Status
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Gene information
General information
TC004405 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 19773545..19780077
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004405
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_06719

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_03747

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004405 RA
      MKDKLTAGA LLRIMKGQE VEEPLVQVL VSKKISSRS 
      AETERYRIW ASDGDYSIT YGILTLPPG KPVEDFSII 
      KLKKFVKSE ISNAKGPQK ILLIIDSEI VTPGHQIGV 
      KIGEPVTLT DEIIASETS STPKVSTSE PPVKQPKSE 
      QSTSLNQSI SETRVLNQI NALTPYHNK WVIKARVTN 
      KSDMRTWSN SRGEGKLFS FDLMDDSGE IRCTAFRDM 
      ADKYFNYLQ VDKVYYISK CQLKAANKQ FNTLKNEYE 
      MTIGNETII EECLTDDGH VPQVKYNFV PISALAEKE 
      VGNLVDVIG ICKEASEVQ TFTSKSTNR ELRKREITL 
      VDQSKTSIA LTLWGSQAD SFDATNNPV VVIKGAKVG 
      EFGGGKNLS TLMSSQIKL NPDIPECHR IKGWYDSEG 
      QYDAMTNLS ERVGGVGSF QTAWMSLKE VQDKGLGHS 
      EKGDYFQVK ATILLVRSE NALYKACPT DDCNKKVVD 
      LENGMYRCE KCCREFPNF KYRLLVSMN IGDFSGNQW 
      VSVFSSEAE KILGKTAQE IGLTMRDDS EAGTAIFQA 
      ANFKQFIFK CRAKMENYN DEQRLKIVV VKVDPVNYE 
      EYNGYLCEQ IEALMV
    CDS sequence
    • >TC004405 RA
      atgaaggat aaactaaca gcgggcgct ttattgcgc 
      attatgaaa ggccaggag gtggaggag cccctagtt 
      caggtgctc gtgagtaag aaaatatcg tcacggtcc 
      gcagagacc gaacgttac agaatatgg gcatctgac 
      ggtgactac tctattact tacggtatt ttgaccctt 
      cctcctggc aaaccagtt gaagatttt tccataata 
      aaattgaaa aagtttgtc aaaagtgaa ataagcaac 
      gctaaaggg ccccaaaaa atacttctc ataatagat 
      tcagaaatt gtcacacct ggtcatcaa attggtgta 
      aaaattggc gaacctgtc actttaact gatgaaatt 
      atcgctagt gaaacttca tcaactcca aaagtttca 
      acctctgaa ccacccgtg aaacaaccc aagtctgaa 
      caatcaacg tccttgaac cagtctatt tctgagact 
      agagttttg aatcaaatt aatgccctc accccttat 
      cacaacaag tgggtgatt aaggcaaga gtcaccaat 
      aagtctgat atgcggact tggtcgaat tcgagaggc 
      gaaggcaag ttgttcagt tttgatctt atggacgac 
      agtggcgaa attagatgt acggccttt agggacatg 
      gctgataaa tacttcaat tatttacag gttgataaa 
      gtttactac ataagcaaa tgccaactc aaggcagct 
      aacaaacaa ttcaacacc ctcaaaaat gagtacgaa 
      atgaccatt gggaacgag actatcatt gaagagtgc 
      ctcactgat gatggccac gtcccacag gtcaaatac 
      aacttcgta ccaatttca gcccttgct gaaaaagag 
      gttggtaat ttagtggac gtgataggc atttgtaaa 
      gaagcgtcc gaagtacag accttcact tcgaaatcg 
      accaaccgc gaactgcgc aagcgcgaa attacgctt 
      gtcgaccaa tcaaaaact tcgattgcg ttgactttg 
      tggggtagt caagccgat tcgttcgat gccacgaat 
      aacccggtt gtcgtcatc aagggggct aaagtgggt 
      gaattcggg ggtggaaaa aatttatca acccttatg 
      agcagtcag attaagttg aatccagat attcccgaa 
      tgccaccga attaagggt tggtacgat tctgaggga 
      cagtacgat gcaatgacg aatttgagc gaaagggtt 
      ggaggagtt gggagtttt cagacggcg tggatgagc 
      ttgaaggag gtgcaagat aaagggttg ggtcattcg 
      gaaaagggc gattatttt caagttaaa gcaacgatt 
      ttattggtg cggtcggaa aatgcgctg tataaagcg 
      tgtccgact gacgattgc aataaaaaa gttgtggat 
      ttggagaat gggatgtac aggtgtgag aaatgttgt 
      cgtgaattt cctaatttc aagtatcgg ttgcttgta 
      tcgatgaac attggcgat tttagcggt aatcagtgg 
      gtgagtgtt ttttcgtca gaagcagag aaaattcta 
      ggaaagact gcgcaagaa attggtctc actatgcga 
      gatgattct gaagctggc actgctatt ttccaggcg 
      gctaatttc aaacagttt atttttaaa tgcagagcc 
      aaaatggaa aattataat gatgaacaa cggttgaaa 
      attgttgtt gttaaagtg gatccagtt aattatgaa 
      gagtataat ggttatttg tgcgaacaa attgaagca 
      ttaatggtg taa
    mRNA sequence
    • >TC004405 RA
      taaccgaag cccgccaaa attgtcact taaccaaac 
      attttttgc gtatgttct gtggtgaaa cattttgta 
      ataattcgt gaaaatgaa ggataaact aacagcggg 
      cgctttatt gcgcattat gaaaggcca ggaggtgga 
      ggagcccct agttcaggt gctcgtgag taagaaaat 
      atcgtcacg gtccgcaga gaccgaacg ttacagaat 
      atgggcatc tgacggtga ctactctat tacttacgg 
      tattttgac ccttcctcc tggcaaacc agttgaaga 
      tttttccat aataaaatt gaaaaagtt tgtcaaaag 
      tgaaataag caacgctaa agggcccca aaaaatact 
      tctcataat agattcaga aattgtcac acctggtca 
      tcaaattgg tgtaaaaat tggcgaacc tgtcacttt 
      aactgatga aattatcgc tagtgaaac ttcatcaac 
      tccaaaagt ttcaacctc tgaaccacc cgtgaaaca 
      acccaagtc tgaacaatc aacgtcctt gaaccagtc 
      tatttctga gactagagt tttgaatca aattaatgc 
      cctcacccc ttatcacaa caagtgggt gattaaggc 
      aagagtcac caataagtc tgatatgcg gacttggtc 
      gaattcgag aggcgaagg caagttgtt cagttttga 
      tcttatgga cgacagtgg cgaaattag atgtacggc 
      ctttaggga catggctga taaatactt caattattt 
      acaggttga taaagttta ctacataag caaatgcca 
      actcaaggc agctaacaa acaattcaa caccctcaa 
      aaatgagta cgaaatgac cattgggaa cgagactat 
      cattgaaga gtgcctcac tgatgatgg ccacgtccc 
      acaggtcaa atacaactt cgtaccaat ttcagccct 
      tgctgaaaa agaggttgg taatttagt ggacgtgat 
      aggcatttg taaagaagc gtccgaagt acagacctt 
      cacttcgaa atcgaccaa ccgcgaact gcgcaagcg 
      cgaaattac gcttgtcga ccaatcaaa aacttcgat 
      tgcgttgac tttgtgggg tagtcaagc cgattcgtt 
      cgatgccac gaataaccc ggttgtcgt catcaaggg 
      ggctaaagt gggtgaatt cgggggtgg aaaaaattt 
      atcaaccct tatgagcag tcagattaa gttgaatcc 
      agatattcc cgaatgcca ccgaattaa gggttggta 
      cgattctga gggacagta cgatgcaat gacgaattt 
      gagcgaaag ggttggagg agttgggag ttttcagac 
      ggcgtggat gagcttgaa ggaggtgca agataaagg 
      gttgggtca ttcggaaaa gggcgatta ttttcaagt 
      taaagcaac gattttatt ggtgcggtc ggaaaatgc 
      gctgtataa agcgtgtcc gactgacga ttgcaataa 
      aaaagttgt ggatttgga gaatgggat gtacaggtg 
      tgagaaatg ttgtcgtga atttcctaa tttcaagta 
      tcggttgct tgtatcgat gaacattgg cgattttag 
      cggtaatca gtgggtgag tgttttttc gtcagaagc 
      agagaaaat tctaggaaa gactgcgca agaaattgg 
      tctcactat gcgagatga ttctgaagc tggcactgc 
      tattttcca ggcggctaa tttcaaaca gtttatttt 
      taaatgcag agccaaaat ggaaaatta taatgatga 
      acaacggtt gaaaattgt tgttgttaa agtggatcc 
      agttaatta tgaagagta taatggtta tttgtgcga 
      acaaattga agcattaat ggtgtaaat ttaatccac 
      tttctacat gatcattgt ttaagtttt tatgtaata 
      ccagtgaat ttagctttt ctttatatt ctttaatta 
      caataagct gattgttga atggcattg cttttttac 
      aaccgtttc gatttttat taatatact gattctgaa 
      tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY