Status
Please wait ...
Gene information
General information
TC004245 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451620.1: 128571..139875
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004245
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03715

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004245 RA
      MTNSQVGFR GVRSSKFRH VYGEPAKRE KCYDNINIT 
      KNAHDSQFC AVNPKFVAL VTEVAGGGA FIVIPIEST 
      GRLDFNASR VTGHTGPVL DIKWNPFND NVIASCSDD 
      CTIKLWHIP DGGLSMHLT EWLVELQGH KRRVAYIEW 
      HPCAENILF SAGFDHLVI IWDIEKGET VNVIDCHPD 
      VIYSMSLNR DGSLLTTTC KDKKLRVIE PRSGIVKSE 
      GVCHAGSKA SKVTFLGSS GRLLTTGFS RHSDRQYSI 
      WDQNDLSKP LTIDTIDSS SGVVFPFFD CDSNIVFLA 
      GKGDGNIRY YEVTDEPPY IHFLSQFLS GSPQRGLGI 
      MPKRGLNIH ICEIFRFYK LHAIKGICE PISMIVPRK 
      SDQFHEDLY PDTAAPKPA LSAQEWIKG LNANPILMS 
      MKMGGNITT YKPRVYNNN KPGTTPIVA SQNNKMKLA 
      FLSTETIPD YRPKDLIEE KIKKTTTNQ TTKFQQLQQ 
      RFGHVEVNN DIPLIKEIN SMGDTITTE HDLKVAYNQ 
      QSEELRKVK KQLANSERR VKELEELVK KLQKK
    CDS sequence
    • >TC004245 RA
      atgacaaac tcacaggtg gggtttcga ggagttcgc 
      agctcgaaa ttccgtcac gtttacggc gagccagcg 
      aaacgagag aaatgttac gacaatata aacataaca 
      aaaaacgca cacgactct caattttgt gccgttaat 
      ccaaaattc gttgcttta gtcaccgaa gtggccggt 
      ggtggagct tttatagtc atacctatc gaaagtact 
      ggacgactg gattttaat gctagccga gtgacagga 
      catacagga ccggtttta gacataaaa tggaatcct 
      tttaatgac aacgtcatt gctagttgt tccgacgat 
      tgtactatt aaattatgg catattccg gacggtggt 
      ttatcaatg catttgacc gaatggttg gttgagctg 
      caagggcat aaacgaagg gtggcctac attgagtgg 
      catccttgt gctgaaaat atacttttc agtgccgga 
      ttcgaccat ttggtgatc atttgggac atagaaaaa 
      ggtgaaacc gtaaatgtg atcgactgt catccagac 
      gtgatttat tcaatgtca ctgaaccgc gatggcagt 
      cttctaaca acgacatgc aaagataaa aaattgcga 
      gtaattgaa ccgagaagt ggcatcgtt aaaagtgag 
      ggggtttgt catgctggt tccaaagcc agtaaagtg 
      acatttttg ggctcaagt gggcggtta ttaactacg 
      ggtttttca cgacattcc gatcgtcag tattcgatt 
      tgggatcaa aatgatttg agcaaaccc ttgactatt 
      gatacaatt gatagctcg tctggtgtt gtttttccc 
      ttttttgat tgtgattcc aatattgtg tttcttgct 
      ggcaagggc gatggcaat attaggtat tatgaggtt 
      acggacgaa ccgccttat atacatttt ctcagccag 
      tttctatcc ggaagtccc cagcgcggc ttaggaatc 
      atgcctaag cgcggctta aacatccac atttgcgaa 
      attttccgt ttttacaaa ctgcacgca atcaaaggt 
      atctgcgag ccaatcagt atgatcgtt ccgcgaaaa 
      agcgaccaa ttccatgaa gatttatac cctgatact 
      gccgctcca aagcctgcc ttatccgct caagaatgg 
      atcaaaggt ttgaatgca aatccgatt ttaatgtca 
      atgaaaatg ggtggaaat atcaccact tacaaacct 
      cgagtctac aataataat aaacctggc acaacacca 
      atcgttgct tcacaaaat aataaaatg aaattggct 
      tttctttca accgaaact attcccgat tatagacca 
      aaggatttg attgaggag aagattaag aaaacaacg 
      accaatcaa acgaccaaa tttcaacag ttgcaacaa 
      agatttgga catgtggag gtcaataat gatattcct 
      ttgattaag gaaatcaat agtatggga gatactatc 
      acgacggaa catgacctg aaagtggcg tacaatcag 
      cagtcggaa gaattgcgc aaagtgaaa aaacagctc 
      gccaacagc gaacgccgt gtcaaagag ctggaagaa 
      ctggtgaaa aaattacaa aagaagtaa
    mRNA sequence
    • >TC004245 RA
      ctgtcaaac aaatgtcaa aaaagttac gttgtgatt 
      tattgtttt attgaattt tttatttaa aatcagtac 
      aaaattgtt gggtttttt atgtaattc atttattat 
      gactggaga aacggaaag caatgttgg gaattctct 
      gttgccagc aaccggaga taaaataat taattttgt 
      ggataaccg ctcttgcag caaaccctc aaaaaggtc 
      ttctttatt gtggtggtg tcttgcatg aaacgctgc 
      caagagact gaatttgtt cttctgaag ctttttttt 
      tggatgtta cgatgacaa actcacagg tggggtttc 
      gaggagttc gcagctcga aattccgtc acgtttacg 
      gcgagccag cgaaacgag agaaatgtt acgacaata 
      taaacataa caaaaaacg cacacgact ctcaatttt 
      gtgccgtta atccaaaat tcgttgctt tagtcaccg 
      aagtggccg gtggtggag cttttatag tcataccta 
      tcgaaagta ctggacgac tggatttta atgctagcc 
      gagtgacag gacatacag gaccggttt tagacataa 
      aatggaatc cttttaatg acaacgtca ttgctagtt 
      gttccgacg attgtacta ttaaattat ggcatattc 
      cggacggtg gtttatcaa tgcatttga ccgaatggt 
      tggttgagc tgcaagggc ataaacgaa gggtggcct 
      acattgagt ggcatcctt gtgctgaaa atatacttt 
      tcagtgccg gattcgacc atttggtga tcatttggg 
      acatagaaa aaggtgaaa ccgtaaatg tgatcgact 
      gtcatccag acgtgattt attcaatgt cactgaacc 
      gcgatggca gtcttctaa caacgacat gcaaagata 
      aaaaattgc gagtaattg aaccgagaa gtggcatcg 
      ttaaaagtg agggggttt gtcatgctg gttccaaag 
      ccagtaaag tgacatttt tgggctcaa gtgggcggt 
      tattaacta cgggttttt cacgacatt ccgatcgtc 
      agtattcga tttgggatc aaaatgatt tgagcaaac 
      ccttgacta ttgatacaa ttgatagct cgtctggtg 
      ttgtttttc ccttttttg attgtgatt ccaatattg 
      tgtttcttg ctggcaagg gcgatggca atattaggt 
      attatgagg ttacggacg aaccgcctt atatacatt 
      ttctcagcc agtttctat ccggaagtc cccagcgcg 
      gcttaggaa tcatgccta agcgcggct taaacatcc 
      acatttgcg aaattttcc gtttttaca aactgcacg 
      caatcaaag gtatctgcg agccaatca gtatgatcg 
      ttccgcgaa aaagcgacc aattccatg aagatttat 
      accctgata ctgccgctc caaagcctg ccttatccg 
      ctcaagaat ggatcaaag gtttgaatg caaatccga 
      ttttaatgt caatgaaaa tgggtggaa atatcacca 
      cttacaaac ctcgagtct acaataata ataaacctg 
      gcacaacac caatcgttg cttcacaaa ataataaaa 
      tgaaattgg cttttcttt caaccgaaa ctattcccg 
      attatagac caaaggatt tgattgagg agaagatta 
      agaaaacaa cgaccaatc aaacgacca aatttcaac 
      agttgcaac aaagatttg gacatgtgg aggtcaata 
      atgatattc ctttgatta aggaaatca atagtatgg 
      gagatacta tcacgacgg aacatgacc tgaaagtgg 
      cgtacaatc agcagtcgg aagaattgc gcaaagtga 
      aaaaacagc tcgccaaca gcgaacgcc gtgtcaaag 
      agctggaag aactggtga aaaaattac aaaagaagt 
      aaatatcgt atgcacttt ttacgtatt gttaccaat 
      taagttgtg tttatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY