General information | |||
TC004410 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 19811219..19815278 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC004410 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03748 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC004410 RA
MILKIFLEC LVAHLAYSL SQSPILIVV SYDAFRYDF FDTKSVPHM NRLRKLGTY SDYLINVFP TKTFPNHHS IATGLYPDI HGVVGNSYF NKSTNSVVH INYEMFHFD EKVVPIWRQ NEDAGKGRH SGVVMWPGG CYPYQGKQV TYCKDYDHN FDWFKRVDL VIGWIKDPK KPANLIMLY FDEPDTDGH IYGINSDVV NKLINKLDN ITLYLQQQL EKNNLTGKV TVVHLSDHG MLDVKPPNF INVTQYLTP GTYTYAGST PVLQFHPKK GFETEVYQK LKNASSQDH FTVYKKEEF LPRWHYKDN PRSPPILVL AEVGYGLDD LIISAKQNA EKNHYKLTN QSTFGVHGY DYTVKEMRP FFMARGPKI KVNHKVPPF NTVDLFNFF CAILEIAPT KNNGSLANV EDIFVGTSG YSLTTILTI TAGGIVVSL ILISCAAVL TLMMIKRQQ SITTTAALN KRFPQTFQT SIEAQHLLE PEDA
- >TC004410 RA
atgatttta aaaatattt ttagaatgt ctcgtggca cacttggca tattccctc tcccagagc ccgattcta atcgttgtg tcgtatgac gccttcagg tatgacttt ttcgacaca aaatctgtg ccccatatg aacagattg cgaaaattg gggacctac tctgactat ttgattaac gtattcccg accaaaacg ttcccaaat caccattca atcgccacg ggcttgtac cccgacatc cacggggtg gtgggtaac agttacttc aataaatca acaaatagt gtggttcat attaattat gaaatgttc cactttgat gagaaagtt gtcccgatt tggcggcaa aacgaggat gcgggcaag gggaggcac tcgggggtt gtgatgtgg ccaggtggt tgctacccc tatcaaggg aaacaagtg acgtattgt aaagattac gaccacaat tttgattgg tttaagcgc gttgatttg gtgattggg tggatcaaa gacccgaaa aagccggct aatttaata atgctctat tttgatgag ccagacact gatggacac atttacggc attaactcg gatgtagtc aataagttg ataaataaa ttggacaat attacgctt tatttgcaa caacaactt gaaaaaaat aatttgacg ggtaaagtc acagtggtg catctaagt gaccatggg atgttagat gtgaaacca cctaatttt atcaacgtg acgcaatat ttaacacct ggcacgtac acatacgct ggatcaacg ccagttttg cagtttcat ccgaaaaaa ggatttgag actgaagtt tatcaaaag ctgaaaaat gcgtcctca caagatcat tttacagtt tataaaaaa gaggaattt ttaccacga tggcactac aaggacaat cctagaagc cccccgatt ttagtttta gcagaggtt ggatacgga ttagatgat ttaataatt tccgcaaaa caaaatgcg gaaaagaat cattacaaa ttaacaaat cagtcgacc tttggggtt cacggctac gactacact gtcaaagaa atgcgcccc tttttcatg gcaaggggc ccgaaaata aaagtcaat cacaaggtg ccccctttt aacactgtc gatttgttt aacttcttt tgtgcgatt ctggaaatt gcgccaact aaaaataac ggttcgctt gcaaatgtt gaagatatt tttgttgga acaagtggg tattcactg acgactatt ctcacaatc actgcggga ggtatagtc gtttcgttg attttgatt agttgcgcg gctgtttta acgttaatg atgatcaaa agacagcag agtattacc acaactgct gcgttaaat aagcgattt cctcagact tttcaaacg agtattgag gcacaacac ttgctagaa ccagaagat gcgtaa
- >TC004410 RA
gctgacttg tgtcacaat tgtcaagtg tcatgtgtg gcccaaatt ctaaaaaac ttttggaat gattttaaa aatattttt agaatgtct cgtggcaca cttggcata ttccctctc ccagagccc gattctaat cgttgtgtc gtatgacgc cttcaggta tgacttttt cgacacaaa atctgtgcc ccatatgaa cagattgcg aaaattggg gacctactc tgactattt gattaacgt attcccgac caaaacgtt cccaaatca ccattcaat cgccacggg cttgtaccc cgacatcca cggggtggt gggtaacag ttacttcaa taaatcaac aaatagtgt ggttcatat taattatga aatgttcca ctttgatga gaaagttgt cccgatttg gcggcaaaa cgaggatgc gggcaaggg gaggcactc gggggttgt gatgtggcc aggtggttg ctaccccta tcaagggaa acaagtgac gtattgtaa agattacga ccacaattt tgattggtt taagcgcgt tgatttggt gattgggtg gatcaaaga cccgaaaaa gccggctaa tttaataat gctctattt tgatgagcc agacactga tggacacat ttacggcat taactcgga tgtagtcaa taagttgat aaataaatt ggacaatat tacgcttta tttgcaaca acaacttga aaaaaataa tttgacggg taaagtcac agtggtgca tctaagtga ccatgggat gttagatgt gaaaccacc taattttat caacgtgac gcaatattt aacacctgg cacgtacac atacgctgg atcaacgcc agttttgca gtttcatcc gaaaaaagg atttgagac tgaagttta tcaaaagct gaaaaatgc gtcctcaca agatcattt tacagttta taaaaaaga ggaattttt accacgatg gcactacaa ggacaatcc tagaagccc cccgatttt agttttagc agaggttgg atacggatt agatgattt aataatttc cgcaaaaca aaatgcgga aaagaatca ttacaaatt aacaaatca gtcgacctt tggggttca cggctacga ctacactgt caaagaaat gcgcccctt tttcatggc aaggggccc gaaaataaa agtcaatca caaggtgcc cccttttaa cactgtcga tttgtttaa cttcttttg tgcgattct ggaaattgc gccaactaa aaataacgg ttcgcttgc aaatgttga agatatttt tgttggaac aagtgggta ttcactgac gactattct cacaatcac tgcgggagg tatagtcgt ttcgttgat tttgattag ttgcgcggc tgttttaac gttaatgat gatcaaaag acagcagag tattaccac aactgctgc gttaaataa gcgatttcc tcagacttt tcaaacgag tattgaggc acaacactt gctagaacc agaagatgc gtaattgat ttgtgtagt aaatgacca aagattcct agttacgga ggactaaaa ctaagaggc gaaacgata aacaaaatc acttaattt tccctagat aaaaaagga tgttttaaa ttaattttt attcagacg gtcttaagt tcctaataa actactaaa attttcacg aaaaacggg aaaaaacac gtgggtaca agtgaaagt aagcaaaaa attttcaaa ggaatgtca gaagggact acactttta tcaactgta tttcagtta agagttatt tacaactac aattcattt tttctcaaa taatctgat actttctga accacaatt ggaaaaagt gtagtcttt tgttgactg caccctttc tttagtttc tatacaaag tttcacgaa ttactttga ataatttgc actccgtac taaaatttt taaagacac aaaaaagtg taagtttaa aattaaaat gtgctcatt tttactggg aagtttaac tttaaaccg ctgaagttc aattttcta gaactttgt accttataa tagttatct acttgtttg tttgatttg tttgccgtt tacctcgta atattttta gtcctcctt ggtcctatc taattttta taacccatt gtgatttac tgtgtgatg tgtcgaggc gggaccttg acagttttt cgtggtcat aatttaatt tactacagt ttaaaagaa ttgtatatt gtatgattt ataaaaata agataggat aggattcag tttatgctc aattaatta agtttttta accgcccat tagttttta aaaatcaaa atatttctt ttattgtat ttagcatta cttaataac tttaccgac ttaaaaatt tcaaaaaga ggttgaaca tttcaagga gaattaata taatcatta atcagagag tttttgtgg tcacaattt ttatttagt ttttaaaca ttagattgt tacgagtta ttagaatat tgttggttt ctagtcatt taataaaat gatatggaa gaaatgtta aaaagacat tattttaga cagaaaaat aatcaaaaa attgaggct ctttcttgg ttatttttc tcttatttt aatacataa cacattaaa ttaaattag tatgttgcg ttgattttg cggatgaat cattttttc gcttataaa aatgtaaat atctgtatt aaccaaaaa tcaagaaac aaataattc cattattag attattaca tctaataat atggggatg tatacaaga caatatttt ttggatcac ttcgtacat tatattaaa taaaaagga aagaataaa aatttaaaa ctagt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
