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Gene information
General information
TC004348 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451782.1: 2985..10633
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004348
      iBeetle screen iB_03730

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004348 RA
        MVTSVVSVP VPQLGQLED CFEEDEIEC SQLSVKEKQ 
        LANHAVANI VQIVDEDDD DSMVIPLSQ PDSELSFSL 
        PFTQYLAQH GIENDYSDD ISLTLIDQF INEDEAVSN 
        PVVDEGTCP INNSITDEL NVFDNDMVV VVEEPPISP 
        SPPLPPQSP FVEPVFTRP VTLTSPEAA PAILTQPPM 
        IISQDIIDA LSEPWVEVE NIINGNVEH PPQPNSPII 
        EENDVLPER LVVVEAENN IIRSPQPSP PPSPIILSQ 
        GVNDMQQPS VIDALAEPW VEERRQGEE MDVENDIIQ 
        PSQLSPILG QINNRIENR VEGGDDDDD DDIIPPTPP 
        HSRSRRRRR PRKPLILKK ALMPIKVNR LLSSNEAAV 
        VVFQFSYHQ HVNSIRDFA TYLQYNDCR LKVMNNQLR 
        QVVSNQYQK ELRVVVFFA HWL
      CDS sequence
      • >TC004348 RA
        atggtgacg agtgttgtc agtgtcccg gtccctcaa 
        ctaggacaa ctggaagat tgttttgaa gaggatgag 
        attgaatgt agtcagtta agtgtgaaa gagaagcaa 
        ttagctaat cacgctgtt gctaatatt gttcaaatc 
        gttgatgag gatgatgat gattctatg gttattcct 
        ttatcacaa cctgatagt gaattatcg ttttcttta 
        ccattcacg caatacctg gctcaacat ggtattgaa 
        aatgattat tcggacgac atctcgttg accttaata 
        gaccagttt attaacgaa gacgaggcg gtgtccaat 
        ccggttgtg gatgaagga acttgtccc atcaataat 
        tcgattacc gatgagtta aatgtattt gataatgat 
        atggtggtg gtggtggaa gaaccccca atctctccc 
        agtccacca ctaccacca caaagcccc ttcgttgag 
        ccagtgttt acccgcccc gttacacta acatctcct 
        gaagcggca cccgcgata ttaactcaa ccccctatg 
        ataatatcg caggacatc attgacgct ttatcggag 
        ccttgggtt gaggtggaa aacattatc aatggtaac 
        gttgaacat ccccctcaa cctaattca ccgattatt 
        gaggaaaat gatgttctg ccagagaga ttggtggtg 
        gtagaggcc gaaaataac atcattcgg tcaccacaa 
        ccatcacca ccaccgtca cccataata ttatcacag 
        ggcgtcaat gacatgcaa caaccatct gtcattgac 
        gcactggca gagccgtgg gttgaagag aggaggcag 
        ggtgaagag atggatgtt gaaaatgac atcattcaa 
        ccctctcaa ttgtcaccc attctaggg caaataaat 
        aataggatt gagaatagg gtggagggt ggagacgat 
        gacgacgac gatgacatc attccaccc actccccct 
        cactcacga tcaaggagg cgtcggcgc cctagaaag 
        cctctgata ctgaagaaa gccttgatg ccgatcaag 
        gtcaatcga ctcctgtcc agcaacgaa gccgctgta 
        gtagtcttt caattctcg taccaccag cacgtaaac 
        agtatccgc gacttcgca acatacctg caatacaac 
        gattgcagg ctcaaggtc atgaacaat caactccga 
        caagtagta tccaaccaa tatcaaaaa gagttaagg 
        gtggtggta ttcttcgca cattggctt taa
      mRNA sequence
      • >TC004348 RA
        ttcattctg tgttgtaca ggtagtctg tgtacgaaa 
        taatgtgtt caaattatt ttgctaatt ttaaaccgt 
        gcactcatg gtgacgagt gttgtcagt gtcccggtc 
        cctcaacta ggacaactg gaagattgt tttgaagag 
        gatgagatt gaatgtagt cagttaagt gtgaaagag 
        aagcaatta gctaatcac gctgttgct aatattgtt 
        caaatcgtt gatgaggat gatgatgat tctatggtt 
        attccttta tcacaacct gatagtgaa ttatcgttt 
        tctttacca ttcacgcaa tacctggct caacatggt 
        attgaaaat gattattcg gacgacatc tcgttgacc 
        ttaatagac cagtttatt aacgaagac gaggcggtg 
        tccaatccg gttgtggat gaaggaact tgtcccatc 
        aataattcg attaccgat gagttaaat gtatttgat 
        aatgatatg gtggtggtg gtggaagaa cccccaatc 
        tctcccagt ccaccacta ccaccacaa agccccttc 
        gttgagcca gtgtttacc cgccccgtt acactaaca 
        tctcctgaa gcggcaccc gcgatatta actcaaccc 
        cctatgata atatcgcag gacatcatt gacgcttta 
        tcggagcct tgggttgag gtggaaaac attatcaat 
        ggtaacgtt gaacatccc cctcaacct aattcaccg 
        attattgag gaaaatgat gttctgcca gagagattg 
        gtggtggta gaggccgaa aataacatc attcggtca 
        ccacaacca tcaccacca ccgtcaccc ataatatta 
        tcacagggc gtcaatgac atgcaacaa ccatctgtc 
        attgacgca ctggcagag ccgtgggtt gaagagagg 
        aggcagggt gaagagatg gatgttgaa aatgacatc 
        attcaaccc tctcaattg tcacccatt ctagggcaa 
        ataaataat aggattgag aatagggtg gagggtgga 
        gacgatgac gacgacgat gacatcatt ccacccact 
        ccccctcac tcacgatca aggaggcgt cggcgccct 
        agaaagcct ctgatactg aagaaagcc ttgatgccg 
        atcaaggtc aatcgactc ctgtccagc aacgaagcc 
        gctgtagta gtctttcaa ttctcgtac caccagcac 
        gtaaacagt atccgcgac ttcgcaaca tacctgcaa 
        tacaacgat tgcaggctc aaggtcatg aacaatcaa 
        ctccgacaa gtagtatcc aaccaatat caaaaagag 
        ttaagggtg gtggtattc ttcgcacat tggctttaa 
        cagataaaa atttagaat ttgttccct acaaaaatc 
        ccaacacac tcgtaaata gactacgtc ttttattat 
        catcgacag tagctggta ataataatc atatgaatg 
        aaat
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY