Status
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Gene information
General information
TC031454
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC004363,TC004362
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7101697..7105531
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031454
    iBeetle screen iB_03734

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031454 RA
      MPRRRYTPE TDPREKIKD YFRKFIAFM CSQVGVGAL 
      VVCYTLIGA VGFSRLEST FNDTSVTRV ASIRGNYTR 
      LLWLVARKT NVFNQTEFF IDTNEKLKN FQNEMVLVI 
      KKGYNGHDG GKMWTFPAA LMFALSVIT MIGYGNLVP 
      RTGWGKFAT VVYAVFGIP LFVLYFLNV GEILAGCFK 
      WVYTKLYEC STKRGEEKV HKRIVVPTT ACLWVMGGY 
      ILTGAIMFA EWEHWTYLD SAYFCVTSL CKLGLGDFV 
      PGTASQNGN ESKLVINFI YILVGMGLV AMCFNLMRE 
      EVRVKVEEF REDFRQCLE DTRVRIEEW YCIGMRYLN 
      VNGYENRTN DVIYIRPLQ NGNKTAVIY FGGDIQDFT 
      ENMQSHRDN KNYLDWSLD NTSQILQNA FPASHVILI 
      RPARMEYKT FSCFENFVP CANCGVPQH TPMHHAVEH 
      LENLIGNLE KLSQDCLSD LDLVLIGFS KGCVVLNQF 
      LYEFHTLGE KTQFVEKIK TMFWLDGGH SGGKNTWVT 
      SRPILETLA KFGIQVRIH VSPYQIGDE RRPWIKKEE 
      RIFYNTLFG LNVQVARLV HSPDLPPTI YQHFAVLNE 
      FNRK
    CDS sequence
    • >TC031454 RA
      atgcctcgc cgtcgttac acacccgaa acggaccct 
      cgcgaaaaa atcaaagac tattttcgc aaatttatc 
      gcatttatg tgttcgcaa gtgggcgtt ggggccctt 
      gttgtgtgt tacactcta ataggggca gtcggtttc 
      agccgactc gagtcgaca tttaacgac acttccgtc 
      actcgagtc gcttcaatt cgggggaat tacacgcgt 
      ttgttgtgg ttagtggcg cgcaaaact aacgttttc 
      aaccaaact gaatttttc attgacaca aacgagaag 
      ttgaagaat tttcaaaac gaaatggtt ctcgtgatt 
      aaaaagggg tacaatgga cacgacggg ggcaaaatg 
      tggacgttc ccagccgcc ttgatgttc gccttaagt 
      gtcattaca atgatcggg tacgggaat ttagtgccg 
      cgcaccggt tggggaaaa ttcgcaacg gtggtttac 
      gccgtgttt gggattccc ctatttgtg ctgtatttc 
      ctcaacgtg ggggaaatc ctggctggg tgtttcaag 
      tgggtttat acgaagttg tacgaatgt agtacgaaa 
      cggggggag gagaaggtg cataagagg attgtggtc 
      cccactaca gcatgtctt tgggtcatg gggggctat 
      attctcacg ggggctatc atgtttgct gaatgggag 
      cattggacc tacttagac agtgcctat ttttgcgtt 
      accagtctt tgtaaactg ggtttaggg gactttgtc 
      cctggaact gcctcacaa aatggaaac gaatcgaaa 
      cttgtgatt aattttatt tatattttg gtggggatg 
      gggttagtg gccatgtgt ttcaatttg atgagggaa 
      gaagtgagg gtgaaagtt gaggagttt agggaggat 
      tttagacag tgtttggag gatacgaga gtgaggatt 
      gaggagtgg tattgtatt ggtatgagg tatctcaat 
      gtgaatgga tacgaaaat agaacaaac gacgtcatt 
      tacatccga ccgcttcaa aatgggaac aaaaccgcc 
      gtgatttac ttcggaggt gatattcag gactttacc 
      gagaacatg caaagccac cgagataat aaaaactac 
      ctcgactgg agtttagat aacacgagt caaatccta 
      caaaatgca ttccccgcc tcacacgtg attttaatt 
      cgacctgcg agaatggag tataagacg ttcagttgt 
      ttcgaaaac ttcgtccct tgtgccaac tgtggggtt 
      ccccaacac acaccaatg caccacgct gttgagcat 
      cttgaaaac ttgatcgga aatttggaa aaattgtcc 
      caggactgt ttaagcgac cttgatttg gttttaatc 
      ggttttagt aaaggttgc gtggttttg aaccaattt 
