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Gene information
General information
TC004420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5454308..5459475
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC004420
    iBeetle screen iB_03752

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004420 RA
      MSKKRGNKK NLNLESDNE DEGLVTKGE AEKGNITSK 
PSKSKAPKK GKKGKKDDW SDDDDDKTV SLIDNVDEE 
IQPVAKKSA KKKSKKKEH ESGSEDEED KVEVKVENK 
KKAGKKGKG KNDWSDNDS EVELQISDV EEEIQPVKK 
SSKKVAKTK KKKDLSEED EILEEPVEE SDKEEPDEI 
AEKVSELQI TEAKKEPEE KKLTHKEKK KLKKQQEYE 
KQMETLLKK GGQGHSDLD SNFTVSQSQ KTAGQLAQL 
ENAVDIKIE NFSISAKGN DLFVNANLL IANGRHYGL 
VGPNGHGKT TLLRHIAQR AFAIPPNID ILYCEQEVV 
ADDFSAVES VLKSDVKRT ELLEECKKL EDAFNTGDL 
GVQDRLNEV YAELKAIGA DSAEPKARR ILAGLGFTK 
EMQDRATKN FSGGWRMRV SLARALYIE PTLLLLDEP 
TNHLDLNAV IWLDNYLQG WKKTLLIVS HDQSFLDNV 
CNEIIHLDN QKLYYYKGN YSMFKKMYQ QKRKEMIKE 
YEKQEKRLK ELKAHGSSK KQAEKKQKE ALTRKQEKN 
RTKMQKQED ETAPTELLQ KPKEYFVKF SFPEPPPLQ 
PPILGLHNV TFAYSGQKP LFRNTDFGI DMSSRVAIV 
GPNGVGKST FLKLLTGDL DPQQGEVRK NHRLRIGRF 
DQHSGEHLT AEETPAEYL MRLFDLPYE KSRKQLGTF 
GLASHAHTI KMKDLSGGQ KARVALAEL CLNAPDVLI 
LDEPTNNLD IESIDALAE AINDFSGGV IIVSHDERL 
IRETDCALY VIEDQTINE LDGDFDDYR KELLESLGE 
VINSPSIAA NAAIAQ
    CDS sequence
    • >TC004420 RA
      atgtctaaa aaacgcgga aacaaaaag aatttaaat 
ttggaaagt gataacgag gacgaaggg cttgttacg 
aaaggagag gccgaaaaa ggtaacata acctcaaaa 
ccgagtaag agtaaagcc cccaaaaag ggtaaaaag 
ggcaaaaaa gacgactgg agcgatgat gacgatgat 
aaaacggtc tctctgatt gataacgtg gacgaggag 
attcaaccg gtggcgaaa aagagtgca aagaagaag 
tcgaaaaag aaggaacat gagtccggt agtgaagat 
gaagaagat aaagtcgag gttaaggtt gagaataaa 
aagaaagct ggaaaaaaa ggtaaaggt aaaaacgat 
tggtcggat aatgacagt gaagtggaa ctgcaaata 
tcggacgtt gaggaagaa attcaaccg gtgaagaaa 
agtagcaaa aaggttgct aaaaccaaa aagaagaaa 
gatctgagt gaggaagat gaaatcctt gaggaacca 
gttgaagag tcagacaaa gaagaacct gacgaaatc 
gcggaaaaa gtatcagaa ctgcaaata actgaagct 
aagaaggaa ccagaagag aagaaactc acccacaag 
gagaagaaa aagctgaag aagcagcaa gaatacgag 
aaacaaatg gaaaccctc cttaaaaaa ggtggccaa 
ggtcactca gaccttgac agtaacttc acagtctcc 
cagtcgcaa aaaaccgcc ggacaattg gcccaatta 
gaaaatgcc gttgatatt aaaatcgaa aatttcagt 
atctcggct aaaggcaac gatttattc gtaaacgcg 
aatttgtta atcgccaac gggcgtcat tacggtctt 
gtggggccc aatggtcac ggtaaaaca acacttttg 
cgtcatatt gcgcaacgg gctttcgcg ataccccca 
aatatcgat attttgtat tgtgaacaa gaagtggtc 
gctgatgat ttttcagcg gttgaatcg gtgcttaaa 
tcggatgtg aaacggact gaattgtta gaagaatgt 
aaaaaatta gaggatgcg tttaatacc ggggatttg 
ggcgttcag gaccggctt aatgaagtt tatgccgaa 
ttgaaggcc attggggcg gattcggcc gaacctaag 
gcgaggaga atcttagct ggtctagga ttcactaaa 
gaaatgcaa gatagagca actaaaaat ttttctggt 
ggatggaga atgagagtg tcactagcc agagcgttg 
tatatagaa cccacttta ttacttctt gatgaacca 
acgaatcat ttagatttg aatgcagtt atttggttg 
gataattat ttgcaaggg tggaaaaaa actctactt 
atcgtttca cacgaccag tcattctta gataatgtt 
tgtaatgaa attatccat ttagataat caaaagttg 
tattattac aaagggaat tactcaatg tttaaaaaa 
atgtaccaa caaaaacgc aaagaaatg attaaagaa 
tacgagaag caggaaaaa cgtttgaaa gagttaaag 
gcacacgga tcgtcgaaa aaacaagcc gaaaagaaa 
caaaaagag gctttgacg cgaaaacag gagaagaac 
agaacgaaa atgcagaaa caagaggac gaaacagcc 
ccaactgaa cttctacaa aaaccgaaa gaatatttc 
gtcaaattt tcatttccg gaacctccg cctctacaa 
cccccaatt ttggggctt cataacgta acttttgca 
tattcgggc caaaaaccc ctcttccga aacaccgac 
ttcggtatc gatatgagc agcagagtg gcaatagtt 
ggtccgaac ggagtcgga aaatccaca tttttgaaa 
ttattaacc ggtgatttg gacccgcaa caaggggaa 
gtccggaaa aatcatcgt ttgaggatt ggtcgtttt 
gatcaacat tcaggtgag catttaacg gccgaagaa 
acacccgct gaatatctg atgcgattg tttgatctg 
ccgtacgaa aaatcccga aagcaattg gggactttt 
ggtctagcg agtcatgct cacactatc aaaatgaag 
gacttgtcc gggggacag aaagcacga gtggcttta 
gccgaattg tgtcttaac gcaccggat gttctcatt 
ttggatgaa ccgacgaat aatttagat attgaatcg 
attgatgca ctagctgag gccattaac gacttcagt 
ggaggggtt attatagtg agccacgac gaacgactt 
atacgtgaa actgattgc gccttgtac gtaatagaa 
gaccagacc ataaacgaa ttagacggc gacttcgac 
gactaccgc aaagagctc ttggagagt ctgggtgaa 
gtcatcaac agcccaagt attgcagca aacgctgcc 
attgctcaa taa
    mRNA sequence
    • >TC004420 RA
      tttcggcga gtgacaacg catctgtca ctttcacgt 
gtaaaaatt gtgtaattt cgtgaaaag taattttaa 
gactttata accatgtct aaaaaacgc ggaaacaaa 
aagaattta aatttggaa agtgataac gaggacgaa 
gggcttgtt acgaaagga gaggccgaa aaaggtaac 
ataacctca aaaccgagt aagagtaaa gcccccaaa 
aagggtaaa aagggcaaa aaagacgac tggagcgat 
gatgacgat gataaaacg gtctctctg attgataac 
gtggacgag gagattcaa ccggtggcg aaaaagagt 
gcaaagaag aagtcgaaa aagaaggaa catgagtcc 
ggtagtgaa gatgaagaa gataaagtc gaggttaag 
gttgagaat aaaaagaaa gctggaaaa aaaggtaaa 
ggtaaaaac gattggtcg gataatgac agtgaagtg 
gaactgcaa atatcggac gttgaggaa gaaattcaa 
ccggtgaag aaaagtagc aaaaaggtt gctaaaacc 
aaaaagaag aaagatctg agtgaggaa gatgaaatc 
cttgaggaa ccagttgaa gagtcagac aaagaagaa 
cctgacgaa atcgcggaa aaagtatca gaactgcaa 
ataactgaa gctaagaag gaaccagaa gagaagaaa 
ctcacccac aaggagaag aaaaagctg aagaagcag 
caagaatac gagaaacaa atggaaacc ctccttaaa 
aaaggtggc caaggtcac tcagacctt gacagtaac 
ttcacagtc tcccagtcg caaaaaacc gccggacaa 
ttggcccaa ttagaaaat gccgttgat attaaaatc 
gaaaatttc agtatctcg gctaaaggc aacgattta 
ttcgtaaac gcgaatttg ttaatcgcc aacgggcgt 
cattacggt cttgtgggg cccaatggt cacggtaaa 
acaacactt ttgcgtcat attgcgcaa cgggctttc 
gcgataccc ccaaatatc gatattttg tattgtgaa 
caagaagtg gtcgctgat gatttttca gcggttgaa 
tcggtgctt aaatcggat gtgaaacgg actgaattg 
ttagaagaa tgtaaaaaa ttagaggat gcgtttaat 
accggggat ttgggcgtt caggaccgg cttaatgaa 
gtttatgcc gaattgaag gccattggg gcggattcg 
gccgaacct aaggcgagg agaatctta gctggtcta 
ggattcact aaagaaatg caagataga gcaactaaa 
aatttttct ggtggatgg agaatgaga gtgtcacta 
gccagagcg ttgtatata gaacccact ttattactt 
cttgatgaa ccaacgaat catttagat ttgaatgca 
gttatttgg ttggataat tatttgcaa gggtggaaa 
aaaactcta cttatcgtt tcacacgac cagtcattc 
ttagataat gtttgtaat gaaattatc catttagat 
aatcaaaag ttgtattat tacaaaggg aattactca 
atgtttaaa aaaatgtac caacaaaaa cgcaaagaa 
atgattaaa gaatacgag aagcaggaa aaacgtttg 
aaagagtta aaggcacac ggatcgtcg aaaaaacaa 
gccgaaaag aaacaaaaa gaggctttg acgcgaaaa 
caggagaag aacagaacg aaaatgcag aaacaagag 
gacgaaaca gccccaact gaacttcta caaaaaccg 
aaagaatat ttcgtcaaa ttttcattt ccggaacct 
ccgcctcta caaccccca attttgggg cttcataac 
gtaactttt gcatattcg ggccaaaaa cccctcttc 
cgaaacacc gacttcggt atcgatatg agcagcaga 
gtggcaata gttggtccg aacggagtc ggaaaatcc 
acatttttg aaattatta accggtgat ttggacccg 
caacaaggg gaagtccgg aaaaatcat cgtttgagg 
attggtcgt tttgatcaa cattcaggt gagcattta 
acggccgaa gaaacaccc gctgaatat ctgatgcga 
ttgtttgat ctgccgtac gaaaaatcc cgaaagcaa 
ttggggact tttggtcta gcgagtcat gctcacact 
atcaaaatg aaggacttg tccggggga cagaaagca 
cgagtggct ttagccgaa ttgtgtctt aacgcaccg 
gatgttctc attttggat gaaccgacg aataattta 
gatattgaa tcgattgat gcactagct gaggccatt 
aacgacttc agtggaggg gttattata gtgagccac 
gacgaacga cttatacgt gaaactgat tgcgccttg 
tacgtaata gaagaccag accataaac gaattagac 
ggcgacttc gacgactac cgcaaagag ctcttggag 
agtctgggt gaagtcatc aacagccca agtattgca 
gcaaacgct gccattgct caataatac aacaaaaaa 
tttctacaa tt
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