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Gene information
General information
TC004386 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7086353..7087854
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004386
    iBeetle screen iB_03741

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004386 RA
      MATRKCLQL LKIPNRRLF HGNNALLKE GPSFPGAQT 
      TWTEKLSFV TKSSYAPIP VYRVMDRKG SVIDPEGDP 
      QLPRETLLK MYKDMRTLN ILDKILYES QRQGRISFY 
      MTNYGEEAI HIGSAAALD LEDVIYGQY REAGVLVWR 
      GFTAQQFVD QCYGNRDDL GKGRQMPVH YGAKHLNFV 
      TISSPLSTQ MPQAVGAAY ALKGKGRVV VCYYGDGAA 
      SEGDAHAAF NFGATLECP VILFCRNNG YAISTPVKD 
      QYRGDGVAA RGPAFGIHT LRVDGNDVF AVYNATKLA 
      KKYCLEESK PVVVEAMAY RLGHHSTSD DSTAYRSQD 
      EVDQWATTD NPATKMKIY LMKKGLWSE DEEKKFLKE 
      KREEVMTAF TAGEKKPKP NWNECFKDV YKFMPEHIE 
      KQMNDMKKH VDKYKKQYP VDQYET
    CDS sequence
    • >TC004386 RA
      atggcaacg cgaaagtgt ctccaactg ttgaaaatc 
      ccaaatcgg cgcttattc cacgggaat aacgcgtta 
      ttaaaagag ggcccttct ttcccggga gcccagaca 
      acctggacg gagaaattg tctttcgtg acgaagtcc 
      agctatgcc cccatcccc gtgtaccgc gtcatggac 
      cgcaagggc tccgttatc gaccctgaa ggtgacccc 
      cagctcccc agagaaacc ttgctaaaa atgtacaaa 
      gacatgcga acgctcaat atcctcgat aaaatcctg 
      tacgagagt caacgtcag ggccgcatc tcgttctac 
      atgaccaac tacggcgaa gaggccata catattgga 
      agcgcggcc gctttggac ctggaggac gtcatctac 
      ggccaatac cgtgaagcc ggcgtttta gtctggcgg 
      ggattcacc gcccaacaa ttcgtcgat cagtgttac 
      ggcaatcgg gatgatctg ggcaaaggg cgccaaatg 
      ccggtgcat tatggcgcc aaacacttg aattttgtc 
      acgatttcg agccctttg agcacacag atgccccaa 
      gctgtgggg gctgcctac gccctcaag ggcaaaggg 
      cgcgtcgtg gtatgttac tatggtgac ggggcggcg 
      tccgagggg gacgcacac gccgctttt aactttggg 
      gcgacgctg gaatgccca gttattctc ttttgtcgg 
      aataacggg tatgcgatc tcaaccccc gttaaagac 
      cagtaccga ggagacggg gtggcggca agggggccc 
      gcctttggg atccacaca ttaagagta gacgggaat 
      gatgttttt gccgtttac aatgctacc aaacttgcg 
      aaaaaatat tgcctggag gagagtaaa ccagtggtg 
      gttgaggca atggcctat agactgggc caccattca 
      acttcggat gacagtaca gcgtacagg agtcaagac 
      gaggttgac cagtgggcc actactgat aatccagcc 
      acgaaaatg aagatttat ctgatgaag aaaggttta 
      tggagtgaa gatgaagaa aagaagttt ctcaaggag 
      aagagagag gaggtaatg acggctttt acagccggt 
      gagaagaaa ccaaagccc aattggaac gaatgtttt 
      aaggatgtt tacaaattt atgcccgag catatcgag 
      aaacaaatg aacgacatg aagaaacac gtagacaag 
      tacaagaaa cagtacccg gttgatcaa tacgaaacg 
      taa
    mRNA sequence
    • >TC004386 RA
      gaatatttc aacaatcgc cagcgatta cttgataaa 
      ctcgcccgt tattacacc aaaggtcag cccgaaaaa 
      cattaaaca gtgataaaa tggcaacgc gaaagtgtc 
      tccaactgt tgaaaatcc caaatcggc gcttattcc 
      acgggaata acgcgttat taaaagagg gcccttctt 
      tcccgggag cccagacaa cctggacgg agaaattgt 
      ctttcgtga cgaagtcca gctatgccc ccatccccg 
      tgtaccgcg tcatggacc gcaagggct ccgttatcg 
      accctgaag gtgaccccc agctcccca gagaaacct 
      tgctaaaaa tgtacaaag acatgcgaa cgctcaata 
      tcctcgata aaatcctgt acgagagtc aacgtcagg 
      gccgcatct cgttctaca tgaccaact acggcgaag 
      aggccatac atattggaa gcgcggccg ctttggacc 
      tggaggacg tcatctacg gccaatacc gtgaagccg 
      gcgttttag tctggcggg gattcaccg cccaacaat 
      tcgtcgatc agtgttacg gcaatcggg atgatctgg 
      gcaaagggc gccaaatgc cggtgcatt atggcgcca 
      aacacttga attttgtca cgatttcga gccctttga 
      gcacacaga tgccccaag ctgtggggg ctgcctacg 
      ccctcaagg gcaaagggc gcgtcgtgg tatgttact 
      atggtgacg gggcggcgt ccgaggggg acgcacacg 
      ccgctttta actttgggg cgacgctgg aatgcccag 
      ttattctct tttgtcgga ataacgggt atgcgatct 
      caacccccg ttaaagacc agtaccgag gagacgggg 
      tggcggcaa gggggcccg cctttggga tccacacat 
      taagagtag acgggaatg atgtttttg ccgtttaca 
      atgctacca aacttgcga aaaaatatt gcctggagg 
      agagtaaac cagtggtgg ttgaggcaa tggcctata 
      gactgggcc accattcaa cttcggatg acagtacag 
      cgtacagga gtcaagacg aggttgacc agtgggcca 
      ctactgata atccagcca cgaaaatga agatttatc 
      tgatgaaga aaggtttat ggagtgaag atgaagaaa 
      agaagtttc tcaaggaga agagagagg aggtaatga 
      cggctttta cagccggtg agaagaaac caaagccca 
      attggaacg aatgtttta aggatgttt acaaattta 
      tgcccgagc atatcgaga aacaaatga acgacatga 
      agaaacacg tagacaagt acaagaaac agtacccgg 
      ttgatcaat acgaaacgt aatgttagt tttttggaa 
      aaataaaac gctgtaatt ttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY