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Gene information
General information
TC004246 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015452061.1: 4345..40153
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004246
    iBeetle screen iB_03716

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    urogomphi not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    urogomphi number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    urogomphi orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    pygopodium shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004246 RA
      MDPPQKHEK IKLVEINSY LTCYLCKGY LIDATTISE 
      CLHSFCRSC IIKFLQKNC YCPVCEVII NKAKPCLKL 
      DKTLQDIVY KLVPGLFVN EMYRRKEFY QNRPGLAPK 
      VSPEERGED TERTIFNPQ DAISLSIEY ISDDSAPGA 
      IPSLPTNTE QPHDNPMKR YLQCPGMCR IEVLKKFVR 
      NKYNVDTNL FHIDILYKR VPLPDHYTL IDIGYIYSW 
      KRNEPMKFF FRITDINKV TERFDYFDG RTTPEVVHK 
      PLKVKEKKP THVKKEKCF KPIKVSSPK PVKSEVKCF 
      KPVASPKPV KCFKPVKSE LGASPKPVK TEFGASPKP 
      VKCEVASPK PVKCEVTSP KPVRCFKPV ASPKPVKSE 
      PELKTGKSD EVRLKPEIT EEIKTKIQN GDSVYTNIT 
      LNRDDNVEI ITKIQKVSN KEGHPIGLN IINHTVKKV 
      QNDKLVPSV DAKTEQENK QKEDFLKSI ELTAKSSLE 
      KKETKALPP QLPPAPLPV TPSSSSQKR KNSSPMKND 
      KKPKIEKKP MKIIIQPKS IQNPTVRQM VTNVNQNVN 
      NMSKIVEQT NKQTDLQSL INNCKINIP ASLSITLNE 
      GADGGARTP PSIPPVKNY IEILKIEDK DKKQTPTFQ 
      KIFEESIKS KSPPSADKP APLDLTSDN STTPKRKIL 
      EIASQLSKK TKVEPQPES KCEDVGGKI AIPRLATQR 
      TLKPPSKTS ASMHSPSLG LNYTVSVGQ AKGGKGAPS 
      TSKALSPNH LLEKYNIQN LAQLTASFN FNGGFGALQ 
      QAMLLKQME HHSWLNQGP LLQYEKYLQ SLSAQSKLM 
      GNIKEN
    CDS sequence
    • >TC004246 RA
      atggatcct cctcaaaag cacgagaag atcaaactt 
      gtcgagata aactcatac ttgacctgt tatctctgc 
      aaggggtat ctgatcgac gccaccacc atatcggaa 
      tgtctgcat tcgttttgc cgcagttgc ataatcaag 
      ttcctccaa aagaactgc tactgtccg gtgtgcgaa 
      gtcatcatc aacaaggcc aagccttgt ctcaaattg 
      gataaaact ctacaagac attgtttat aaactggtt 
      ccgggcctt ttcgtcaac gaaatgtac cgacgtaaa 
      gaattttat caaaacagg cccggacta gcaccgaaa 
      gtgtccccc gaggagcgt ggcgaagac accgaacga 
      acaattttc aatcctcaa gacgccata agtctttca 
      atcgaatat atcagtgat gattcggcc ccaggtgcc 
      ataccatca ctaccaacc aacaccgaa caaccccac 
      gacaacccc atgaagaga tatcttcaa tgtccgggg 
      atgtgtcgt atcgaagtt ttgaagaaa ttcgtcaga 
      aataaatac aatgtcgat acgaatctc tttcatatc 
      gatatttta tataagagg gtgccctta cctgaccat 
      tacaccctc atcgatatc ggttacatc tattcgtgg 
      aaacgaaac gaaccgatg aaattcttc ttccgtatc 
      actgatatc aacaaagtg accgaacgc ttcgactat 
      ttcgacggc cggacgacg ccggaagtc gtccataaa 
      ccactcaag gtcaaggag aagaagccc actcacgtg 
      aagaaagag aagtgcttc aaaccaatc aaagtgtcg 
      agtccaaaa ccggttaaa agtgaagtg aagtgtttc 
      aaaccagtg gcgagtcca aaaccggtc aagtgtttc 
      aaaccggtt aagagtgaa cttggcgcg agtccaaaa 
      ccggttaag actgaattt ggcgcgagt ccaaaaccg 
      gtcaagtgt gaagtggcg agtccaaaa ccggttaag 
      tgtgaagtg acgagtcca aaaccggtt aggtgtttc 
      aaaccagtg gcgagtcca aaaccggtt aagtctgaa 
      ccggaactg aaaactggc aaaagtgac gaagtgcgt 
      ttaaaaccg gaaataacc gaagaaata aaaacgaaa 
      attcaaaac ggtgactcg gtctatacg aacataacg 
      ttgaatcgt gacgataac gtggaaatt atcacaaaa 
      atacaaaaa gtgtcgaat aaagaaggc catcctatc 
      ggacttaat atcataaat catacggtg aagaaagta 
      caaaacgat aagttagtt cctagtgtt gacgcaaag 
      actgagcaa gaaaacaaa caaaaggaa gattttctc 
      aaatcgatc gaactgacg gcgaaaagt tcgctcgaa 
      aagaaagaa actaaagcc ctcccaccc cagctcccc 
      ccagccccc cttccggta acgccttcg tcctcctcc 
      caaaaacgg aagaattca agtccaatg aaaaacgac 
      aagaaaccg aaaatcgag aaaaaaccc atgaaaatc 
      atcatccaa ccaaaatcc atccaaaac ccgaccgtg 
      cgccaaatg gtgacaaac gtcaaccaa aacgtcaac 
      aacatgtca aaaatcgtg gaacaaacg aacaaacaa 
      accgacctc caatccctc atcaacaac tgcaaaatc 
      aacataccg gcctccctc tccataacc ttgaacgag 
      ggggccgac gggggcgcc cgcacgccc ccctccatc 
      ccccccgtg aaaaactac atcgaaatc ctgaaaatt 
      gaagacaag gacaagaaa caaaccccg acatttcag 
      aaaatcttc gaggaaagc atcaaaagt aaatcgccg 
      ccttcggcg gacaaaccc gcccccctt gacctaacc 
      agcgacaac tccaccacc cccaagcgc aagatcctc 
      gagatcgcc tcccagctg tcgaagaag accaaagtt 
      gagccccag ccagagagc aagtgcgag gatgtcggg 
      gggaaaatc gccattccg cggctggcg acgcagagg 
      actttgaag cccccgtcc aagaccagc gccagcatg 
      cactcgccc agtctgggc ttgaactac accgtctcc 
      gtggggcag gccaagggg ggcaagggg gcgccgtcg 
      acgtccaag gcgctcagt ccgaaccat ttgctcgag 
      aagtacaat atacagaat ctggcgcag ttgacggcc 
      agttttaat tttaacggg ggctttggg gcgctgcag 
      caggccatg ctgctgaag cagatggag catcatagt 
      tggctgaat caagggccg ctgttgcag tacgagaag 
      tatttgcag agtttgagt gcgcagagc aagctgatg 
      gggaatatc aaggagaat tga
    mRNA sequence
    • >TC004246 RA
      tacagaacg catcagtct gccgagcga gatcgtaga 
      gtgctgtga gtcgcattt cgccgtgtg aaaaaccgc 
      cgccgccgc cgtctccgt cgaccagcc gcctccagg 
      ccctcgtcc ggcggcccc acgccgccc ccaggtgtc 
      ccgatcggg taaaatcag cgaaaaagc gtgaaatgt 
      gtgcgtgtt gaggttagg tgtgtcgat aagggcgag 
      aatcgctca agccggccg tgcattgct tttgttttc 
      acggtatta cttaactta aatcggggt gcatcgagt 
      ggcctgtgg aaatgagta gtgacgttt tgcgcaaga 
      ggaaaagtg gagttttcg cacggtttg ggggaaatt 
      aggttaaac agcgatgga tcctcctca aaagcacga 
      gaagatcaa acttgtcga gataaactc atacttgac 
      ctgttatct ctgcaaggg gtatctgat cgacgccac 
      caccatatc ggaatgtct gcattcgtt ttgccgcag 
      ttgcataat caagttcct ccaaaagaa ctgctactg 
      tccggtgtg cgaagtcat catcaacaa ggccaagcc 
      ttgtctcaa attggataa aactctaca agacattgt 
      ttataaact ggttccggg ccttttcgt caacgaaat 
      gtaccgacg taaagaatt ttatcaaaa caggcccgg 
      actagcacc gaaagtgtc ccccgagga gcgtggcga 
      agacaccga acgaacaat tttcaatcc tcaagacgc 
      cataagtct ttcaatcga atatatcag tgatgattc 
      ggccccagg tgccatacc atcactacc aaccaacac 
      cgaacaacc ccacgacaa ccccatgaa gagatatct 
      tcaatgtcc ggggatgtg tcgtatcga agttttgaa 
      gaaattcgt cagaaataa atacaatgt cgatacgaa 
      tctctttca tatcgatat tttatataa gagggtgcc 
      cttacctga ccattacac cctcatcga tatcggtta 
      catctattc gtggaaacg aaacgaacc gatgaaatt 
      cttcttccg tatcactga tatcaacaa agtgaccga 
      acgcttcga ctatttcga cggccggac gacgccgga 
      agtcgtcca taaaccact caaggtcaa ggagaagaa 
      gcccactca cgtgaagaa agagaagtg cttcaaacc 
      aatcaaagt gtcgagtcc aaaaccggt taaaagtga 
      agtgaagtg tttcaaacc agtggcgag tccaaaacc 
      ggtcaagtg tttcaaacc ggttaagag tgaacttgg 
      cgcgagtcc aaaaccggt taagactga atttggcgc 
      gagtccaaa accggtcaa gtgtgaagt ggcgagtcc 
      aaaaccggt taagtgtga agtgacgag tccaaaacc 
      ggttaggtg tttcaaacc agtggcgag tccaaaacc 
      ggttaagtc tgaaccgga actgaaaac tggcaaaag 
      tgacgaagt gcgtttaaa accggaaat aaccgaaga 
      aataaaaac gaaaattca aaacggtga ctcggtcta 
      tacgaacat aacgttgaa tcgtgacga taacgtgga 
      aattatcac aaaaataca aaaagtgtc gaataaaga 
      aggccatcc tatcggact taatatcat aaatcatac 
      ggtgaagaa agtacaaaa cgataagtt agttcctag 
      tgttgacgc aaagactga gcaagaaaa caaacaaaa 
      ggaagattt tctcaaatc gatcgaact gacggcgaa 
      aagttcgct cgaaaagaa agaaactaa agccctccc 
      accccagct ccccccagc cccccttcc ggtaacgcc 
      ttcgtcctc ctcccaaaa acggaagaa ttcaagtcc 
      aatgaaaaa cgacaagaa accgaaaat cgagaaaaa 
      acccatgaa aatcatcat ccaaccaaa atccatcca 
      aaacccgac cgtgcgcca aatggtgac aaacgtcaa 
      ccaaaacgt caacaacat gtcaaaaat cgtggaaca 
      aacgaacaa acaaaccga cctccaatc cctcatcaa 
      caactgcaa aatcaacat accggcctc cctctccat 
      aaccttgaa cgagggggc cgacggggg cgcccgcac 
      gcccccctc catcccccc cgtgaaaaa ctacatcga 
      aatcctgaa aattgaaga caaggacaa gaaacaaac 
      cccgacatt tcagaaaat cttcgagga aagcatcaa 
      aagtaaatc gccgccttc ggcggacaa acccgcccc 
      ccttgacct aaccagcga caactccac cacccccaa 
      gcgcaagat cctcgagat cgcctccca gctgtcgaa 
      gaagaccaa agttgagcc ccagccaga gagcaagtg 
      cgaggatgt cggggggaa aatcgccat tccgcggct 
      ggcgacgca gaggacttt gaagccccc gtccaagac 
      cagcgccag catgcactc gcccagtct gggcttgaa 
      ctacaccgt ctccgtggg gcaggccaa ggggggcaa 
      gggggcgcc gtcgacgtc caaggcgct cagtccgaa 
      ccatttgct cgagaagta caatataca gaatctggc 
      gcagttgac ggccagttt taattttaa cgggggctt 
      tggggcgct gcagcaggc catgctgct gaagcagat 
      ggagcatca tagttggct gaatcaagg gccgctgtt 
      gcagtacga gaagtattt gcagagttt gagtgcgca 
      gagcaagct gatggggaa tatcaagga gaattgacg 
      gcgggttgt tggattttg ttagtttga gttttggca 
      acttcgtta atgttgtgt cctattgtt attttcgac 
      atttttaaa tcagttatt atgagaggt ttcattgta 
      ttataatgt atgtaaatt gtagaatta tagactcta 
      agggaaatg taaattatt ttacaattt tcattaaaa 
      aatttttga aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY