Status
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Gene information
General information
TC031650
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016047,TC016048,TC016046
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid550: 7279..20364
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031650
      iBeetle screen iB_03705
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (41 dpi)
      Days post injection


      iBeetle screen iB_03891
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
      Days post injection


      iBeetle screen iB_05950
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031650 RA
        MRVTSYIRP TQRRTPIYL IVRYPRLKV VDMENNRNT 
        GNAGAPPQE MPTWFVRWL NSQQPRNAP NFAAPSTST 
        AVQRPQAVL QVRPSTSAA ADADGWQVT PLPSDGTTS 
        PEPDPEIPV APEPAPLAS PLVQEPGSS TTSATSGAV 
        MASPPMPIT TDNVAAISG VLRSLLDRP LPAPERPSF 
        EGLKRQNSY EPLKNMDKK WRGYEDFRR DFLRTYWSA 
        KRQRDIRFQ IATGRYDET RGTMLSHFA YYVDMANML 
        TTPLSEEVL LDELLRHFP ERIQSLWVL EKICTTTDA 
        AEFLAAQKI QGQNPGEKT NIAPRERPR ATDHQLRNE 
        PKRPRPNID RHEVTRPAA PANRGNGFQ KRSSDNQQW 
        RNNQPSTSN NRWHNNNES NSNNHWRNN QPSTSNNRW 
        HNNNGNNSN NHWRRNNPN DNRNNTSSR AQERGLRYS 
        GQQHGPQSD SRNQQLRTG EKRKRSTSS QSTEGENDA 
        RPRASQVKL NVFSIIKSA VAGNNNKQR VVSEIKIQI 
        SQQQSVTAL LDIGSEVSC ISEEVWSKL IETGNKPPT 
        LPVTSIHLR GAIGQRSCQ PTILGADWL NEQGAVIDF 
        ENNKIPLRN GEKHHSFPL RKTTEIPPE PEDYVEDLV 
        GHIEVNHST PITTFERLT RWTLILGQY DYEIELVKG 
        KDNVVADIL SRYPSDGEA SYEYPREQP IVAMFEVNN 
        TPEILQLMS DLKQHQSDD PVLSQIIAN KQSGAAAPD 
        ARTQRVWSQ YNFTNNVLV HRSQNSNDV CLAIPESLV 
        IKLVDYHHQ LLGHFGATK VYNSMKREY YWPNMYRTI 
        KKRLRSCDL CQKTKSSNR PHQGPLTPI LYDHIGDLV 
        CVDFYGPLP TGRLGASYV FVVIDVFSK FLKLYPLRK 
        ATAKIAAKR LIEDFSGYI KPKCVLSDH GTQFISNTW 
        QNSLRAADI QPTLSSIRH PESNPSERV MRELGRIFR 
        AYCRENHAS WVNHLSNIE DCLNYVPHI STGFSPYEI 
        LYGRTPPNP LDAVTSGLL PVRPPLTRE EIHEKALEN 
        LRHHANLRQ KNQKGEVTI LKIKDWVLL KNKVTSDTR 
        THQFAKFMP LYSGPYKII AKPHPNTYQ IADPVVDME 
        NNRNTGNAG APPQEMPTW FVRWLNSQQ PRNVPNFAA 
        PSTSTAVQR PQAILQVRP STSAAADVD GWQVTPLPS 
        DGTTSPEPD PEIPVAPEP APLASPLVQ EPGSSTTSA 
        TSGAVMASP PMPITTDNV AAISGVLRS LLDRPLPAP 
        ERPSFEGLK RQNPVKFLR AIEEYGRFF GLDSQRLLG 
        VAMDCLKGN AKHWTGIFQ KKWRGYEDF RRDFLRTYW 
        SAKRQRDIR FQIATGRYD ETRGTMLSH FAYYVDMAN 
        MLTTPLSEE VLLDELLRH FPERIQSLW VLEKICTTT 
        DAAEFLAAQ EIPGQNPGE KTNIAPRER PRATDHQRR 
        NEPKRPRPN IDRHEVTRP AAPANRGNG FPKRSSDEQ 
        QWRNNQPST SNNRWHNNN GNNSNNHWR KNNPNDNRN 
        NTSSRAQGR GLKYSGQQH GPQSDSRNQ QLRTGEKRK 
        RSTSSQSTE GENDARPRA SQVKLNVFS IIKSAAAGN 
        NNIQRVVPE IKIQISQQQ SVTALLDTG SEVSCISEE 
        VWSKLIETG NKPPTLPVT SIHLRGAIG QRSCQVVIQ 
        CYLEIKIDE HLYPVVALV VKNLIKPTI LGADWLNEQ 
        RAVIDFDNN EILLRNGEK HHSFPFRKT TEIPPEPED 
        YVEDLVGHI EVNHSAPIT TCNKKQHLE SPSALQAKV 
        DPLKIPEAE KRKLIHLLQ EYRCIFSLR PGLTHKYTH 
        EIKLHDKTP FLKRPYPVP FALRPAVDA TIQEMLDLG 
        VIKREASPY ASPMTVVKK KDGTVRICL DARMINSKM 
        IADCESPPA ADELLRRFH GIRYMSTID LRSSYWQIP 
        LSPESRQYT AFLYNGRSY TYQVLPFGL KTAVGSFSR 
        AMDVVLGTE VREFVVNYI DDLLVASET LNEHLEHLR 
        QVFEKLKQA RMTINLEKS NFIQKEPTR KKISAIRNF 
        PVPQKTKHV RAFLGLCNF YRKFCARYS AATQDVNKL 
        LRKGEKWRW GRNEQEAFD RVKDLFLEA VLLRYPDLN 
        KIFYVQTDS SGYGLGAEL YQIQEDGSR GVIAFASRS 
        LKGPELNYT TTEKELLGV IFALHKFRI YIQVTKIII 
        RTDHQALKF LSRCRLLSE RLTRWTLIL GQYDYEIEL 
        VKGKDNVIA DILSRYPSD GEASYEYPR EQPIVAMFE 
        VNNTPEILQ LMSDLKQHQ SDDPVLSQI IANKQSGAA 
        APDARTQRV WSQYNFTNN VLVHRSQNS NDVCLAIPE 
        SLVIKLVDY HHQLLGHFG ATKVYNSMK REYYWPNMY 
        RTIKKRLRS CDLCQKTKS SNRPHQGPL TPILYDHIG 
        DLVCVDFYG PLPTGRLGA SYVFVVIDV FSKFLKLYP 
        LRKATAKIA AKRLIEDFS GYIKPKCVL SDHGTQFIS 
        NTWQNSLRA ADIQPTLSS IRHPESNPS ERVMRELGR 
        IFRAYCKEN HTSWVNHLS NIEDCLNYV PHISTGFSP 
        YEILYGRTP PNPLDAVTS GLLPVRPPL TREEIHEKA 
        REYLRHHAN LRQKNQKGE VTVLEIEDW VLLKNKVTS 
        DTRTHQFAK FMPLYSGPY KIIAKPHPN TYQIADPVT 
        NEIKGVYNM TNLKFYHRR DD
      CDS sequence
      • >TC031650 RA
        atgagagtg acttcgtac atcagacca acacaacgt 
        aggactcca atttatttg atcgttcga tacccgaga 
        ttgaaggtt gtcgacatg gaaaacaac aggaacacc 
        ggaaacgcc ggagcccca ccgcaagaa atgccgacg 
        tggttcgtt cgttggtta aactcccaa caaccaagg 
        aatgcgcca aacttcgcg gcgccatcg acgtcgaca 
        gcggttcaa agaccgcaa gccgtcctt caggtacgg 
        ccaagtacc agcgccgcc gctgatgcc gatggctgg 
        caagtcact ccactgcct agcgacggg acgacttcg 
        ccagaaccc gacccggaa attccggta gctccggaa 
        ccagcccct cttgcgagc cctcttgtg caagagccg 
        ggaagttcg accacatca gcgacttcg ggagcagtg 
        atggcctca ccacctatg ccaatcaca acagacaat 
        gtggccgca atttccgga gtactccgg agcttgctg 
        gatcgccca cttccagct cctgaacga ccatctttc 
        gaaggcctg aaaaggcaa aattcttac gagccattg 
        aagaatatg gacaagaag tggcgtggt tacgaggac 
        tttcgacga gactttctc cggacctac tggtcggcc 
        aagcgtcaa cgggacatc agatttcaa atcgctaca 
        ggccgctac gacgaaacc aggggcacg atgctgtcg 
        cattttgct tattacgtc gatatggcg aacatgtta 
        acaacacct ttatcggag gaagtgttg ctggatgaa 
        ttgcttcgt cacttcccc gaaagaata cagtcgttg 
        tgggtatta gagaaaatc tgtaccacc actgatgcc 
        gccgaattc ctggcagct caaaaaatt caggggcaa 
        aatccggga gagaaaacc aacatcgct cccagggaa 
        cgaccccgc gcaacagac caccagctg cgtaacgag 
        ccaaagcgc ccgcggcca aacattgac cgccacgaa 
        gtaactcgt cctgcggcc cctgcaaat cgtggaaat 
        ggtttccaa aaacgctca agtgacaac caacaatgg 
        cgcaacaac caacccagc accagcaac aaccgttgg 
        cacaacaac aacgagagc aacagcaac aatcactgg 
        cgcaacaac caacccagc accagcaac aaccgttgg 
        cacaataac aacggaaac aacagcaac aatcactgg 
        cgcagaaac aaccccaac gacaaccgc aacaacaca 
        agttcgaga gcgcaagaa cggggccta aggtactcc 
        ggccagcag cacggccca caaagcgac agcaggaac 
        caacaattg aggacggga gagaaacgt aaacgttca 
        acttcgtca caatcaaca gaaggggaa aacgatgct 
        cgaccgcga gcatcccag gttaagtta aatgttttt 
        tctataatt aaatccgct gtcgcaggt aacaacaac 
        aaacagagg gtcgtgtcg gagattaag atccaaatc 
        tcccaacaa caatcagtc accgcgctt ttggacatc 
        ggaagtgaa gtcagctgt atttccgaa gaagtttgg 
        tccaagctg atcgagacc ggaaacaaa ccgcccacg 
        ttaccggtt acctcaatc catcttcgt ggagccatc 
        ggccagcga agttgccag ccaaccatt ttgggagca 
        gactggcta aatgagcaa ggcgcggtc atcgatttc 
        gaaaacaac aaaatccct ttgagaaac ggggagaag 
        caccacagt tttccgtta agaaaaacc acggagata 
        ccaccggaa ccggaggat tatgtggaa gatcttgtt 
        ggtcacatc gaagtcaat cattcgacg ccgatcacg 
        acttttgaa cgcctaact cgatggact ttgatacta 
        ggacagtac gactacgaa atcgagttg gtaaaagga 
        aaggataat gtagttgcc gacatcctg tcacgatac 
        ccgtccgac ggtgaagcc agttatgaa tatccacgt 
        gaacaacca atagttgca atgtttgaa gtcaacaac 
        acacctgaa attctccaa ctgatgtcc gatctcaaa 
        caacaccaa tcagatgat cctgttttg agtcaaata 
        attgccaac aaacaatcc ggtgcagct gctccagat 
        gcgagaact caacgggtt tggtcccaa tataatttc 
        acaaataat gtgttagtt cacagatct caaaattca 
        aacgacgtt tgcctggcc atacctgag agtttggta 
        attaagctc gtggattac catcaccaa ctcctgggc 
        catttcggg gcaaccaaa gtgtacaat tccatgaag 
        cgcgagtac tactggccg aacatgtat cgtacaatt 
        aagaaacgt ctacgaagt tgtgacctt tgtcagaag 
        acaaaaagt tccaaccgt ccacatcag ggtccatta 
        acgccaatc ctttacgac cacattgga gacctagtt 
        tgcgtcgat ttctatggg cctttacca acaggtcga 
        ttgggggcg tcctatgta tttgtggtc attgacgtc 
        ttcagcaag tttctcaaa ctttaccca ctgagaaaa 
        gccaccgcc aaaatcgct gccaaacgt ctcatagag 
        gatttctct gggtacatc aaacccaaa tgtgtgtta 
        agcgaccac gggacacaa tttatttcg aacacttgg 
        cagaatagt ttgagagct gccgacatt caaccaacg 
        ctctcgtcg attcgtcac ccagaatcg aaccctagc 
        gagcgcgta atgcgggag ctggggaga attttccga 
        gcgtattgt agagaaaac cacgccagt tgggtcaac 
        catttgagc aacatcgaa gattgtttg aattacgtg 
        ccacacatc agtaccgga ttttctcca tatgaaatc 
        ctgtacggc agaacacca ccaaaccca ctagatgca 
        gtcacatcg ggtttactg cctgtacgt ccgccactc 
        acgagagaa gaaattcat gaaaaggct ctcgaaaac 
        ttgagacat cacgcgaac ttacggcag aaaaaccaa 
        aagggtgaa gttaccata ctgaaaatt aaagattgg 
        gtcctactg aagaacaaa gtaacttcc gataccaga 
        acccaccaa ttcgccaag ttcatgcct ttgtacagt 
        ggtccgtac aaaataatc gccaagcct catccaaac 
        acataccaa atcgccgac ccagttgtc gacatggaa 
        aacaacagg aacaccgga aacgccgga gccccaccg 
        caagaaatg ccgacgtgg ttcgttcgt tggttaaac 
        tcccaacaa ccaaggaat gtgccaaac ttcgcggcg 
        ccatcgacg tcgacagcg gttcaaaga ccgcaagcc 
        atccttcag gtacgtcca agtaccagc gccgccgct 
        gatgtcgat ggttggcaa gtcactcca ctacctagc 
        gacgggacg acttcgcca gaacccgac ccggaaatt 
        ccggtagct ccggaacca gcccctctt gcgagccct 
        cttgtgcaa gagccggga agttcgacc acatcagcg 
        acttcggga gcagtgatg gcctcacca cctatgcca 
        atcacaaca gacaatgtg gccgcaatt tccggagta 
        ctccggagc ttgctggat cgcccactt ccagctcct 
        gaacgacca tctttcgaa ggcctgaaa aggcaaaat 
        ccggtgaaa ttcttacga gccattgaa gaatatgga 
        cgttttttc gggctcgac tcgcagcgt cttttggga 
        gtcgccatg gattgcttg aaaggcaat gccaaacat 
        tggacggga atattccag aagaagtgg cgtggttac 
        gaggacttt cgacgggac tttctccgg acctactgg 
        tcggccaag cgtcaacgg gacatcaga tttcaaatc 
        gctacaggc cgctatgac gaaaccagg ggcacgatg 
        ctgtcgcat tttgcttat tacgtcgac atggcgaac 
        atgttaaca acaccttta tcggaggaa gtgttgctg 
        gatgaatta ctgcgtcac ttccccgaa agaatacag 
        tcgttgtgg gtattagag aaaatctgt accaccacg 
        gatgccgcc gaattcctg gcagctcaa gaaattccg 
        ggacaaaat ccgggagag aaaaccaac atcgctccc 
        agggaacga ccccgcgca acagaccac cagcggcgt 
        aacgagcca aagcgcccg cggccaaac attgaccgc 
        cacgaagta actcgtcct gcggctcct gcaaatcgt 
        ggaaatggt ttcccaaaa cgctcaagt gacgaacaa 
        caatggcgc aacaaccaa cccagcacc agcaacaac 
        cgttggcac aataacaac ggaaacaac agcaacaat 
        cactggcgc aaaaacaac cccaacgac aaccgcaac 
        aacacaagt tcgagagcg cagggacgg ggcctaaag 
        tactccggc cagcagcac ggcccacaa agcgacagc 
        aggaaccaa caattgagg acgggagag aaacgtaaa 
        cgttcaact tcgtcacag tcaacagaa ggggaaaac 
        gatgctcga ccgcgagca tcccaggtt aagttaaat 
        gttttttct ataattaaa tccgctgcc gcaggtaac 
        aacaacata caaagggtc gtgccggag attaagatc 
        caaatctct caacaacaa tcagtcacc gcacttttg 
        gacaccgga agtgaagtc agctgtatt tccgaagaa 
        gtttggtcc aagttgatc gagaccgga aacaaaccg 
        cccacatta ccggttacc tcaatccat cttcgtgga 
        gccatcggc cagcgaagt tgccaggtt gttattcag 
        tgttacctg gaaatcaaa atcgacgaa catctctac 
        cccgtcgtg gcgcttgtc gttaaaaat ttaatcaag 
        ccaaccatt ttgggagca gactggcta aatgaacaa 
        cgcgcggtc atcgatttc gataacaac gaaatcctt 
        ttgagaaac ggggagaag caccacagt tttccgttt 
        agaaaaacc acggagata ccaccggaa ccggaggat 
        tatgtggaa gatcttgtt ggtcacatc gaagtcaat 
        cattcggcg ccgatcacg acttgtaac aaaaagcaa 
        cacttagag tcacccagt gccctacaa gccaaggtg 
        gacccatta aaaattcct gaggccgag aaacggaaa 
        ttaatacac ctgctgcaa gagtacaga tgtatcttt 
        agtttgcgt ccagggcta acacacaaa tacactcac 
        gaaataaaa ctacacgac aagactccc ttcttgaaa 
        cgaccgtac ccagtacct tttgctcta cgtcctgcc 
        gtcgacgcc acaattcag gaaatgtta gacttgggg 
        gtgataaaa cgtgaggcg tcaccttac gcaagtccc 
        atgacggta gtgaaaaag aaagatgga acggtgaga 
        atttgcctc gacgcccga atgatcaat tccaaaatg 
        atcgccgat tgtgagtca ccacccgct gctgacgaa 
        ttattacgt cgatttcac ggaattcgc tacatgtcc 
        accattgat ttgagatcg tcgtactgg caaatccca 
        ctttcgcca gaaagcaga cagtacacc gcctttctg 
        tacaacggt cgcagttac acataccaa gtcctacct 
        ttcgggtta aaaactgct gtgggttca ttcagtcgt 
        gcaatggac gtagtgtta ggaacagaa gttcgcgaa 
        ttcgtcgtg aattatatc gacgatttg ttagtggct 
        tccgaaacg ctaaacgaa catttagaa catctccga 
        caagttttc gaaaaattg aagcaagct agaatgacg 
        ataaatctc gaaaaatcc aatttcata caaaaggaa 
        ccgacccgg aaaaaaatc agcgccatc cgaaatttt 
        ccggttccc cagaaaact aaacacgtt cgagccttc 
        ctgggactt tgtaatttt tatcgaaaa ttttgtgct 
        cgatacagt gcagccaca caagacgta aacaaacta 
        cttcgaaag ggtgaaaaa tggagatgg ggacgaaat 
        gaacaagaa gctttcgat cgtgtgaaa gatctgttc 
        ctcgaagct gtgttgtta cgttacccc gacctgaat 
        aaaatattc tacgtccaa actgacagt tctggttac 
        ggattgggg gcggaattg tatcaaatt caggaagac 
        ggttcgcga ggtgtgatc gcttttgca agtcgctct 
        ttgaaagga cccgaactc aactacacg actacagaa 
        aaggaatta ctcggcgtc atttttgcc ttacacaaa 
        tttcgaatc tacatccaa gtcacaaaa atcatcatc 
        cgaaccgac caccaagct ttgaaattt ctgagccgt 
        tgtcgtttg ctcagtgaa cgcctaact cgatggact 
        ttgatacta ggacagtac gactacgaa atcgagttg 
        gtaaaagga aaggataat gtaattgcc gacatcctg 
        tcacgatac ccgtccgac ggtgaagcc agttatgaa 
        tatccacgt gaacaacca atagttgca atgtttgaa 
        gtcaacaac acacctgaa attctccaa ctgatgtcc 
        gatctcaaa caacaccaa tcagatgat cctgttttg 
        agtcaaata attgccaac aaacaatcc ggtgcagct 
        gctccagat gcgagaact caacgggtt tggtcccaa 
        tataatttc acaaataat gtgttagtt cacagatct 
        caaaattca aacgacgtt tgcctggcc atacctgag 
        agtttggta attaagctc gtggattac catcaccaa 
        ctcctgggc catttcggg gcaaccaaa gtgtacaat 
        tccatgaag cgcgagtac tactggccg aacatgtat 
        cgtacaatt aagaaacgt ctacgaagt tgtgacctt 
        tgtcagaag acaaaaagt tccaaccgt ccacatcag 
        ggtccatta acgccaatc ctttacgac cacattgga 
        gacctagtt tgcgtcgat ttctatggg cctttacca 
        acaggacga ttgggggcg tcctatgta tttgtggtc 
        attgacgtc ttcagcaag tttctcaaa ctttaccca 
        ctgagaaaa gccaccgcc aaaatcgct gccaaacgt 
        ctcatagag gatttctct gggtacatc aaacccaaa 
        tgtgtgtta agcgaccac gggacacaa tttatttcg 
        aacacttgg cagaatagt ttgagagct gccgacatt 
        caaccaacg ctctcgtcg attcgtcac ccagaatcg 
        aaccctagc gagcgcgta atgcgggag ctggggaga 
        attttccga gcgtattgt aaagaaaac cacaccagt 
        tgggtcaac catttgagc aacatcgaa gattgtttg 
        aactacgtg ccacacatc agtaccgga ttttctcca 
        tatgaaatc ctgtacggc agaacacca ccaaaccca 
        ctagatgca gtcacatcg ggtttactg cctgtacgt 
        ccgccactc acgagagaa gaaattcat gaaaaggct 
        cgcgaatac ttgagacat catgcgaac ttacggcag 
        aaaaaccaa aagggtgaa gttaccgta ctggaaatt 
        gaagattgg gtcctactg aagaacaaa gtgacttcc 
        gataccaga acccaccaa ttcgccaag ttcatgcct 
        ttgtacagt ggtccgtac aaaataatc gccaagcct 
        catccaaac acataccaa atcgccgac ccagtgacg 
        aacgaaatt aaaggtgta tacaacatg acaaatttg 
        aaattctac caccgacga gacgactaa
      mRNA sequence
      • >TC031650 RA
        atcaaactt tcataacca ttgattatt gtaatccat 
        acaacggaa tattctttc ttcttgtaa taaataact 
        ttggaggaa acttgtggt ttaaatccc ttctcccat 
        gcgttttga cactacttt gaatccgga cttgcgtta 
        cggataaat cctgaaccc tttctgata agaattaag 
        atcaaattg cgatatcac tatgagagt gacttcgta 
        catcagacc aacacaacg taggactcc aatttattt 
        gatcgttcg atacccgag attgaaggt tgtcgacat 
        ggaaaacaa caggaacac cggaaacgc cggagcccc 
        accgcaaga aatgccgac gtggttcgt tcgttggtt 
        aaactccca acaaccaag gaatgcgcc aaacttcgc 
        ggcgccatc gacgtcgac agcggttca aagaccgca 
        agccgtcct tcaggtacg gccaagtac cagcgccgc 
        cgctgatgc cgatggctg gcaagtcac tccactgcc 
        tagcgacgg gacgacttc gccagaacc cgacccgga 
        aattccggt agctccgga accagcccc tcttgcgag 
        ccctcttgt gcaagagcc gggaagttc gaccacatc 
        agcgacttc gggagcagt gatggcctc accacctat 
        gccaatcac aacagacaa tgtggccgc aatttccgg 
        agtactccg gagcttgct ggatcgccc acttccagc 
        tcctgaacg accatcttt cgaaggcct gaaaaggca 
        aaattctta cgagccatt gaagaatat ggacaagaa 
        gtggcgtgg ttacgagga ctttcgacg agactttct 
        ccggaccta ctggtcggc caagcgtca acgggacat 
        cagatttca aatcgctac aggccgcta cgacgaaac 
        caggggcac gatgctgtc gcattttgc ttattacgt 
        cgatatggc gaacatgtt aacaacacc tttatcgga 
        ggaagtgtt gctggatga attgcttcg tcacttccc 
        cgaaagaat acagtcgtt gtgggtatt agagaaaat 
        ctgtaccac cactgatgc cgccgaatt cctggcagc 
        tcaaaaaat tcaggggca aaatccggg agagaaaac 
        caacatcgc tcccaggga acgaccccg cgcaacaga 
        ccaccagct gcgtaacga gccaaagcg cccgcggcc 
        aaacattga ccgccacga agtaactcg tcctgcggc 
        ccctgcaaa tcgtggaaa tggtttcca aaaacgctc 
        aagtgacaa ccaacaatg gcgcaacaa ccaacccag 
        caccagcaa caaccgttg gcacaacaa caacgagag 
        caacagcaa caatcactg gcgcaacaa ccaacccag 
        caccagcaa caaccgttg gcacaataa caacggaaa 
        caacagcaa caatcactg gcgcagaaa caaccccaa 
        cgacaaccg caacaacac aagttcgag agcgcaaga 
        acggggcct aaggtactc cggccagca gcacggccc 
        acaaagcga cagcaggaa ccaacaatt gaggacggg 
        agagaaacg taaacgttc aacttcgtc acaatcaac 
        agaagggga aaacgatgc tcgaccgcg agcatccca 
        ggttaagtt aaatgtttt ttctataat taaatccgc 
        tgtcgcagg taacaacaa caaacagag ggtcgtgtc 
        ggagattaa gatccaaat ctcccaaca acaatcagt 
        caccgcgct tttggacat cggaagtga agtcagctg 
        tatttccga agaagtttg gtccaagct gatcgagac 
        cggaaacaa accgcccac gttaccggt tacctcaat 
        ccatcttcg tggagccat cggccagcg aagttgcca 
        gccaaccat tttgggagc agactggct aaatgagca 
        aggcgcggt catcgattt cgaaaacaa caaaatccc 
        tttgagaaa cggggagaa gcaccacag ttttccgtt 
        aagaaaaac cacggagat accaccgga accggagga 
        ttatgtgga agatcttgt tggtcacat cgaagtcaa 
        tcattcgac gccgatcac gacttttga acgcctaac 
        tcgatggac tttgatact aggacagta cgactacga 
        aatcgagtt ggtaaaagg aaaggataa tgtagttgc 
        cgacatcct gtcacgata cccgtccga cggtgaagc 
        cagttatga atatccacg tgaacaacc aatagttgc 
        aatgtttga agtcaacaa cacacctga aattctcca 
        actgatgtc cgatctcaa acaacacca atcagatga 
        tcctgtttt gagtcaaat aattgccaa caaacaatc 
        cggtgcagc tgctccaga tgcgagaac tcaacgggt 
        ttggtccca atataattt cacaaataa tgtgttagt 
        tcacagatc tcaaaattc aaacgacgt ttgcctggc 
        catacctga gagtttggt aattaagct cgtggatta 
        ccatcacca actcctggg ccatttcgg ggcaaccaa 
        agtgtacaa ttccatgaa gcgcgagta ctactggcc 
        gaacatgta tcgtacaat taagaaacg tctacgaag 
        ttgtgacct ttgtcagaa gacaaaaag ttccaaccg 
        tccacatca gggtccatt aacgccaat cctttacga 
        ccacattgg agacctagt ttgcgtcga tttctatgg 
        gcctttacc aacaggtcg attgggggc gtcctatgt 
        atttgtggt cattgacgt cttcagcaa gtttctcaa 
        actttaccc actgagaaa agccaccgc caaaatcgc 
        tgccaaacg tctcataga ggatttctc tgggtacat 
        caaacccaa atgtgtgtt aagcgacca cgggacaca 
        atttatttc gaacacttg gcagaatag tttgagagc 
        tgccgacat tcaaccaac gctctcgtc gattcgtca 
        cccagaatc gaaccctag cgagcgcgt aatgcggga 
        gctggggag aattttccg agcgtattg tagagaaaa 
        ccacgccag ttgggtcaa ccatttgag caacatcga 
        agattgttt gaattacgt gccacacat cagtaccgg 
        attttctcc atatgaaat cctgtacgg cagaacacc 
        accaaaccc actagatgc agtcacatc gggtttact 
        gcctgtacg tccgccact cacgagaga agaaattca 
        tgaaaaggc tctcgaaaa cttgagaca tcacgcgaa 
        cttacggca gaaaaacca aaagggtga agttaccat 
        actgaaaat taaagattg ggtcctact gaagaacaa 
        agtaacttc cgataccag aacccacca attcgccaa 
        gttcatgcc tttgtacag tggtccgta caaaataat 
        cgccaagcc tcatccaaa cacatacca aatcgccga 
        cccagttgt cgacatgga aaacaacag gaacaccgg 
        aaacgccgg agccccacc gcaagaaat gccgacgtg 
        gttcgttcg ttggttaaa ctcccaaca accaaggaa 
        tgtgccaaa cttcgcggc gccatcgac gtcgacagc 
        ggttcaaag accgcaagc catccttca ggtacgtcc 
        aagtaccag cgccgccgc tgatgtcga tggttggca 
        agtcactcc actacctag cgacgggac gacttcgcc 
        agaacccga cccggaaat tccggtagc tccggaacc 
        agcccctct tgcgagccc tcttgtgca agagccggg 
        aagttcgac cacatcagc gacttcggg agcagtgat 
        ggcctcacc acctatgcc aatcacaac agacaatgt 
        ggccgcaat ttccggagt actccggag cttgctgga 
        tcgcccact tccagctcc tgaacgacc atctttcga 
        aggcctgaa aaggcaaaa tccggtgaa attcttacg 
        agccattga agaatatgg acgtttttt cgggctcga 
        ctcgcagcg tcttttggg agtcgccat ggattgctt 
        gaaaggcaa tgccaaaca ttggacggg aatattcca 
        gaagaagtg gcgtggtta cgaggactt tcgacggga 
        ctttctccg gacctactg gtcggccaa gcgtcaacg 
        ggacatcag atttcaaat cgctacagg ccgctatga 
        cgaaaccag gggcacgat gctgtcgca ttttgctta 
        ttacgtcga catggcgaa catgttaac aacaccttt 
        atcggagga agtgttgct ggatgaatt actgcgtca 
        cttccccga aagaataca gtcgttgtg ggtattaga 
        gaaaatctg taccaccac ggatgccgc cgaattcct 
        ggcagctca agaaattcc gggacaaaa tccgggaga 
        gaaaaccaa catcgctcc cagggaacg accccgcgc 
        aacagacca ccagcggcg taacgagcc aaagcgccc 
        gcggccaaa cattgaccg ccacgaagt aactcgtcc 
        tgcggctcc tgcaaatcg tggaaatgg tttcccaaa 
        acgctcaag tgacgaaca acaatggcg caacaacca 
        acccagcac cagcaacaa ccgttggca caataacaa 
        cggaaacaa cagcaacaa tcactggcg caaaaacaa 
        ccccaacga caaccgcaa caacacaag ttcgagagc 
        gcagggacg gggcctaaa gtactccgg ccagcagca 
        cggcccaca aagcgacag caggaacca acaattgag 
        gacgggaga gaaacgtaa acgttcaac ttcgtcaca 
        gtcaacaga aggggaaaa cgatgctcg accgcgagc 
        atcccaggt taagttaaa tgttttttc tataattaa 
        atccgctgc cgcaggtaa caacaacat acaaagggt 
        cgtgccgga gattaagat ccaaatctc tcaacaaca 
        atcagtcac cgcactttt ggacaccgg aagtgaagt 
        cagctgtat ttccgaaga agtttggtc caagttgat 
        cgagaccgg aaacaaacc gcccacatt accggttac 
        ctcaatcca tcttcgtgg agccatcgg ccagcgaag 
        ttgccaggt tgttattca gtgttacct ggaaatcaa 
        aatcgacga acatctcta ccccgtcgt ggcgcttgt 
        cgttaaaaa tttaatcaa gccaaccat tttgggagc 
        agactggct aaatgaaca acgcgcggt catcgattt 
        cgataacaa cgaaatcct tttgagaaa cggggagaa 
        gcaccacag ttttccgtt tagaaaaac cacggagat 
        accaccgga accggagga ttatgtgga agatcttgt 
        tggtcacat cgaagtcaa tcattcggc gccgatcac 
        gacttgtaa caaaaagca acacttaga gtcacccag 
        tgccctaca agccaaggt ggacccatt aaaaattcc 
        tgaggccga gaaacggaa attaataca cctgctgca 
        agagtacag atgtatctt tagtttgcg tccagggct 
        aacacacaa atacactca cgaaataaa actacacga 
        caagactcc cttcttgaa acgaccgta cccagtacc 
        ttttgctct acgtcctgc cgtcgacgc cacaattca 
        ggaaatgtt agacttggg ggtgataaa acgtgaggc 
        gtcacctta cgcaagtcc catgacggt agtgaaaaa 
        gaaagatgg aacggtgag aatttgcct cgacgcccg 
        aatgatcaa ttccaaaat gatcgccga ttgtgagtc 
        accacccgc tgctgacga attattacg tcgatttca 
        cggaattcg ctacatgtc caccattga tttgagatc 
        gtcgtactg gcaaatccc actttcgcc agaaagcag 
        acagtacac cgcctttct gtacaacgg tcgcagtta 
        cacatacca agtcctacc tttcgggtt aaaaactgc 
        tgtgggttc attcagtcg tgcaatgga cgtagtgtt 
        aggaacaga agttcgcga attcgtcgt gaattatat 
        cgacgattt gttagtggc ttccgaaac gctaaacga 
        acatttaga acatctccg acaagtttt cgaaaaatt 
        gaagcaagc tagaatgac gataaatct cgaaaaatc 
        caatttcat acaaaagga accgacccg gaaaaaaat 
        cagcgccat ccgaaattt tccggttcc ccagaaaac 
        taaacacgt tcgagcctt cctgggact ttgtaattt 
        ttatcgaaa attttgtgc tcgatacag tgcagccac 
        acaagacgt aaacaaact acttcgaaa gggtgaaaa 
        atggagatg gggacgaaa tgaacaaga agctttcga 
        tcgtgtgaa agatctgtt cctcgaagc tgtgttgtt 
        acgttaccc cgacctgaa taaaatatt ctacgtcca 
        aactgacag ttctggtta cggattggg ggcggaatt 
        gtatcaaat tcaggaaga cggttcgcg aggtgtgat 
        cgcttttgc aagtcgctc tttgaaagg acccgaact 
        caactacac gactacaga aaaggaatt actcggcgt 
        catttttgc cttacacaa atttcgaat ctacatcca 
        agtcacaaa aatcatcat ccgaaccga ccaccaagc 
        tttgaaatt tctgagccg ttgtcgttt gctcagtga 
        acgcctaac tcgatggac tttgatact aggacagta 
        cgactacga aatcgagtt ggtaaaagg aaaggataa 
        tgtaattgc cgacatcct gtcacgata cccgtccga 
        cggtgaagc cagttatga atatccacg tgaacaacc 
        aatagttgc aatgtttga agtcaacaa cacacctga 
        aattctcca actgatgtc cgatctcaa acaacacca 
        atcagatga tcctgtttt gagtcaaat aattgccaa 
        caaacaatc cggtgcagc tgctccaga tgcgagaac 
        tcaacgggt ttggtccca atataattt cacaaataa 
        tgtgttagt tcacagatc tcaaaattc aaacgacgt 
        ttgcctggc catacctga gagtttggt aattaagct 
        cgtggatta ccatcacca actcctggg ccatttcgg 
        ggcaaccaa agtgtacaa ttccatgaa gcgcgagta 
        ctactggcc gaacatgta tcgtacaat taagaaacg 
        tctacgaag ttgtgacct ttgtcagaa gacaaaaag 
        ttccaaccg tccacatca gggtccatt aacgccaat 
        cctttacga ccacattgg agacctagt ttgcgtcga 
        tttctatgg gcctttacc aacaggacg attgggggc 
        gtcctatgt atttgtggt cattgacgt cttcagcaa 
        gtttctcaa actttaccc actgagaaa agccaccgc 
        caaaatcgc tgccaaacg tctcataga ggatttctc 
        tgggtacat caaacccaa atgtgtgtt aagcgacca 
        cgggacaca atttatttc gaacacttg gcagaatag 
        tttgagagc tgccgacat tcaaccaac gctctcgtc 
        gattcgtca cccagaatc gaaccctag cgagcgcgt 
        aatgcggga gctggggag aattttccg agcgtattg 
        taaagaaaa ccacaccag ttgggtcaa ccatttgag 
        caacatcga agattgttt gaactacgt gccacacat 
        cagtaccgg attttctcc atatgaaat cctgtacgg 
        cagaacacc accaaaccc actagatgc agtcacatc 
        gggtttact gcctgtacg tccgccact cacgagaga 
        agaaattca tgaaaaggc tcgcgaata cttgagaca 
        tcatgcgaa cttacggca gaaaaacca aaagggtga 
        agttaccgt actggaaat tgaagattg ggtcctact 
        gaagaacaa agtgacttc cgataccag aacccacca 
        attcgccaa gttcatgcc tttgtacag tggtccgta 
        caaaataat cgccaagcc tcatccaaa cacatacca 
        aatcgccga cccagtgac gaacgaaat taaaggtgt 
        atacaacat gacaaattt gaaattcta ccaccgacg 
        agacgacta aaataaaac cgcaagaag ttc