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Gene information
General information
TC004381 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid77: 59844..66202
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004381
    iBeetle screen iB_03737
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004381 RA
      MPHFWDYFS KSPRKQRNL QDGLFQIAS QTCNLLVQV 
      NNTNNISYG NGSTNIHLN DISIPITRP EIPTPQNDT 
      DREQLLVLI PRKMSKIKG PGLGKRLST VLEDQGGNH 
      DDDNHLWGH GYNQDCDGS PVRNRWGQK NKWCRPSCI 
      PISLICILI FLVVLLPLL DHATERALE DLNRAVVEP 
      CNTCSVSLV ESIPEGLTF NNSIVHTST FETWFNLIQ 
      SAESSIEIA SFYWTLRQS EVYPDPSSV KGEIIFQAL 
      LKAGTDRKI SIKIAQNSP TQDNPNVDT ELLVKRKAA 
      QVRSLNFAK LLGGGVLHT KFWIIDRKH VYIGSANMD 
      WRALTQVKE MGLVINNCS CLAEDLGKV FDVYWKLGE 
      DGARIPDQW PSNLSTNIN VNTPMNLTL SNETFQTYI 
      SSSPLSFNP SGRTNDIDA LLSVIKHAE KFIYIAVMD 
      YFPLTIYTP KIKFWPKID DALKTAAIE HHVKVKMLI 
      SWWNHSRSS EDNFLKSLA SINLAYPRV SIEVRRFIV 
      PSNKEQAKI PFARVNHNK YMVTDNTAF IGTSNWSGD 
      YFIDTAGVS FVLHDPVFD RNGTEATLR SELQAVFER 
      DWNSNYSVP LKL
    • >TC004381 RB
      MKVWLPSWH PLEKTITVL EDQGGNHDD DNHLWGHGY 
      NQDCDGSPV RNRWGQKNK WCRPSCIPI SLICILIFL 
      VVLLPLLDH ATERALEDL NRAVVEPCN TCSVSLVES 
      IPEGLTFNN SIVHTSTFE TWFNLIQSA ESSIEIASF 
      YWTLRQSEV YPDPSSVKG EIIFQALLK AGTDRKISI 
      KIAQNSPTQ DNPNVDTEL LVKRKAAQV RSLNFAKLL 
      GGGVLHTKF WIIDRKHVY IGSANMDWR ALTQVKEMG 
      LVINNCSCL AEDLGKVFD VYWKLGEDG ARIPDQWPS 
      NLSTNINVN TPMNLTLSN ETFQTYISS SPLSFNPSG 
      RTNDIDALL SVIKHAEKF IYIAVMDYF PLTIYTPKI 
      KFWPKIDDA LKTAAIEHH VKVKMLISW WNHSRSSED 
      NFLKSLASI NLAYPRVSI EVRRFIVPS NKEQAKIPF 
      ARVNHNKYM VTDNTAFIG TSNWSGDYF IDTAGVSFV 
      LHDPVFDRN GTEATLRSE LQAVFERDW NSNYSVPLK 
      L
    CDS sequence
    • >TC004381 RA
      atgccgcac ttttgggac tatttctca aaaagtccg 
      cgcaaacaa cgcaatttg caagacggt ctgtttcaa 
      atcgcgtcg caaacatgc aatttgctc gttcaagtg 
      aacaacacc aacaacata agttacgga aatggcagt 
      acaaatatt catctcaac gatatctcc atccctatc 
      acgcgtccc gaaattccc acgccgcaa aatgacacc 
      gacagggag cagctgctg gtgctcata cctcggaaa 
      atgtccaaa atcaaagga ccaggcttg ggaaagcgc 
      ctatcgact gtgttagaa gaccagggg ggcaaccac 
      gacgacgac aatcaccta tggggccac ggctacaac 
      caggactgt gacggcagc cccgtccga aacagatgg 
      ggccagaaa aacaagtgg tgtcggcct tcctgtatc 
      cccatttcg ctaatttgc attctgatc ttcctcgta 
      gttctttta ccgctctta gaccacgcc actgaacgg 
      gctttggag gacttgaac agggctgta gtggagccc 
      tgtaacact tgcagtgtg tctttagtc gagagcatt 
      cccgaaggt ctaacgttc aacaacagc attgttcac 
      acttcgacg ttcgaaacg tggttcaat ttaatccag 
      tcggctgag agttccatc gaaatagcg tcgttttac 
      tggactttg aggcagtca gaagtgtac cctgacccg 
      tcctcggtc aagggcgag attattttc caagcgttg 
      ttgaaggcg ggaaccgat cggaaaatc tccataaaa 
      attgcacaa aattcgccg acgcaagac aatccaaat 
      gtggacact gagcttcta gttaagcgc aaggcggct 
      caagtgagg agtttaaat tttgcgaaa ttgctaggt 
      gggggcgtt ttgcatacg aagttttgg attattgat 
      aggaaacat gtgtacatt gggagtgct aacatggac 
      tggagggct ctgactcaa gtcaaagaa atggggttg 
      gtgattaat aattgtagt tgtctggct gaggatctg 
      gggaaggtt tttgatgtg tattggaaa ttgggcgaa 
      gacggagcg agaattccc gatcaatgg ccgtccaat 
      ttatccaca aatattaat gtgaatact cctatgaac 
      cttactcta agtaatgaa acgtttcaa acgtacata 
      tcgagttca ccgttgtct ttcaaccct tctggtcgc 
      accaacgac atcgacgcc ctactcagc gtcatcaag 
      cacgccgaa aaattcatt tacatcgcc gtaatggac 
      tactttcct ctaaccatc tacacgccg aaaataaaa 
      ttttggcca aaaatcgac gatgccttg aaaacggcc 
      gccatcgaa caccatgtc aaagtaaaa atgttgatt 
      agttggtgg aatcattcg cgttcatct gaagacaat 
      ttcttaaaa tcactcgct tccattaac ttggcatat 
      cctagagta tcgatcgaa gtgcgacga tttattgtg 
      ccttcaaat aaagaacaa gcaaaaata cctttcgcg 
      cgagttaat cacaacaaa tacatggtc actgataat 
      accgctttt atagggact tctaattgg tcgggagat 
      tattttatc gatacagcg ggcgtttca tttgttttg 
      catgacccg gtgtttgac aggaacggc actgaggcc 
      actctaagg agcgaattg caagccgtg tttgaaaga 
      gattggaac tctaattac tcagttccg ttgaaatta 
      taa
    • >TC004381 RB
      atgaaagtg tggttaccg tcatggcac cctttggaa 
      aaaacaatc actgtgtta gaagaccag gggggcaac 
      cacgacgac gacaatcac ctatggggc cacggctac 
      aaccaggac tgtgacggc agccccgtc cgaaacaga 
      tggggccag aaaaacaag tggtgtcgg ccttcctgt 
      atccccatt tcgctaatt tgcattctg atcttcctc 
      gtagttctt ttaccgctc ttagaccac gccactgaa 
      cgggctttg gaggacttg aacagggct gtagtggag 
      ccctgtaac acttgcagt gtgtcttta gtcgagagc 
      attcccgaa ggtctaacg ttcaacaac agcattgtt 
      cacacttcg acgttcgaa acgtggttc aatttaatc 
      cagtcggct gagagttcc atcgaaata gcgtcgttt 
      tactggact ttgaggcag tcagaagtg taccctgac 
      ccgtcctcg gtcaagggc gagattatt ttccaagcg 
      ttgttgaag gcgggaacc gatcggaaa atctccata 
      aaaattgca caaaattcg ccgacgcaa gacaatcca 
      aatgtggac actgagctt ctagttaag cgcaaggcg 
      gctcaagtg aggagttta aattttgcg aaattgcta 
      ggtgggggc gttttgcat acgaagttt tggattatt 
      gataggaaa catgtgtac attgggagt gctaacatg 
      gactggagg gctctgact caagtcaaa gaaatgggg 
      ttggtgatt aataattgt agttgtctg gctgaggat 
      ctggggaag gtttttgat gtgtattgg aaattgggc 
      gaagacgga gcgagaatt cccgatcaa tggccgtcc 
      aatttatcc acaaatatt aatgtgaat actcctatg 
      aaccttact ctaagtaat gaaacgttt caaacgtac 
      atatcgagt tcaccgttg tctttcaac ccttctggt 
      cgcaccaac gacatcgac gccctactc agcgtcatc 
      aagcacgcc gaaaaattc atttacatc gccgtaatg 
      gactacttt cctctaacc atctacacg ccgaaaata 
      aaattttgg ccaaaaatc gacgatgcc ttgaaaacg 
      gccgccatc gaacaccat gtcaaagta aaaatgttg 
      attagttgg tggaatcat tcgcgttca tctgaagac 
      aatttctta aaatcactc gcttccatt aacttggca 
      tatcctaga gtatcgatc gaagtgcga cgatttatt 
      gtgccttca aataaagaa caagcaaaa atacctttc 
      gcgcgagtt aatcacaac aaatacatg gtcactgat 
      aataccgct tttataggg acttctaat tggtcggga 
      gattatttt atcgataca gcgggcgtt tcatttgtt 
      ttgcatgac ccggtgttt gacaggaac ggcactgag 
      gccactcta aggagcgaa ttgcaagcc gtgtttgaa 
      agagattgg aactctaat tactcagtt ccgttgaaa 
      ttataa
    mRNA sequence
    • >TC004381 RA
      caagtccac ttagcacac gtgcaaatg ttcctccaa 
      ccgggctaa aaaaaaatg ccgcacttt tgggactat 
      ttctcaaaa agtccgcgc aaacaacgc aatttgcaa 
      gacggtctg tttcaaatc gcgtcgcaa acatgcaat 
      ttgctcgtt caagtgaac aacaccaac aacataagt 
      tacggaaat ggcagtaca aatattcat ctcaacgat 
      atctccatc cctatcacg cgtcccgaa attcccacg 
      ccgcaaaat gacaccgac agggagcag ctgctggtg 
      ctcatacct cggaaaatg tccaaaatc aaaggacca 
      ggcttggga aagcgccta tcgactgtg ttagaagac 
      caggggggc aaccacgac gacgacaat cacctatgg 
      ggccacggc tacaaccag gactgtgac ggcagcccc 
      gtccgaaac agatggggc cagaaaaac aagtggtgt 
      cggccttcc tgtatcccc atttcgcta atttgcatt 
      ctgatcttc ctcgtagtt cttttaccg ctcttagac 
      cacgccact gaacgggct ttggaggac ttgaacagg 
      gctgtagtg gagccctgt aacacttgc agtgtgtct 
      ttagtcgag agcattccc gaaggtcta acgttcaac 
      aacagcatt gttcacact tcgacgttc gaaacgtgg 
      ttcaattta atccagtcg gctgagagt tccatcgaa 
      atagcgtcg ttttactgg actttgagg cagtcagaa 
      gtgtaccct gacccgtcc tcggtcaag ggcgagatt 
      attttccaa gcgttgttg aaggcggga accgatcgg 
      aaaatctcc ataaaaatt gcacaaaat tcgccgacg 
      caagacaat ccaaatgtg gacactgag cttctagtt 
      aagcgcaag gcggctcaa gtgaggagt ttaaatttt 
      gcgaaattg ctaggtggg ggcgttttg catacgaag 
      ttttggatt attgatagg aaacatgtg tacattggg 
      agtgctaac atggactgg agggctctg actcaagtc 
      aaagaaatg gggttggtg attaataat tgtagttgt 
      ctggctgag gatctgggg aaggttttt gatgtgtat 
      tggaaattg ggcgaagac ggagcgaga attcccgat 
      caatggccg tccaattta tccacaaat attaatgtg 
      aatactcct atgaacctt actctaagt aatgaaacg 
      tttcaaacg tacatatcg agttcaccg ttgtctttc 
      aacccttct ggtcgcacc aacgacatc gacgcccta 
      ctcagcgtc atcaagcac gccgaaaaa ttcatttac 
      atcgccgta atggactac tttcctcta accatctac 
      acgccgaaa ataaaattt tggccaaaa atcgacgat 
      gccttgaaa acggccgcc atcgaacac catgtcaaa 
      gtaaaaatg ttgattagt tggtggaat cattcgcgt 
      tcatctgaa gacaatttc ttaaaatca ctcgcttcc 
      attaacttg gcatatcct agagtatcg atcgaagtg 
      cgacgattt attgtgcct tcaaataaa gaacaagca 
      aaaatacct ttcgcgcga gttaatcac aacaaatac 
      atggtcact gataatacc gcttttata gggacttct 
      aattggtcg ggagattat tttatcgat acagcgggc 
      gtttcattt gttttgcat gacccggtg tttgacagg 
      aacggcact gaggccact ctaaggagc gaattgcaa 
      gccgtgttt gaaagagat tggaactct aattactca 
      gttccgttg aaattataa ttatatttt tttcattgg 
      tttactaag actcaatat tcatttgag acaataagt 
      cagcgtatt gtttttttt tgaaaagtg acaagtgac 
      attaagtgc ctcactaaa ttgtatttt ttaaacttt 
      tgctcataa ataagtcaa tgtattccg tcaaaataa 
      cacaatgtt tgaatcttc gtgaaaata aatatttgg 
      gaactaa
    • >TC004381 RB
      caagtccac ttagcacac gtgcaaatg ttcctccaa 
      ccgggctaa aaaaaaatg ccgcacttt tgggactat 
      ttctcaaaa agtccgcgc aaacaacgc aatttgcaa 
      gacggtctg tttcaaatc gcgtcgcaa acatgcaat 
      ttgctcgtt caagtgaac aacaccaac aacataagt 
      tacggaaat ggcagtaca aatattcat ctcaacgat 
      atctccatc cctatcacg cgtcccgaa attcccacg 
      ccgcaaaat gacaccgac agggagcag ctgctggtg 
      ctcatacct cggaaaatg tccaaaatc aaaggacca 
      ggcttggga aagcgccta tcgttatta taattggct 
      ttgatttta ttttcatat aaatataaa tttcccaaa 
      aaatgctga ctctaaccc aactttttg ccaaccttg 
      ccataattg gcttagctt atcagttca cattttaaa 
      tcaaatcag atatggtca tttattaat tgaattaaa 
      caatgaaag tgtggttac cgtcatggc accctttgg 
      aaaaaacaa tcactgtgt tagaagacc aggggggca 
      accacgacg acgacaatc acctatggg gccacggct 
      acaaccagg actgtgacg gcagccccg tccgaaaca 
      gatggggcc agaaaaaca agtggtgtc ggccttcct 
      gtatcccca tttcgctaa tttgcattc tgatcttcc 
      tcgtagttc ttttaccgc tcttagacc acgccactg 
      aacgggctt tggaggact tgaacaggg ctgtagtgg 
      agccctgta acacttgca gtgtgtctt tagtcgaga 
      gcattcccg aaggtctaa cgttcaaca acagcattg 
      ttcacactt cgacgttcg aaacgtggt tcaatttaa 
      tccagtcgg ctgagagtt ccatcgaaa tagcgtcgt 
      tttactgga ctttgaggc agtcagaag tgtaccctg 
      acccgtcct cggtcaagg gcgagatta ttttccaag 
      cgttgttga aggcgggaa ccgatcgga aaatctcca 
      taaaaattg cacaaaatt cgccgacgc aagacaatc 
      caaatgtgg acactgagc ttctagtta agcgcaagg 
      cggctcaag tgaggagtt taaattttg cgaaattgc 
      taggtgggg gcgttttgc atacgaagt tttggatta 
      ttgatagga aacatgtgt acattggga gtgctaaca 
      tggactgga gggctctga ctcaagtca aagaaatgg 
      ggttggtga ttaataatt gtagttgtc tggctgagg 
      atctgggga aggtttttg atgtgtatt ggaaattgg 
      gcgaagacg gagcgagaa ttcccgatc aatggccgt 
      ccaatttat ccacaaata ttaatgtga atactccta 
      tgaacctta ctctaagta atgaaacgt ttcaaacgt 
      acatatcga gttcaccgt tgtctttca acccttctg 
      gtcgcacca acgacatcg acgccctac tcagcgtca 
      tcaagcacg ccgaaaaat tcatttaca tcgccgtaa 
      tggactact ttcctctaa ccatctaca cgccgaaaa 
      taaaatttt ggccaaaaa tcgacgatg ccttgaaaa 
      cggccgcca tcgaacacc atgtcaaag taaaaatgt 
      tgattagtt ggtggaatc attcgcgtt catctgaag 
      acaatttct taaaatcac tcgcttcca ttaacttgg 
      catatccta gagtatcga tcgaagtgc gacgattta 
      ttgtgcctt caaataaag aacaagcaa aaatacctt 
      tcgcgcgag ttaatcaca acaaataca tggtcactg 
      ataataccg cttttatag ggacttcta attggtcgg 
      gagattatt ttatcgata cagcgggcg tttcatttg 
      ttttgcatg acccggtgt ttgacagga acggcactg 
      aggccactc taaggagcg aattgcaag ccgtgtttg 
      aaagagatt ggaactcta attactcag ttccgttga 
      aattataat tatattttt ttcattggt ttactaaga 
      ctcaatatt catttgaga caataagtc agcgtattg 
      ttttttttt gaaaagtga caagtgaca ttaagtgcc 
      tcactaaat tgtattttt taaactttt gctcataaa 
      taagtcaat gtattccgt caaaataac acaatgttt 
      gaatcttcg tgaaaataa atatttggg aactaa