Status
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Gene information
General information
TC004440 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 4894846..4915687
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004440
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03757

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004440 RA
      MVFESVLTQ VLNRFLGAY VENLDTNQL RVGIWGGDV 
      ELKDLVLKQ SALDDLDLP LQTIYGRIG KLVLKIPWK 
      NLYGAAFVI NIEDIYLLA APNQQVIYN EEKEEIKKS 
      EAKKREILK VELAKKQEA DKDKPKADN TFVEKLVTQ 
      IIKNIQLNI KNIHIRYED KVTSPTPFA LGITLSDLH 
      VVSTDENWK PAITKEDIT KIFKIVTLD GLAIYWNSN 
      TACYDGLPK VDMLHKMCT EIASKNNTP INYKYVLGP 
      INASARLRI NQRPELDKP AFTIPKIHL NLDMERLFV 
      GITKAQYRD IIALGDSMD RMMKGTPYR KFRPNVPSY 
      KGNYKTWWH FAYNCVLEG EVRRRRRNW DWNHICKHR 
      DLCREYGTL YEKKLQNKA TSDELSRIE ECEKVIDVF 
      NIVIIRQRV EFENERRVK TKGPEKGWF GWMWGSKTS 
      IDSDSLESD SGTELRKQF QNAMTKEEK ERLYKAIGY 
      QENALPSEF PEAYVDNSC TFILRTLEV ELSDDSGNV 
      KRVVFTELK GVKCRVDTR AAANALKLH VKIDEFQTF 
      GLQQDDFLP KIITSETGK DDSGLLQVL FETNPLDKK 
      CGQRLHVTS QPLKIVYDA QTVLKAIEI FKVPSSSAL 
      DQISAAADS GLSNMKEMT SSGLQHAID THVHLDLKV 
      DFRAPYIII PYGGKYTEN ENVLVVNLG NFQMHSLDR 
      PPRRDVRQM YAEGCDEKE ILTQLMAQS YDQFALELT 
      RVQILIAQS DEDWEVHVS EVKTSDMHI LEPINLKVN 
      LATCMITDD PKLPHMKIS GELPSIIVN VSDARLLLL 
      MALGNSIPL PQSDIPEPT PLAKVAKRE SSMMLRYLE 
      MQEKASKTQ TLLPSTAKK TDDQLVQFT TLEIHFAMS 
      ELLLNVKHQ SDIASPITN IACFSVKSL ELGLVQQTY 
      NTKITLDLG GVSLIQTHD SKEIKMISS PSFEEDEQY 
      LFQIKFTQV NKNSPEFHS TYKSCEASL ALEFAKLGI 
      ILHKEGLLS LIQFATSLQ TEIDDIWGS RPVERVAST 
      HTPSFKSQL STISEGLGS QLAPQEKKR KHKETKIVV 
      ETIKFKLSA NVQEVNVEF ATEHERISF WALKGVHSN 
      IIVKEPYTQ INANLNQIV VVDLNPKTI HNLILTVEG 
      TEALSTRIV LYNLDKMSE SDKSDISVD LTMGGLRII 
      FLNWFVTRM LDFLNEFQT AQAAIIEAS QAAAKTAKE 
      NMQNVYEKA TKLSLSINL KAPDIIIPE NSKSYDAIL 
      LDLGHISIS NQFLTMDIR NEQNFSAVI DEMKLNLTD 
      MKLSRVKLT TRDEIDKEL TLLVPLTFK LTIKRNLST 
      SWYKAVPDI DITGRIFSI TVLLSQLDY KTIMSVLNG 
      NLSEGQKKG APAKQPPKE VIEGTKVIS DDKALITIH 
      EKQSGHEEK DAKTNTFLR LTFTMDNLV MTLFTGGSK 
      ALSSDSPAH DERHALARF SLEGVSVKV RMLTDNSMI 
      ASFLLLNCL LDDMRVGRE GKLNRLVQR SQDFGAAPS 
      VSMVSISET SASKSMIDI VVQMKDKDI FTDVRVYSF 
      TLILSVDYL MKIADFLTI PQEEVKEAP KASKSNTST 
      KSAISSGMM EMEEKASQI TLNLKIEKP DIVLVEHMD 
      SIDTQAMIL NSEVVMKLR MSGEHQVIN GSIKDLQLY 
      TCNYNQLKR IDTKSNVLH PVTISLAGS TPENKGLHL 
      ELLVTDIRL SVSPATIEL LSRVTATAT GSSAERTSE 
      DEELQDHSD VWEHKSFED EDYWFFRND EAVEATEAL 
      SISSSIVPK SPNKTQEMC IISMPSIIL TIEAGVGSK 
      TFPMLVLES SLKGHVNNW SSQLSVEAG LTLQMNYYN 
      SRLALWEPL IEPVEVSHS NKPKWVPWE LKLEVSRNE 
      PEEVTTPTD LESLDSAPQ QAAMCIDVS SDHSIEIVV 
      TKTCLEVLN NLGKAFATA MKPGEVKML ETMAPFKIV 
      NELGCSVCV NLERGSFAL FGGEELAEV ILESGAEAQ 
      LQKKPMVLS SPSRLGLQL TTDALSVKN YFVNVKVID 
      KNCNIDLPV VRADKRFFP LNYRGDGND HWGLISDVT 
      VDEGVTIIT LRSILKVYN HFNIPIEVY YMTVRGNEL 
      ESITTIQPD SHVNIPLKA VYTPTNELF FSVGGYSVT 
      TAPYIWKDL QTNLTITKT LQCQPKEAE LGNDPFIIQ 
      AVGEMEQVY YENTSRHTM SSTCYNIHL RPAVVFKNC 
      LPIDIICCI ENTVSEIPV EPGKTLLLP NVEPGRSAL 
      VIRVSFKYG YR
    CDS sequence
    • >TC004440 RA
      atggtgttt gaaagcgta ttaactcag gtattaaac 
      cgcttcctt ggtgcctat gtagagaat ttagacaca 
      aatcaacta agagtggga atatgggga ggcgacgtc 
      gaattaaag gatttggtt ttgaagcag tccgcttta 
      gacgacttg gacctccct cttcaaact atctacggc 
      agaataggg aagctagtt ttgaaaata ccgtggaag 
      aatttatat ggggctgct ttcgttata aacattgaa 
      gatatctat cttcttgca gccccaaac caacaagtt 
      atctacaat gaagagaaa gaagaaatc aaaaagtcg 
      gaagccaag aaacgagag attcttaaa gttgaattg 
      gcgaaaaaa caagaagct gacaaggac aaacctaaa 
      gctgacaac acatttgtg gagaaatta gtgacacaa 
      atcatcaaa aatatccaa ttaaatatc aaaaatatt 
      catattagg tatgaggat aaggtcaca agtcctaca 
      ccttttgcg ttaggaatc acactgagt gatttacat 
      gttgtgagc actgatgag aactggaaa cctgccata 
      acgaaagaa gatatcaca aagatcttt aaaattgtt 
      acactggat ggtttagcg atttactgg aattcaaac 
      accgcttgc tacgatgga ctccctaaa gtcgacatg 
      ttacacaaa atgtgtaca gaaattgca tcaaaaaat 
      aatacaccc attaattac aagtatgtt ttgggacca 
      atcaacgcc tcggctcgc ctccgcatc aaccagcgc 
      cctgaactc gacaaaccc gctttcact attcccaaa 
      atccactta aatctcgac atggaacgc cttttcgtt 
      ggcataacc aaagcccaa tatcgcgat attatagct 
      cttggtgat tccatggac cgcatgatg aagggaacc 
      ccctaccgc aaattccgc cccaacgtg ccttcctac 
      aaaggcaac tacaaaacg tggtggcat ttcgcgtac 
      aattgcgtg ctggaaggc gaagtcagg cggaggaga 
      cgcaattgg gattggaac cacatttgc aaacatcgc 
      gatttgtgt cgggaatat gggactcta tacgagaaa 
      aaactgcaa aataaggcg accagtgat gagttgagt 
      cgtatcgaa gagtgcgag aaagtgatc gatgtcttc 
      aatattgtt attataaga cagagggtt gagtttgag 
      aatgagaga cgggtgaag actaaaggg ccagaaaag 
      ggttggttt gggtggatg tgggggagt aaaacttca 
      atcgattcg gatagtcta gagtctgat agtggcacc 
      gaactcagg aaacaattc caaaatgcg atgaccaaa 
      gaggagaaa gaacggctg tataaggct ataggttat 
      caggaaaat gcgttaccg tctgagttt cctgaggct 
      tacgtcgac aattcatgc acttttatt ctacgaacg 
      cttgaagtc gaattaagt gacgactca ggaaacgtc 
      aaaagggtt gtttttact gaacttaaa ggggtcaag 
      tgtcgcgtg gacaccaga gcggcggcc aatgcctta 
      aagcttcat gtcaaaatc gatgagttc caaacgttt 
      ggtctccaa caagacgat tttttgccg aaaataatc 
      acctcagag actggaaaa gacgacagt gggttgctt 
      caagtgctc ttcgagacc aatcctctc gataaaaaa 
      tgcggtcaa agactgcat gtcacctcc caaccgctt 
      aaaatcgtc tacgatgca caaactgtc ttgaaagcg 
      attgaaatt ttcaaagtt ccgtcttcg tcagctttg 
      gaccagatt tcggcagcg gccgattcc ggtctttct 
      aatatgaaa gaaatgact tcgagtggt ctccagcac 
      gcgattgac acccacgtt catctcgat ctcaaagta 
      gattttcgt gccccttac attataatc ccctatgga 
      gggaaatac accgaaaat gaaaatgtc ctagtggtc 
      aatttaggt aatttccaa atgcactca cttgatcgg 
      ccgccaagg agggatgtg aggcagatg tatgccgaa 
      gggtgcgac gagaaggag attttgaca cagctgatg 
      gctcaaagt tacgatcag tttgcgttg gaattgact 
      cgagtgcaa attctaatc gcacaaagt gatgaagac 
      tgggaagtt cacgtgagt gaggtgaag accagcgat 
      atgcacatt ttagaacca attaatttg aaagtcaat 
      ctcgcaact tgtatgatc actgatgat ccaaagttg 
      ccacatatg aagatttca ggggaatta ccctcgatt 
      attgttaat gtttctgat gcccgattg ttactactg 
      atggcgttg gggaatagc attccttta ccccaaagt 
      gacattccg gaacctaca cctttagcg aaagttgca 
      aaacgggag tcgagcatg atgttgaga tatttggaa 
      atgcaagaa aaagcgtcg aaaacacaa actctgctc 
      ccctccaca gcgaaaaaa accgacgac caattggta 
      caattcacg acgctagag atacatttt gcaatgtcc 
      gaattatta ctcaacgtg aagcatcaa agcgatatt 
      gcgtctccc atcacaaac atcgcttgt ttcagtgtt 
      aaatcactc gagcttggt ttggtgcaa caaacctac 
      aatacgaaa atcactcta gatttgggt ggtgtcagt 
      ctgattcaa acacacgac tcgaaagaa atcaaaatg 
      ataagttcg ccgagtttc gaagaggat gaacaatac 
      ctcttccaa attaaattc actcaggtt aacaaaaat 
      tcacccgag tttcactcg acttacaaa tcgtgtgag 
      gcaagtctt gcgttggag tttgcaaaa cttgggatt 
      attttgcac aaggagggt ttgctgtcc ttgattcag 
      tttgcaacg agtctgcag actgagatt gatgatatt 
      tgggggagt agaccggtt gagagggtc gcttcgact 
      catacacca agttttaag agccagttg tcgacgata 
      agtgaggga ttgggctcc caattggcc cctcaagaa 
      aaaaagagg aaacataag gaaaccaag attgtcgtt 
      gagaccatc aagtttaaa ttatcggcg aatgtgcaa 
      gaagtgaac gttgaattc gcgactgaa cacgaaagg 
      atttcgttt tgggccctt aagggcgta cactcgaac 
      atcatcgtc aaggagccc tacacgcaa atcaacgcc 
      aacttgaat caaatcgtt gtagttgat ttgaatccg 
      aaaacgatt cacaacttg attttgact gtcgaaggg 
      actgaagct ttatcaacc cgtatcgtg ttatacaat 
      ctcgataaa atgtccgaa agtgataag tcggatatc 
      tcagtcgat ttgacaatg ggtggtttg aggataatc 
      ttcctcaat tggtttgtg acccgaatg ttggacttt 
      ttgaacgaa ttccaaacc gcccaagct gctataatc 
      gaagcctct caagcggct gcgaaaaca gccaaagag 
      aacatgcaa aatgtttac gaaaaagca acgaaatta 
      tcactcagt atcaatctc aaagccccc gatataata 
      attcccgag aattcgaaa agttacgac gctattttg 
      ctggatttg ggtcacatt tccatttcc aatcaattc 
      ctaactatg gatatcagg aacgaacaa aatttttcg 
      gctgttatc gacgaaatg aagctaaat cttacggac 
      atgaagttg tcacgcgtt aaattaacc acgcgtgac 
      gaaattgat aaagaatta acacttttg gtgcctttg 
      acgtttaaa ttgaccatt aagaggaat ctatcgact 
      tcatggtac aaagcggtg cccgatatt gatattaca 
      ggtcgcatt ttttccatt acggttcta ctaagccaa 
      cttgattat aaaacaatc atgagtgtc ctcaatggt 
      aatttaagt gaagggcag aaaaaagga gctccggct 
      aaacaaccc cccaaagaa gtaattgaa gggacgaag 
      gtgatttct gatgacaaa gccttaatc acgattcat 
      gaaaaacaa tcggggcac gaggaaaag gacgcaaaa 
      acgaacacg ttcttgcgt ttgactttt acaatggat 
      aatctcgtg atgacgcta ttcaccggt ggttcaaaa 
      gcgttgtcc agtgattcg ccggctcac gacgaacgc 
      cacgccttg gctcgtttc tcattggag ggagtttcg 
      gtgaaagtt cgaatgttg acagataat tcgatgata 
      gcgtcgttc ctacttctc aactgcctt cttgacgat 
      atgagagta gggcgtgaa gggaaatta aatcgtctt 
      gtacaaagg agtcaagac tttggagca gcaccgtcg 
      gttagtatg gtttcgatt tcggaaact tccgcttcg 
      aaaagtatg atcgatatt gttgtccaa atgaaagac 
      aaagatatt ttcaccgac gtgagggtg tatagtttt 
      acccttatt ttatccgtc gattatctt atgaaaatt 
      gctgacttt ttaacgatt ccacaagaa gaagtcaaa 
      gaagcgcca aaagctagt aagagtaac acgtcgacg 
      aaatcggca atttcttcc ggaatgatg gaaatggag 
      gaaaaagcg tcccaaatt accctcaat ttgaagatc 
      gagaaaccg gatattgtg ttggtcgag catatggac 
      agtatcgac acacaagcc atgatctta aatagcgaa 
      gtggtaatg aaattacgt atgtcgggc gaacaccaa 
      gtcataaat ggttcgatt aaagattta caattgtat 
      acttgtaac tacaaccaa ctgaaacga attgatact 
      aaaagtaac gttttgcat cctgttact ataagtctc 
      gcaggatcg acgcctgaa aataaagga ctgcatctc 
      gagctttta gtcactgat attcgtctc agtgtctca 
      cctgccacc atagaactg ttgagtaga gttacagca 
      actgcgacc ggaagcagt gctgaacga acaagtgaa 
      gatgaagaa ttacaagac cattccgat gtgtgggaa 
      cacaaatcg tttgaggat gaggattac tggtttttc 
      agaaatgat gaggcggtc gaagcgacc gaagccttg 
      agcatctca tcatccatc gtcccgaag tcccccaac 
      aaaacccaa gaaatgtgc ataatctca atgccttca 
      ataatccta acaatcgaa gctggtgtc ggcagtaaa 
      accttccca atgctagtc ctcgaaagc agtctcaaa 
      ggccacgta aacaactgg agttcgcaa ttgagcgtc 
      gaagccggc ttaaccctt caaatgaac tactacaac 
      agccgttta gccctgtgg gaaccctta attgaacca 
      gttgaagtg tcccacagc aacaaaccc aaatgggtc 
      ccttgggaa ctcaaactg gaagtttcg cgaaacgaa 
      ccggaggaa gtcacaact ccgaccgat ctcgaatcg 
      ctagatagc gctccacag caagctgcg atgtgtatt 
      gatgtgtcc tccgaccat agtattgaa attgtggtc 
      actaagacg tgtctcgaa gtgttgaat aatctgggc 
      aaggcgttc gcaactgct atgaagccg ggcgaagtg 
      aagatgctt gagacgatg gcgccgttc aaaattgtg 
      aacgagttg gggtgttcg gtttgtgtt aatttggag 
      agggggtcg tttgcactg tttgggggc gaagaactg 
      gcggaagtt attttggag agtggggct gaggcacaa 
      ttgcagaaa aaaccaatg gtgctcagt tcaccctca 
      agactgggc ttgcaatta acaaccgat gcgctttcg 
      gttaaaaat tactttgtc aatgtgaaa gtcattgat 
      aagaattgc aacattgat ttgcccgtg gtgagggct 
      gataagcgg tttttccct ttgaattat cgaggggac 
      gggaacgat cactggggg ctcatttcg gacgttaca 
      gtcgacgaa ggtgtgact attattacg ttacgaagc 
      attttaaaa gtttacaac cattttaat atcccgatt 
      gaagtttat tacatgacg gtcagaggc aatgagctt 
      gaatcaatc acaacgatt cagcccgat tcacacgtt 
      aatattcca ctgaaagcc gtctataca ccgacgaac 
      gaattgttc ttcagtgtt gggggttat tcggttact 
      acagctcca tacatatgg aaagactta caaaccaac 
      ttaaccatc actaaaact ttgcaatgt caaccgaag 
      gaagccgaa ctagggaac gaccctttc attattcag 
      gcagttggc gaaatggag caggtttat tacgaaaac 
      acgtcaaga catacgatg tcaagcact tgttataac 
      attcatttg agaccggct gtagttttc aaaaattgt 
      ctaccgatt gatatcatt tgttgcatt gaaaacaca 
      gtttctgaa ataccggtc gagcctgga aaaacactt 
      ttgttaccg aatgttgaa cctggaaga tccgctcta 
      gtgattagg gtaagtttt aagtacgga taccgataa
    mRNA sequence
    • >TC004440 RA
      attgtcaaa aaatccaaa gtgggtcgg aaattcgtt 
      tggagtctg tttcagaaa aataaacgt aattagttt 
      gattaatcg ataaaggag tcaaccgtg atattggct 
      gtttgggtc aagctgtga gatggattt acaaatagc 
      cattggttg atgtcatct ccctttata gcatttttg 
      ctatagcag aagcaagta ggagtgcat gcaagagca 
      ctcctgctt tccttatct attgtaaat agaccgttg 
      ataataaac aatggtgtt tgaaagcgt attaactca 
      ggtattaaa ccgcttcct tggtgccta tgtagagaa 
      tttagacac aaatcaact aagagtggg aatatgggg 
      aggcgacgt cgaattaaa ggatttggt tttgaagca 
      gtccgcttt agacgactt ggacctccc tcttcaaac 
      tatctacgg cagaatagg gaagctagt tttgaaaat 
      accgtggaa gaatttata tggggctgc tttcgttat 
      aaacattga agatatcta tcttcttgc agccccaaa 
      ccaacaagt tatctacaa tgaagagaa agaagaaat 
      caaaaagtc ggaagccaa gaaacgaga gattcttaa 
      agttgaatt ggcgaaaaa acaagaagc tgacaagga 
      caaacctaa agctgacaa cacatttgt ggagaaatt 
      agtgacaca aatcatcaa aaatatcca attaaatat 
      caaaaatat tcatattag gtatgagga taaggtcac 
      aagtcctac accttttgc gttaggaat cacactgag 
      tgatttaca tgttgtgag cactgatga gaactggaa 
      acctgccat aacgaaaga agatatcac aaagatctt 
      taaaattgt tacactgga tggtttagc gatttactg 
      gaattcaaa caccgcttg ctacgatgg actccctaa 
      agtcgacat gttacacaa aatgtgtac agaaattgc 
      atcaaaaaa taatacacc cattaatta caagtatgt 
      tttgggacc aatcaacgc ctcggctcg cctccgcat 
      caaccagcg ccctgaact cgacaaacc cgctttcac 
      tattcccaa aatccactt aaatctcga catggaacg 
      ccttttcgt tggcataac caaagccca atatcgcga 
      tattatagc tcttggtga ttccatgga ccgcatgat 
      gaagggaac cccctaccg caaattccg ccccaacgt 
      gccttccta caaaggcaa ctacaaaac gtggtggca 
      tttcgcgta caattgcgt gctggaagg cgaagtcag 
      gcggaggag acgcaattg ggattggaa ccacatttg 
      caaacatcg cgatttgtg tcgggaata tgggactct 
      atacgagaa aaaactgca aaataaggc gaccagtga 
      tgagttgag tcgtatcga agagtgcga gaaagtgat 
      cgatgtctt caatattgt tattataag acagagggt 
      tgagtttga gaatgagag acgggtgaa gactaaagg 
      gccagaaaa gggttggtt tgggtggat gtgggggag 
      taaaacttc aatcgattc ggatagtct agagtctga 
      tagtggcac cgaactcag gaaacaatt ccaaaatgc 
      gatgaccaa agaggagaa agaacggct gtataaggc 
      tataggtta tcaggaaaa tgcgttacc gtctgagtt 
      tcctgaggc ttacgtcga caattcatg cacttttat 
      tctacgaac gcttgaagt cgaattaag tgacgactc 
      aggaaacgt caaaagggt tgtttttac tgaacttaa 
      aggggtcaa gtgtcgcgt ggacaccag agcggcggc 
      caatgcctt aaagcttca tgtcaaaat cgatgagtt 
      ccaaacgtt tggtctcca acaagacga ttttttgcc 
      gaaaataat cacctcaga gactggaaa agacgacag 
      tgggttgct tcaagtgct cttcgagac caatcctct 
      cgataaaaa atgcggtca aagactgca tgtcacctc 
      ccaaccgct taaaatcgt ctacgatgc acaaactgt 
      cttgaaagc gattgaaat tttcaaagt tccgtcttc 
      gtcagcttt ggaccagat ttcggcagc ggccgattc 
      cggtctttc taatatgaa agaaatgac ttcgagtgg 
      tctccagca cgcgattga cacccacgt tcatctcga 
      tctcaaagt agattttcg tgcccctta cattataat 
      cccctatgg agggaaata caccgaaaa tgaaaatgt 
      cctagtggt caatttagg taatttcca aatgcactc 
      acttgatcg gccgccaag gagggatgt gaggcagat 
      gtatgccga agggtgcga cgagaagga gattttgac 
      acagctgat ggctcaaag ttacgatca gtttgcgtt 
      ggaattgac tcgagtgca aattctaat cgcacaaag 
      tgatgaaga ctgggaagt tcacgtgag tgaggtgaa 
      gaccagcga tatgcacat tttagaacc aattaattt 
      gaaagtcaa tctcgcaac ttgtatgat cactgatga 
      tccaaagtt gccacatat gaagatttc aggggaatt 
      accctcgat tattgttaa tgtttctga tgcccgatt 
      gttactact gatggcgtt ggggaatag cattccttt 
      accccaaag tgacattcc ggaacctac acctttagc 
      gaaagttgc aaaacggga gtcgagcat gatgttgag 
      atatttgga aatgcaaga aaaagcgtc gaaaacaca 
      aactctgct cccctccac agcgaaaaa aaccgacga 
      ccaattggt acaattcac gacgctaga gatacattt 
      tgcaatgtc cgaattatt actcaacgt gaagcatca 
      aagcgatat tgcgtctcc catcacaaa catcgcttg 
      tttcagtgt taaatcact cgagcttgg tttggtgca 
      acaaaccta caatacgaa aatcactct agatttggg 
      tggtgtcag tctgattca aacacacga ctcgaaaga 
      aatcaaaat gataagttc gccgagttt cgaagagga 
      tgaacaata cctcttcca aattaaatt cactcaggt 
      taacaaaaa ttcacccga gtttcactc gacttacaa 
      atcgtgtga ggcaagtct tgcgttgga gtttgcaaa 
      acttgggat tattttgca caaggaggg tttgctgtc 
      cttgattca gtttgcaac gagtctgca gactgagat 
      tgatgatat ttgggggag tagaccggt tgagagggt 
      cgcttcgac tcatacacc aagttttaa gagccagtt 
      gtcgacgat aagtgaggg attgggctc ccaattggc 
      ccctcaaga aaaaaagag gaaacataa ggaaaccaa 
      gattgtcgt tgagaccat caagtttaa attatcggc 
      gaatgtgca agaagtgaa cgttgaatt cgcgactga 
      acacgaaag gatttcgtt ttgggccct taagggcgt 
      acactcgaa catcatcgt caaggagcc ctacacgca 
      aatcaacgc caacttgaa tcaaatcgt tgtagttga 
      tttgaatcc gaaaacgat tcacaactt gattttgac 
      tgtcgaagg gactgaagc tttatcaac ccgtatcgt 
      gttatacaa tctcgataa aatgtccga aagtgataa 
      gtcggatat ctcagtcga tttgacaat gggtggttt 
      gaggataat cttcctcaa ttggtttgt gacccgaat 
      gttggactt tttgaacga attccaaac cgcccaagc 
      tgctataat cgaagcctc tcaagcggc tgcgaaaac 
      agccaaaga gaacatgca aaatgttta cgaaaaagc 
      aacgaaatt atcactcag tatcaatct caaagcccc 
      cgatataat aattcccga gaattcgaa aagttacga 
      cgctatttt gctggattt gggtcacat ttccatttc 
      caatcaatt cctaactat ggatatcag gaacgaaca 
      aaatttttc ggctgttat cgacgaaat gaagctaaa 
      tcttacgga catgaagtt gtcacgcgt taaattaac 
      cacgcgtga cgaaattga taaagaatt aacactttt 
      ggtgccttt gacgtttaa attgaccat taagaggaa 
      tctatcgac ttcatggta caaagcggt gcccgatat 
      tgatattac aggtcgcat tttttccat tacggttct 
      actaagcca acttgatta taaaacaat catgagtgt 
      cctcaatgg taatttaag tgaagggca gaaaaaagg 
      agctccggc taaacaacc ccccaaaga agtaattga 
      agggacgaa ggtgatttc tgatgacaa agccttaat 
      cacgattca tgaaaaaca atcggggca cgaggaaaa 
      ggacgcaaa aacgaacac gttcttgcg tttgacttt 
      tacaatgga taatctcgt gatgacgct attcaccgg 
      tggttcaaa agcgttgtc cagtgattc gccggctca 
      cgacgaacg ccacgcctt ggctcgttt ctcattgga 
      gggagtttc ggtgaaagt tcgaatgtt gacagataa 
      ttcgatgat agcgtcgtt cctacttct caactgcct 
      tcttgacga tatgagagt agggcgtga agggaaatt 
      aaatcgtct tgtacaaag gagtcaaga ctttggagc 
      agcaccgtc ggttagtat ggtttcgat ttcggaaac 
      ttccgcttc gaaaagtat gatcgatat tgttgtcca 
      aatgaaaga caaagatat tttcaccga cgtgagggt 
      gtatagttt tacccttat tttatccgt cgattatct 
      tatgaaaat tgctgactt tttaacgat tccacaaga 
      agaagtcaa agaagcgcc aaaagctag taagagtaa 
      cacgtcgac gaaatcggc aatttcttc cggaatgat 
      ggaaatgga ggaaaaagc gtcccaaat taccctcaa 
      tttgaagat cgagaaacc ggatattgt gttggtcga 
      gcatatgga cagtatcga cacacaagc catgatctt 
      aaatagcga agtggtaat gaaattacg tatgtcggg 
      cgaacacca agtcataaa tggttcgat taaagattt 
      acaattgta tacttgtaa ctacaacca actgaaacg 
      aattgatac taaaagtaa cgttttgca tcctgttac 
      tataagtct cgcaggatc gacgcctga aaataaagg 
      actgcatct cgagctttt agtcactga tattcgtct 
      cagtgtctc acctgccac catagaact gttgagtag 
      agttacagc aactgcgac cggaagcag tgctgaacg 
      aacaagtga agatgaaga attacaaga ccattccga 
      tgtgtggga acacaaatc gtttgagga tgaggatta 
      ctggttttt cagaaatga tgaggcggt cgaagcgac 
      cgaagcctt gagcatctc atcatccat cgtcccgaa 
      gtcccccaa caaaaccca agaaatgtg cataatctc 
      aatgccttc aataatcct aacaatcga agctggtgt 
      cggcagtaa aaccttccc aatgctagt cctcgaaag 
      cagtctcaa aggccacgt aaacaactg gagttcgca 
      attgagcgt cgaagccgg cttaaccct tcaaatgaa 
      ctactacaa cagccgttt agccctgtg ggaaccctt 
      aattgaacc agttgaagt gtcccacag caacaaacc 
      caaatgggt cccttggga actcaaact ggaagtttc 
      gcgaaacga accggagga agtcacaac tccgaccga 
      tctcgaatc gctagatag cgctccaca gcaagctgc 
      gatgtgtat tgatgtgtc ctccgacca tagtattga 
      aattgtggt cactaagac gtgtctcga agtgttgaa 
      taatctggg caaggcgtt cgcaactgc tatgaagcc 
      gggcgaagt gaagatgct tgagacgat ggcgccgtt 
      caaaattgt gaacgagtt ggggtgttc ggtttgtgt 
      taatttgga gagggggtc gtttgcact gtttggggg 
      cgaagaact ggcggaagt tattttgga gagtggggc 
      tgaggcaca attgcagaa aaaaccaat ggtgctcag 
      ttcaccctc aagactggg cttgcaatt aacaaccga 
      tgcgctttc ggttaaaaa ttactttgt caatgtgaa 
      agtcattga taagaattg caacattga tttgcccgt 
      ggtgagggc tgataagcg gtttttccc tttgaatta 
      tcgagggga cgggaacga tcactgggg gctcatttc 
      ggacgttac agtcgacga aggtgtgac tattattac 
      gttacgaag cattttaaa agtttacaa ccattttaa 
      tatcccgat tgaagttta ttacatgac ggtcagagg 
      caatgagct tgaatcaat cacaacgat tcagcccga 
      ttcacacgt taatattcc actgaaagc cgtctatac 
      accgacgaa cgaattgtt cttcagtgt tgggggtta 
      ttcggttac tacagctcc atacatatg gaaagactt 
      acaaaccaa cttaaccat cactaaaac tttgcaatg 
      tcaaccgaa ggaagccga actagggaa cgacccttt 
      cattattca ggcagttgg cgaaatgga gcaggttta 
      ttacgaaaa cacgtcaag acatacgat gtcaagcac 
      ttgttataa cattcattt gagaccggc tgtagtttt 
      caaaaattg tctaccgat tgatatcat ttgttgcat 
      tgaaaacac agtttctga aataccggt cgagcctgg 
      aaaaacact tttgttacc gaatgttga acctggaag 
      atccgctct agtgattag ggtaagttt taagtacgg 
      ataccgata aatacgacg ttctgcatt taacttaca 
      aaaaaatta taagtggat ggagcatta atttttgtt 
      tttctataa ctgtattaa aaaatcatg ccgatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY