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Gene information
General information
TC004355 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 7217372..7221833
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004355
    iBeetle screen iB_03732

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect, flour poorly removed,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    labium not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004355 RA
      MMSCMNAAA ELDHGCALR PGCPSPTST PEELERRAL 
      IQRNDLLLH EAVIKNDTE CVRRILKEP VDINSRNNY 
      GRAPIHWAA SRGNIDIME MLIAANCDI EARDKYGMR 
      PLLMACWHG HRDAVQLLI NTGACSNAT NKKHYTLLM 
      CAARNNRIS VVDFLIDTL EDTRVDAVD IDSQTALFH 
      AAMGGHIEI VKRLIDLGA NLNKRSKES RTALHVACE 
      RGHCDVAEL LLNHEADME AKDTNGNTP LHVASQNQQ 
      TELVHVLLE TGADPDSEN LKGSTPLHI ASSLGSKGI 
      LEILLQHGA SINKQNKNG NTSLHIACQ ANVMEMVEI 
      LISKGANLN SLNARLQSP IHIAAEMGH TEICKVLLA 
      AGADIEQRE QGGRTPLYI AARGSFTAI VDMIIKTAR 
      LDYPAHEDP NERKDNNGF KQSLSTARS KWRSTKEDI 
      RGRDNNERL KEILYKVAY KQLGPGEWK MLAHHWAFT 
      DEQIKAIEH QYNGPSSYK EHGFRMMLI WSHGLGPDV 
      NPIRELYES LTAVGKRPL ADALRRKID QENETRRKG 
      NQQNCCTLC
    CDS sequence
    • >TC004355 RA
      atgatgtct tgtatgaat gctgctgct gaattagac 
      cacggttgt gctttaaga cctggttgt ccatcacct 
      accagcacc cccgaagaa ttggaaaga agagcttta 
      attcaaaga aatgattta ctgttacac gaagcggtc 
      attaaaaat gatacagaa tgtgtgaga agaatcttg 
      aaagaacca gtcgatata aactctaga aacaattat 
      ggaagagct cctattcac tgggctgcc tctcgcggt 
      aacatcgac attatggaa atgctaata gcagccaat 
      tgtgacatt gaagctcgc gacaagtac ggaatgaga 
      cctttacta atggcatgt tggcatgga catcgcgat 
      gctgttcaa ttgcttatc aacacagga gcttgctct 
      aatgctacc aacaagaaa cactatact ttgttgatg 
      tgtgcagcc cgaaataat cgaatatca gttgtcgat 
      tttctaatt gatactttg gaggatacg cgagtagat 
      gcggttgat attgatagt caaactgcc ctatttcac 
      gctgcaatg ggtggtcac atcgaaatt gtcaaacgt 
      ctcattgat ttgggagca aacttgaat aaacgaagc 
      aaagaaagc agaactgct ctacatgtg gcttgcgaa 
      agaggccat tgtgatgtt gccgaactt cttctcaat 
      cacgaagct gacatggaa gctaaagat acaaacgga 
      aacactccc cttcatgtt gcttctcaa aatcaacaa 
      actgaatta gttcatgta ctcctagag acgggagca 
      gatcctgat agcgaaaat ttgaaagga tctactcca 
      ttgcacata gcgagtagt ttgggaagt aaaggaatt 
      ttagaaatt cttttacag catggagct tccatcaat 
      aaacaaaat aagaacgga aatacatct ttacatata 
      gcttgtcaa gcaaatgtt atggaaatg gttgaaatt 
      cttataagc aagggagca aatctcaat agtctaaac 
      gcgagatta caatcacct attcatatt gccgcagaa 
      atgggtcat acggaaatt tgtaaagtg cttttagcg 
      gcaggtgca gatatcgaa cagcgagaa caaggagga 
      aggactcca ctttatata gcagcaaga ggaagtttc 
      accgctatt gtcgatatg atcattaaa acggcacga 
      ttggactat cccgcacac gaagatcca aacgaaaga 
      aaagataat aacggtttt aagcaatcg ttaagtacg 
      gcgagaagt aaatggcga tcgacgaaa gaagacatc 
      cgaggcaga gacaataat gaaagactc aaggaaatt 
      ctctataaa gtggcttac aaacagctg ggaccgggt 
      gaatggaaa atgttagcg catcactgg gctttcacc 
      gatgaacaa atcaaagcg atcgaacat caatacaac 
      ggaccatcc agttataaa gaacacgga tttcgaatg 
      atgttaatt tggagtcac ggattaggt cctgatgtt 
      aatccaatt agagaactt tacgaaagt ctaacggcc 
      gttgggaaa agaccgctc gctgacgca ctccgaaga 
      aaaatcgat caagaaaac gaaacaaga cgaaaaggc 
      aatcagcaa aattgttgc actttatgt tag
    mRNA sequence
    • >TC004355 RA
      cgcttgcgt acatattat gacacgcgg tgaatgtag 
      acgtcgcga cggcgaccc tttcagtta cagttcgaa 
      taagcaacg tacgtacaa gtgttttat cagactact 
      tcgtcaaca ggaggccaa tatttaaaa gaaaaatga 
      ttcgattat gaaaatgta attaaaagg aaataagaa 
      atgatgtct tgtatgaat gctgctgct gaattagac 
      cacggttgt gctttaaga cctggttgt ccatcacct 
      accagcacc cccgaagaa ttggaaaga agagcttta 
      attcaaaga aatgattta ctgttacac gaagcggtc 
      attaaaaat gatacagaa tgtgtgaga agaatcttg 
      aaagaacca gtcgatata aactctaga aacaattat 
      ggaagagct cctattcac tgggctgcc tctcgcggt 
      aacatcgac attatggaa atgctaata gcagccaat 
      tgtgacatt gaagctcgc gacaagtac ggaatgaga 
      cctttacta atggcatgt tggcatgga catcgcgat 
      gctgttcaa ttgcttatc aacacagga gcttgctct 
      aatgctacc aacaagaaa cactatact ttgttgatg 
      tgtgcagcc cgaaataat cgaatatca gttgtcgat 
      tttctaatt gatactttg gaggatacg cgagtagat 
      gcggttgat attgatagt caaactgcc ctatttcac 
      gctgcaatg ggtggtcac atcgaaatt gtcaaacgt 
      ctcattgat ttgggagca aacttgaat aaacgaagc 
      aaagaaagc agaactgct ctacatgtg gcttgcgaa 
      agaggccat tgtgatgtt gccgaactt cttctcaat 
      cacgaagct gacatggaa gctaaagat acaaacgga 
      aacactccc cttcatgtt gcttctcaa aatcaacaa 
      actgaatta gttcatgta ctcctagag acgggagca 
      gatcctgat agcgaaaat ttgaaagga tctactcca 
      ttgcacata gcgagtagt ttgggaagt aaaggaatt 
      ttagaaatt cttttacag catggagct tccatcaat 
      aaacaaaat aagaacgga aatacatct ttacatata 
      gcttgtcaa gcaaatgtt atggaaatg gttgaaatt 
      cttataagc aagggagca aatctcaat agtctaaac 
      gcgagatta caatcacct attcatatt gccgcagaa 
      atgggtcat acggaaatt tgtaaagtg cttttagcg 
      gcaggtgca gatatcgaa cagcgagaa caaggagga 
      aggactcca ctttatata gcagcaaga ggaagtttc 
      accgctatt gtcgatatg atcattaaa acggcacga 
      ttggactat cccgcacac gaagatcca aacgaaaga 
      aaagataat aacggtttt aagcaatcg ttaagtacg 
      gcgagaagt aaatggcga tcgacgaaa gaagacatc 
      cgaggcaga gacaataat gaaagactc aaggaaatt 
      ctctataaa gtggcttac aaacagctg ggaccgggt 
      gaatggaaa atgttagcg catcactgg gctttcacc 
      gatgaacaa atcaaagcg atcgaacat caatacaac 
      ggaccatcc agttataaa gaacacgga tttcgaatg 
      atgttaatt tggagtcac ggattaggt cctgatgtt 
      aatccaatt agagaactt tacgaaagt ctaacggcc 
      gttgggaaa agaccgctc gctgacgca ctccgaaga 
      aaaatcgat caagaaaac gaaacaaga cgaaaaggc 
      aatcagcaa aattgttgc actttatgt tagaaacat 
      aactttttc ttatgaaaa tacgacata tctcaatgg 
      tccaaaaaa caacaaaat tcatgtaag cagtcgagg 
      tcaccaaac taaataccg gctgactat aaaaaattg 
      tgtcacaaa aatataatg ccataccaa gatttagcg 
      acatgaaat acattcttc aataatcat tcggtataa 
      aaaatgtat ggtgaccat ccacaaaga aaacatttt 
      agagaataa ataaatttg gtacaattt tgaacaaaa 
      ggtaatcga acaaaaagg actgtgata ttattgtag 
      gtaaaatat tttagtttt tagtatcta ccattatta 
      cctatgcaa caattttcg tgccaaaat agtgttttt 
      tgttaaaaa aaatatata tacctattt gcttaccta 
      caacaaaaa tgcaactac caaaacttt gtaaaaagt 
      gatttgatt taaaaataa ataaagttc ttattaatc 
      a
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