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Gene information
General information
TC004297 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451971.1: 37189..46903
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004297
    iBeetle screen iB_03721

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (41 dpi)
    Days post injection


    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004297 RA
      MGTRAVLVL LIFLSVGRS DRSLRSVFG AIFVPGTQF 
      SQSENSVSQ SAPPEAPPM AYSVTEKEF TALRIEYIK 
      NQILKKLRL KEKPTVALA ELPKPVKEY DNLIPDQED 
      NLHSGYSED FYGKTTQAI IFPYEDEAR CLRKIRFPS 
      VCVPFQMPT DIHSTDVST AELWFHKVP DNLDSHNQT 
      FVVSEVAHW DTNKSFQKT KPIAIQETS LTEGWLKVD 
      VTYVVKNWL DFQDSPTHA ISVACKTCG MDKNQSPIS 
      FSSEFKPFL VVYTHSQQR RSLHRRPKR ATKCSAGRN 
      ECCRESFYV SFADIGWDD WIMSPAGYD AYFCKGSCV 
      TASALTLSA TQHNSILQK VMYGQNKNR GSRPELTPC 
      CAATQFQPL QLVYMDNNK TMMTKVLSN MIVESCGCW
    CDS sequence
    • >TC004297 RA
      atgggcacc agagccgtt ttggtgttg ctgattttc 
      ctctcggtc ggtcgcagt gatcgaagt ttgcggtcg 
      gtttttggc gcaattttc gttccgggc acgcagttc 
      agccaatcg gagaacagt gtgagccag agcgcgccc 
      ccagaagcc cccccgatg gcctattcg gtcaccgag 
      aaggagttc accgcgttg cggatcgaa tacatcaaa 
      aaccaaatc ctgaagaag ctcaggctg aaggagaag 
      cccacggtg gctttggcc gagctgccc aagccagtc 
      aaggagtac gacaatttg ataccggac caggaggat 
      aatttgcac agtggatac tcggaggac ttctacggg 
      aagaccacg caagccatc atcttcccc tatgaagat 
      gaggctcga tgtttgcga aaaattcgc ttcccctca 
      gtttgcgtc cctttccaa atgcccact gatatccac 
      tcgaccgat gttagcacg gctgaactt tggtttcac 
      aaagtgcct gataatctg gactcccat aatcaaact 
      ttcgtggta tcggaagtt gcccattgg gacaccaat 
      aagagtttc caaaaaacg aagcctata gccatccag 
      gaaacgagt ctcacagag ggttggctg aaggtggac 
      gtgacgtac gtggtgaaa aactggctc gatttccaa 
      gactcgcca acgcacgcc atcagcgtg gcttgcaag 
      acctgcggc atggacaag aaccagtct cccatttcg 
      ttttcgagc gaatttaag ccgtttttg gtggtctac 
      acccactcg cagcagagg aggtcgctg caccgccgg 
      ccgaagagg gcaacgaag tgctccgca ggccggaac 
      gagtgctgc agggagagc ttctacgtc tcgtttgcg 
      gacatcggc tgggacgac tggatcatg agtcctgca 
      ggatacgac gcctacttc tgcaaaggc tcgtgtgtc 
      acagcctcc gcgctgacg ctcagcgcc acgcaacat 
      aacagtata ttacagaaa gtcatgtac gggcagaat 
      aaaaatcgc ggctcgcga cccgaattg actccctgt 
      tgcgctgca acgcagttt cagccgctg cagctggtt 
      tatatggac aataataaa actatgatg acgaaagtt 
      ctgtcgaat atgatagtg gaaagttgt ggttgttgg 
      tga
    mRNA sequence
    • >TC004297 RA
      tccaacaga atgagagtc acaatagaa cgtcttata 
      aaactcgta taataatgt gacgtacca agatgtatt 
      gaacaattg atttcattt gatctgttt aatgttgca 
      ctaatgtta aataaagca aatgtttct taaaaatat 
      atattagaa attatacaa tacaaaaaa aaacaatct 
      taaacagga aataaacaa cttcgcaaa ataattatt 
      aatttttaa tgaaaaaat aaatctaac caacaacta 
      gataaaatt aggaaatgt ttacacgga aaaagacaa 
      acaaaatgg aactatcat gtcttgaag cgccaatgg 
      atttaaatc cggtagaca ataaaaaca aaattgaat 
      ctcaatttt ttaataggt gatttataa actcaatat 
      cagtgtcgt agaattcgt tgcaaaagt gaccgggct 
      tgttattta gttgaaaac ttagtccag caaaaagtt 
      tgttttatc ctgtatggc taggtggtg aactggtta 
      gcttgcgat tgagtgaat tttcggggc ttgcttcca 
      agtgccaag ttcgacatt ggggttgtg gttgtggct 
      ccaagccgt tcagatttt cattactta aaacttgag 
      cggaagtac gtccagttc atattctcg tattatttc 
      taaataagt gatatatca gtagcttgt taacgcaat 
      ctactctct ataagcaga ttagtttct tcgctgtta 
      catgtttta ttttgaatg atgaaattt taatatttg 
      actaggaat acttacaag ttttataca acattgtat 
      aggattttc aaagtgata gctatacat atatatcgg 
      ttacgttgg tgtgctgtg cttacggat cagttaggt 
      tattgaagg tgttatatt tagtactac gcgatcatt 
      tctattact gctaggagc gttttccgc aagaatcaa 
      acatgttca tattacctt tgttcttta ataataaac 
      atgcaaatg tgatatggt ttttaattc tactaattt 
      tgtattgct ttgtttacg ttttacaca tattttaac 
      aatcctacc aacaataaa ttgtacagt ttctattct 
      gagtgttga tacaatttc atacaaaat aaatatcta 
      ataataaca ttacgaaac atgactcgt ttttctgcc 
      cttccgaat ccctcaggt cattcacac aattcgtgt 
      ttatgtgtt aattaatga ctaatactg aaaacatcc 
      gaagatatt ttttaattt gattagttg tggagaaat 
      aaacaatat tttatagta cggcatttg attatgaat 
      ttgttttgc tcccgctat ctgattcaa accgaagga 
      aagttacga gataaaaaa ctgataaac cacactact 
      accgctgat tttgcttct agaaatagt ttttcattg 
      ataaaaaac aagcgtgtg cgccgttac gtttggaca 
      tgcggatac gccttggag gccacctca tgaagtact 
      taacatttt ctgaaagct gttccttcc tacgtgaca 
      tagcacgtg ctgaaacca ccgaatcgg tatcatcta 
      aatttgaca taacggcta ctctagttc tgatagaac 
      ctcaggttc aaggttcat ttctatcat ccgttacag 
      aaatgccaa gaactcgag agtggggaa ttaaatcgc 
      tgtgtttta agagtgcac ttctcggca ccaatctag 
      tttaaaatc atctgtaaa caacgcaaa atgggcacc 
      agagccgtt ttggtgttg ctgattttc ctctcggtc 
      ggtcgcagt gatcgaagt ttgcggtcg gtttttggc 
      gcaattttc gttccgggc acgcagttc agccaatcg 
      gagaacagt gtgagccag agcgcgccc ccagaagcc 
      cccccgatg gcctattcg gtcaccgag aaggagttc 
      accgcgttg cggatcgaa tacatcaaa aaccaaatc 
      ctgaagaag ctcaggctg aaggagaag cccacggtg 
      gctttggcc gagctgccc aagccagtc aaggagtac 
      gacaatttg ataccggac caggaggat aatttgcac 
      agtggatac tcggaggac ttctacggg aagaccacg 
      caagccatc atcttcccc tatgaagat gaggctcga 
      tgtttgcga aaaattcgc ttcccctca gtttgcgtc 
      cctttccaa atgcccact gatatccac tcgaccgat 
      gttagcacg gctgaactt tggtttcac aaagtgcct 
      gataatctg gactcccat aatcaaact ttcgtggta 
      tcggaagtt gcccattgg gacaccaat aagagtttc 
      caaaaaacg aagcctata gccatccag gaaacgagt 
      ctcacagag ggttggctg aaggtggac gtgacgtac 
      gtggtgaaa aactggctc gatttccaa gactcgcca 
      acgcacgcc atcagcgtg gcttgcaag acctgcggc 
      atggacaag aaccagtct cccatttcg ttttcgagc 
      gaatttaag ccgtttttg gtggtctac acccactcg 
      cagcagagg aggtcgctg caccgccgg ccgaagagg 
      gcaacgaag tgctccgca ggccggaac gagtgctgc 
      agggagagc ttctacgtc tcgtttgcg gacatcggc 
      tgggacgac tggatcatg agtcctgca ggatacgac 
      gcctacttc tgcaaaggc tcgtgtgtc acagcctcc 
      gcgctgacg ctcagcgcc acgcaacat aacagtata 
      ttacagaaa gtcatgtac gggcagaat aaaaatcgc 
      ggctcgcga cccgaattg actccctgt tgcgctgca 
      acgcagttt cagccgctg cagctggtt tatatggac 
      aataataaa actatgatg acgaaagtt ctgtcgaat 
      atgatagtg gaaagttgt ggttgttgg tgatgacac 
      tgtaacagt aacagtatt ttgtttcgt gatttttca 
      cgtgcaact aaccaaagc gagagatac caaaaattg 
      gggaaattt tcgttttag aatttttcg ccaggcgag 
      gattttcta cgtcaattt aatttattt cttagttaa 
      ttttatgtc aagctatgt tatatttat tttatgtgt 
      attttttaa ttttataag taaaccaat tgagcaaat 
      atgcgtgtt tttatcctt cctcttcca aataattcc 
      aaataaaca gtcctgaaa tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY