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Gene information
General information
TC004241 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451620.1: 8825..26109
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC004241
      iBeetle screen iB_03713

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC004241 RA
        MEPISNCTE AVTSVFVKL LQNETKNGH LDQFVKGLG 
        RMFYIIDPS DTTFKDQVP NFYRNAWPY FLLFILIEN 
        LVLYLDKKP TFRLNDGLT SLSHGLVQE CGRLIFRGA 
        ESYAYVYLY ENFRIVELR WDQASTWYL AAVGVDFCY 
        YWVHRACHE VHILWAQHQ VHHSSEEFN LAVGLRQSL 
        LQGWCGFIF YLPLAFAIP PTHFLTHQQ FNLLYQFWI 
        HTKAVKTLG PLEFFFNTP QHHRVHHGA NIWCLDKNY 
        GGVLIIWDR IFGTFAEER KNEEIVYGL VFNQPSFNI 
        LHLQTFYTA YVLQRFREM NCWRHKLAA IFYGPSWQP 
        GRPRLGVDE DKFKVVPRQ KYDVKLPLW CNIYLLVHF 
        CIVVYGFQE LASRHLVLN PVTVLAFVI YIIASLTSI 
        GLLFDNAPY ACIMELFRC MVLVTALQK LEFKDVDNG 
        VLVGCEALF VLSGLFWLL QSVKVLQIK EI
      CDS sequence
      • >TC004241 RA
        atggagccc atctccaat tgcaccgag gctgtgact 
        tcggttttc gtcaaactg ctgcaaaac gagaccaaa 
        aatgggcac ttggaccag tttgtcaag ggcttggga 
        cgcatgttc tacatcata gacccttcg gataccacg 
        ttcaaggac caggtgccg aacttttac agaaatgcg 
        tggccctac tttctcctc ttcattctg atcgaaaat 
        ctggtcctt tatctggat aagaagcca acgtttcgc 
        ctaaatgac ggcttgaca agtctatca cacggctta 
        gtgcaagaa tgcggaaga ctaatattc cggggcgct 
        gaaagctac gcttatgtg tatctttac gaaaacttc 
        cggattgtg gaattacgc tgggatcag gcgagtaca 
        tggtacttg gccgccgtc ggagtggat ttctgctat 
        tattgggtg catcgagct tgccatgag gtgcacatc 
        ctctgggcc cagcaccag gtccaccac agctcggag 
        gagttcaac ctcgctgta ggcctccgg caaagcctc 
        ctccaaggc tggtgcggc tttatattt tatttgcct 
        ctggctttc gccattcct ccgacgcat ttcctcacc 
        caccagcaa ttcaatttg ctttatcaa ttctggata 
        cataccaag gctgttaag acgctagga ccgttggaa 
        ttttttttt aatacgccg cagcatcat cgagttcat 
        cacggtgct aatatttgg tgcctcgat aaaaattac 
        ggcggggtt ttgattatt tgggaccgg atttttggg 
        acgtttgct gaagaaagg aaaaatgaa gagattgtc 
        tatggtttg gttttcaat caaccgtct ttcaatatt 
        ttacattta caaactttt tatacagcg tatgttttg 
        caacgattt cgggaaatg aactgttgg cggcataag 
        ttggctgcg attttttat gggccgagt tggcagcct 
        ggaaggccg agattagga gtagatgag gataagttc 
        aaagtagtt ccccggcag aaatacgac gtgaaattg 
        ccgctctgg tgtaatatc tacctcttg gtccacttc 
        tgcatcgtc gtttacggc tttcaagaa ttagcaagt 
        cgccatttg gtcctcaac cccgtcaca gtcctagcg 
        ttcgttatc tacatcatt gcatcgctc acgtcaata 
        ggactgttg ttcgacaat gcgccctac gcctgcatt 
        atggagctc ttccggtgc atggtcctc gttaccgcc 
        ctacaaaag ctggaattt aaggatgtg gataatggg 
        gtgttggtc ggctgtgag gcgcttttt gtcctctcg 
        ggactcttc tggctgctg cagagcgtc aaagttctg 
        caaatcaag gagatttag
      mRNA sequence
      • >TC004241 RA
        tttgtttga ataaacaaa aaataaaga aagaaaggt 
        ttaccaaag atttgaaaa tatattcag agaagagag 
        aaagactaa aaatacaat aaaaataaa cgtaaaaat 
        gagaaagag tgtttatta agtttaaaa caacaaaaa 
        atacaaaac gatgtaaaa attaaaaac acaaagcat 
        tagaaaaga aagaagtga cataacaca accagaaac 
        ctaaaaaat gttaaaaca aaaaaagaa aaaaaatag 
        aacttaaac ttaaaacct aatttaaaa aaaagggca 
        aataaaagt gctactccc tcccaccaa ttgtttaca 
        cactcgtcg tataaaacc cagcttttc gcactcaaa 
        tcttagata actagctaa caataagtg cgtctatcc 
        cagttttaa aaataactt ttggtgata cgccaactg 
        atgaactgt ctgttattg cacttgtat atcgtcctc 
        aagcaagaa ttcacttga atggagccc atctccaat 
        tgcaccgag gctgtgact tcggttttc gtcaaactg 
        ctgcaaaac gagaccaaa aatgggcac ttggaccag 
        tttgtcaag ggcttggga cgcatgttc tacatcata 
        gacccttcg gataccacg ttcaaggac caggtgccg 
        aacttttac agaaatgcg tggccctac tttctcctc 
        ttcattctg atcgaaaat ctggtcctt tatctggat 
        aagaagcca acgtttcgc ctaaatgac ggcttgaca 
        agtctatca cacggctta gtgcaagaa tgcggaaga 
        ctaatattc cggggcgct gaaagctac gcttatgtg 
        tatctttac gaaaacttc cggattgtg gaattacgc 
        tgggatcag gcgagtaca tggtacttg gccgccgtc 
        ggagtggat ttctgctat tattgggtg catcgagct 
        tgccatgag gtgcacatc ctctgggcc cagcaccag 
        gtccaccac agctcggag gagttcaac ctcgctgta 
        ggcctccgg caaagcctc ctccaaggc tggtgcggc 
        tttatattt tatttgcct ctggctttc gccattcct 
        ccgacgcat ttcctcacc caccagcaa ttcaatttg 
        ctttatcaa ttctggata cataccaag gctgttaag 
        acgctagga ccgttggaa ttttttttt aatacgccg 
        cagcatcat cgagttcat cacggtgct aatatttgg 
        tgcctcgat aaaaattac ggcggggtt ttgattatt 
        tgggaccgg atttttggg acgtttgct gaagaaagg 
        aaaaatgaa gagattgtc tatggtttg gttttcaat 
        caaccgtct ttcaatatt ttacattta caaactttt 
        tatacagcg tatgttttg caacgattt cgggaaatg 
        aactgttgg cggcataag ttggctgcg attttttat 
        gggccgagt tggcagcct ggaaggccg agattagga 
        gtagatgag gataagttc aaagtagtt ccccggcag 
        aaatacgac gtgaaattg ccgctctgg tgtaatatc 
        tacctcttg gtccacttc tgcatcgtc gtttacggc 
        tttcaagaa ttagcaagt cgccatttg gtcctcaac 
        cccgtcaca gtcctagcg ttcgttatc tacatcatt 
        gcatcgctc acgtcaata ggactgttg ttcgacaat 
        gcgccctac gcctgcatt atggagctc ttccggtgc 
        atggtcctc gttaccgcc ctacaaaag ctggaattt 
        aaggatgtg gataatggg gtgttggtc ggctgtgag 
        gcgcttttt gtcctctcg ggactcttc tggctgctg 
        cagagcgtc aaagttctg caaatcaag gagatttag 
        tgactgatt tttgtgata tttgttaag tttttcaag 
        tattattta tataaatgt gtgaataaa tgagtgaaa 
        attac
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY