Status
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Gene information
General information
TC004237 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451620.1: 73656..111079
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004237
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03710

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004237 RA
      MALRILQFG IVFAIGYYL GPASPFAGA AGVPDVPLS 
      IDLKDPEDV TVIRGQSAT FRCDATSTT PGVQITWLY 
      NESPIKSND TKRILQEDG SLYFPKVGG GKKTKGLVG 
      EYRCRAMVQ NVSMLSNPA KLKIASFGD FDKQPSNLS 
      VPESQPAVL FCKINSVPA AQIKWEQDY KPLPHNTRH 
      IIKKSQWPW FWGYQVGTL SDRPTGLQA KGVPRPKIT 
      WLKNGEPLP SAARIKKHP SGLVISHTF TSDSGIYQC 
      FAVNAAGKV WAAAQLIPT FVHTPSPPE NVQCRPFDE 
      TSICLTWET PHNVSVKAY SIYCYYTAM GREIPGPDF 
      VTNKTQHLA SGLNSSTNY TCYIRLYSK VASDQSQQV 
      ACKTGVKGR RNLQVIPVN ETSVELSWS KVSTDVPCD 
      GTKHLYKVQ WKKKGRSLV NSKQTEQFS FVISGLSES 
      VEYEFRVIP SWQKSDNSP WVSFTPKGD TSRKSSANT 
      TDLLIPLPP ERLEADKIS ATSVNLTWI DNNENTRFY 
      SVCATETEK HVTDCAEND LMKSAWNHL LITDLQANC 
      SYDFKVRAH NYRGNYGPF SQSVTIRTR ADVPSKVLH 
      LNYEIVNET AVCLHWQAP LHSNGKVTS YLILYTPNA 
      NWPLDEWFN KSVPTFDNS KGCWSVPNN DQTFSSLLV 
      GLDSSKQYT VYVRAASEV GLGNPIYPI KITTRNTNA 
      LAPETQEDV QYNQILGII MGVTISLLF IVVCVSLTI 
      LVRKRCLKS RAFARARMA ASNNYYPAV AQYASEGSS 
      VQVRLENPG SAIHEIEHL VSDDGSNHI PPDTPTHLD 
      TKGTDGFPN GHLNGSAKP YTNGHVPNG IVHITENPR 
      YYMLECNGH SAKKSNPQE TYEEDSNSN LKASKFQDL 
      QRIFETSSK SRQCNRGDS KPSSFKDVR PNG
    CDS sequence
    • >TC004237 RA
      atggcgttg cgaattctt caattcggc attgtgttt 
      gcaatagga tactatctc ggccctgca tcgccgttt 
      gcaggagcc gcaggagtc ccggacgtc cctctgtca 
      atcgacctg aaagaccct gaagatgtg accgtgatc 
      agaggacag tcggccact ttccgctgc gacgccacg 
      tccacaaca cccggtgta caaatcact tggttgtac 
      aatgaaagc ccaattaaa agcaacgac accaagcgc 
      attctccaa gaagatggt tctctctat ttccccaaa 
      gttggaggc ggcaaaaaa accaaaggc cttgtgggc 
      gaataccgc tgccgggcc atggtccaa aacgtttca 
      atgctctca aatccggcg aaactaaaa atcgcctct 
      ttcggtgat ttcgacaaa caaccaagt aatctgagc 
      gttcccgaa tctcaacct gcagtactt ttctgcaag 
      ataaactca gtccctgct gcacaaatt aagtgggag 
      caggattac aaacctcta cctcataac acaaggcac 
      attattaaa aaatcgcag tggccttgg ttttgggga 
      tatcaagtt gggactttg tccgaccgt ccgactgga 
      ctacaggcc aagggcgtc cccaggccg aagatcacc 
      tggctgaag aacggcgag cctttgccg agtgcagct 
      aggatcaag aagcatcca agtgggctt gttatcagc 
      cacacgttc acttctgat tcaggtatt tatcagtgt 
      tttgctgtg aacgcagct gggaaagtt tgggcagct 
      gcacagttg ataccaacg tttgttcac actcctagt 
      cctcctgaa aatgtacaa tgccggccg tttgacgag 
      accagtatt tgtctgacg tgggaaacg ccgcataat 
      gtgtcggtt aaggcctac agtatctat tgctactac 
      acagcaatg ggccgtgag atcccaggg cctgatttc 
      gtcacaaac aagacgcaa catttggcg tctggtctt 
      aattctagt acaaattac acgtgttac attcggttg 
      tattcgaaa gtcgcaagc gaccaatca cagcaagtt 
      gcttgcaag acaggtgtg aaaggacgg agaaatttg 
      caagttatt cctgttaat gagacttcg gttgagtta 
      tcatggagt aaagttagt actgacgtt ccttgcgat 
      ggcacaaaa catttgtac aaagtgcag tggaagaaa 
      aagggacgg tctttggtc aattcaaag cagactgaa 
      caattttca ttcgttatt tctggcctt tcagagtcc 
      gtagaatac gaatttcgg gttattccc agttggcag 
      aaaagcgac aattctccg tgggtctct ttcacccca 
      aaaggtgac actagtcgc aagagcagt gcaaacacg 
      acggatttg ttgatccca ttgcctcct gaacgatta 
      gaagctgat aaaatttca gcaacgagc gtaaacctg 
      acgtggatc gataacaat gaaaacacg agattttat 
      tcagtttgt gcgactgaa actgaaaag catgtgaca 
      gattgcgcc gaaaacgac ttgatgaaa agtgcttgg 
      aatcatttg ttaattacc gatttgcaa gccaactgt 
      tcgtatgat tttaaagtc cgggctcat aattaccgc 
      ggtaattac ggccctttt tcacagtct gttacaata 
      cgcacccgt gctgatgtg ccttcaaaa gttttacac 
      ttgaattac gagattgtg aacgaaact gctgtttgc 
      ttgcattgg caagcacca ttgcactcg aacggcaaa 
      gtcacgagt tacttaatt ctttacaca cctaatgca 
      aactggcct ttggacgaa tggttcaat aaatcggtg 
      ccgactttt gataattca aagggctgt tggtcggtc 
      cctaacaac gaccaaact ttctcctca ctacttgtc 
      ggtctagac tcaagcaag caatacaca gtttacgta 
      agagcagcc tcggaagta ggtctaggc aaccccatc 
      tatccaatc aaaatcaca acacgaaac accaacgcg 
      ttggctcca gaaacgcaa gaggacgtc caatacaat 
      caaatttta ggtattata atgggtgtg acaatttcg 
      ttgctcttc atagtggtg tgtgttagt cttacgatt 
      ttggtgcga aaacgttgc ttgaaatcg cgagcattt 
      gcaagagca agaatggca gcgtcgaat aattattat 
      ccggctgtt gcacaatac gcttcagag ggaagttca 
      gtgcaagtt agattggaa aatccaggc tcggcgatt 
      catgaaatc gagcatttg gtcagtgat gatggctcg 
      aatcatatt ccgcccgat acacccacg catttggac 
      accaagggc acagatgga tttcccaac ggccactta 
      aacggatca gccaagcca tacacaaac ggtcacgtt 
      ccgaacggc attgttcac atcactgaa aatccccga 
      tactacatg ctcgaatgc aacggccac tccgcgaag 
      aaatcaaac ccgcaggaa acgtacgaa gaagactcc 
      aattcgaac ctgaaagcg tcgaaattt caggacctc 
      cagcgcatt ttcgagact tcgtcaaaa agccggcag 
      tgcaatcga ggcgattct aaaccttct agctttaaa 
      gacgtcaga ccgaacgga taa
    mRNA sequence
    • >TC004237 RA
      tgcctatac tatcgaaaa catcgtgcg ttagttaag 
      tgttgtgtg gtgtcttgc gtgttatca aagttccca 
      aatgagtac ttatttatg gcgttgcga attcttcaa 
      ttcggcatt gtgtttgca ataggatac tatctcggc 
      cctgcatcg ccgtttgca ggagccgca ggagtcccg 
      gacgtccct ctgtcaatc gacctgaaa gaccctgaa 
      gatgtgacc gtgatcaga ggacagtcg gccactttc 
      cgctgcgac gccacgtcc acaacaccc ggtgtacaa 
      atcacttgg ttgtacaat gaaagccca attaaaagc 
      aacgacacc aagcgcatt ctccaagaa gatggttct 
      ctctatttc cccaaagtt ggaggcggc aaaaaaacc 
      aaaggcctt gtgggcgaa taccgctgc cgggccatg 
      gtccaaaac gtttcaatg ctctcaaat ccggcgaaa 
      ctaaaaatc gcctctttc ggtgatttc gacaaacaa 
      ccaagtaat ctgagcgtt cccgaatct caacctgca 
      gtacttttc tgcaagata aactcagtc cctgctgca 
      caaattaag tgggagcag gattacaaa cctctacct 
      cataacaca aggcacatt attaaaaaa tcgcagtgg 
      ccttggttt tggggatat caagttggg actttgtcc 
      gaccgtccg actggacta caggccaag ggcgtcccc 
      aggccgaag atcacctgg ctgaagaac ggcgagcct 
      ttgccgagt gcagctagg atcaagaag catccaagt 
      gggcttgtt atcagccac acgttcact tctgattca 
      ggtatttat cagtgtttt gctgtgaac gcagctggg 
      aaagtttgg gcagctgca cagttgata ccaacgttt 
      gttcacact cctagtcct cctgaaaat gtacaatgc 
      cggccgttt gacgagacc agtatttgt ctgacgtgg 
      gaaacgccg cataatgtg tcggttaag gcctacagt 
      atctattgc tactacaca gcaatgggc cgtgagatc 
      ccagggcct gatttcgtc acaaacaag acgcaacat 
      ttggcgtct ggtcttaat tctagtaca aattacacg 
      tgttacatt cggttgtat tcgaaagtc gcaagcgac 
      caatcacag caagttgct tgcaagaca ggtgtgaaa 
      ggacggaga aatttgcaa gttattcct gttaatgag 
      acttcggtt gagttatca tggagtaaa gttagtact 
      gacgttcct tgcgatggc acaaaacat ttgtacaaa 
      gtgcagtgg aagaaaaag ggacggtct ttggtcaat 
      tcaaagcag actgaacaa ttttcattc gttatttct 
      ggcctttca gagtccgta gaatacgaa tttcgggtt 
      attcccagt tggcagaaa agcgacaat tctccgtgg 
      gtctctttc accccaaaa ggtgacact agtcgcaag 
      agcagtgca aacacgacg gatttgttg atcccattg 
      cctcctgaa cgattagaa gctgataaa atttcagca 
      acgagcgta aacctgacg tggatcgat aacaatgaa 
      aacacgaga ttttattca gtttgtgcg actgaaact 
      gaaaagcat gtgacagat tgcgccgaa aacgacttg 
      atgaaaagt gcttggaat catttgtta attaccgat 
      ttgcaagcc aactgttcg tatgatttt aaagtccgg 
      gctcataat taccgcggt aattacggc cctttttca 
      cagtctgtt acaatacgc acccgtgct gatgtgcct 
      tcaaaagtt ttacacttg aattacgag attgtgaac 
      gaaactgct gtttgcttg cattggcaa gcaccattg 
      cactcgaac ggcaaagtc acgagttac ttaattctt 
      tacacacct aatgcaaac tggcctttg gacgaatgg 
      ttcaataaa tcggtgccg acttttgat aattcaaag 
      ggctgttgg tcggtccct aacaacgac caaactttc 
      tcctcacta cttgtcggt ctagactca agcaagcaa 
      tacacagtt tacgtaaga gcagcctcg gaagtaggt 
      ctaggcaac cccatctat ccaatcaaa atcacaaca 
      cgaaacacc aacgcgttg gctccagaa acgcaagag 
      gacgtccaa tacaatcaa attttaggt attataatg 
      ggtgtgaca atttcgttg ctcttcata gtggtgtgt 
      gttagtctt acgattttg gtgcgaaaa cgttgcttg 
      aaatcgcga gcatttgca agagcaaga atggcagcg 
      tcgaataat tattatccg gctgttgca caatacgct 
      tcagaggga agttcagtg caagttaga ttggaaaat 
      ccaggctcg gcgattcat gaaatcgag catttggtc 
      agtgatgat ggctcgaat catattccg cccgataca 
      cccacgcat ttggacacc aagggcaca gatggattt 
      cccaacggc cacttaaac ggatcagcc aagccatac 
      acaaacggt cacgttccg aacggcatt gttcacatc 
      actgaaaat ccccgatac tacatgctc gaatgcaac 
      ggccactcc gcgaagaaa tcaaacccg caggaaacg 
      tacgaagaa gactccaat tcgaacctg aaagcgtcg 
      aaatttcag gacctccag cgcattttc gagacttcg 
      tcaaaaagc cggcagtgc aatcgaggc gattctaaa 
      ccttctagc tttaaagac gtcagaccg aacggataa 
      tgagcgaat atttcatag gcaaattca cgaccaatc 
      atccgctta atttatttt ccttgcttt caccggatt 
      gctcaatgt cttgtgttc agctgcggc cacgcgttg 
      acttcgtgc gttttaccg aagggttgc agctgaagt 
      tcaggtttt ttttatttt ttttttcgt agcataata 
      caactggta cctgaaaac aattacata tatgatagc 
      gtagacgta agtaaaatg ttactgtac agtatatat 
      tgtgggtga gtgcgggag cgcttgttt gtgtctgat 
      tgttacatg ttatatatg gctacaatg ttaactgtt 
      cttcattaa cgttaccaa caaagcata tttattatt 
      tatataata tccaaagaa tattccatt gtttaaacg 
      atgtacaaa attgtttag aaaatatta tcctctcat 
      tcagtcaca attcaatgt ggtgtgtgc ctttacatc 
      gtacaaaac aacgagcgt ttagttacg tacagtagg 
      cgtaagata ttcagttac cgtgtacag tcacgcccg 
      aaaagaacg acaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY