Status
Please wait ...
Gene information
General information
TC004298 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451971.1: 30226..35513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004298
      iBeetle screen iB_03722

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004298 RA
        MRFLVLSIL ALSSLSQAQ ESAQYLHNA IKDLRYMKG 
        RQTLKRVDT TAVQAIVPD PEPTSAPAP APKAAERRM 
        QGGKSVPPL SLTKGELAA LYEAAVSKG ETVKLNTGD 
        NSYIHAAVH EMSDSPSYD HETKAGSGT DVNEGDDGT 
        GYYYYYYPV KSFLDDMAS QSTSHDYQY SSTNHKDTK 
        HTSEKPHYH QYHSHTHQH NKALEPLFM AISGFIGMA 
        VMFVLSVLV FPKFGIKKT RGNIKGGIK KNERITDIA 
        RVALQAIEG KDCAERFAC ELEKTARAF NLQDNRFVK 
        LLKRMAPSS FGKQMDKVG RYNNKRLRC TAIPCKKKN 
        PPKKKAGTQ KKP
      CDS sequence
      • >TC004298 RA
        atgcgattc ctcgtgtta tcaatcctg gccctctcc 
        agcctgtcg caagctcaa gaaagtgct caatacctt 
        cacaatgcc ataaaggac ttaagatat atgaaaggt 
        cgccagaca ctgaaacga gtggacaca acagccgtt 
        caggcaata gtccccgac cccgagcca acatcagcc 
        ccagccccg gctccaaaa gcggccgaa cgacgcatg 
        caagggggc aaaagtgtc ccacctttg tccctcaca 
        aagggcgaa cttgcagct ctgtacgaa gctgcagtc 
        tcaaaaggc gaaactgtc aagttaaac acaggggac 
        aactcttac atacatgca gccgtgcac gaaatgagt 
        gattccccg agttatgac cacgaaacc aaagccgga 
        agtggcaca gacgtgaac gaaggggat gacgggaca 
        gggtattat tactactat taccctgtg aagagcttc 
        ttagatgat atggcctcg caatcgact tcgcacgac 
        taccaatac agctcgacg aatcacaag gataccaag 
        cacacgtcc gaaaaaccc cactatcat cagtaccat 
        tcacacacg caccagcac aataaggcg ctcgagccc 
        ctatttatg gcaatttct ggcttcatt ggaatggca 
        gtgatgttc gttttatcg gtgcttgtg ttcccgaaa 
        ttcgggatt aaaaaaacc cgcgggaac ataaagggc 
        gggattaag aaaaacgaa cggattact gacatcgca 
        cgcgttgcc ctgcaagcc atcgagggg aaggattgt 
        gccgaaagg ttcgcctgc gagttggag aaaacggct 
        cgagcgttt aatctgcag gataatcga tttgtgaag 
        ttgttgaag cgaatggcg cccagttcg ttcgggaaa 
        caaatggat aaggttggg aggtataac aataaaaga 
        cttaggtgc actgcgatt ccttgtaaa aagaaaaat 
        ccgccgaag aagaaggca ggcacccag aagaaaccc 
        taa
      mRNA sequence
      • >TC004298 RA
        tgtgatgtt ttgtgtaca gagatgact agaatgcga 
        ttcctcgtg ttatcaatc ctggccctc tccagcctg 
        tcgcaagct caagaaagt gctcaatac cttcacaat 
        gccataaag gacttaaga tatatgaaa ggtcgccag 
        acactgaaa cgagtggac acaacagcc gttcaggca 
        atagtcccc gaccccgag ccaacatca gccccagcc 
        ccggctcca aaagcggcc gaacgacgc atgcaaggg 
        ggcaaaagt gtcccacct ttgtccctc acaaagggc 
        gaacttgca gctctgtac gaagctgca gtctcaaaa 
        ggcgaaact gtcaagtta aacacaggg gacaactct 
        tacatacat gcagccgtg cacgaaatg agtgattcc 
        ccgagttat gaccacgaa accaaagcc ggaagtggc 
        acagacgtg aacgaaggg gatgacggg acagggtat 
        tattactac tattaccct gtgaagagc ttcttagat 
        gatatggcc tcgcaatcg acttcgcac gactaccaa 
        tacagctcg acgaatcac aaggatacc aagcacacg 
        tccgaaaaa ccccactat catcagtac cattcacac 
        acgcaccag cacaataag gcgctcgag cccctattt 
        atggcaatt tctggcttc attggaatg gcagtgatg 
        ttcgtttta tcggtgctt gtgttcccg aaattcggg 
        attaaaaaa acccgcggg aacataaag ggcgggatt 
        aagaaaaac gaacggatt actgacatc gcacgcgtt 
        gccctgcaa gccatcgag gggaaggat tgtgccgaa 
        aggttcgcc tgcgagttg gagaaaacg gctcgagcg 
        tttaatctg caggataat cgatttgtg aagttgttg 
        aagcgaatg gcgcccagt tcgttcggg aaacaaatg 
        gataaggtt gggaggtat aacaataaa agacttagg 
        tgcactgcg attccttgt aaaaagaaa aatccgccg 
        aagaagaag gcaggcacc cagaagaaa ccctaattg 
        ccaccaaag tttttaatt taggagcta acttattta 
        ttta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY