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Gene information
General information
TC004383 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid77: 76934..92243
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004383
    iBeetle screen iB_03738
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004383 RA
      MKSLVGIMW IVLVLISGC SGNPDAKRL YDDLLSNYN 
      KLVRPVVNV SDALTVKIK LKLSQLIDV NLKNQIMTT 
      NLWVEQSWY DYKLKWDPK EYGGVEMLH VPSDHIWRP 
      DIVLYNNAD GNFEVTLAT KATLNYTGR VEWKPPAIY 
      KSSCEIDVE YFPFDEQTC VMKFGSWTY DGFQVDLRH 
      VDEMKGSNI VDIGIDLTE FYTSVEWDI LEVPAVRNE 
      KFYTCCDEP YLDITFNIT MRRKTLFYT VNLIIPCMG 
      ISFLTVLVF YLPSDSGEK VSLSISILL SLTVFFLLL 
      AEIIPPTSL VVPLLGKFV LFTMILDTF SICVTVVVL 
      NVHFRSPQT HTMAPWVRR VFIHILPRL LVMRRPHYQ 
      FDRKSMTSS HRVMVRTCN GLELRDPGI FTDTEDLVD 
      DPGFFPSAS SRDELTPRE LEAGALNAC RVHGTTPPP 
      GLGLPLPTD LGAGSLEDA LCREANPWY LCPEVHKAI 
      DGVRFIADH TRREEDSTK VKEDWKYVA MVLDRLFLW 
      IFTLAVLVG TAGIILQAP TLYDDRRPI DVRLSEIAS 
      TTAKPHIAS SL
    CDS sequence
    • >TC004383 RA
      atgaagagc ttggtgggg attatgtgg atagtttta 
      gtgctcata tcaggatgt tccggtaat ccagacgcc 
      aaacgactc tacgatgat cttttatca aattataat 
      aaattggtt cggcctgta gtcaacgtt tcggacgct 
      ctaactgtc aaaatcaaa ctcaaacta tcgcaactc 
      atcgatgtt aacctcaag aaccaaata atgacaacg 
      aatctgtgg gtcgaacag tcatggtac gactacaag 
      ctcaaatgg gaccccaaa gagtacggc ggtgtggag 
      atgctccat gtgccctct gaccacatc tggcgtccg 
      gacatcgtc ctctacaac aatgccgat ggtaatttc 
      gaggtgact ttagccacc aaagccacc ctcaattac 
      accggaagg gtggagtgg aaacctcca gcgatttac 
      aagtcttcc tgcgagata gacgtcgag tatttcccc 
      ttcgacgag caaacgtgt gtcatgaaa tttggatcg 
      tggacttac gacggcttc caggtggat cttcgtcat 
      gtggacgag atgaagggc tccaatatt gtcgatatc 
      ggcatcgac ttgaccgaa ttctacact tcggtcgaa 
      tgggacatc ttggaggtc ccagccgtc aggaacgaa 
      aagttttat acgtgttgt gatgaacct tatcttgat 
      atcacattc aacattacg atgagacga aaaacttta 
      ttttatacc gtcaatctc atcattccg tgtatgggc 
      atttcgttt ctcaccgtt ttagtgttc tatctgcct 
      tccgatagt ggtgaaaaa gtaagtttg tccatctcg 
      atcctacta tcactgaca gtgttcttc ctactacta 
      gccgaaatt atcccaccg acgtcattg gtggtccca 
      ttgttgggc aaatttgta ctttttacc atgattttg 
      gacactttc agcatatgc gtgacagtg gtagtcctc 
      aacgtccat ttccggtct cctcagacc cataccatg 
      gccccatgg gtgcgtcgt gtcttcatc cacatcctc 
      ccacgtctc ttggtgatg agacgaccc cattaccaa 
      ttcgaccgg aaaagcatg acgtccagt catcgtgtc 
      atggtacga acctgcaat gggttggag cttcgcgat 
      cctgggatt tttacagac acggaggat ttggtcgat 
      gatcccggt tttttccca agcgctagt tccagggat 
      gagctaaca cccagggag ttggaggct ggggccttg 
      aatgcgtgt cgggtccat ggtactacc ccaccgcca 
      gggctcggg ttgccttta ccaacggat ctgggcgct 
      gggtcgctt gaggatgcg ctttgtcgg gaggccaac 
      ccatggtac ttgtgtcca gaggtgcat aaagccatt 
      gatggggtc aggtttatt gcggaccat accagacgt 
      gaggaggat tcgactaag gttaaggaa gactggaag 
      tacgttgca atggtacta gaccgactc tttctttgg 
      attttcaca cttgcggta cttgttggt accgctggt 
      attatttta caagctccg actttgtac gatgatcgt 
      agacccatc gatgttaga ctttcggag attgcatcg 
      accactgct aaaccccat attgccagt tctttataa
    mRNA sequence
    • >TC004383 RA
      tgcctcaac cacagatag gttcatccg agtttgcaa 
      gacacgatc actggtttt cgatgatgg tgttgcaga 
      tttgtctga actgaacgg atgtgaacg agattttgg 
      aaactgaaa ggtccacca tgtggacat gactcggga 
      tgaagagct tggtgggga ttatgtgga tagttttag 
      tgctcatat caggatgtt ccggtaatc cagacgcca 
      aacgactct acgatgatc ttttatcaa attataata 
      aattggttc ggcctgtag tcaacgttt cggacgctc 
      taactgtca aaatcaaac tcaaactat cgcaactca 
      tcgatgtta acctcaaga accaaataa tgacaacga 
      atctgtggg tcgaacagt catggtacg actacaagc 
      tcaaatggg accccaaag agtacggcg gtgtggaga 
      tgctccatg tgccctctg accacatct ggcgtccgg 
      acatcgtcc tctacaaca atgccgatg gtaatttcg 
      aggtgactt tagccacca aagccaccc tcaattaca 
      ccggaaggg tggagtgga aacctccag cgatttaca 
      agtcttcct gcgagatag acgtcgagt atttcccct 
      tcgacgagc aaacgtgtg tcatgaaat ttggatcgt 
      ggacttacg acggcttcc aggtggatc ttcgtcatg 
      tggacgaga tgaagggct ccaatattg tcgatatcg 
      gcatcgact tgaccgaat tctacactt cggtcgaat 
      gggacatct tggaggtcc cagccgtca ggaacgaaa 
      agttttata cgtgttgtg atgaacctt atcttgata 
      tcacattca acattacga tgagacgaa aaactttat 
      tttataccg tcaatctca tcattccgt gtatgggca 
      tttcgtttc tcaccgttt tagtgttct atctgcctt 
      ccgatagtg gtgaaaaag taagtttgt ccatctcga 
      tcctactat cactgacag tgttcttcc tactactag 
      ccgaaatta tcccaccga cgtcattgg tggtcccat 
      tgttgggca aatttgtac tttttacca tgattttgg 
      acactttca gcatatgcg tgacagtgg tagtcctca 
      acgtccatt tccggtctc ctcagaccc ataccatgg 
      ccccatggg tgcgtcgtg tcttcatcc acatcctcc 
      cacgtctct tggtgatga gacgacccc attaccaat 
      tcgaccgga aaagcatga cgtccagtc atcgtgtca 
      tggtacgaa cctgcaatg ggttggagc ttcgcgatc 
      ctgggattt ttacagaca cggaggatt tggtcgatg 
      atcccggtt ttttcccaa gcgctagtt ccagggatg 
      agctaacac ccagggagt tggaggctg gggccttga 
      atgcgtgtc gggtccatg gtactaccc caccgccag 
      ggctcgggt tgcctttac caacggatc tgggcgctg 
      ggtcgcttg aggatgcgc tttgtcggg aggccaacc 
      catggtact tgtgtccag aggtgcata aagccattg 
      atggggtca ggtttattg cggaccata ccagacgtg 
      aggaggatt cgactaagg ttaaggaag actggaagt 
      acgttgcaa tggtactag accgactct ttctttgga 
      ttttcacac ttgcggtac ttgttggta ccgctggta 
      ttattttac aagctccga ctttgtacg atgatcgta 
      gacccatcg atgttagac tttcggaga ttgcatcga 
      ccactgcta aaccccata ttgccagtt ctttataag 
      gaatttgct gaaaatttg gttcctggg ccaaaaaca 
      gacgcaaaa ttgtcaaaa acacttttt gtttttgac 
      taatggcaa aaaaaacac aaaaacaac aaaacagtt 
      cttagtttt ttgtgacat gtttgtcaa aaacaaaaa 
      aaaccgtag tcaaaaaca caaaaaaaa caagtctct 
      ttatcattt attacgcca catatttgt caaaaccag 
      aagaaagtg gactggcat tgtcaaata cacaccaaa 
      ttgcgacac cgatttgtc aaatacaaa aaaattgaa 
      ctgatttag tcaaggcct ggggttctt tttttcggc 
      tcttttcgg ggtcgagcc acgtagaaa aaagaagaa 
      gaaaaaaat ttaatgtga gttttcgga cggatgctc 
      aaattttca actgtccaa aaaaaaata gtcgaaaag 
      tttagtatt ttatggccg ataggaatt gtacctttc 
      tagtcatct cccattttc tctatcact tataattag 
      taaatcgga cggcagaaa tccctaact taattattt 
      actcgttat aagaccgaa agtgggtga ttagatttg 
      ccttctgca cgtttttat tattacgtt taatataaa 
      aaaaacttc aaattttaa tgatacctt aaaaccgtg 
      tttttaaac gctgccgaa tgattataa agaaattat 
      attaacgca tcgttattt taaaaaaac atgtaacta 
      tgatgatgt aaacgattt ctatacata tttatgctg 
      taactaacg attttaata aattaaatt tttgata