| General information | |||
| TC004420 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 5454308..5459475 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC004420 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03752 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC004420 RA
MSKKRGNKK NLNLESDNE DEGLVTKGE AEKGNITSK PSKSKAPKK GKKGKKDDW SDDDDDKTV SLIDNVDEE IQPVAKKSA KKKSKKKEH ESGSEDEED KVEVKVENK KKAGKKGKG KNDWSDNDS EVELQISDV EEEIQPVKK SSKKVAKTK KKKDLSEED EILEEPVEE SDKEEPDEI AEKVSELQI TEAKKEPEE KKLTHKEKK KLKKQQEYE KQMETLLKK GGQGHSDLD SNFTVSQSQ KTAGQLAQL ENAVDIKIE NFSISAKGN DLFVNANLL IANGRHYGL VGPNGHGKT TLLRHIAQR AFAIPPNID ILYCEQEVV ADDFSAVES VLKSDVKRT ELLEECKKL EDAFNTGDL GVQDRLNEV YAELKAIGA DSAEPKARR ILAGLGFTK EMQDRATKN FSGGWRMRV SLARALYIE PTLLLLDEP TNHLDLNAV IWLDNYLQG WKKTLLIVS HDQSFLDNV CNEIIHLDN QKLYYYKGN YSMFKKMYQ QKRKEMIKE YEKQEKRLK ELKAHGSSK KQAEKKQKE ALTRKQEKN RTKMQKQED ETAPTELLQ KPKEYFVKF SFPEPPPLQ PPILGLHNV TFAYSGQKP LFRNTDFGI DMSSRVAIV GPNGVGKST FLKLLTGDL DPQQGEVRK NHRLRIGRF DQHSGEHLT AEETPAEYL MRLFDLPYE KSRKQLGTF GLASHAHTI KMKDLSGGQ KARVALAEL CLNAPDVLI LDEPTNNLD IESIDALAE AINDFSGGV IIVSHDERL IRETDCALY VIEDQTINE LDGDFDDYR KELLESLGE VINSPSIAA NAAIAQ
- >TC004420 RA
atgtctaaa aaacgcgga aacaaaaag aatttaaat ttggaaagt gataacgag gacgaaggg cttgttacg aaaggagag gccgaaaaa ggtaacata acctcaaaa ccgagtaag agtaaagcc cccaaaaag ggtaaaaag ggcaaaaaa gacgactgg agcgatgat gacgatgat aaaacggtc tctctgatt gataacgtg gacgaggag attcaaccg gtggcgaaa aagagtgca aagaagaag tcgaaaaag aaggaacat gagtccggt agtgaagat gaagaagat aaagtcgag gttaaggtt gagaataaa aagaaagct ggaaaaaaa ggtaaaggt aaaaacgat tggtcggat aatgacagt gaagtggaa ctgcaaata tcggacgtt gaggaagaa attcaaccg gtgaagaaa agtagcaaa aaggttgct aaaaccaaa aagaagaaa gatctgagt gaggaagat gaaatcctt gaggaacca gttgaagag tcagacaaa gaagaacct gacgaaatc gcggaaaaa gtatcagaa ctgcaaata actgaagct aagaaggaa ccagaagag aagaaactc acccacaag gagaagaaa aagctgaag aagcagcaa gaatacgag aaacaaatg gaaaccctc cttaaaaaa ggtggccaa ggtcactca gaccttgac agtaacttc acagtctcc cagtcgcaa aaaaccgcc ggacaattg gcccaatta gaaaatgcc gttgatatt aaaatcgaa aatttcagt atctcggct aaaggcaac gatttattc gtaaacgcg aatttgtta atcgccaac gggcgtcat tacggtctt gtggggccc aatggtcac ggtaaaaca acacttttg cgtcatatt gcgcaacgg gctttcgcg ataccccca aatatcgat attttgtat tgtgaacaa gaagtggtc gctgatgat ttttcagcg gttgaatcg gtgcttaaa tcggatgtg aaacggact gaattgtta gaagaatgt aaaaaatta gaggatgcg tttaatacc ggggatttg ggcgttcag gaccggctt aatgaagtt tatgccgaa ttgaaggcc attggggcg gattcggcc gaacctaag gcgaggaga atcttagct ggtctagga ttcactaaa gaaatgcaa gatagagca actaaaaat ttttctggt ggatggaga atgagagtg tcactagcc agagcgttg tatatagaa cccacttta ttacttctt gatgaacca acgaatcat ttagatttg aatgcagtt atttggttg gataattat ttgcaaggg tggaaaaaa actctactt atcgtttca cacgaccag tcattctta gataatgtt tgtaatgaa attatccat ttagataat caaaagttg tattattac aaagggaat tactcaatg tttaaaaaa atgtaccaa caaaaacgc aaagaaatg attaaagaa tacgagaag caggaaaaa cgtttgaaa gagttaaag gcacacgga tcgtcgaaa aaacaagcc gaaaagaaa caaaaagag gctttgacg cgaaaacag gagaagaac agaacgaaa atgcagaaa caagaggac gaaacagcc ccaactgaa cttctacaa aaaccgaaa gaatatttc gtcaaattt tcatttccg gaacctccg cctctacaa cccccaatt ttggggctt cataacgta acttttgca tattcgggc caaaaaccc ctcttccga aacaccgac ttcggtatc gatatgagc agcagagtg gcaatagtt ggtccgaac ggagtcgga aaatccaca tttttgaaa ttattaacc ggtgatttg gacccgcaa caaggggaa gtccggaaa aatcatcgt ttgaggatt ggtcgtttt gatcaacat tcaggtgag catttaacg gccgaagaa acacccgct gaatatctg atgcgattg tttgatctg ccgtacgaa aaatcccga aagcaattg gggactttt ggtctagcg agtcatgct cacactatc aaaatgaag gacttgtcc gggggacag aaagcacga gtggcttta gccgaattg tgtcttaac gcaccggat gttctcatt ttggatgaa ccgacgaat aatttagat attgaatcg attgatgca ctagctgag gccattaac gacttcagt ggaggggtt attatagtg agccacgac gaacgactt atacgtgaa actgattgc gccttgtac gtaatagaa gaccagacc ataaacgaa ttagacggc gacttcgac gactaccgc aaagagctc ttggagagt ctgggtgaa gtcatcaac agcccaagt attgcagca aacgctgcc attgctcaa taa
- >TC004420 RA
tttcggcga gtgacaacg catctgtca ctttcacgt gtaaaaatt gtgtaattt cgtgaaaag taattttaa gactttata accatgtct aaaaaacgc ggaaacaaa aagaattta aatttggaa agtgataac gaggacgaa gggcttgtt acgaaagga gaggccgaa aaaggtaac ataacctca aaaccgagt aagagtaaa gcccccaaa aagggtaaa aagggcaaa aaagacgac tggagcgat gatgacgat gataaaacg gtctctctg attgataac gtggacgag gagattcaa ccggtggcg aaaaagagt gcaaagaag aagtcgaaa aagaaggaa catgagtcc ggtagtgaa gatgaagaa gataaagtc gaggttaag gttgagaat aaaaagaaa gctggaaaa aaaggtaaa ggtaaaaac gattggtcg gataatgac agtgaagtg gaactgcaa atatcggac gttgaggaa gaaattcaa ccggtgaag aaaagtagc aaaaaggtt gctaaaacc aaaaagaag aaagatctg agtgaggaa gatgaaatc cttgaggaa ccagttgaa gagtcagac aaagaagaa cctgacgaa atcgcggaa aaagtatca gaactgcaa ataactgaa gctaagaag gaaccagaa gagaagaaa ctcacccac aaggagaag aaaaagctg aagaagcag caagaatac gagaaacaa atggaaacc ctccttaaa aaaggtggc caaggtcac tcagacctt gacagtaac ttcacagtc tcccagtcg caaaaaacc gccggacaa ttggcccaa ttagaaaat gccgttgat attaaaatc gaaaatttc agtatctcg gctaaaggc aacgattta ttcgtaaac gcgaatttg ttaatcgcc aacgggcgt cattacggt cttgtgggg cccaatggt cacggtaaa acaacactt ttgcgtcat attgcgcaa cgggctttc gcgataccc ccaaatatc gatattttg tattgtgaa caagaagtg gtcgctgat gatttttca gcggttgaa tcggtgctt aaatcggat gtgaaacgg actgaattg ttagaagaa tgtaaaaaa ttagaggat gcgtttaat accggggat ttgggcgtt caggaccgg cttaatgaa gtttatgcc gaattgaag gccattggg gcggattcg gccgaacct aaggcgagg agaatctta gctggtcta ggattcact aaagaaatg caagataga gcaactaaa aatttttct ggtggatgg agaatgaga gtgtcacta gccagagcg ttgtatata gaacccact ttattactt cttgatgaa ccaacgaat catttagat ttgaatgca gttatttgg ttggataat tatttgcaa gggtggaaa aaaactcta cttatcgtt tcacacgac cagtcattc ttagataat gtttgtaat gaaattatc catttagat aatcaaaag ttgtattat tacaaaggg aattactca atgtttaaa aaaatgtac caacaaaaa cgcaaagaa atgattaaa gaatacgag aagcaggaa aaacgtttg aaagagtta aaggcacac ggatcgtcg aaaaaacaa gccgaaaag aaacaaaaa gaggctttg acgcgaaaa caggagaag aacagaacg aaaatgcag aaacaagag gacgaaaca gccccaact gaacttcta caaaaaccg aaagaatat ttcgtcaaa ttttcattt ccggaacct ccgcctcta caaccccca attttgggg cttcataac gtaactttt gcatattcg ggccaaaaa cccctcttc cgaaacacc gacttcggt atcgatatg agcagcaga gtggcaata gttggtccg aacggagtc ggaaaatcc acatttttg aaattatta accggtgat ttggacccg caacaaggg gaagtccgg aaaaatcat cgtttgagg attggtcgt tttgatcaa cattcaggt gagcattta acggccgaa gaaacaccc gctgaatat ctgatgcga ttgtttgat ctgccgtac gaaaaatcc cgaaagcaa ttggggact tttggtcta gcgagtcat gctcacact atcaaaatg aaggacttg tccggggga cagaaagca cgagtggct ttagccgaa ttgtgtctt aacgcaccg gatgttctc attttggat gaaccgacg aataattta gatattgaa tcgattgat gcactagct gaggccatt aacgacttc agtggaggg gttattata gtgagccac gacgaacga cttatacgt gaaactgat tgcgccttg tacgtaata gaagaccag accataaac gaattagac ggcgacttc gacgactac cgcaaagag ctcttggag agtctgggt gaagtcatc aacagccca agtattgca gcaaacgct gccattgct caataatac aacaaaaaa tttctacaa tt