      ctttacgag tttcacaca ttgggagaa aaaacacag 
      tttgtggag aaaatcaaa actatgttt tggttggat 
      gggggacac agtgggggg aagaacact tgggtcacc 
      tccaggccg atcttggag actttggca aaatttggt 
      attcaagtg agaattcac gtaagtccg tatcagatt 
      ggggacgaa cgcaggccc tggattaaa aaggaagaa 
      cgcatattt tacaacact ttgtttggg ttaaacgta 
      caagttgcc cggctggtg cattcacca gatttgcct 
      ccaacaatt tatcaacat tttgcggtt ttgaacgaa 
      tttaatcga aaataa
    mRNA sequence
    • >TC031454 RA
      gttttttcg tgtaataac ccactcaaa aggctttag 
      aaccgctct gaagtcgct aatcgagcc ctcagtccg 
      gcgacgaag cctcacaac aggtaagtg tgaacttgt 
      gtataactg gttgactac caggcaaat aattccggg 
      ggctatttt tagccgctt tcctcagtc tgccaccat 
      gcctcgccg tcgttacac acccgaaac ggaccctcg 
      cgaaaaaat caaagacta ttttcgcaa atttatcgc 
      atttatgtg ttcgcaagt gggcgttgg ggcccttgt 
      tgtgtgtta cactctaat aggggcagt cggtttcag 
      ccgactcga gtcgacatt taacgacac ttccgtcac 
      tcgagtcgc ttcaattcg ggggaatta cacgcgttt 
      gttgtggtt agtggcgcg caaaactaa cgttttcaa 
      ccaaactga atttttcat tgacacaaa cgagaagtt 
      gaagaattt tcaaaacga aatggttct cgtgattaa 
      aaaggggta caatggaca cgacggggg caaaatgtg 
      gacgttccc agccgcctt gatgttcgc cttaagtgt 
      cattacaat gatcgggta cgggaattt agtgccgcg 
      caccggttg gggaaaatt cgcaacggt ggtttacgc 
      cgtgtttgg gattcccct atttgtgct gtatttcct 
      caacgtggg ggaaatcct ggctgggtg tttcaagtg 
      ggtttatac gaagttgta cgaatgtag tacgaaacg 
      gggggagga gaaggtgca taagaggat tgtggtccc 
      cactacagc atgtctttg ggtcatggg gggctatat 
      tctcacggg ggctatcat gtttgctga atgggagca 
      ttggaccta cttagacag tgcctattt ttgcgttac 
      cagtctttg taaactggg tttagggga ctttgtccc 
      tggaactgc ctcacaaaa tggaaacga atcgaaact 
      tgtgattaa ttttattta tattttggt ggggatggg 
      gttagtggc catgtgttt caatttgat gagggaaga 
      agtgagggt gaaagttga ggagtttag ggaggattt 
      tagacagtg tttggagga tacgagagt gaggattga 
      ggagtggta ttgtattgg tatgaggta tctcaatgt 
      gaatggata cgaaaatag aacaaacga cgtcattta 
      catccgacc gcttcaaaa tgggaacaa aaccgccgt 
      gatttactt cggaggtga tattcagga ctttaccga 
      gaacatgca aagccaccg agataataa aaactacct 
      cgactggag tttagataa cacgagtca aatcctaca 
      aaatgcatt ccccgcctc acacgtgat tttaattcg 
      acctgcgag aatggagta taagacgtt cagttgttt 
      cgaaaactt cgtcccttg tgccaactg tggggttcc 
      ccaacacac accaatgca ccacgctgt tgagcatct 
      tgaaaactt gatcggaaa tttggaaaa attgtccca 
      ggactgttt aagcgacct tgatttggt tttaatcgg 
      ttttagtaa aggttgcgt ggttttgaa ccaatttct 
      ttacgagtt tcacacatt gggagaaaa aacacagtt 
      tgtggagaa aatcaaaac tatgttttg gttggatgg 
      gggacacag tggggggaa gaacacttg ggtcacctc 
      caggccgat cttggagac tttggcaaa atttggtat 
      tcaagtgag aattcacgt aagtccgta tcagattgg 
      ggacgaacg caggccctg gattaaaaa ggaagaacg 
      catatttta caacacttt gtttgggtt aaacgtaca 
      agttgcccg gctggtgca ttcaccaga tttgcctcc 
      aacaattta tcaacattt tgcggtttt gaacgaatt 
      taatcgaaa ataaagaat taaaaaaaa actagtgat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY