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Gene information
General information
TC004339 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451782.1: 40394..43713
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004339
    iBeetle screen iB_03729

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004339 RA
      MGSSRRNST TSTSDQLDH ENSGDDIAN IIEDCSKNL 
      KVLDSFILK SKESIRERQ TYQTAIQNI QSGLRNILL 
      SFRKPSFES DIENKIASI VEKVIDNKI NNLNPNSSP 
      IGPRYSQIL KSNLTKSTE PVTAPAKLP QSNYKVIVK 
      PNESLKNVK SSEDTRKIL TSKSPHEFG IKVNKIVPI 
      RNNAILIES TCSSILNLT DSPVLKSLN LQAERINKV 
      WPKIQVFDV PKEMVADEL IKEIQKQPD LPGNIPKQF 
      VKSAFKAGN KEGKTNHWV IEIHPAARN YFIASGSKL 
      FVSWKSLHV RDYLRITRC FKCQKFGHV SKFCNSEKQ 
      CGYCASNDH ESISCNFKN NEDKHKCSN CERAGQQEL 
      NHPAGSTNC PIYKHRMQE ALKNIDFDG
    CDS sequence
    • >TC004339 RA
      atggggtcg tcacgtcga aactcgacg acgtcgaca 
      agcgatcaa ttggatcat gaaaactcc ggcgatgat 
      atcgcaaat attattgaa gactgctca aaaaattta 
      aaagtttta gatagtttc attctgaaa tcgaaagag 
      tctatccgc gagagacaa acttatcaa acggctata 
      caaaacata caatccggc ctaagaaat attttatta 
      agcttcaga aaacctagt tttgaatcc gatattgaa 
      aataagata gccagcatt gtggaaaaa gtcattgat 
      aataaaata aataattta aacccaaat tcgtcgccg 
      attggaccg agatacagc cagatttta aagtcaaac 
      ttaacaaaa agcactgaa cccgtcaca gccccggca 
      aagctccct cagagcaac tataaagta attgtcaaa 
      cccaatgaa agtctaaaa aatgtgaaa tcatccgaa 
      gacacgaga aaaatatta acatccaag tcaccgcat 
      gaatttggc attaaagtg aataaaatc gtcccgatt 
      agaaataat gcaatttta attgagtcc acgtgttcg 
      tctattttg aacttaact gatagccct gtcctcaaa 
      tctttaaat ttacaagcg gaaagaatt aataaagtg 
      tggccaaaa attcaagtt tttgatgtc ccgaaagaa 
      atggtggcc gacgagcta attaaggaa attcaaaag 
      caaccagat ttaccggga aatattcca aaacaattt 
      gtaaaatcg gctttcaaa gcagggaat aaagaggga 
      aaaacaaac cattgggtt attgaaatt catcccgct 
      gcgcggaat tactttatc gcatcaggt agcaaattg 
      ttcgtaagc tggaaatcc ctgcatgtt agagattac 
      ctgagaata acaagatgc tttaaatgt cagaaattt 
      gggcacgtt tcaaaattt tgcaactct gaaaaacag 
      tgtggttac tgtgccagc aatgatcat gagtcaatt 
      tcttgtaat ttcaaaaat aatgaggac aaacacaaa 
      tgcagtaat tgtgaaaga gccggccaa caagaactt 
      aatcatcca gcagggtca accaattgt cccatttac 
      aaacaccgg atgcaagag gccctaaaa aacatcgac 
      tttgatgga taa
    mRNA sequence
    • >TC004339 RA
      tgtatattt attaaatag aaaatatgt cgaaaaacc 
      agacgtttt cttcaaaca aacgccaca gctacaaac 
      ttaatcaca acaatattt accaaacaa tacaattgt 
      agggtgttt tgtgtgata cgtcgtttg gtgggcatt 
      ctaagaacg tccgtcgac ataaaaacg aatcaaaag 
      aaaagcaca ggcagtgca aaacgttcc ttcgtcagt 
      cgggcgtat atcatttaa aacccacag aatctaaaa 
      aagttaata aataaactt ttttattta gattcctac 
      ggttcgtcg cgtcgttaa agtaaatta ttacttcga 
      cgcatgcgg aacccaggt acccgaaaa ccggctttt 
      atttaaaaa tggggtcgt cacgtcgaa actcgacga 
      cgtcgacaa gcgatcaat tggatcatg aaaactccg 
      gcgatgata tcgcaaata ttattgaag actgctcaa 
      aaaatttaa aagttttag atagtttca ttctgaaat 
      cgaaagagt ctatccgcg agagacaaa cttatcaaa 
      cggctatac aaaacatac aatccggcc taagaaata 
      ttttattaa gcttcagaa aacctagtt ttgaatccg 
      atattgaaa ataagatag ccagcattg tggaaaaag 
      tcattgata ataaaataa ataatttaa acccaaatt 
      cgtcgccga ttggaccga gatacagcc agattttaa 
      agtcaaact taacaaaaa gcactgaac ccgtcacag 
      ccccggcaa agctccctc agagcaact ataaagtaa 
      ttgtcaaac ccaatgaaa gtctaaaaa atgtgaaat 
      catccgaag acacgagaa aaatattaa catccaagt 
      caccgcatg aatttggca ttaaagtga ataaaatcg 
      tcccgatta gaaataatg caattttaa ttgagtcca 
      cgtgttcgt ctattttga acttaactg atagccctg 
      tcctcaaat ctttaaatt tacaagcgg aaagaatta 
      ataaagtgt ggccaaaaa ttcaagttt ttgatgtcc 
      cgaaagaaa tggtggccg acgagctaa ttaaggaaa 
      ttcaaaagc aaccagatt taccgggaa atattccaa 
      aacaatttg taaaatcgg ctttcaaag cagggaata 
      aagagggaa aaacaaacc attgggtta ttgaaattc 
      atcccgctg cgcggaatt actttatcg catcaggta 
      gcaaattgt tcgtaagct ggaaatccc tgcatgtta 
      gagattacc tgagaataa caagatgct ttaaatgtc 
      agaaatttg ggcacgttt caaaatttt gcaactctg 
      aaaaacagt gtggttact gtgccagca atgatcatg 
      agtcaattt cttgtaatt tcaaaaata atgaggaca 
      aacacaaat gcagtaatt gtgaaagag ccggccaac 
      aagaactta atcatccag cagggtcaa ccaattgtc 
      ccatttaca aacaccgga tgcaagagg ccctaaaaa 
      acatcgact ttgatggat aacacaatt aatataatt 
      cagctaaac tgcaatggc gcgaaattg gcaacggca 
      gaattagtt aaatatgca cttgataat aatttccaa 
      ttggcattt ttacaagag ccgcatagg gtcaaatac 
      gggcaaaat tacagattg tcaggaatc tcgaccgca 
      attagctgc tttgcaact aacgatcca aaattccaa 
      acggcaatt ttgtgtttt gacccaagt ttaaatccc 
      ttattttac cctcaactt tcaaataaa ttcttaaca 
      ttact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY