Status
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Gene information
General information
TC004261 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451856.1: 30..6740
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004261
    iBeetle screen iB_03717

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004261 RA
      MKAAAPTAP DSTITKLLS CAFAISSKE SAALGFPDF 
      PLDNLTLGA KILENNPKL PPYDLFFRL YPYKLFLKE 
      QTTHLESLL ESFDVKPQP QSVFSAFTQ KVKTFLATP 
      TPKKTYIET DYQNKLIEE MLQSEQTAD FCIIGPRGS 
      GKSLLVQQV ANIAGKQTE NIVLYQDMT ARDLIQQRT 
      TLENGDTVW TLSPLVLAA LEGKIAVLD GIHRIHPST 
      LSCLHRLVQ NRELQLHDG KRLISHERY QLLKTKTTP 
      TDGVLPIHP DFRIVAIGE PPNTNTTNW LTPEVLSLF 
      LFHEVRSLT KQEELHVVT SKYGPVSPQ MHKIIELAH 
      VLRHSGDQT LLNLAGHLS TRQILRLAA RMSQFPTTD 
      LYDVLETTF MVKFLPSLA RGALERTFE RLGVTQAAR 
      DPISVKCEV QDGVLTIGA SSVPVHQTS NLSKVPEIL 
      FYDVPQHLQ LMERLLQDF HLGQHILLV GNQGVGKNK 
      IIDRFLQLM NRPREYIQL HRDTTVQTL TVQPTVKDG 
      VLVHEDSPL VKAVKSGHV LVVDEADKA PTHVTCILK 
      TLIESGQMV LSDGRRIGG TSPHHIPIH PDFRVVVLA 
      NRPGFPFLG NDFFGALGD LFSSHSVEN PEPEAEIAL 
      LRQYGPEVP LETIKKLVK VFGELRNMA DQGLVSYPY 
      STREVVNIV KHLNQFPNS DLEEVIFNV FDFDRYSSE 
      VVDTLGEIL EKHGFTGGK NLMLTPKKV KERIQVTVN 
      RKSGLDVSA PKHGKEDPK NEPHVGGNT WAGGTGGRD 
      TAGLGGKGG PYRLDKGHK VHQLSDEEK NSVPEHVQR 
      AAREMGRKA FQEKLKEIG MSGYDASLY NQFYHSVER 
      QVQSLRVIL GNLQAKNKE RHWTRHQTA GELDDVKLI 
      DGLLGEKTI FRRRAEQEP ELGAPQIKP KLFRLVVDV 
      SGSMYRFNG YDGRLDREL EAAVLVMEA FQGFDDKIQ 
      YDIIGHSGE APSIPFVKF KEPPKNNKE RLDIIRTMH 
      AHAQYCWSG DHTLEGAES AINSLAEED CDEGIVIVL 
      SDANLERYR IPPSKLAQV LTSKPNISA YAIFIGGLG 
      DQAERLTEK LPAGRAFIC RDVTQLPQI LKQIFSSSV 
      INI
    CDS sequence
    • >TC004261 RA
      atgaaagct gccgccccc accgccccc gactccaca 
      atcaccaaa ctactctca tgcgccttc gccatctca 
      tcaaaagaa agcgccgct ctgggattc ccggacttc 
      cccctcgac aatctaacc ctaggtgca aaaatccta 
      gaaaacaac cccaaacta ccaccctac gacctcttt 
      ttccgcctc tacccctac aaactcttc ctgaaagag 
      caaaccacc cacttagag tcactcctt gaatcgttc 
      gacgtcaaa ccccaaccc caatcagta ttctccgca 
      ttcacccaa aaagtgaaa acattcctc gcaacccca 
      accccaaaa aaaacctac atcgaaacc gactaccaa 
      aacaaacta atcgaggaa atgctccaa agcgaacaa 
      accgcagat ttctgcatt atcggcccg cgcggctcc 
      ggtaaatcc ctgctagtg caacaagtt gccaacatt 
      gcaggcaaa caaaccgaa aacatagtc ctgtaccag 
      gacatgaca gctagggac ttgatccaa cagcgcaca 
      acactggaa aatggcgac actgtctgg accctgtcc 
      ccgttagtg ttggcagca ctggagggg aaaatcgcg 
      gtgttggac ggaatccac cgaattcac ccgagcaca 
      ctgtcgtgc ctccaccgc ctggtccag aaccgcgaa 
      ctgcagctg cacgacggc aaacgactc atttcccac 
      gagcgctac caacttctc aaaaccaaa accacccca 
      accgacggg gttttaccc attcacccc gatttccgc 
      attgtcgca atcggggaa cccccaaac accaacacc 
      accaattgg ctaacgcct gaagttctc agcctgttt 
      ctcttccac gaagtgcgt tccttaacc aaacaagag 
      gaactccac gttgttacg tcaaaatac ggcccggtt 
      tcgccccaa atgcacaaa atcatcgag ttggcacac 
      gtgttacgt cacagtggc gaccaaact ctcctaaat 
      ctagcaggc catctgtca acgcgtcaa atattgaga 
      ctagcagcc cgaatgtca caattcccg actactgat 
      ctctatgat gtccttgaa accactttc atggtcaag 
      tttttgccg tctttagcg cggggggct ttggaacgt 
      acttttgag aggttgggt gtgacgcaa gcggcacgt 
      gacccgatt agtgtcaaa tgtgaggtg caagatggc 
      gtcttgacg attggcgcg tcatctgtg cccgtccac 
      caaacgtcg aatttgagt aaagtcccc gaaatttta 
      ttctatgac gtgccgcaa catttacaa cttatggaa 
      cgtttattg caagatttt catttaggg caacatatt 
      ctcctagtg gggaatcaa ggtgtgggg aaaaataaa 
      ataattgat aggtttttg caattgatg aatcgccct 
      cgtgagtac atccagctg cacagggac accaccgtc 
      caaacattg acagtgcag cccactgtc aaagatggc 
      gttttagtt catgaagac tcgccactt gtcaaggct 
      gtcaaaagc ggccatgtt ttggtagtg gatgaggca 
      gacaaggcc cccacccat gtcacatgt attctaaag 
      actctgatc gaatcaggt cagatggtc ctaagcgat 
      ggacgtagg attggtggc acttccccc caccacatc 
      cccatccat cccgacttt agggtggta gtactggcg 
      aacaggccc ggcttcccg ttccttggc aacgatttc 
      ttcggggct ttgggcgac ttgttcagc agccattcg 
      gtggaaaat cccgaaccg gaggccgaa atcgccctt 
      ttgcgccag tacggccct gaagtcccc ctcgaaact 
      atcaaaaaa cttgtcaaa gttttcggg gaattacgc 
      aatatggcc gaccagggt ctagtgtcg tatccgtac 
      tccacgcgt gaggttgta aacattgtc aaacacttg 
      aatcaattc cccaattct gaccttgag gaggtcata 
      tttaacgtg ttcgacttt gatcggtac tcgtcggaa 
      gtggtcgat accttgggg gaaatactg gagaagcat 
      gggttcaca ggggggaaa aacttgatg ttgacaccg 
      aaaaaagtg aaggagagg attcaggtc actgtgaat 
      aggaaaagt gggctcgat gtgagcgcc cccaagcat 
      gggaaggag gatccgaag aatgagccc cacgttggg 
      gggaatacc tgggcgggg ggcaccggg gggcgggac 
      acggccggg ttggggggc aaagggggg ccctatagg 
      ctcgataag gggcacaag gtacaccaa ctctccgac 
      gaggaaaaa aactcagtc ccggagcac gtgcaaagg 
      gcggctcgt gaaatgggg cgcaaggcg ttccaggaa 
      aaattaaaa gaaatcgga atgagtggt tacgacgcc 
      tcgctttac aaccaattc taccattct gtcgaacgt 
      caagtccag agtttgcgc gtgattttg gggaattta 
      caggcgaaa aacaaggaa cgccactgg acacgtcac 
      caaacggcg ggcgaattg gacgatgtc aaactaatc 
      gatggatta ttgggcgaa aaaacgatt tttcgcaga 
      cgtgctgag caggagccc gaacttggg gccccgcaa 
      attaagccc aaactgttc cggttggtt gtggacgtc 
      tctgggagt atgtatagg ttcaatggt tacgacggc 
      cgacttgac cgcgagctc gaagccgcc gttttggta 
      atggaggcg tttcaaggc ttcgacgat aaaatccaa 
      tacgacatt atcggccat tcgggcgaa gccccgtct 
      atacccttc gtcaagttt aaagaaccg ccaaaaaac 
      aacaaagaa cgcttggat ataatccga acaatgcac 
      gcgcatgcg cagtattgt tggtccggt gatcacacg 
      ctagagggc gccgaatcg gctattaat tcactagcg 
      gaagaggat tgtgacgag ggaattgtt attgttctg 
      tctgatgct aatttagaa cgttacaga ataccaccg 
      tcaaagttg gcacaggtt ttgacctcc aaaccaaat 
      atcagtgcc tatgcgatt tttatcgga ggattgggg 
      gatcaagcc gagagatta accgaaaaa ttaccagcc 
      ggacgtgct tttatttgt cgtgacgtc acacaattg 
      ccgcaaatt ttgaaacag attttttcg tcgtctgtc 
      atcaatata tga
    mRNA sequence
    • >TC004261 RA
      tcccacata aatcgaatt ttttttcgc aaagttcca 
      attaatatt tttgaacga aattttggc agacgtaga 
      aatcacttt tccgtcagg aatgctacc aagagatga 
      aagctgccg cccccaccg cccccgact ccacaatca 
      ccaaactac tctcatgcg ccttcgcca tctcatcaa 
      aagaaagcg ccgctctgg gattcccgg acttccccc 
      tcgacaatc taaccctag gtgcaaaaa tcctagaaa 
      acaacccca aactaccac cctacgacc tctttttcc 
      gcctctacc cctacaaac tcttcctga aagagcaaa 
      ccacccact tagagtcac tccttgaat cgttcgacg 
      tcaaacccc aaccccaat cagtattct ccgcattca 
      cccaaaaag tgaaaacat tcctcgcaa ccccaaccc 
      caaaaaaaa cctacatcg aaaccgact accaaaaca 
      aactaatcg aggaaatgc tccaaagcg aacaaaccg 
      cagatttct gcattatcg gcccgcgcg gctccggta 
      aatccctgc tagtgcaac aagttgcca acattgcag 
      gcaaacaaa ccgaaaaca tagtcctgt accaggaca 
      tgacagcta gggacttga tccaacagc gcacaacac 
      tggaaaatg gcgacactg tctggaccc tgtccccgt 
      tagtgttgg cagcactgg aggggaaaa tcgcggtgt 
      tggacggaa tccaccgaa ttcacccga gcacactgt 
      cgtgcctcc accgcctgg tccagaacc gcgaactgc 
      agctgcacg acggcaaac gactcattt cccacgagc 
      gctaccaac ttctcaaaa ccaaaacca ccccaaccg 
      acggggttt tacccattc accccgatt tccgcattg 
      tcgcaatcg gggaacccc caaacacca acaccacca 
      attggctaa cgcctgaag ttctcagcc tgtttctct 
      tccacgaag tgcgttcct taaccaaac aagaggaac 
      tccacgttg ttacgtcaa aatacggcc cggtttcgc 
      cccaaatgc acaaaatca tcgagttgg cacacgtgt 
      tacgtcaca gtggcgacc aaactctcc taaatctag 
      caggccatc tgtcaacgc gtcaaatat tgagactag 
      cagcccgaa tgtcacaat tcccgacta ctgatctct 
      atgatgtcc ttgaaacca ctttcatgg tcaagtttt 
      tgccgtctt tagcgcggg gggctttgg aacgtactt 
      ttgagaggt tgggtgtga cgcaagcgg cacgtgacc 
      cgattagtg tcaaatgtg aggtgcaag atggcgtct 
      tgacgattg gcgcgtcat ctgtgcccg tccaccaaa 
      cgtcgaatt tgagtaaag tccccgaaa ttttattct 
      atgacgtgc cgcaacatt tacaactta tggaacgtt 
      tattgcaag attttcatt tagggcaac atattctcc 
      tagtgggga atcaaggtg tggggaaaa ataaaataa 
      ttgataggt ttttgcaat tgatgaatc gccctcgtg 
      agtacatcc agctgcaca gggacacca ccgtccaaa 
      cattgacag tgcagccca ctgtcaaag atggcgttt 
      tagttcatg aagactcgc cacttgtca aggctgtca 
      aaagcggcc atgttttgg tagtggatg aggcagaca 
      aggccccca cccatgtca catgtattc taaagactc 
      tgatcgaat caggtcaga tggtcctaa gcgatggac 
      gtaggattg gtggcactt ccccccacc acatcccca 
      tccatcccg actttaggg tggtagtac tggcgaaca 
      ggcccggct tcccgttcc ttggcaacg atttcttcg 
      gggctttgg gcgacttgt tcagcagcc attcggtgg 
      aaaatcccg aaccggagg ccgaaatcg cccttttgc 
      gccagtacg gccctgaag tccccctcg aaactatca 
      aaaaacttg tcaaagttt tcggggaat tacgcaata 
      tggccgacc agggtctag tgtcgtatc cgtactcca 
      cgcgtgagg ttgtaaaca ttgtcaaac acttgaatc 
      aattcccca attctgacc ttgaggagg tcatattta 
      acgtgttcg actttgatc ggtactcgt cggaagtgg 
      tcgatacct tgggggaaa tactggaga agcatgggt 
      tcacagggg ggaaaaact tgatgttga caccgaaaa 
      aagtgaagg agaggattc aggtcactg tgaatagga 
      aaagtgggc tcgatgtga gcgccccca agcatggga 
      aggaggatc cgaagaatg agccccacg ttgggggga 
      atacctggg cggggggca ccggggggc gggacacgg 
      ccgggttgg ggggcaaag gggggccct ataggctcg 
      ataaggggc acaaggtac accaactct ccgacgagg 
      aaaaaaact cagtcccgg agcacgtgc aaagggcgg 
      ctcgtgaaa tggggcgca aggcgttcc aggaaaaat 
      taaaagaaa tcggaatga gtggttacg acgcctcgc 
      tttacaacc aattctacc attctgtcg aacgtcaag 
      tccagagtt tgcgcgtga ttttgggga atttacagg 
      cgaaaaaca aggaacgcc actggacac gtcaccaaa 
      cggcgggcg aattggacg atgtcaaac taatcgatg 
      gattattgg gcgaaaaaa cgatttttc gcagacgtg 
      ctgagcagg agcccgaac ttggggccc cgcaaatta 
      agcccaaac tgttccggt tggttgtgg acgtctctg 
      ggagtatgt ataggttca atggttacg acggccgac 
      ttgaccgcg agctcgaag ccgccgttt tggtaatgg 
      aggcgtttc aaggcttcg acgataaaa tccaatacg 
      acattatcg gccattcgg gcgaagccc cgtctatac 
      ccttcgtca agtttaaag aaccgccaa aaaacaaca 
      aagaacgct tggatataa tccgaacaa tgcacgcgc 
      atgcgcagt attgttggt ccggtgatc acacgctag 
      agggcgccg aatcggcta ttaattcac tagcggaag 
      aggattgtg acgagggaa ttgttattg ttctgtctg 
      atgctaatt tagaacgtt acagaatac caccgtcaa 
      agttggcac aggttttga cctccaaac caaatatca 
      gtgcctatg cgattttta tcggaggat tgggggatc 
      aagccgaga gattaaccg aaaaattac cagccggac 
      gtgctttta tttgtcgtg acgtcacac aattgccgc 
      aaattttga aacagattt tttcgtcgt ctgtcatca 
      atatatgag gggagatta ggcacgaat taattaatt 
      gtaaataaa aagtaatat atttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY