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Gene information
General information
TC002031 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid402: 8856..11087
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002031
      iBeetle screen iB_03288
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      iBeetle screen iB_03708
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 4 (16 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002031 RA
        MDCAVSKSS FSFVPEDSF HPSLEFTLD IVAKTPDKF 
        YMNRDHVAF NFKKANFEC LYTTLQVVD WSELEKFSD 
        VNEACRVFY ETLYNCFEL SVSKLFCRK NYRTFPTWF 
        NTDIINCLR RKSNILKKY RRTKNMHFY EEFKKLRSL 
        SKSLITTAY SQYIGHMEN TISKDPKKF WSFVQAKKR 
        NSRIPGVLY HNGNAISNP NDIVNSFAK FFKSVFSDM 
        TLTSDSPSM MTNLNSFIT VNEINEHEI IRVLKRSKD 
        TLTAGCDGI PSFLLRDCA HIFSKPLVY IFNLILQTS 
        TFPNIWKIA HVYPVFKTG DITQIQNYR PISILCNFS 
        KVFESIIYD RIYSSVKRF ISPSQHGFV EKRSTVTNL 
        ACFTQFISD ALDRKGQVD VIYMDFKKA FDQIDHHLL 
        LLKLDQYGF SGSLLSLIK SYLANREQR VKYRNYISD 
        SYVATSGVP QGSNLGPLL FLLFINDIC GSLSTCAKL 
        LFADDLKIY TEIKSIEDC LTLQNNINA VVKWCNENR 
        LYLNPSKCN VMSYTKKRE FLEFVYDIS SVTLHRTFI 
        IKDLGVIFD TELAFSEHI RDVAARAIK SYGFIYRNC 
        RDFKNLSVM KTLFFSLVR SKLEYGALI WHPIYKIHI 
        DQLENIQRR FLKFLVFII DGNYPIRGY DQNLLLNRF 
        GLQSLHFRR ICIIIKFLY NLINNNIDC SWLLNMLNF 
        AVPRLNSRQ AMTFYPIAR RTNVADKSP IVLMTTLFN 
        NVCKDGDIN YDSWRKIVS SISLV
      CDS sequence
      • >TC002031 RA
        atggactgt gccgttagc aagtcctct ttctcgttt 
        gttcctgaa gattctttc catccgagt ttggagttt 
        accctcgat attgtagca aaaactcct gataagttc 
        tatatgaat agggaccac gtcgcattt aattttaaa 
        aaagcaaat tttgaatgt ctatacact actttacag 
        gtagttgat tggtccgaa ctggagaag tttagcgat 
        gtgaatgag gcatgtcga gttttttat gaaaccctc 
        tacaattgt tttgagcta tctgtttca aaactattt 
        tgtcgtaaa aactaccgc acatttcct acttggttc 
        aacactgac attatcaat tgtctacgt cgtaaatct 
        aacatttta aagaaatat cgtcgaact aaaaatatg 
        catttctat gaggagttt aagaaactt cgatctctt 
        tcaaaatcc ctaataact actgcctat tctcaatac 
        atcggccac atggaaaac actatctcc aaagatcca 
        aagaaattc tggtctttt gttcaagct aaaaagcgt 
        aactctaga attccgggt gtgttgtat cataatggt 
        aatgcaata tcaaatcct aatgatata gtaaatagt 
        ttcgctaaa ttcttcaaa agtgttttt tctgacatg 
        acattgact tcagatagt ccctcgatg atgacgaat 
        ctaaatagt tttattact gtaaacgag attaatgag 
        catgaaata atccgggta ttaaagcga tctaaagac 
        acacttact gctggttgt gatggtatc cccagcttc 
        ttattaagg gattgtgca catattttt tctaaacca 
        ctcgtatac atttttaat ttaatattg caaacttcg 
        acgtttcct aacatatgg aaaatagca cacgtttat 
        cctgtcttc aaaacgggt gatataaca caaattcaa 
        aactacagg ccaatttca atcttgtgt aatttctcg 
        aaagttttt gagtcaatt atttacgac cgcatctac 
        tcttctgta aaaagattt atatcacct tcgcagcat 
        ggatttgtt gaaaaaaga tcaactgtt acaaatcta 
        gcctgtttt actcagttc atatcggac gctttggac 
        agaaaaggt caggttgat gtcatttat atggacttc 
        aaaaaagca tttgatcaa attgatcat catctgtta 
        ttgttaaaa cttgatcag tatggtttc tccggttct 
        ttgctatcc ctaataaaa tcttatctt gctaatcga 
        gagcaaagg gttaaatat cgtaactac atttcggat 
        agttacgtt gcaacttcg ggtgtgccg caaggttcc 
        aatcttgga cccttatta tttttgctt ttcattaat 
        gatatatgt ggttcttta tcaacatgc gctaaactt 
        ctctttgcc gatgatctt aaaatttac acagaaata 
        aaatctata gaggactgc ctcacttta cagaacaat 
        attaacgca gtagtgaaa tggtgtaat gaaaatcgc 
        ctttatcta aacccatcc aaatgtaac gttatgtct 
        tacactaaa aagcgcgaa ttccttgaa tttgtatat 
        gatatttcg tccgttaca ctgcatcgt acctttatt 
        attaaagat ttgggagtt attttcgat actgaactc 
        gcattcagt gagcatatt agggatgtt gcggctaga 
        gcgattaag tcatatgga ttcatctat agaaattgt 
        cgagatttt aagaactta tcagttatg aagacccta 
        ttcttctcg ttagttaga agcaaactg gaatacggc 
        gcacttatt tggcacccg atttataaa atccatata 
        gatcaactc gaaaacata cagcgacga ttcctaaaa 
        ttcctagtt tttataata gatggaaat tatcctata 
        aggggctat gaccagaac ttgcttttg aatagattc 
        ggtctccaa tctttacat tttcgccgt atttgtatt 
        ataattaaa tttctgtat aatttaatt aataataac 
        atcgactgt tcttggcta ttaaatatg ttaaatttt 
        gctgttcca cgtttaaac tccagacaa gccatgaca 
        ttttatcct atagcgaga aggaccaat gtagctgat 
        aagtctcct atcgtatta atgactacc ctatttaat 
        aatgtatgt aaagacggt gatatcaac tatgattcg 
        tggcggaag attgtttcg tctatatca ttagtatag
      mRNA sequence
      • >TC002031 RA
        atggactgt gccgttagc aagtcctct ttctcgttt 
        gttcctgaa gattctttc catccgagt ttggagttt 
        accctcgat attgtagca aaaactcct gataagttc 
        tatatgaat agggaccac gtcgcattt aattttaaa 
        aaagcaaat tttgaatgt ctatacact actttacag 
        gtagttgat tggtccgaa ctggagaag tttagcgat 
        gtgaatgag gcatgtcga gttttttat gaaaccctc 
        tacaattgt tttgagcta tctgtttca aaactattt 
        tgtcgtaaa aactaccgc acatttcct acttggttc 
        aacactgac attatcaat tgtctacgt cgtaaatct 
        aacatttta aagaaatat cgtcgaact aaaaatatg 
        catttctat gaggagttt aagaaactt cgatctctt 
        tcaaaatcc ctaataact actgcctat tctcaatac 
        atcggccac atggaaaac actatctcc aaagatcca 
        aagaaattc tggtctttt gttcaagct aaaaagcgt 
        aactctaga attccgggt gtgttgtat cataatggt 
        aatgcaata tcaaatcct aatgatata gtaaatagt 
        ttcgctaaa ttcttcaaa agtgttttt tctgacatg 
        acattgact tcagatagt ccctcgatg atgacgaat 
        ctaaatagt tttattact gtaaacgag attaatgag 
        catgaaata atccgggta ttaaagcga tctaaagac 
        acacttact gctggttgt gatggtatc cccagcttc 
        ttattaagg gattgtgca catattttt tctaaacca 
        ctcgtatac atttttaat ttaatattg caaacttcg 
        acgtttcct aacatatgg aaaatagca cacgtttat 
        cctgtcttc aaaacgggt gatataaca caaattcaa 
        aactacagg ccaatttca atcttgtgt aatttctcg 
        aaagttttt gagtcaatt atttacgac cgcatctac 
        tcttctgta aaaagattt atatcacct tcgcagcat 
        ggatttgtt gaaaaaaga tcaactgtt acaaatcta 
        gcctgtttt actcagttc atatcggac gctttggac 
        agaaaaggt caggttgat gtcatttat atggacttc 
        aaaaaagca tttgatcaa attgatcat catctgtta 
        ttgttaaaa cttgatcag tatggtttc tccggttct 
        ttgctatcc ctaataaaa tcttatctt gctaatcga 
        gagcaaagg gttaaatat cgtaactac atttcggat 
        agttacgtt gcaacttcg ggtgtgccg caaggttcc 
        aatcttgga cccttatta tttttgctt ttcattaat 
        gatatatgt ggttcttta tcaacatgc gctaaactt 
        ctctttgcc gatgatctt aaaatttac acagaaata 
        aaatctata gaggactgc ctcacttta cagaacaat 
        attaacgca gtagtgaaa tggtgtaat gaaaatcgc 
        ctttatcta aacccatcc aaatgtaac gttatgtct 
        tacactaaa aagcgcgaa ttccttgaa tttgtatat 
        gatatttcg tccgttaca ctgcatcgt acctttatt 
        attaaagat ttgggagtt attttcgat actgaactc 
        gcattcagt gagcatatt agggatgtt gcggctaga 
        gcgattaag tcatatgga ttcatctat agaaattgt 
        cgagatttt aagaactta tcagttatg aagacccta 
        ttcttctcg ttagttaga agcaaactg gaatacggc 
        gcacttatt tggcacccg atttataaa atccatata 
        gatcaactc gaaaacata cagcgacga ttcctaaaa 
        ttcctagtt tttataata gatggaaat tatcctata 
        aggggctat gaccagaac ttgcttttg aatagattc 
        ggtctccaa tctttacat tttcgccgt atttgtatt 
        ataattaaa tttctgtat aatttaatt aataataac 
        atcgactgt tcttggcta ttaaatatg ttaaatttt 
        gctgttcca cgtttaaac tccagacaa gccatgaca 
        ttttatcct atagcgaga aggaccaat gtagctgat 
        aagtctcct atcgtatta atgactacc ctatttaat 
        aatgtatgt aaagacggt gatatcaac tatgattcg 
        tggcggaag attgtttcg tctatatca ttagtatag