Status
Please wait ...
Gene information
General information
TC004384 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7053663..7055497
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004384
    iBeetle screen iB_03739

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004384 RA
      MSHWEEFYA THLPPTDFE DNRILLKEF CERHKKAGE 
      RIVLITSGG TTVPLEHNT VRFVDNFSA GTRGAASAE 
      YFLEHGYVV IFMHRLKSL EPFTRHFSG QKFMDMLEL 
      HDQGPNTSI TVKPDSVDV LAPVLASYK HAQEGGRVL 
      YVTFTTVSD YFWLLRAAC ECLAIFETK ALLFLAAAV 
      SDFYVPSNE MPTHKIQSG AGAPTIQLQ LVPKLLAPL 
      VNLWVPHAF VVSFKLETD ESILIVKAR DSLNKYKHK 
      LVIGNMLQT RRQKVVFVT KDTSFEVSL TREQMLNGM 
      EIEDSIVTN VITNHTEFL KTHQY
    CDS sequence
    • >TC004384 RA
      atgtctcat tgggaggag ttttacgcc acgcatttg 
      cccccaact gactttgag gataaccga atcctcctc 
      aaagagttc tgcgagcgt cacaaaaaa gcaggcgaa 
      cgtattgtt ctcataacg tcagggggc acaactgtg 
      ccccttgag cacaacacc gttcgtttc gtcgataat 
      ttcagtgct ggaactcgg ggggctgcc tcagccgag 
      tatttcctc gagcatggt tacgttgtc attttcatg 
      caccgttta aagtcactg gagcccttc acgagacac 
      tttagcggg caaaaattc atggacatg ttagagttg 
      cacgatcag ggccccaat acgagtatc acagtgaaa 
      cccgacagt gtggacgtg ttagccccc gttttggcg 
      agttataag catgcccaa gaagggggc agagtccta 
      tacgtgacg tttacaacc gtctcggat tatttctgg 
      cttttgagg gctgcctgt gaatgcttg gccattttc 
      gagaccaaa gcactttta ttcctagca gctgcagta 
      tccgatttt tacgttcct tcaaacgaa atgccgacg 
      cataaaatc cagtcaggg gcgggcgcc cccacgatt 
      caattgcaa ctagttccg aaattgttg gccccactt 
      gttaattta tgggtgccc catgcgttt gtcgtttcg 
      ttcaaattg gagactgac gaaagtatt ttgatcgta 
      aaagcgaga gattcactc aataaatac aaacataaa 
      cttgtcatc ggaaatatg cttcaaact cggcgccaa 
      aaagtcgtt tttgtcaca aaagacacc agttttgag 
      gtgtctcta acacgcgaa caaatgctc aacggtatg 
      gaaattgaa gactcgatt gttacgaac gtcatcacc 
      aatcacacc gaattcctt aaaacgcat cagtattaa
    mRNA sequence
    • >TC004384 RA
      cgtttgtgc caacagtac agagtccga ctttaccga 
      ccttacgat tttaacgcg aaatgtctc attgggagg 
      agttttacg ccacgcatt tgcccccaa ctgactttg 
      aggataacc gaatcctcc tcaaagagt tctgcgagc 
      gtcacaaaa aagcaggcg aacgtattg ttctcataa 
      cgtcagggg gcacaactg tgccccttg agcacaaca 
      ccgttcgtt tcgtcgata atttcagtg ctggaactc 
      ggggggctg cctcagccg agtatttcc tcgagcatg 
      gttacgttg tcattttca tgcaccgtt taaagtcac 
      tggagccct tcacgagac actttagcg ggcaaaaat 
      tcatggaca tgttagagt tgcacgatc agggcccca 
      atacgagta tcacagtga aacccgaca gtgtggacg 
      tgttagccc ccgttttgg cgagttata agcatgccc 
      aagaagggg gcagagtcc tatacgtga cgtttacaa 
      ccgtctcgg attatttct ggcttttga gggctgcct 
      gtgaatgct tggccattt tcgagacca aagcacttt 
      tattcctag cagctgcag tatccgatt tttacgttc 
      cttcaaacg aaatgccga cgcataaaa tccagtcag 
      gggcgggcg cccccacga ttcaattgc aactagttc 
      cgaaattgt tggccccac ttgttaatt tatgggtgc 
      cccatgcgt ttgtcgttt cgttcaaat tggagactg 
      acgaaagta ttttgatcg taaaagcga gagattcac 
      tcaataaat acaaacata aacttgtca tcggaaata 
      tgcttcaaa ctcggcgcc aaaaagtcg tttttgtca 
      caaaagaca ccagttttg aggtgtctc taacacgcg 
      aacaaatgc tcaacggta tggaaattg aagactcga 
      ttgttacga acgtcatca ccaatcaca ccgaattcc 
      ttaaaacgc atcagtatt aaaaaaatg gggactttc 
      gttaagcga aatcagggt aaataacct taaaacaat 
      ccttgtatt tttaggggc agagggact gaaaatcat 
      gaccattca tttacatat ctttgaacg aacgaagtg 
      ttatattat ttataatta ggagggcaa aaagtcatg 
      ttatgttaa cacaaaagt gtggttata ttatttaaa 
      agttctgat ttttacgtt tcatcgtta atgggctgc 
      cggggcacc taaacaccc ttcttgtaa aattttatt 
      gtttaaaag tgtaatatt aatgcatgt tatgttatg 
      acaaaagtg aggttatgt catttaatt aagattttt 
      tcacgtttg gtcaataat gggggctgc cggggcaca 
      aaaatgtat cgaagtaaa gcgtgtgcg attgctttc 
      tgtgcaatt ttatcgttt taagtgtga taatgcaaa 
      agtgaggtt atgttatct tttgtagaa atggggccc 
      ctgatttgt tagtagtgg ccaaaaaac gtaaatgtt 
      ggtgtaata agtgtaaaa atgtgccag taacgctat 
      cattgtaca gataaatgt tgatatacc tatttttat 
      ttttataca ttcatctac tttggagtt tatgttatt 
      agggggggg gggcacagt ttatgattg tcacaattt 
      tgtaaaaaa gtggctaaa taaatgttt ttattaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY