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Gene information
General information
TC004420 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 5454308..5459475
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004420
    iBeetle screen iB_03752

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004420 RA
      MSKKRGNKK NLNLESDNE DEGLVTKGE AEKGNITSK 
      PSKSKAPKK GKKGKKDDW SDDDDDKTV SLIDNVDEE 
      IQPVAKKSA KKKSKKKEH ESGSEDEED KVEVKVENK 
      KKAGKKGKG KNDWSDNDS EVELQISDV EEEIQPVKK 
      SSKKVAKTK KKKDLSEED EILEEPVEE SDKEEPDEI 
      AEKVSELQI TEAKKEPEE KKLTHKEKK KLKKQQEYE 
      KQMETLLKK GGQGHSDLD SNFTVSQSQ KTAGQLAQL 
      ENAVDIKIE NFSISAKGN DLFVNANLL IANGRHYGL 
      VGPNGHGKT TLLRHIAQR AFAIPPNID ILYCEQEVV 
      ADDFSAVES VLKSDVKRT ELLEECKKL EDAFNTGDL 
      GVQDRLNEV YAELKAIGA DSAEPKARR ILAGLGFTK 
      EMQDRATKN FSGGWRMRV SLARALYIE PTLLLLDEP 
      TNHLDLNAV IWLDNYLQG WKKTLLIVS HDQSFLDNV 
      CNEIIHLDN QKLYYYKGN YSMFKKMYQ QKRKEMIKE 
      YEKQEKRLK ELKAHGSSK KQAEKKQKE ALTRKQEKN 
      RTKMQKQED ETAPTELLQ KPKEYFVKF SFPEPPPLQ 
      PPILGLHNV TFAYSGQKP LFRNTDFGI DMSSRVAIV 
      GPNGVGKST FLKLLTGDL DPQQGEVRK NHRLRIGRF 
      DQHSGEHLT AEETPAEYL MRLFDLPYE KSRKQLGTF 
      GLASHAHTI KMKDLSGGQ KARVALAEL CLNAPDVLI 
      LDEPTNNLD IESIDALAE AINDFSGGV IIVSHDERL 
      IRETDCALY VIEDQTINE LDGDFDDYR KELLESLGE 
      VINSPSIAA NAAIAQ
    CDS sequence
    • >TC004420 RA
      atgtctaaa aaacgcgga aacaaaaag aatttaaat 
      ttggaaagt gataacgag gacgaaggg cttgttacg 
      aaaggagag gccgaaaaa ggtaacata acctcaaaa 
      ccgagtaag agtaaagcc cccaaaaag ggtaaaaag 
      ggcaaaaaa gacgactgg agcgatgat gacgatgat 
      aaaacggtc tctctgatt gataacgtg gacgaggag 
      attcaaccg gtggcgaaa aagagtgca aagaagaag 
      tcgaaaaag aaggaacat gagtccggt agtgaagat 
      gaagaagat aaagtcgag gttaaggtt gagaataaa 
      aagaaagct ggaaaaaaa ggtaaaggt aaaaacgat 
      tggtcggat aatgacagt gaagtggaa ctgcaaata 
      tcggacgtt gaggaagaa attcaaccg gtgaagaaa 
      agtagcaaa aaggttgct aaaaccaaa aagaagaaa 
      gatctgagt gaggaagat gaaatcctt gaggaacca 
      gttgaagag tcagacaaa gaagaacct gacgaaatc 
      gcggaaaaa gtatcagaa ctgcaaata actgaagct 
      aagaaggaa ccagaagag aagaaactc acccacaag 
      gagaagaaa aagctgaag aagcagcaa gaatacgag 
      aaacaaatg gaaaccctc cttaaaaaa ggtggccaa 
      ggtcactca gaccttgac agtaacttc acagtctcc 
      cagtcgcaa aaaaccgcc ggacaattg gcccaatta 
      gaaaatgcc gttgatatt aaaatcgaa aatttcagt 
      atctcggct aaaggcaac gatttattc gtaaacgcg 
      aatttgtta atcgccaac gggcgtcat tacggtctt 
      gtggggccc aatggtcac ggtaaaaca acacttttg 
      cgtcatatt gcgcaacgg gctttcgcg ataccccca 
      aatatcgat attttgtat tgtgaacaa gaagtggtc 
      gctgatgat ttttcagcg gttgaatcg gtgcttaaa 
      tcggatgtg aaacggact gaattgtta gaagaatgt 
      aaaaaatta gaggatgcg tttaatacc ggggatttg 
      ggcgttcag gaccggctt aatgaagtt tatgccgaa 
      ttgaaggcc attggggcg gattcggcc gaacctaag 
      gcgaggaga atcttagct ggtctagga ttcactaaa 
      gaaatgcaa gatagagca actaaaaat ttttctggt 
      ggatggaga atgagagtg tcactagcc agagcgttg 
      tatatagaa cccacttta ttacttctt gatgaacca 
      acgaatcat ttagatttg aatgcagtt atttggttg 
      gataattat ttgcaaggg tggaaaaaa actctactt 
      atcgtttca cacgaccag tcattctta gataatgtt 
      tgtaatgaa attatccat ttagataat caaaagttg 
      tattattac aaagggaat tactcaatg tttaaaaaa 
      atgtaccaa caaaaacgc aaagaaatg attaaagaa 
      tacgagaag caggaaaaa cgtttgaaa gagttaaag 
      gcacacgga tcgtcgaaa aaacaagcc gaaaagaaa 
      caaaaagag gctttgacg cgaaaacag gagaagaac 
      agaacgaaa atgcagaaa caagaggac gaaacagcc 
      ccaactgaa cttctacaa aaaccgaaa gaatatttc 
      gtcaaattt tcatttccg gaacctccg cctctacaa 
      cccccaatt ttggggctt cataacgta acttttgca 
      tattcgggc caaaaaccc ctcttccga aacaccgac 
      ttcggtatc gatatgagc agcagagtg gcaatagtt 
      ggtccgaac ggagtcgga aaatccaca tttttgaaa 
      ttattaacc ggtgatttg gacccgcaa caaggggaa 
      gtccggaaa aatcatcgt ttgaggatt ggtcgtttt 
      gatcaacat tcaggtgag catttaacg gccgaagaa 
      acacccgct gaatatctg atgcgattg tttgatctg 
      ccgtacgaa aaatcccga aagcaattg gggactttt 
      ggtctagcg agtcatgct cacactatc aaaatgaag 
      gacttgtcc gggggacag aaagcacga gtggcttta 
      gccgaattg tgtcttaac gcaccggat gttctcatt 
      ttggatgaa ccgacgaat aatttagat attgaatcg 
      attgatgca ctagctgag gccattaac gacttcagt 
      ggaggggtt attatagtg agccacgac gaacgactt 
      atacgtgaa actgattgc gccttgtac gtaatagaa 
      gaccagacc ataaacgaa ttagacggc gacttcgac 
      gactaccgc aaagagctc ttggagagt ctgggtgaa 
      gtcatcaac agcccaagt attgcagca aacgctgcc 
      attgctcaa taa
    mRNA sequence
    • >TC004420 RA
      tttcggcga gtgacaacg catctgtca ctttcacgt 
      gtaaaaatt gtgtaattt cgtgaaaag taattttaa 
      gactttata accatgtct aaaaaacgc ggaaacaaa 
      aagaattta aatttggaa agtgataac gaggacgaa 
      gggcttgtt acgaaagga gaggccgaa aaaggtaac 
      ataacctca aaaccgagt aagagtaaa gcccccaaa 
      aagggtaaa aagggcaaa aaagacgac tggagcgat 
      gatgacgat gataaaacg gtctctctg attgataac 
      gtggacgag gagattcaa ccggtggcg aaaaagagt 
      gcaaagaag aagtcgaaa aagaaggaa catgagtcc 
      ggtagtgaa gatgaagaa gataaagtc gaggttaag 
      gttgagaat aaaaagaaa gctggaaaa aaaggtaaa 
      ggtaaaaac gattggtcg gataatgac agtgaagtg 
      gaactgcaa atatcggac gttgaggaa gaaattcaa 
      ccggtgaag aaaagtagc aaaaaggtt gctaaaacc 
      aaaaagaag aaagatctg agtgaggaa gatgaaatc 
      cttgaggaa ccagttgaa gagtcagac aaagaagaa 
      cctgacgaa atcgcggaa aaagtatca gaactgcaa 
      ataactgaa gctaagaag gaaccagaa gagaagaaa 
      ctcacccac aaggagaag aaaaagctg aagaagcag 
      caagaatac gagaaacaa atggaaacc ctccttaaa 
      aaaggtggc caaggtcac tcagacctt gacagtaac 
      ttcacagtc tcccagtcg caaaaaacc gccggacaa 
      ttggcccaa ttagaaaat gccgttgat attaaaatc 
      gaaaatttc agtatctcg gctaaaggc aacgattta 
      ttcgtaaac gcgaatttg ttaatcgcc aacgggcgt 
      cattacggt cttgtgggg cccaatggt cacggtaaa 
      acaacactt ttgcgtcat attgcgcaa cgggctttc 
      gcgataccc ccaaatatc gatattttg tattgtgaa 
      caagaagtg gtcgctgat gatttttca gcggttgaa 
      tcggtgctt aaatcggat gtgaaacgg actgaattg 
      ttagaagaa tgtaaaaaa ttagaggat gcgtttaat 
      accggggat ttgggcgtt caggaccgg cttaatgaa 
      gtttatgcc gaattgaag gccattggg gcggattcg 
      gccgaacct aaggcgagg agaatctta gctggtcta 
      ggattcact aaagaaatg caagataga gcaactaaa 
      aatttttct ggtggatgg agaatgaga gtgtcacta 
      gccagagcg ttgtatata gaacccact ttattactt 
      cttgatgaa ccaacgaat catttagat ttgaatgca 
      gttatttgg ttggataat tatttgcaa gggtggaaa 
      aaaactcta cttatcgtt tcacacgac cagtcattc 
      ttagataat gtttgtaat gaaattatc catttagat 
      aatcaaaag ttgtattat tacaaaggg aattactca 
      atgtttaaa aaaatgtac caacaaaaa cgcaaagaa 
      atgattaaa gaatacgag aagcaggaa aaacgtttg 
      aaagagtta aaggcacac ggatcgtcg aaaaaacaa 
      gccgaaaag aaacaaaaa gaggctttg acgcgaaaa 
      caggagaag aacagaacg aaaatgcag aaacaagag 
      gacgaaaca gccccaact gaacttcta caaaaaccg 
      aaagaatat ttcgtcaaa ttttcattt ccggaacct 
      ccgcctcta caaccccca attttgggg cttcataac 
      gtaactttt gcatattcg ggccaaaaa cccctcttc 
      cgaaacacc gacttcggt atcgatatg agcagcaga 
      gtggcaata gttggtccg aacggagtc ggaaaatcc 
      acatttttg aaattatta accggtgat ttggacccg 
      caacaaggg gaagtccgg aaaaatcat cgtttgagg 
      attggtcgt tttgatcaa cattcaggt gagcattta 
      acggccgaa gaaacaccc gctgaatat ctgatgcga 
      ttgtttgat ctgccgtac gaaaaatcc cgaaagcaa 
      ttggggact tttggtcta gcgagtcat gctcacact 
      atcaaaatg aaggacttg tccggggga cagaaagca 
      cgagtggct ttagccgaa ttgtgtctt aacgcaccg 
      gatgttctc attttggat gaaccgacg aataattta 
      gatattgaa tcgattgat gcactagct gaggccatt 
      aacgacttc agtggaggg gttattata gtgagccac 
      gacgaacga cttatacgt gaaactgat tgcgccttg 
      tacgtaata gaagaccag accataaac gaattagac 
      ggcgacttc gacgactac cgcaaagag ctcttggag 
      agtctgggt gaagtcatc aacagccca agtattgca 
      gcaaacgct gccattgct caataatac aacaaaaaa 
      tttctacaa tt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY