Status
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Gene information
General information
TC004357 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7159160..7164331
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004357
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03733

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004357 RA
      MDNPEVCPI TADLYFLLH KFLSGGPLK KTLKALHEE 
      LTENKLLPQ RTDWLGKLH DRTLEDLEH QYPNVRPDY 
      LLQLCCRAG TQKVTQVEK IATLFSLKK HAVNSSNVE 
      YNFKQLCNY VQRRHLLPL CNRVHISSN LVNILRGRE 
      ISGPISRRW VVAPKLYSG LQFQRRTLG HLSAVYCLL 
      FDYSGRYII TGADDLLIK LWSSVTGRL IATFRGASS 
      EITDIAINL ENTLLAAGS IDRILRIWD LQTGIPVAV 
      LPGHTGMIT SVNFCPSPS WNFQYLITT STDGSVAFW 
      AYTHEPGGK VNFHAKPTM YQEKMRPGQ AQMICSSFS 
      PGGNFLATG SADHHVRVY YMKGDEGPH RILETEAHN 
      DRVDSIQWA HEGLKFLSG SKDGTAIIW SFESQQWKT 
      TYLHMTAAS NTVKPTSTK KMAEEARKL KVTMVTWDR 
      SDAWAITAV SDHTLKIWN VRSGQLDKI LTGHTDEIY 
      VLESHPHDR NVILSAGHD GQLFIWDLY KGEIISRFN 
      NTIENQGYG AIFDVKWSP DGNVVAASD SHGHLLIFG 
      FGTGDSLFE QLPKELFFH TDYRPLVRD TNNYVLDEQ 
      TQIPPHLMP PPFLVDIDG NPYPPMLQR LVPGREHCN 
      TEQLVPNVV IGNEGTQEV IQEAEVQPS VRNHLRPQN 
      HRNEGIRQS TGDWQSDPN MKWKKHVLV PPLKDTHIR 
      KIKEDVEMM ENMEMQEYK RQLCQRPHM ITVSTSAET 
      SKSIEKKRN PIRIRPTKE SINKPIFIV SASSSSESE 
      SDSTGYSDW VAEEDVNED VNNRESPKR TRRRQVNYN 
      SESDDHKDE SRENTPEKF PVRPMKIKD PERYRISEW 
      LTEVRPRKT PYFPQMGDE LVYFPQGHH LYLEAVRTK 
      KIYEINSKT LPWTKMHLR SHEFVKVIG IQYEVKPPR 
      LCNLRLALL DDDGRLNGK IFTVKYHDM PDVLDFFVL 
      KQHFDCAIS RNWEIGDKF RCMIDDGWW IGEIVAKNN 
      ASGDFPESP FLCYEIKWD NDELERMSP WDMEALDDN 
      RVPENSGEA LPVLEEELQ SILYQPTAD DWPNSDREA 
      VTCYILTGL AKVMELAIA EPFLVPVDL NTYPTYAVI 
      VEYPVDLST IKARFENRF YRRLTAAQF DVRHLAANA 
      EKFNERHSI IVKHARILT ELCLRIIKQ CSTHLDVTA 
      IYHQLVDTY DSTDTEVDV EIPPSSSRM LRSLPTRYL 
      RHASDWKSE AKILFQAIW DCEDSVPFR TPVNNLKYT 
      DYHDIIETP MDLSTVKDK LFSDKYETP NEFYNDMRL 
      IFQNSRTYN TNKRSRIYV MTVRLAAIF EEHISKLIK 
      HWKMAKRRS YATQQTNNK AESGTNSSS SSSELSEIE 
      EEMKNVESD SSDSEDNKP LKKLQTVKV TTKVVKSEE 
      ESESESSES VEKMNEDGS EEDESNDSE DSCYKPVTR 
      STRKRNEIT SSEEEEDEE ESSDDSDDK PLISVRHNG 
      RAKKRSRYV ESESEDDKE FFTSVSSRG RVRKLNPRV 
      TALMKK
    CDS sequence
    • >TC004357 RA
      atggataat ccggaagtt tgtccgata acagcagat 
      ctgtacttt ctgctccac aagtttctc tcaggtggc 
      ccccttaaa aaaacacta aaggcgctt catgaggaa 
      ttaaccgaa aataagtta ttgccacaa aggaccgac 
      tggttgggg aaattacat gatcgtact ttggaagat 
      ttggagcac caatacccc aatgttcga cccgattat 
      ttgttacaa ttgtgttgc cgtgccggc actcaaaaa 
      gtgactcaa gttgaaaaa atcgccaca cttttcagc 
      ttgaaaaaa catgccgtc aatagtagt aacgtagag 
      tacaatttt aagcaactt tgtaattat gtccagaga 
      cggcacttg ctccccttg tgcaaccgt gtccatatt 
      tcatcaaac ctcgtgaat atcttacgg ggtcgtgaa 
      atttcgggc ccaatttcc aggcgttgg gttgtcgcc 
      ccaaaacta tacagtggt ctacagttc cagagacgt 
      actttgggc cacctttcg gccgtttat tgtcttcta 
      tttgattat tcaggacgc tatataatc acaggtgct 
      gatgatctt ttgatcaaa ctttggtcg tctgttacc 
      ggacgtctt atcgcaact tttcgaggc gcctcctcc 
      gaaatcacc gacattgcc atcaaccta gaaaacacc 
      cttctggca gccggttca atagaccgt atcctccga 
      atctgggac ctacaaaca ggaatccca gttgctgtc 
      cttcccgga cacaccgga atgataact tcggtcaat 
      ttctgtcct tccccttct tggaatttt caatatttg 
      attacgaca agtacagat ggttcagtc gctttctgg 
      gcttacaca cacgaaccg ggtggaaaa gtaaatttc 
      catgcaaaa ccaacaatg tatcaagaa aaaatgcga 
      ccaggacaa gcacaaatg atttgttca tcgttcagt 
      ccaggtgga aattttctg gcaacagga tcggccgat 
      catcacgtg cgtgtctat tatatgaaa ggtgatgaa 
      ggtccccat cgaatactc gaaacggaa gcacacaac 
      gatcgagtc gactcgata caatgggcc catgaagga 
      cttaaattt ttatcggga agtaaagac ggaaccgcg 
      attatttgg agctttgag tcgcaacaa tggaaaacc 
      acctattta cacatgaca gccgctagc aatacagtg 
      aaacccaca agtacgaag aagatggcg gaagaggcg 
      cgaaaattg aaagtaaca atggtaaca tgggaccgt 
      tcagacgct tgggccata acagccgtc agtgaccat 
      acgcttaaa atatggaat gtgcgtagt ggccaacta 
      gataaaatt ctaactggt catactgat gagatttac 
      gtgcttgaa agtcatcca catgatcgt aatgtgatc 
      ttatcggct ggtcatgat ggtcaattg tttatctgg 
      gatttgtat aaaggtgaa ataatttcg cgttttaat 
      aatacgatt gagaatcaa ggttatggt gcaattttc 
      gatgtgaaa tggtcaccg gatggtaat gtggtggct 
      gcgtccgat tcacatgga catttattg atttttggt 
      tttggtact ggtgatagt ttgtttgag cagttgccc 
      aaagaattg ttttttcat acggattat cggccatta 
      gttcgtgat acaaataat tatgttttg gatgaacaa 
      acacaaatt ccaccacat ttaatgcca ccaccgttt 
      cttgtcgat attgatggt aatccgtac ccgccaatg 
      ttacaacgt ttagtacca ggacgggaa cattgtaac 
      actgaacag ttggtaccg aatgttgtg attggtaat 
      gaaggtact caagaggtg atacaagaa gctgaagta 
      cagccatct gttcgaaat catttgcgg ccgcaaaat 
      catcgaaat gaaggcatt agacaaagt accggtgat 
      tggcaaagt gatcctaac atgaaatgg aaaaaacac 
      gtccttgta ccaccgtta aaggacact catatcaga 
      aaaatcaaa gaggatgtc gaaatgatg gaaaatatg 
      gaaatgcag gaatataag agacagcta tgtcagcgc 
      cctcacatg atcacagtt agtactagt gccgaaacg 
      agcaaatcg attgaaaaa aaacgtaat ccgattcgt 
      attcgtcca acgaaagaa tcaataaac aaaccgatt 
      ttcatcgtt tcggcaagc agttcgtcg gaaagcgaa 
      agcgattca actggttat tctgattgg gtggctgaa 
      gaagacgtc aatgaagac gtcaacaac cgggaatcc 
      cctaaacgt acacgccgc cgccaagtc aattacaat 
      tcggaatcg gatgatcat aaagatgaa tcgcgtgaa 
      aatacaccg gaaaaattt ccagttcgg ccaatgaaa 
      atcaaagat cccgaacga taccgaatc agtgaatgg 
      ttgacggaa gtgcgaccg cgtaaaact ccgtatttt 
      cctcaaatg ggcgatgaa ctagtatat tttcctcaa 
      ggtcatcac ttgtatttg gaggcagtc cgtacgaaa 
      aaaatctac gaaattaat agtaaaaca ctgccgtgg 
      accaaaatg catttgagg agtcacgaa tttgttaaa 
      gtcattgga atccaatac gaagtgaaa ccgccacgt 
      ttgtgtaat ttacggttg gcactactg gacgacgat 
      ggtcgtctt aatggtaaa atttttacc gttaaatat 
      cacgatatg cctgatgtt ttggatttt ttcgtcttg 
      aaacaacat ttcgattgt gcaatttca cgaaattgg 
      gaaattggt gataaattt cgttgtatg attgacgat 
      ggttggtgg attggtgaa attgtggcg aaaaataac 
      gcaagtggg gacttcccc gaatcacca tttctctgt 
      tacgagatt aaatgggat aacgatgaa ttagaacga 
      atgagtcct tgggatatg gaagcgttg gatgataat 
      cgtgtacca gaaaattcc ggagaggca cttccagta 
      cttgaagaa gagttacaa agtattttg tatcaacca 
      acggccgat gattggccg aatagtgac agagaagcg 
      gtcacgtgt tatatttta accggttta gctaaagta 
      atggaattg gcgatagcc gagccgttt ttagtacct 
      gttgactta aacacttat ccaacttat gccgttatt 
      gtcgaatat ccggttgat ttatcgacg attaaagca 
      cgatttgaa aatcgattt tatcgcagg ttgactgct 
      gcacaattc gatgtgcgc catctagcg gcaaatgcg 
      gaaaaattc aacgaacgg catagtatt atcgtgaaa 
      catgcaaga atcttaaca gaattatgt ctacgaata 
      atcaaacaa tgttcaact catcttgac gtaactgca 
      atttatcat caattagtc gacacttat gactcgacc 
      gatactgaa gtcgacgtt gaaatacct ccatctagt 
      agtcgtatg ttacgttca ttgcctact agatattta 
      agacatgct agtgattgg aaatcggaa gcgaaaatt 
      ttattccaa gcgatttgg gattgtgag gattcagtg 
      ccttttcgt acacctgtc aataattta aaatataca 
      gattaccac gatataatc gagacaccc atggatttg 
      agtacagtc aaagacaaa ctattcagt gataaatac 
      gaaactccc aatgagttt tataatgat atgcgatta 
      atttttcaa aattcgcgc acttataat acaaataag 
      cgatcaagg atttatgtt atgacggta cggcttgct 
      gcgattttc gaggaacat ataagtaaa ttaataaaa 
      cattggaag atggcgaaa cggcgaagt tatgccaca 
      caacaaacc aataataag gcagaaagt ggtacaaat 
      tcatcgtca tcttcatcc gaattgagt gagattgag 
      gaagaaatg aagaatgta gagagtgat agtagcgat 
      agtgaggat aataaaccg cttaagaaa ttacaaact 
      gtaaaagtg actacgaaa gttgttaaa tccgaggaa 
      gaaagtgag agtgaatca agcgagagt gtggaaaaa 
      atgaatgaa gatggtagt gaggaagac gaatccaat 
      gattcggaa gatagttgt tataaacca gtgacgcga 
      agtacaaga aaacgaaat gaaattaca tcgtcagaa 
      gaagaagaa gatgaagaa gaatcgagt gatgatagc 
      gatgataaa cctttgatt tcagttaga cataatgga 
      cgtgcaaag aaacggtcg cgttatgtt gagagtgaa 
      agtgaggat gataaggaa ttttttaca agtgtaagt 
      agcagaggc cgagtgagg aaattaaat cctagggtc 
      actgccttg atgaaaaaa tag
    mRNA sequence
    • >TC004357 RA
      tgctagtga cagttgaaa aagtatcgt gaggttatg 
      tagcttaat ttatagttt atttacgaa gaaaatgga 
      taatccgga agtttgtcc gataacagc agatctgta 
      ctttctgct ccacaagtt tctctcagg tggccccct 
      taaaaaaac actaaaggc gcttcatga ggaattaac 
      cgaaaataa gttattgcc acaaaggac cgactggtt 
      ggggaaatt acatgatcg tactttgga agatttgga 
      gcaccaata ccccaatgt tcgacccga ttatttgtt 
      acaattgtg ttgccgtgc cggcactca aaaagtgac 
      tcaagttga aaaaatcgc cacactttt cagcttgaa 
      aaaacatgc cgtcaatag tagtaacgt agagtacaa 
      ttttaagca actttgtaa ttatgtcca gagacggca 
      cttgctccc cttgtgcaa ccgtgtcca tatttcatc 
      aaacctcgt gaatatctt acggggtcg tgaaatttc 
      gggcccaat ttccaggcg ttgggttgt cgccccaaa 
      actatacag tggtctaca gttccagag acgtacttt 
      gggccacct ttcggccgt ttattgtct tctatttga 
      ttattcagg acgctatat aatcacagg tgctgatga 
      tcttttgat caaactttg gtcgtctgt taccggacg 
      tcttatcgc aacttttcg aggcgcctc ctccgaaat 
      caccgacat tgccatcaa cctagaaaa cacccttct 
      ggcagccgg ttcaataga ccgtatcct ccgaatctg 
      ggacctaca aacaggaat cccagttgc tgtccttcc 
      cggacacac cggaatgat aacttcggt caatttctg 
      tccttcccc ttcttggaa ttttcaata tttgattac 
      gacaagtac agatggttc agtcgcttt ctgggctta 
      cacacacga accgggtgg aaaagtaaa tttccatgc 
      aaaaccaac aatgtatca agaaaaaat gcgaccagg 
      acaagcaca aatgatttg ttcatcgtt cagtccagg 
      tggaaattt tctggcaac aggatcggc cgatcatca 
      cgtgcgtgt ctattatat gaaaggtga tgaaggtcc 
      ccatcgaat actcgaaac ggaagcaca caacgatcg 
      agtcgactc gatacaatg ggcccatga aggacttaa 
      atttttatc gggaagtaa agacggaac cgcgattat 
      ttggagctt tgagtcgca acaatggaa aaccaccta 
      tttacacat gacagccgc tagcaatac agtgaaacc 
      cacaagtac gaagaagat ggcggaaga ggcgcgaaa 
      attgaaagt aacaatggt aacatggga ccgttcaga 
      cgcttgggc cataacagc cgtcagtga ccatacgct 
      taaaatatg gaatgtgcg tagtggcca actagataa 
      aattctaac tggtcatac tgatgagat ttacgtgct 
      tgaaagtca tccacatga tcgtaatgt gatcttatc 
      ggctggtca tgatggtca attgtttat ctgggattt 
      gtataaagg tgaaataat ttcgcgttt taataatac 
      gattgagaa tcaaggtta tggtgcaat tttcgatgt 
      gaaatggtc accggatgg taatgtggt ggctgcgtc 
      cgattcaca tggacattt attgatttt tggttttgg 
      tactggtga tagtttgtt tgagcagtt gcccaaaga 
      attgttttt tcatacgga ttatcggcc attagttcg 
      tgatacaaa taattatgt tttggatga acaaacaca 
      aattccacc acatttaat gccaccacc gtttcttgt 
      cgatattga tggtaatcc gtacccgcc aatgttaca 
      acgtttagt accaggacg ggaacattg taacactga 
      acagttggt accgaatgt tgtgattgg taatgaagg 
      tactcaaga ggtgataca agaagctga agtacagcc 
      atctgttcg aaatcattt gcggccgca aaatcatcg 
      aaatgaagg cattagaca aagtaccgg tgattggca 
      aagtgatcc taacatgaa atggaaaaa acacgtcct 
      tgtaccacc gttaaagga cactcatat cagaaaaat 
      caaagagga tgtcgaaat gatggaaaa tatggaaat 
      gcaggaata taagagaca gctatgtca gcgccctca 
      catgatcac agttagtac tagtgccga aacgagcaa 
      atcgattga aaaaaaacg taatccgat tcgtattcg 
      tccaacgaa agaatcaat aaacaaacc gattttcat 
      cgtttcggc aagcagttc gtcggaaag cgaaagcga 
      ttcaactgg ttattctga ttgggtggc tgaagaaga 
      cgtcaatga agacgtcaa caaccggga atcccctaa 
      acgtacacg ccgccgcca agtcaatta caattcgga 
      atcggatga tcataaaga tgaatcgcg tgaaaatac 
      accggaaaa atttccagt tcggccaat gaaaatcaa 
      agatcccga acgataccg aatcagtga atggttgac 
      ggaagtgcg accgcgtaa aactccgta ttttcctca 
      aatgggcga tgaactagt atattttcc tcaaggtca 
      tcacttgta tttggaggc agtccgtac gaaaaaaat 
      ctacgaaat taatagtaa aacactgcc gtggaccaa 
      aatgcattt gaggagtca cgaatttgt taaagtcat 
      tggaatcca atacgaagt gaaaccgcc acgtttgtg 
      taatttacg gttggcact actggacga cgatggtcg 
      tcttaatgg taaaatttt taccgttaa atatcacga 
      tatgcctga tgttttgga ttttttcgt cttgaaaca 
      acatttcga ttgtgcaat ttcacgaaa ttgggaaat 
      tggtgataa atttcgttg tatgattga cgatggttg 
      gtggattgg tgaaattgt ggcgaaaaa taacgcaag 
      tggggactt ccccgaatc accatttct ctgttacga 
      gattaaatg ggataacga tgaattaga acgaatgag 
      tccttggga tatggaagc gttggatga taatcgtgt 
      accagaaaa ttccggaga ggcacttcc agtacttga 
      agaagagtt acaaagtat tttgtatca accaacggc 
      cgatgattg gccgaatag tgacagaga agcggtcac 
      gtgttatat tttaaccgg tttagctaa agtaatgga 
      attggcgat agccgagcc gtttttagt acctgttga 
      cttaaacac ttatccaac ttatgccgt tattgtcga 
      atatccggt tgatttatc gacgattaa agcacgatt 
      tgaaaatcg attttatcg caggttgac tgctgcaca 
      attcgatgt gcgccatct agcggcaaa tgcggaaaa 
      attcaacga acggcatag tattatcgt gaaacatgc 
      aagaatctt aacagaatt atgtctacg aataatcaa 
      acaatgttc aactcatct tgacgtaac tgcaattta 
      tcatcaatt agtcgacac ttatgactc gaccgatac 
      tgaagtcga cgttgaaat acctccatc tagtagtcg 
      tatgttacg ttcattgcc tactagata tttaagaca 
      tgctagtga ttggaaatc ggaagcgaa aattttatt 
      ccaagcgat ttgggattg tgaggattc agtgccttt 
      tcgtacacc tgtcaataa tttaaaata tacagatta 
      ccacgatat aatcgagac acccatgga tttgagtac 
      agtcaaaga caaactatt cagtgataa atacgaaac 
      tcccaatga gttttataa tgatatgcg attaatttt 
      tcaaaattc gcgcactta taatacaaa taagcgatc 
      aaggattta tgttatgac ggtacggct tgctgcgat 
      tttcgagga acatataag taaattaat aaaacattg 
      gaagatggc gaaacggcg aagttatgc cacacaaca 
      aaccaataa taaggcaga aagtggtac aaattcatc 
      gtcatcttc atccgaatt gagtgagat tgaggaaga 
      aatgaagaa tgtagagag tgatagtag cgatagtga 
      ggataataa accgcttaa gaaattaca aactgtaaa 
      agtgactac gaaagttgt taaatccga ggaagaaag 
      tgagagtga atcaagcga gagtgtgga aaaaatgaa 
      tgaagatgg tagtgagga agacgaatc caatgattc 
      ggaagatag ttgttataa accagtgac gcgaagtac 
      aagaaaacg aaatgaaat tacatcgtc agaagaaga 
      agaagatga agaagaatc gagtgatga tagcgatga 
      taaaccttt gatttcagt tagacataa tggacgtgc 
      aaagaaacg gtcgcgtta tgttgagag tgaaagtga 
      ggatgataa ggaattttt tacaagtgt aagtagcag 
      aggccgagt gaggaaatt aaatcctag ggtcactgc 
      cttgatgaa aaaatagta tttataagt ttatattta 
      tttgtttat tgtttttat actgtcaat aaagctaaa 
      ttatcacg
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