacggtgt cggcttgtc gcttgtcaa taacataac 
      gcgccaaga actgatgta catggcgat tgcaagttc 
      gggcataat ctgggaaca ttgcaaaat gaccggtcg 
      gaggttcaa accgttcgg gaataagga gagcttgtt 
      gttggagac ggcctatca gcggttttt ggccgatat 
      tcaggaagg caattcgcc gaaagttgc aatcgatgc 
      taagaccga agtcgattt taaaaacaa cattatgat 
      gaaaccgaa caagagacg cataaggag gacgttcca 
      gaccgggct ttgggtgca gatgcggac cagtccgag 
      ccagttgca agtccatgc gaaaattaa ccgactgca 
      aatcgataa tcaaatgta tgactgcat tttccccgt 
      ggttttggc gccggttcc gttacccaa aagtgtggc 
      cattgacag cggcatgaa gccgttcgc tgagctcag 
      tatcgttca acttttgat gaaaaacac ccaagtcaa 
      gcagttcaa gtatttcaa actcctgat acaggagtt 
      tcacgttaa ggtcgatct gggtttcgt taacgccct 
      agtggatat gttagaatc gtccgaata ttccgaaac 
      ggaagaaca acaactgtt cttgaaaga tatgagtct 
      cgttgacga accgctcta ttcgcacgt ttcgacgca 
      atcgttgca agaacagaa gagtttcta cgacttgct 
      tcacttttc gccgctcaa aatccatgt cagtttttc 
      aatggcagc ggggacttc agctgggca aagcagttc 
      gacgccgaa tttcctcaa cgtgttact gcaaggtct 
      gggagtaac actaaccga tttacagga cgttgtctt 
      cagactggc gtacttcga acgcgagta cactttcct 
      aactcagaa acaactaat gagcgaagc cacaagtca 
      ctacgttgg ccagtcggt caaacagct ttatgtcct 
      agtgttggg tttggatgt tttggggaa tccctacgg 
      gctggttcg ggggattac ccaaggtgt tgaagattt 
      gttctacga accgttcca aggtgcgat tcaaggtcc 
      gggcgagtt tgctgaagg gttggtgtt aggtgttcg 
      gagtttgtt tgggcatac ggtgggtgg cgctgctgg 
      tgctgtgtc acgaatcac tggagctat ggggaaagg 
      gattgcagc cttgacgtt tgataagga ttatcaaag 
      gaaacggcg ggatcagat taataaaag accggcgac 
      tgtgcaaga ggggattgc tcgaagtgg caagggatt 
      ggttatggg tgtggtcga tggtgttac tggtgtttt 
      caccaaacc gatcagtgg tgctaagga ggaaggagt 
      tggaggctt tttcaaagg attggggaa gggagcagt 
      ggggctcat tgcacggcc tactgccgg tgttgtaga 
      tttcgcaag cgggtcact ggatgccgt taaaagggt 
      gactgaggt gggcgaaga cgcaaaccg gttacgccc 
      accgcggtt tttacaagg cgacggttt ggttagacc 
      ctacaacag gtacgaagc tcagggaca caaactgtt 
      aaccgattt agaaaaagg caaatacgc aacgactga 
      catttacga tagtcatta tgttattgt tgaaaatcg 
      cgaaatttt gttactgac agataagcg attggcgtt 
      tatttcgca taatgatat tttcggcgg ctggcaagt 
      cgagtggtc ttatacatg gcaagaaat caagaatcc 
      gccaagtgt ggtgcctaa aggagttgc tattcccac 
      ttctgatag gaggaagaa gttgggttt gttggggaa 
      tagtgataa cgggaaaat tatcctagt cggagaccc 
      acagaaaaa ggaggaaat atgcgcgaa gattgagtc 
      gttgagagg tcatattta aattgttag tttttattg 
      tttcactag tcatacgga ctgcctcat cctttgaag 
      tctttttta ctaatcttt atgcaacgt aattttatt 
      tgtgtatgc aacaacttg tgcttattt acgcttttc 
      ggcattggt gatgtactg ttgggaaaa taaaatgac 
      gtttttatc